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Oxygen Ozone Therapy in the Treatment of Chronic Obstructive Pulmonary Disease: An Integrative Approach
Emma Borrelli,
Velio Bocci
Issue:
Volume 2, Issue 2, March 2014
Pages:
9-13
Received:
19 January 2014
Published:
10 March 2014
Abstract: A pilot study has been performed on fifty COPD patients: besides using effective drugs, half of the patients have been treated also with major ozonated autohaemotherapy . This treatment has been evaluated during the last two decades and is absolutely atoxic: the treated patients have shown a significant improvement of the six minute walking test and Saint George Respiratory Questionnaire total score. Surprisingly their quality of life was also much improved. Orthodox medications appears to be greatly potentiated when integrated by the autologous infusion of ozonated blood. This combination deserves to be evaluated in an ample clinical trial.
Abstract: A pilot study has been performed on fifty COPD patients: besides using effective drugs, half of the patients have been treated also with major ozonated autohaemotherapy . This treatment has been evaluated during the last two decades and is absolutely atoxic: the treated patients have shown a significant improvement of the six minute walking test an...
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The Reproducibility of the Multiplex RAPD-PCR Assay in Genotyping of Mycobacterium Tuberculosis Isolates from Sulawesi, Indonesia
Mochammad Hatta,
AndiRofian Sultan,
Ressy Dwiyanti,
Muhammad Sabir,
Andini Febrianty,
Ahmad Adhyka,
Nur Indah Purnamasari,
Muhammad Reza Primaguna,
Juhri Saning,
Yusriani Mangarengi,
Munawir Muhammad,
Nataniel Tandirogang,
Yadi Yasir,
Masyhudi Amir
Issue:
Volume 2, Issue 2, March 2014
Pages:
14-21
Received:
20 February 2014
Published:
20 March 2014
Abstract: Background: Random Amplified Polymorphic DNA (RAPD) assay has recently emerged as a genotyping method which is both robust and highly discriminatory for bacterial strain differentiation. However, RAPD assessment for Mycobacterium tuberculosiscomplex (MTC) isolates is still limited. Despite its simplicity and rapidity, conventional RAPD also has low reproducibility due to its sensitivity to several factors. Therefore we studied the feasibility of an RAPD-PCR assay to define the genetic diversity of MTC isolates and to evaluate its reproducibility.Methods:493clinical MTC isolates from the island of Sulawesi in Eastern Indonesia, collected from 2005-2012were subjected to Multiplex RAPD assay using 11 random decamer primers instead of one primer which is common in conventional RAPD. All 11 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for MTC. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers.Results:Multiplex RAPD-PCR results show that the majority of the isolates from South Sulawesi, Southeast Sulawesi andCentral Sulawesiin eastern region of Indonesia belong to group MT-C (80.7%, 80.0% and 62.6 % respectively) with result reproducibility as high as 100%. Conclusion:Molecular typing with multiplex RAPD-PCR is a powerful approach to show the genetic heterogeneity of MTC isolates. The discrimination power and reproducibility of this multiple loci-based RAPD was higher than conventional fewer loci-targeted RAPD.
Abstract: Background: Random Amplified Polymorphic DNA (RAPD) assay has recently emerged as a genotyping method which is both robust and highly discriminatory for bacterial strain differentiation. However, RAPD assessment for Mycobacterium tuberculosiscomplex (MTC) isolates is still limited. Despite its simplicity and rapidity, conventional RAPD also has low...
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Role of Vagus Nerve in Gastroduodenal Adaptation and Cytoprotection
Oksana Sulaieva,
Natalia Obraztsova
Issue:
Volume 2, Issue 2, March 2014
Pages:
22-27
Received:
18 March 2014
Accepted:
9 April 2014
Published:
20 April 2014
Abstract: Objective: In this review we focused on understanding the cause-and-effect relationships of gastroduodenal pathology aiming to clarify the role of vagus nerve. Results: The spectrum of vagus nerve biological effects in gastroduodenal area is related to its numerous targets and a wide range of its receptors. A variety of vagus nerve effects are related to the broad expression of cholinergic receptors on the target cells: smooth muscle cells, covering and glandular epithelium of stomach and duodenum, myofibroblasts and mast cells, vascular endothelium, intramural ganglion neurons, endocrine cells, platelets and blood leukocytes. In this paper, we discussed the following issues: 1) role of sensory nerve endings in the vagal reflex regulation; 2) impact of gastrin and leptin on vagal afferentation; 3) targets of vagus efferent nerves; 4) the role of acetylcholine in regulation of functional activity of oxyntic cells; 5) relationship of vagus efferents with enteroendocrine cells; 6) the role of vagus nerve in realization of compensatory and adaptive reactions in gastroduodenal area. Conclusion: Vagus nerve is one of the key regulators of mucosal activity and blood supply, modulating adaptive reactions and maintaining the gastrointestinal barrier
Abstract: Objective: In this review we focused on understanding the cause-and-effect relationships of gastroduodenal pathology aiming to clarify the role of vagus nerve. Results: The spectrum of vagus nerve biological effects in gastroduodenal area is related to its numerous targets and a wide range of its receptors. A variety of vagus nerve effects are rela...
