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The Interaction Between Recombinant Protein F Derived from Nontypeable Haemophilus influenza and Lipoprotein(a)
Liu En,
Li Weng-long,
Han Run-lin
Issue:
Volume 3, Issue 6, November 2015
Pages:
338-343
Received:
3 December 2015
Published:
5 December 2015
Abstract: Protein F (PF) is a surface plasminogen (Plg) receptor on the nontypeable Haemophilus influenza (NTHi). Plg via its lysine binding sites (LBS) can bind to PF. Apolipoprotein(a) [Apo(a)] is one component of Lipoprotein(a) [Lp(a)]. It has Kringle (K) domains, which contain LBS and has a high homology with Plg. Therefore, we speculated that Lp(a) might bind to Plg receptor on the surface of NTHi, subsequently competitively inhibiting the interaction of NTHi with Plg. In this study, recombinant PF (rPF) and its C-terminal lysine residue-deleted variant (rPFΔK) were expressed in E. coli BL21. The interactions of rPF with Plg and Lp(a) were tested by ELISA. The results showed that rPF could bind to Plg and Lp(a). The binding capacity of rPF was significantly higher than that of rPFΔK. The interactions of rPF with Plg and Lp(a) could be inhibited by EACA. 2 mmol/L of EACA significantly inhibited the binding of rPF to Plg, while 0.2 mmol/L of EACA could significantly reduce the binding of rPF to Lp(a). 50 ng/100 μL Lp(a) could significantly inhibit the interaction of rPF with Plg. In addition, affinity chromatography assay followed by Western biotting was also used to study the interaction. In overall, C-terminal lysine residue of rPF and the lysine binding sites (LBS) of Plg and Lp(a) should be responsible for these specifically bindings. Lp(a) could combine with rPF consequently inhibiting the interaction of Plg with rPF. This revealed that Lp(a) might play a role in anti-NTHi infection.
Abstract: Protein F (PF) is a surface plasminogen (Plg) receptor on the nontypeable Haemophilus influenza (NTHi). Plg via its lysine binding sites (LBS) can bind to PF. Apolipoprotein(a) [Apo(a)] is one component of Lipoprotein(a) [Lp(a)]. It has Kringle (K) domains, which contain LBS and has a high homology with Plg. Therefore, we speculated that Lp(a) migh...
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Study of Hematological Parameters and Risk Factors for Febrile Seizure Among Sudanese Children
Esam-eddin Bakhei Mohamed Ahmed,
Nagah Abd Elwehab Ahmed Mohamed,
Faroug Bakheit Mohamed Ahmed,
Mashair Abdelgadir,
Safa Ahmed Ali
Issue:
Volume 3, Issue 6, November 2015
Pages:
332-334
Received:
10 October 2015
Accepted:
26 October 2015
Published:
7 December 2015
Abstract: Current study aims to compare hematological parameters between children with febrile seizures and to find their common etiology among Sudanese children. In a case control study 150 children who were admitted to Gafar Ibn-Ouf pediatrics specialized hospital and Omdurman emergency pediatrics hospital during the period from Jun- Aug 2015 were studied. This study showed low levels of all hematological parameters in febrile seizures patients with exception of red distribution width (RDW), platelets and total iron binding capacity (TIBC) compared to their reference copartners. This evidence can be regarded as a risk factor for seizures in febrile children. The study also detected that children with low hematological levels of these parameters had a family history of febrile seizures and the consanguinity was associated with higher peak body temperature.
Abstract: Current study aims to compare hematological parameters between children with febrile seizures and to find their common etiology among Sudanese children. In a case control study 150 children who were admitted to Gafar Ibn-Ouf pediatrics specialized hospital and Omdurman emergency pediatrics hospital during the period from Jun- Aug 2015 were studied....
