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Detection of Helicobacter pylori and Human Papillomavirus in Peroperative Tissue Biopsies Collected from Malignancies in Oropharyngeal Area

Received: 24 December 2015     Published: 30 December 2015
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Abstract

Helicobacter pylori has been reported as pathogen of human GIT. It is associated with type B gastritis and peptic ulcers. Bacterium´s relationship to cancer has also been declared and H. pylori considered cancer-inductor. A number of studies documented H. pylori residence in oropharynx, generating hypotheses on participation in development of cancer in oropharyngeal area. Human papillomaviruses are DNA viruses colonizing skin and mucoid membranes of the host. Their oncogenic potential, especially in genitourinary system, has been confirmed. High-risk type HPV16 (group A9) is frequently reported as cancer-inductor in oropharyngeal area. The aim of this study is to contribute to discussions on induction of malignancies in oropharyngeal area, providing comparison of incidence of one bacterial and one viral pathogen in the cells and tissues of oropharyngeal neoplasia. Using real-time PCR-based tests, we investigated 70 tissue specimens collected during cancer surgery for detection of bacterial DNA of Helicobacter pylori and viral DNA of High risk HPV (groups A9, A7 and A5/6). Results: Helicobacter pylori DNA was detected in 60 samples (85.7%), while DNA of HPV only in 42 (60%). If focused on HPV-16 as proposed cancer inductor, it was detected in 34 samples (48.5%) only. No DNA of respective agents was detected in 7 samples (10%). There were 21 Helicobacter sole pathogen detections compared with only 3 of HPV. Conclusions: There is no doubt, Helicobacter pylori is a long-term resident in oropharynx and tonsils. This residence most likely influences functions of immune system, so that a newly entering contributor could switch-on the process resulting in cancer development. This could support high incidence of common detection of HPV and Helicobacter pylori in 39 samples (55.7%).

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 6)
DOI 10.11648/j.ajcem.20150306.17
Page(s) 364-367
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Helicobacter pylori, Human Papillomavirus, Real-Time PCR, Cancer, Oropharynx, Genotype

References
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[9] E.N. Dagtekin-Ergür, F. Eren, M.B. Ustün et al., “Investigation of Helicobacter pylori colonization in pharyngeal and palatine tonsil with rapid urease test and immunohistochemical analysis”, Kulak Burun Bogaz Ihtis Derg 2008 18: 85-89.
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[14] P. Lukes, J. Astl, E. Pavlik, B. Potuznikova, I. Sterzl, J. Betka “Helicobacter pylori in tonsillar and adenoid tissue and its possible role in oropharyngeal carcinogenesis”. Folia Biol (Praha) 2008: 54 (2): 33-39.
[15] E. Pavlik, P. Lukes, B. Potuznikova, J. Astl, P. Hrda, A. Soucek, P. Matucha, J. Dosedel, I. Sterzl, “Helicobacter pylori isolated from patients with tonsillar cancer or tonsillitis chronica could be of different genotype compared to isolates from gastrointestinal tract”, Folia Microbiol (Praha), 2007: 52(1): 91- 94.
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[17] D.P. Zandberg, S. Liu, O.G. Goloubeva, L.M. Schumacher and K.J. Cullen, “The rise of HPV positive Oropharyngeal cancer in Black Patients Over Time: University of Maryland 1992-2007” Cancer Prev Res 2015 Jan: 8(1): 12-19.
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  • APA Style

    Emil Pavlik, Eva Nartova, Jaromir Astl, Barbora Drnkova, Petr Lukes, et al. (2015). Detection of Helicobacter pylori and Human Papillomavirus in Peroperative Tissue Biopsies Collected from Malignancies in Oropharyngeal Area. American Journal of Clinical and Experimental Medicine, 3(6), 364-367. https://doi.org/10.11648/j.ajcem.20150306.17

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    ACS Style

    Emil Pavlik; Eva Nartova; Jaromir Astl; Barbora Drnkova; Petr Lukes, et al. Detection of Helicobacter pylori and Human Papillomavirus in Peroperative Tissue Biopsies Collected from Malignancies in Oropharyngeal Area. Am. J. Clin. Exp. Med. 2015, 3(6), 364-367. doi: 10.11648/j.ajcem.20150306.17

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    AMA Style

    Emil Pavlik, Eva Nartova, Jaromir Astl, Barbora Drnkova, Petr Lukes, et al. Detection of Helicobacter pylori and Human Papillomavirus in Peroperative Tissue Biopsies Collected from Malignancies in Oropharyngeal Area. Am J Clin Exp Med. 2015;3(6):364-367. doi: 10.11648/j.ajcem.20150306.17

