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Anti-Inflammatory Effect of Aloe Vera (l) Burm, F. on COX-2 and Histamine Expression in Mice Skin Exposed to UVB
Venyce Laurence Agung,
Alwi A. Mappiasse,
Siswanto Wahab,
Gemini Alam,
M. Husni Cangara,
Ilhamjaya Patellongi
Issue:
Volume 4, Issue 4, July 2016
Pages:
94-97
Received:
20 May 2016
Accepted:
28 May 2016
Published:
13 June 2016
Abstract: The purpose of this study was to investigate the anti-inflammatory effect of topical aloe vera on COX-2 and histamine expression in mice skin exposed to UVB. COX-2 and histamine expression were examined by using immunochemistry staining in skin samples from totally 20 Swiss Albino mice in the control group, UVB group, UVB+Ethanol group, UVB+Aloe group. The study show topical application of aloe vera with a concentration of 200 mg/kg body weight suppress expression of COX-2 in mice skin exposed to UVB, however it have not been proven to reduce histamine expression. Aloe vera with a dose of 200mg/kg body weight has show anti-inflammatory effects by reduction COX-2 expression that assessed 24 hours after the last treatment.
Abstract: The purpose of this study was to investigate the anti-inflammatory effect of topical aloe vera on COX-2 and histamine expression in mice skin exposed to UVB. COX-2 and histamine expression were examined by using immunochemistry staining in skin samples from totally 20 Swiss Albino mice in the control group, UVB group, UVB+Ethanol group, UVB+Aloe gr...
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The Level of Copper in Hair Androgenic Alopecia
Wiwiek Amriyana Saputri,
Farida Tabri,
Nurelly Noro Waspodo,
Burhanuddin Bahar,
Agussalim Bukhari,
Nursiah La Nafie
Issue:
Volume 4, Issue 4, July 2016
Pages:
98-102
Received:
20 May 2016
Accepted:
31 May 2016
Published:
17 June 2016
Abstract: Androgenic alopecia is characterized by progressive loss of hair from the scalp. This research aimed to determine the hair and blood copper levels in men with androgenic alopecia. The research was conducted in the Department of Dermatovenereology of Dr. Wahidin Sudirohusodo Hospital, Makassar and the Center for Health Laboratory, Makassar, using the observational research method. The samples comprised 21 the males with androgenic alopecia and 11 control samples without androgenic alopecia. The hair and blood of the samples were analyzed using the atomic absorption spectrophotometer receipts. The research results indicated resources that the hair copper level in the androgenic alopecia group Showed a difference compared to the hair copper levels in the control group, though the difference was significant (p <0.05). On the other hand, the blood copper levels in androgenic alopecia group had no difference compared to that in the control group. The hair copper levels had no correlation with the blood copper levels. The age had a significant correlation with the androgenic alopecia, in that, the alopecia incidence would increase of as the age increased (p <0.05). However, the levels of both the hair and blood coppers had no correlation with the age. Neither, did the levels of both copper hair and blood have a correlation with the degree of androgenic alopecia (Hamilton grade).
Abstract: Androgenic alopecia is characterized by progressive loss of hair from the scalp. This research aimed to determine the hair and blood copper levels in men with androgenic alopecia. The research was conducted in the Department of Dermatovenereology of Dr. Wahidin Sudirohusodo Hospital, Makassar and the Center for Health Laboratory, Makassar, using th...
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Relationship Between Overexpression of EML4-ALK Variant 1 and Inflammatory Moleculars and Immune Mediators Associated with Tumor Progression and Metastasis in BEAS-2B and H2126 Cells
Issue:
Volume 4, Issue 4, July 2016
Pages:
103-108
Received:
16 June 2016
Published:
17 June 2016
Abstract: The aim of this study was to investigate the relationship between overexpression of EML4-ALK and inflammatory factors about tumor progression and metastasis in a human bronchial epithelial cell (BEAS-2B) and a lung cancer cell (H2126). The recombinant plasmids with EML4-ALK variant 1 and EML4-ALK K589M (EML4-ALK variant 1 kinase inactive mutant) fusion gene were constructed and introduced into H2126 and BEAS-2B cells after transfection. The plasmid pcDNA3.1 was negative control. Subsequent, cell proliferation assay and scratch wound healing assay were used to examine the proliferation and invasion of BEAS-2B and H2126 cells after transfection. Finally, we analyzed 24 inflammatory moleculars and immune mediators associated with tumor progression and metastasis. Compared to the empty vector as control, the expression level of ALK was upregulated in BEAS-2B and H2126 cells after transfection the plasmid EML4-ALK variant 1 and plasmid EML4-ALK K589M. In vitro, EML4-ALK variant 1 promoted the proliferation and invasion ability of BEAS-2B and H2126 cells compared with EML4-ALK K589M and empty vector. The results of Q-PCR showed that factors more differentially expressed between both groups of BEAS-2B and H2126 cells were S100A8 and S100A9 after transfection EML4-ALK variant 1. In conclusion, an increased expression level in S100A8 and S100A9 by overexpression EML4-ALK variant 1 had a great biological interest because of their relation with tumor cell proliferation and migration.