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Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study
Azab El Saied Azab,
Fathy Ahmed Fetouh,
Mohamed Omer Albasha
Issue:
Volume 2, Issue 2, March 2014
Pages:
28-35
Received:
25 March 2014
Accepted:
10 April 2014
Published:
20 April 2014
Abstract: The kidney is a common target for toxic xenobiotics due to its capacity to extract and concentrate toxic substances by highly specialized cells and also, due to its large blood flow. Objective: The present work aimed to evaluate the effectiveness of different natural materials (curcumin, rosemary and propolis) against the histological and also biochemical alterations of gentamicin induced nephrotoxicity in guinea pigs. Materials and methods: 48 guinea pigs were used for this study and divided into 8 groups. The first 4 groups were control groups, the 5th group was the experimental and administered gentamicin at a dose of 100 mg/kg body wt for 10 days, and in the 6th , 7th , and 8th groups, gentamicin was co-administered with curcumin, rosemary, and propolis at the doses of 200 mg, 220 mg, and 100 mg/kg body wt respectively. The animals were sacrificed and the kidneys were dissected and specimens were obtained. The specimens were processed for light microscopic examinations. Blood samples were obtained for assessment of urea, creatinine and uric acid levels. Results: In gentamicin treated animals, there were structural changes. The proximal convoluted tubules showed degenerated epithelial lining with disruption of their brush borders and presence of epithelial debris inside their lumens. The renal corpuscle appeared with degeneration of the glomerulus and disrupted Bowman's capsule. The afferent arteriole showed thickening in its wall and degeneration of endothelial lining with extensive perivascular infiltration of inflammatory cells. Massive interstitial hemorrhage was seen. Also, the serum urea, creatinine, and uric acid were elevated. Co-administration of curcumin, rosemary, and propolis significantly improved the structural changes in the kidney and the blood urea, creatinine and uric acid were significantly declined. Conclusion: It can be concluded that, the gentamicin has adverse effects on the kidney. Different natural materials as curcumin, rosemary, and propolis were able to protect the kidney against these effects. So, the patients should be advised to take one of these materials while they are treated by gentamicin.
Abstract: The kidney is a common target for toxic xenobiotics due to its capacity to extract and concentrate toxic substances by highly specialized cells and also, due to its large blood flow. Objective: The present work aimed to evaluate the effectiveness of different natural materials (curcumin, rosemary and propolis) against the histological and also bio...
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Ovarian Conservation Versus Removal at the Time of Hysterectomy for Benign Gynecological Diseases
Tarafdar Runa Laila,
Sheikh Salahuddin Ahmed,
Khairun Nahar,
Parveen Akhter Shamsunnahar,
Sharmeen Mahmood,
Saleha Begum Chowdhury
Issue:
Volume 2, Issue 2, March 2014
Pages:
36-41
Received:
29 March 2014
Accepted:
9 April 2014
Published:
20 April 2014
Abstract: Hysterectomy for benign gynecological diseases is a common surgical procedure. Prophylactic bilateral oophorectomy is often recommended concurrent with hysterectomy to decrease the risk of ovarian cancer. Oophorectomy before menopause leads to an abrupt decrease in endogenous estrogen and androgen production leading to different health problems. So women undergoing hysterectomy for benign gynecological diseases are presented with the choice of ovarian conservation or removal. The purpose of this review article is to summarize and critically evaluate the existing evidences regarding the impact of ovarian conservation versus removal on specific health issues of the individual. Information was collected by searching pub med for related studies, abstracts and articles. Studies have shown that benefits of elective oophorectomy also include reduction of breast cancer and development of residual ovary syndrome. On the other hand bilateral oophorectomy is associated with increased risk of cardiovascular disease, osteoporosis, cognitive impairment, dementia, depression, anxiety and decreased sexual function. Estrogen was commonly prescribed after bilateral oophorectomy to treat menopausal symptoms. But recent studies have shown more harm than benefit in postmenopausal hormone therapy. So the decision making regarding elective oophorectomy at the time of hysterectomy for benign indications has become complex. The approach to such decision will include counseling regarding risks and benefits of the procedure and shared decision making between the clinician and the patient. The beneficial effect on ovarian cancer must be weighed against the risks of ovarian hormone withdrawal.
Abstract: Hysterectomy for benign gynecological diseases is a common surgical procedure. Prophylactic bilateral oophorectomy is often recommended concurrent with hysterectomy to decrease the risk of ovarian cancer. Oophorectomy before menopause leads to an abrupt decrease in endogenous estrogen and androgen production leading to different health problems. So...
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