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  • @article{10.11648/j.ajcem.20150306.17,
      author = {Emil Pavlik and Eva Nartova and Jaromir Astl and Barbora Drnkova and Petr Lukes and Bela Potuznikova and Rami Katra and Jaroslav Kraus and Ivan Sterzl},
      title = {Detection of Helicobacter pylori and Human Papillomavirus in Peroperative Tissue Biopsies Collected from Malignancies in Oropharyngeal Area},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {3},
      number = {6},
      pages = {364-367},
      doi = {10.11648/j.ajcem.20150306.17},
      url = {https://doi.org/10.11648/j.ajcem.20150306.17},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150306.17},
      abstract = {Helicobacter pylori has been reported as pathogen of human GIT. It is associated with type B gastritis and peptic ulcers. Bacterium´s relationship to cancer has also been declared and H. pylori considered cancer-inductor. A number of studies documented H. pylori residence in oropharynx, generating hypotheses on participation in development of cancer in oropharyngeal area. Human papillomaviruses are DNA viruses colonizing skin and mucoid membranes of the host. Their oncogenic potential, especially in genitourinary system, has been confirmed. High-risk type HPV16 (group A9) is frequently reported as cancer-inductor in oropharyngeal area. The aim of this study is to contribute to discussions on induction of malignancies in oropharyngeal area, providing comparison of incidence of one bacterial and one viral pathogen in the cells and tissues of oropharyngeal neoplasia. Using real-time PCR-based tests, we investigated 70 tissue specimens collected during cancer surgery for detection of bacterial DNA of Helicobacter pylori and viral DNA of High risk HPV (groups A9, A7 and A5/6). Results: Helicobacter pylori DNA was detected in 60 samples (85.7%), while DNA of HPV only in 42 (60%). If focused on HPV-16 as proposed cancer inductor, it was detected in 34 samples (48.5%) only. No DNA of respective agents was detected in 7 samples (10%). There were 21 Helicobacter sole pathogen detections compared with only 3 of HPV. Conclusions: There is no doubt, Helicobacter pylori is a long-term resident in oropharynx and tonsils. This residence most likely influences functions of immune system, so that a newly entering contributor could switch-on the process resulting in cancer development. This could support high incidence of common detection of HPV and Helicobacter pylori in 39 samples (55.7%).},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Detection of Helicobacter pylori and Human Papillomavirus in Peroperative Tissue Biopsies Collected from Malignancies in Oropharyngeal Area
    AU  - Emil Pavlik
    AU  - Eva Nartova
    AU  - Jaromir Astl
    AU  - Barbora Drnkova
    AU  - Petr Lukes
    AU  - Bela Potuznikova
    AU  - Rami Katra
    AU  - Jaroslav Kraus
    AU  - Ivan Sterzl
    Y1  - 2015/12/30
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajcem.20150306.17
    DO  - 10.11648/j.ajcem.20150306.17
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 364
    EP  - 367
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20150306.17
    AB  - Helicobacter pylori has been reported as pathogen of human GIT. It is associated with type B gastritis and peptic ulcers. Bacterium´s relationship to cancer has also been declared and H. pylori considered cancer-inductor. A number of studies documented H. pylori residence in oropharynx, generating hypotheses on participation in development of cancer in oropharyngeal area. Human papillomaviruses are DNA viruses colonizing skin and mucoid membranes of the host. Their oncogenic potential, especially in genitourinary system, has been confirmed. High-risk type HPV16 (group A9) is frequently reported as cancer-inductor in oropharyngeal area. The aim of this study is to contribute to discussions on induction of malignancies in oropharyngeal area, providing comparison of incidence of one bacterial and one viral pathogen in the cells and tissues of oropharyngeal neoplasia. Using real-time PCR-based tests, we investigated 70 tissue specimens collected during cancer surgery for detection of bacterial DNA of Helicobacter pylori and viral DNA of High risk HPV (groups A9, A7 and A5/6). Results: Helicobacter pylori DNA was detected in 60 samples (85.7%), while DNA of HPV only in 42 (60%). If focused on HPV-16 as proposed cancer inductor, it was detected in 34 samples (48.5%) only. No DNA of respective agents was detected in 7 samples (10%). There were 21 Helicobacter sole pathogen detections compared with only 3 of HPV. Conclusions: There is no doubt, Helicobacter pylori is a long-term resident in oropharynx and tonsils. This residence most likely influences functions of immune system, so that a newly entering contributor could switch-on the process resulting in cancer development. This could support high incidence of common detection of HPV and Helicobacter pylori in 39 samples (55.7%).
    VL  - 3
    IS  - 6
    ER  - 

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Author Information
  • Department of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

  • Department of Otorhinolaryngology, Third Faculty of Medicine, Charles University in Prague and Faculty Hospital Kralovske Vinohrady, Prague, Czech Republic

  • Department of Otorhinolaryngology, Third Faculty of Medicine, Charles University in Prague and Military Faculty Hospital, Prague, Czech Republic

  • Department of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

  • Department of Otorhinolaryngology, Head and Neck Surgery, First Faculty of Medicine, Charles University in Prague and Motol Faculty Hospital, Prague, Czech Republic

  • Department of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

  • Department of Otolaryngology, Second Faculty of Medicine, Charles University in Prague and Motol Faculty Hospital, Prague, Czech Republic

  • Ear, Nose and Throat Ward, Rudolph and Stephanie Hospital Benesov, Benesov, Czech Republic

  • Institute for Endocrinology, Prague, Czech Republic

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