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An Unusual Cause of Low Back Pain: Uterine Conditions
Yunus Durmaz,
Ilker Ilhanli,
Kıvanc Cengiz,
Murat Gul
Issue:
Volume 3, Issue 6, November 2015
Pages:
335-337
Received:
12 November 2015
Accepted:
25 November 2015
Published:
7 December 2015
Abstract: The etiology of low back pain is often thought to include musculoskeletal disorders, such as lomber disc herniation or strain.Less commonly, low back pain is thought to refer from viscera.Sources of pain referred to the low back in females include uterine conditions.We aimed to investigate the female population of a rheumatology clinic to define the rate of uterine conditions that a rheumatologist can meet, and explore the possible association between uterine conditions and low back pain. Clinical data and imaging findings of 320 female patients who admitted to rheumatology clinic with the complaint of low back painwere retrospectively reviewed. Uterine myoma was detected in 20 patients (6,25%), and uterus retroversion was detected in only 3 patients (0,93%). Any reason of low back pain, except uterine myoma could be found in 4 of 20 patients.Patients did not meet the criterias for any of the inflammatory or mechanical causes of low back pain including fibromyalgia syndrome, and their sedimentation and CRP rates were normal, too.Low back pain which was explained by uterine anomaly rate was 2,18%.All 7 patients with uterine anomaly were complaining of low back pain with mechanical characteristics. Uterine myoma and uterus retroversion should be kept in mind by the physicians who meet with female patients at premenopausal ages suffering from low back pain after excluding the common causes of this condition.
Abstract: The etiology of low back pain is often thought to include musculoskeletal disorders, such as lomber disc herniation or strain.Less commonly, low back pain is thought to refer from viscera.Sources of pain referred to the low back in females include uterine conditions.We aimed to investigate the female population of a rheumatology clinic to define th...
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Immune-Related Gene Spatzle4 and Its Differential Immune Responses against Microbes in the Silkworm, Bombyx Mori
Ying Xu,
Ji Liu,
Fengpeng Li,
Xuefang Wang,
Xihai Li,
Zhongyuan Shen,
Jinmei Wu
Issue:
Volume 3, Issue 6, November 2015
Pages:
344-349
Received:
11 December 2015
Published:
11 December 2015
Abstract: Spatzle is a key factor in Toll signaling pathway against microbe invasion. The spatzle4 gene from silkworm (Bmspz4) was successfully cloned in this study, it is the second cloned and studied gene in the Spz family besides Bmspz1 in the silkworm and it was cloned for the first time in silkworm integument. The spatzle4 gene expression was analyzed in different tissues of the third day fifth instar larvae and the highest expression was detected in the head, and secondly in integument. Microbe infection showed that BmSpz4 participated in immune response. The transcriptional expression of BmSpz4 was induced differentially between Gram-negative bacteria and Gram-positive bacteria or fungi. This study showed that Bmspz4 gene plays an important role in the innate immunity of integument of silkworm, Bombyx mori.
Abstract: Spatzle is a key factor in Toll signaling pathway against microbe invasion. The spatzle4 gene from silkworm (Bmspz4) was successfully cloned in this study, it is the second cloned and studied gene in the Spz family besides Bmspz1 in the silkworm and it was cloned for the first time in silkworm integument. The spatzle4 gene expression was analyzed i...
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Caring Style Mediation a New Form of Medical Dispute Alternative Dispute Resolution in Taiwan
Shih-Ying Lee,
Yong-Chi Chen
Issue:
Volume 3, Issue 6, November 2015
Pages:
350-354
Received:
11 December 2015
Published:
11 December 2015
Abstract: This article aims to discuss new style medical mediation-Caring Style Mediation (CSM) a form of alternative dispute resolution (ADR). First, a description of current medical dispute mediation in Taiwan, 2nd, the contents of CSM were introduced. Then, we discoursed the introduction of Japanese’s style Internal Hospital Mediation into Taiwan and subsequently developed into CSM. Finally, we discuss the future promise of CSM in medical dispute and Safety and Quality of medical care.
Abstract: This article aims to discuss new style medical mediation-Caring Style Mediation (CSM) a form of alternative dispute resolution (ADR). First, a description of current medical dispute mediation in Taiwan, 2nd, the contents of CSM were introduced. Then, we discoursed the introduction of Japanese’s style Internal Hospital Mediation into Taiwan and subs...