Abstract: The aim of this study was to investigate the relationship between overexpression of EML4-ALK and inflammatory factors about tumor progression and metastasis in a human bronchial epithelial cell (BEAS-2B) and a lung cancer cell (H2126). The recombinant plasmids with EML4-ALK variant 1 and EML4-ALK K589M (EML4-ALK variant 1 kinase inactive mutant) fu...
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Catheterization Related Complications Among Adults Male Patients with Urinary Retention in a Teaching Hospital
Muzzammil Abdullahi,
Bashir Yunusa,
Sharfuddeen Abbas Mashi,
Sani Ali AJI,
Sani Usman Alhassan
Issue:
Volume 4, Issue 4, July 2016
Pages:
109-111
Received:
18 May 2016
Accepted:
30 May 2016
Published:
21 June 2016
Abstract: Urethral catheterization is a common procedure in urologic practice. It is most commonly employed in relieving urinary retention. Various complications are associated with urethral catheterization and these depend on availability of expertise and strict observation of asepsis during the procedure. This study aimed at establishing the types and prevalence of complications associated with urethral catheterization among patients with urinary retention. It was a prospective study on 110 patients who presented in urinary retention and who were catheterize to relieve the retention. On presentation the patients were catheterize and urine was immediately taken for microscopy, culture and sensitivity. Patients with established UTI were excluded from subsequent investigation of catheter related UTI. Other complications of catheterization were also looked for and recorded. The patients age ranged from 17 to 100 years, with a mean age of 56±19.3 SD years. Up to 74.5% of the patients in this study did not develop any of the complications. The most common complication was introduction of UTI in 17.3%. This was followed by haematuria in 5.5% of the patients. Three patients (2.7%) had their urethra injured and none had post obstructive diuresis. Catheterization related urinary tract infection is common despite observing asepsis in relieving urinary retention but other complications are not common.
Abstract: Urethral catheterization is a common procedure in urologic practice. It is most commonly employed in relieving urinary retention. Various complications are associated with urethral catheterization and these depend on availability of expertise and strict observation of asepsis during the procedure. This study aimed at establishing the types and prev...
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An Assessment of Renal Function Parameters on the Ameliorative Properties of Ginkgo Biloba Extract in Cadmium-Induced Nephrotoxicity in Adult Wistar Rats Model
Ogunnaike Philip Olubunmi,
Olatunji Sunday Yinka,
Owolabi Joshua Oladele,
Fabiyi Adetutu Olubusayo,
Olanrewaju John Afees
Issue:
Volume 4, Issue 4, July 2016
Pages:
112-117
Received:
25 May 2016
Accepted:
2 June 2016
Published:
21 June 2016
Abstract: Renal failure is a growing concern in the modern society and the increasing rate of exposure to toxic substances has contributed a lot to renal diseases. As a result of this, there is a great need for agents that can serve as nephroprotective. The present study assessed some parameters of renal function on the ameliorative properties of Ginkgo biloba extract (GBE) in cadmium (Cd) - induced nephrotoxicity in adult wistar rats model. The study was performed on twenty four (24) male Wistar rats divided into four groups: Group A (Control), Group B (Cd), Group C (Cd + 100mg/kg BW of GBE) and Group D (Cd + 300mg/kg BW of GBE). The Cd and GBE were administered orally through oro-gastric cannula. The results showed changes in the renal function chemical parameters (creatinine, urea, and total protein) in the treated groups compared to the control group. Exposure to Cd lowered the activities of the kidney by increasing the plasma level of creatinine and urea, and decreasing the plasma level of total protein. The creatinine concentration decreased significantly (p<0.05) in groups C and D while the urea concentration increased on GBE administration. A significant decrease was observed in the plasma total protein level in the Cd group when compared with the control and on administration of GBE there was an increase in the plasma total protein concentration in groups C and D. The results showed that Ginkgo biloba treatment ameliorated the effects of Cd-induced nephrotoxicity in all parameters except urea which is to be further investigated. These may be explained by the oxidative and anti-oxidative properties of the Cd and GBE respectively and their mechanisms of action.
Abstract: Renal failure is a growing concern in the modern society and the increasing rate of exposure to toxic substances has contributed a lot to renal diseases. As a result of this, there is a great need for agents that can serve as nephroprotective. The present study assessed some parameters of renal function on the ameliorative properties of Ginkgo bilo...
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