Detection of Infectious Bovine Rhinotracheitis in Natural Cases of Bovine Abortion by PCR and Histopathology Assays
Farhang Sasani,
Aida Vazirian,
Javad Javanbakht,
Mehdi Aghamohammd Hassan
Issue:
Volume 1, Issue 2, September 2013
Pages:
35-39
Received:
29 July 2013
Published:
30 August 2013
Abstract: Bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine. Bovine Herpesvirus 1(BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. The objective of this study was to determine the relation between type of macroscopic lesions and bovine herpesvirus-1(BHV-1) infection; hence the aborted fetus specimens were initially prepared for PCR test. In this study, 23 of fetus samples, which were transferred to Veterinary Laboratories during 2009-2012, were assessed for BHV-1 with PCR in lung and spleen tissues and all of positive samples undergone histopathological study. The results indicated myocarditis and necrosis of cardiac tissue in 8 cases (42.1%), and 6 cases encompassed interstitial lymphocytic pneumonia (28.57%), whereas 5 cases revealed bronchopneumonia (23.8%) and in 8 cases (38.09%) severe interstitial edema was observed. In addition, the lesions may be associated with BHV-1, so that in brain tissue 9 cases (36 %) possessed encephalitis and necrosis, 8 cases (38.09%) indicated interstitial nephritis, 2 cases (9.52%) developed acute tubular necrosis, 2 cases (9.52%) revealed vacuolar degeneration inrenal tubular epithelium, 1 case had (4.76%) renal hemorrhage and in 3 cases (14.28%) renal autolysis was observable. In liver, 10 cases (47.61%) were affected to lymphocytic hepatitis, 5 cases (23.8%) possessed necrotic hepatitis, 6 cases (28.57%) of multifocal coagulative necrosis without inflammatory reaction and 3 cases (14.28%) with lymphocytic portal hepatitis were observed. In this assessment, 10 specimens (52.17%) of spleen tissues revealed necrosis and lymphatic tissues emptying and in 10 cases (100%) severe enteritis, which may be associated with IBR, were recorded. This study suggests based on lesions in several organs induced by IBR virus and real time PCR confirmation there is direct correlation between virus and lesions, and in order to obtain more reliable results of IBR infection the histopathological examination may be efficient.
Abstract: Bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine. Bovine Herpesvirus 1(BHV-1) belongs to the genus of Varicellovirus and the family of Herpesviridae which contains three main gB, gC and gD genes. The objective of this...
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Analysis of Oxidative DNA Damage / Oxidative Stress Markers in Patients with Ovarian Cancer
Issue:
Volume 1, Issue 2, September 2013
Pages:
40-43
Received:
21 August 2013
Published:
20 September 2013
Abstract: Although the role of oxidative stress in the process of carcinogenesis seems well known, the quantitative correlation between oxidative DNA damage and the degree of histological malignancy of a neoplasm has not yet been determined. In the present study we attempted to show the possible correlations by looking at the amounts of the basic oxidative stress markers 8-oxoGua (8-oxo-7.8-dihydroguanine) and 8-oxodG (8-oxo-7.8-dihydro-2’-deoxyguanosine) excreted in the urine of patients. All the previously mentioned modifications were analyzed using techniques involving high performance liquid chromatography/electrochemical detection (HPLC/EC) or HPLC/gas chromatography and mass spectroscopy (GC-MS). All patients (n=46) suffered from ovarian cancer and were divided into three groups: G1, G2, and G3, according to the degree of histological malignancy of the neoplasm. In the female patients whose neoplasms showed higher degrees of histological malignancy significantly higher median values of 8-oxoGua and 8-oxodG were found to be excreted in their urine. In the subgroup of patients with G3 feature, both these markers of oxidative stress were almost twice as high as in the subgroup with G1 feature. The results suggest that the oxidative stress in ovarian cancer patients as demonstrated by increased amounts of these modifications in urine may be typical not only for the affected tissue, but also for other tissues and even the whole organism. It is also possible that the accumulation of oxidative stress is proportional to the degree of histological malignancy and depends on histological diagnosis.
Abstract: Although the role of oxidative stress in the process of carcinogenesis seems well known, the quantitative correlation between oxidative DNA damage and the degree of histological malignancy of a neoplasm has not yet been determined. In the present study we attempted to show the possible correlations by looking at the amounts of the basic oxidative s...
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