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Efficacy of Commercially Available Anthelmintics in Controlling Gastrointestinal Nematodes in Goats Managed Under Natural Conditions in the South Western Highlands of Uganda
Katali Kirungi Benda,
Andrew Ampaire,
Jenninah Komungyeyo,
Robert Mukiibi,
Charles Masembe,
Robert Onzima
Issue:
Volume 3, Issue 6, November 2015
Pages:
355-363
Received:
20 December 2015
Published:
21 December 2015
Abstract: Goat production is of great importance especially to the rural farmers in Uganda, serving a number of socio-economic functions. Among the limitations of goat production in Uganda are Gastro Intestinal Nematodes (GINs). These are usually controlled using commercial Anthelmintic compounds. Due to continual improper usage of these drugs by the farmers, evolution of resistant strains has resulted thus affecting their effectiveness in controlling the GINs. The aim of this study was to determine the efficacy of four commercially available anthelmintics used for controlling Gastro Intestinal Nematodes (GINs) in goats on natural pastures in the south western highlands of Uganda. A mini survey was conducted prior to the current study to get information about the most commonly used anthelmintics in the study area. Initially, 240 goats were enrolled into the study and, 210 were used in the final analysis. The goats were randomly allocated to 5 different treatment groups at district level namely; untreated controls (CTRL), Albendazole 10% (ABZ), Ivermectin (IVE), Levamisole (LEV) and Closantel (CL). Faecal and blood samples were collected from each animal at 0, 14, 28 and 42 days post-treatment respectively and analysed for faecal egg counts (FEC) using the modified McMaster method. The percentage reduction, FECR % was calculated from the arithmetic mean and difference in FEC between breeds, district and age were assessed by univariate analysis of variance in SPSS. Results at day zero indicated that age and district had significant effect on FEC (P-value= 0.001 and 0.035respectively) while breed had no significant effect (P-value=0.465). At 14 days post-treatment, district, breed and treatment had significant effects on FEC (P-value=0.00, 0.02 and 0.05 respectively) while age was not (P=0.931). Albendazole and Levamizol were the most used drugs in the study area while Closantel and Ivermectin were the least used. Drug efficacy varied between districts, with the highest mean Fecal Egg Count Reduction (FECR) achieved with Closantel (FECR%=100%), while the lowest mean FECR observed with Albendazole (FECR%=49%) in Rukungiri and Kisoro districts respectively. Ivermectin was effective in all the four districts (FECRT% > 95%). Our study confirmed presence of Anthelmintic resistance (AR) by GIN in goats in the south western highlands of Uganda. Closantel is highly effective against GINs, but albendazole is not due to its wide spread usage. Further research is necessary to find and validate alternative strategies for the control of GINs in goats.
Abstract: Goat production is of great importance especially to the rural farmers in Uganda, serving a number of socio-economic functions. Among the limitations of goat production in Uganda are Gastro Intestinal Nematodes (GINs). These are usually controlled using commercial Anthelmintic compounds. Due to continual improper usage of these drugs by the farmers...
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Detection of Helicobacter pylori and Human Papillomavirus in Peroperative Tissue Biopsies Collected from Malignancies in Oropharyngeal Area
Emil Pavlik,
Eva Nartova,
Jaromir Astl,
Barbora Drnkova,
Petr Lukes,
Bela Potuznikova,
Rami Katra,
Jaroslav Kraus,
Ivan Sterzl
Issue:
Volume 3, Issue 6, November 2015
Pages:
364-367
Received:
24 December 2015
Published:
30 December 2015
Abstract: Helicobacter pylori has been reported as pathogen of human GIT. It is associated with type B gastritis and peptic ulcers. Bacterium´s relationship to cancer has also been declared and H. pylori considered cancer-inductor. A number of studies documented H. pylori residence in oropharynx, generating hypotheses on participation in development of cancer in oropharyngeal area. Human papillomaviruses are DNA viruses colonizing skin and mucoid membranes of the host. Their oncogenic potential, especially in genitourinary system, has been confirmed. High-risk type HPV16 (group A9) is frequently reported as cancer-inductor in oropharyngeal area. The aim of this study is to contribute to discussions on induction of malignancies in oropharyngeal area, providing comparison of incidence of one bacterial and one viral pathogen in the cells and tissues of oropharyngeal neoplasia. Using real-time PCR-based tests, we investigated 70 tissue specimens collected during cancer surgery for detection of bacterial DNA of Helicobacter pylori and viral DNA of High risk HPV (groups A9, A7 and A5/6). Results: Helicobacter pylori DNA was detected in 60 samples (85.7%), while DNA of HPV only in 42 (60%). If focused on HPV-16 as proposed cancer inductor, it was detected in 34 samples (48.5%) only. No DNA of respective agents was detected in 7 samples (10%). There were 21 Helicobacter sole pathogen detections compared with only 3 of HPV. Conclusions: There is no doubt, Helicobacter pylori is a long-term resident in oropharynx and tonsils. This residence most likely influences functions of immune system, so that a newly entering contributor could switch-on the process resulting in cancer development. This could support high incidence of common detection of HPV and Helicobacter pylori in 39 samples (55.7%).
Abstract: Helicobacter pylori has been reported as pathogen of human GIT. It is associated with type B gastritis and peptic ulcers. Bacterium´s relationship to cancer has also been declared and H. pylori considered cancer-inductor. A number of studies documented H. pylori residence in oropharynx, generating hypotheses on participation in development of cance...
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Pharmacokinetics and Relative Bioavailability of Ambroxol Hydrochloride Aerosol and Injection
Jing Zhang,
Na Yi,
Lidao Bao,
Tengfei Yu
Issue:
Volume 3, Issue 6, November 2015
Pages:
368-371
Received:
24 December 2015
Published:
30 December 2015
Abstract: To compare the pharmacokinetics and relative bioavailability of ambroxol hydrochloride (AH) aerosol and injection, the volunteers were subjected to single-dose crossover inhalation (injection) of 100 mg AH aerosol (injection). The drug concentrations in plasma were determined by HPLC. The areas under curve (AUC) of the two formulations were compared by the three-factor analysis of variance and the bi-directional one-side t test. The Cmax, tmax, t1/2β and AUC values of the aerosol group and the injection group were (154.75±26.12) and (157.39±26.09) ng/ml, (1.12±0.34) and (1.29±0.33) h, (6.98±1.62) and (7.75±1.26) h, and (1593.02±290.45) and (1438±132.46) h•ng/ml, respectively. The bioavailabilities of AH aerosol and injection were the same, and the relative bioavailability of the aerosol was (96.52±11.44)%.
Abstract: To compare the pharmacokinetics and relative bioavailability of ambroxol hydrochloride (AH) aerosol and injection, the volunteers were subjected to single-dose crossover inhalation (injection) of 100 mg AH aerosol (injection). The drug concentrations in plasma were determined by HPLC. The areas under curve (AUC) of the two formulations were compare...
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Mongolian Medical Acupuncture Improves Sleep Quality in Patients with Primary Insomnia
Lengge Si,
Lidao Bao,
Rui Peng,
Yuehong Wang,
Agula B
Issue:
Volume 3, Issue 6, November 2015
Pages:
372-377
Received:
24 December 2015
Published:
30 December 2015
Abstract: The aim of this study was to evaluate the therapeutic effects of the Mongolian medical warm needle acupuncture (MMA) in treating patients with primary insomnia. 40 patients with primary insomnia were randomly divided into 2 groups as (1) Control Group, who received automatic neural balance regulation (ANBR), and (2) MMA Group, who received ANBR plus MMA treatment. The MMA treatment was administered to 5 acupuncture points according to traditional Mongolian medicine. Pittsburgh Sleep Quality Index (PSQI) was used to quantitatively measure patients’ outcome at time 0 (prior to study involvement), time 1 (after 8-week treatment), and time 2 (follow-up examination 4-week post-treatment). Multivariate analyses were conducted using treatment, gender, time, and age as factors and covariates. Cronbach’s alpha coefficient was used to evaluate internal homogeneity. MMA significantly reduced PSQI in insomniac patients compared with control (t = 9.59, p < 0.001). Six component scores of the PSQI were internally consistent (Cronbach’s alpha coefficient = 0.89). Out of the 6 components of PSQI, MMA significantly improved subjective sleep quality, sleep latency, and daytime dysfunction. The Mongolian medical warm needle acupuncture combined with automatic neural balance regulation has significant therapeutic effects in treating primary insomnia.
Abstract: The aim of this study was to evaluate the therapeutic effects of the Mongolian medical warm needle acupuncture (MMA) in treating patients with primary insomnia. 40 patients with primary insomnia were randomly divided into 2 groups as (1) Control Group, who received automatic neural balance regulation (ANBR), and (2) MMA Group, who received ANBR plu...
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Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer
Na Yi,
Lengge Si,
Yuehong Wang,
Lidao Bao
Issue:
Volume 3, Issue 6, November 2015
Pages:
378-382
Received:
24 December 2015
Published:
30 December 2015
Abstract: To observe the effects of Apigenin on the expressions of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of mice with H29 colon cancer. Fifty ICR mice with H29 colon cancer were randomly divided into five groups: normal saline group, low-dose Apigenin group, middle-dose Apigenin group and high-dose Apigenin group and cyclophosphamide group. The mice were killed on the next day of administration discontinuance, and subcutaneous tumor tissues were collected. Quantitative fluorescence RT-PCR was used to detect the expression of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of H29 colon cancer mice. Apigenin raised the expression level of TGF-β1R II in H29 colon cancer tissues, which showed the most obvious effect in the middle-dose group, with a significant difference compared with the normal saline group (P<0.01). The Apigenin group of each dose could significantly lower the NF-κB expression level in H29 colon solid tumors, showing significant differences compared with the normal saline group (P<0.01). The middle-dose and high-dose Apigenin groups could significantly reduce the level of VEGF expression in tumor tissues of ICR mice with H29 colon cancer, and the high-dose group had most obvious effect, and there were significant difference among the middle-dose group, high-dose group and the normal saline group (P<0.01). The mechanism of anti-tumor effect of Apigenin might be the reason that Apigenin can raise the expression level of TGF-β1R II by down-regulating the expression of NF–κB and VEGF in tumor tissues of tumor-bearing mice, thereby inhibiting tumor angiogenesis and tumor cell proliferation, so as to achieve the anti-tumor effect.
Abstract: To observe the effects of Apigenin on the expressions of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of mice with H29 colon cancer. Fifty ICR mice with H29 colon cancer were randomly divided into five groups: normal saline group, low-dose Apigenin group, middle-dose Apigenin group and high-dose Apigenin group and cyclophosphamide group. The m...
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Iatrogenic Invasion of Maxillary Sinus in Oral Surgery
Antonio Crispino,
Leonzio Fortunato,
Luigi Lidonnici,
Roberto Del Giudice
Issue:
Volume 3, Issue 6, November 2015
Pages:
383-385
Received:
30 December 2015
Published:
30 December 2015
Abstract: Iatrogenic diseases affecting the maxillary sinus may be consequent to both technical errors operator both represent something "inevitable", linked to the particular situation of anatomical contiguity between pathologic finding to be removed and the sinus. They can result from multiple occurrences: Extraction of dental elements erupted of the posterior maxilla, most frequently in the case of multi-rooted elements with roots long and diverse; Surgical extraction of impacted teeth, especially the third molars, second premolars and, more rarely, canines; Dislocation in the roots of the maxillary sinus, dental elements or parts of fractured instruments (in this case the event is always tied to a technical error); Enucleation of periapical lesions or cysts whose walls are adherent to the sinus mucosa; Removal of benign growths such as odontomas or other odontogenic tumors and odontogenic not directly related to the sinus mucosa; Preparation of implant sites in the posterior maxilla, due to incorrect assessment of the space available between the alveolar margin and sinus floor. Meta-analytical, observational and retrospective study obtained from a literature review of 10 articles. Its primary etiological factor is dental extraction and preparation of dental implant sites. The lesions iatrogenic sinus occurred during oral surgery or implant surgery can be provided and certainly reduced. The way forward is that of a careful planning designed to reduce the risk of accidents during surgery.
Abstract: Iatrogenic diseases affecting the maxillary sinus may be consequent to both technical errors operator both represent something "inevitable", linked to the particular situation of anatomical contiguity between pathologic finding to be removed and the sinus. They can result from multiple occurrences: Extraction of dental elements erupted of the poste...
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