| Peer-Reviewed

Anti-Inflammatory Effect of Aloe Vera (l) Burm, F. on COX-2 and Histamine Expression in Mice Skin Exposed to UVB

Received: 20 May 2016     Accepted: 28 May 2016     Published: 13 June 2016
Views:       Downloads:
Abstract

The purpose of this study was to investigate the anti-inflammatory effect of topical aloe vera on COX-2 and histamine expression in mice skin exposed to UVB. COX-2 and histamine expression were examined by using immunochemistry staining in skin samples from totally 20 Swiss Albino mice in the control group, UVB group, UVB+Ethanol group, UVB+Aloe group. The study show topical application of aloe vera with a concentration of 200 mg/kg body weight suppress expression of COX-2 in mice skin exposed to UVB, however it have not been proven to reduce histamine expression. Aloe vera with a dose of 200mg/kg body weight has show anti-inflammatory effects by reduction COX-2 expression that assessed 24 hours after the last treatment.

Published in American Journal of Clinical and Experimental Medicine (Volume 4, Issue 4)
DOI 10.11648/j.ajcem.20160404.11
Page(s) 94-97
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Aloe Vera, Anti-inflammatory, COX-2, Histamine, UVB

References
[1] Soter, N. 1995. Sunburn and suntan: Immediate manifestations of photodamage. In: Gilchrist B, (ed.) Photodamage. Blackwell Science, 12-25.
[2] Degruij, F., Vankranen, H. J., Mullenders, L. H. F. 2001. UV-induced DNA damage, mutations and oncogenic pathways in skin cancer. J Photochem Photobiol, 63, 19-27.
[3] Baba, T., Hanada, K., Hashimoto, I. 1996. The study of ultraviolet B-induced apoptosis in cultured mouse keratinocytes and in mouse skin. J Dermat Sci., 12, 18-23.
[4] Haake, A. R., Polakowska, R. R. 1997. Pathways to programmed cell death: Apoptosis and the skin. Curr Opin Dermatol, 4, 247-255.
[5] Black, A. K., Greaves, M. W., Hensby, C. N., Plummer, N. A. 1980. Increased prostaglandins E2 and F2alpha in human skin at 6 and 24h after ultraviolet B irradiation (290–320 nm). Br J Clin Pharmacol, 5, 431–6.
[6] Pentland, A. P., Mahoney, M., Jacobs, S. C., Holtzman, M. J. 1990. Enhanced prostaglandin synthesis after ultraviolet injury is mediated by endogenous histamine stimulation. J Clin Invest, 86, 566-574.
[7] Pentland, A. P. 1998. Arachidonic acid metabolism. In: Freedberg, I. M., Eisen, A. Z., Wolf, K., Austen, K. F., Goldsmith, L. A., Katz, S. I., Fitzpatrick, T. B., (eds.) Fitzpatrick’s Dermatology in General Medicine, 5th edn. New York: McGraw-Hill.
[8] Muller-Decker, K., Scholz, K., Marks, F., Furstenberger, G. 1995 Diferential expression of prostaglandin H synthase isozymes during multistage carcinogenesis in mouse epidermis. Mol Carcinogenesis, 12, 31-41.
[9] Smith, W. L., Garavito, R. M., Dewitt, D. L. 1996. Prostaglandin endoperoxide H synthase (cyclooxygenases-1 and -2). J Biol Chem, 271, 33157-33160.
[10] Tripp, C. S., Blomme, E. A. G., Chinn, K. S., Hardy, M. M., Lacelle, P. & Pentlandz, A. P. 2003. Epidermal COX-2 Induction Following Ultraviolet Irradiation: Suggested Mechanism for the Role of COX-2 Inhibitionin Photoprotection. J Invest Dermatol, 121, 853-61.
[11] Klein, L. M., Lavker, R. M., Matis, W. L. & Murphy, G. F. 1989. Degranulation of human mast cells induces an endothelial antigen central toleukocyteadhesion. Proc. Natl. Acad. Sci, 86, 8972-6.
[12] Sarkar, D., Dutta, A., Das, M., Sarkar, K., Mandal, C. & Chatterjee, M. 2005. Effect of Aloe vera on nitric oxide production by macrophages during inflammation. Indian J Pharmacol 37, 371-5.
[13] Devaraj, A. & Karpagam, T. 2011. Evaluation on anti-inflammatory activity and analgesic effect of aloe vera leaf extract in rats IRJP, 2, 103-10.
[14] Williams C. S., Mann M., Dubois R. N. 1999. The Role of Cyclooxygenase in Inflammation, Cancer, and Development. Oncogene, 18, 7908-7916.
[15] Hamid, A. A. M. A. & Soliman, M. F. 2015. Effect of topical aloe vera on the process of healing of full-thickness skin burn: a histological and immunohistochemical study. Journal of Histology and Histopathology, 2, 1-9.
[16] Benly, P. 2015. Role of Histamine in Acute Inflammation. Pharm. Sci. and Res. 7(6), 373-376
[17] Gilchrest, B. A., Soter, N. A., Stoff, J. S., Mihm, M. C. J. R. 1981. The human sunburn reaction: histologic and biochemical studies. J Am Acad Dermatol, 5, 411–22.
Cite This Article
  • APA Style

    Venyce Laurence Agung, Alwi A. Mappiasse, Siswanto Wahab, Gemini Alam, M. Husni Cangara, et al. (2016). Anti-Inflammatory Effect of Aloe Vera (l) Burm, F. on COX-2 and Histamine Expression in Mice Skin Exposed to UVB. American Journal of Clinical and Experimental Medicine, 4(4), 94-97. https://doi.org/10.11648/j.ajcem.20160404.11

    Copy | Download

    ACS Style

    Venyce Laurence Agung; Alwi A. Mappiasse; Siswanto Wahab; Gemini Alam; M. Husni Cangara, et al. Anti-Inflammatory Effect of Aloe Vera (l) Burm, F. on COX-2 and Histamine Expression in Mice Skin Exposed to UVB. Am. J. Clin. Exp. Med. 2016, 4(4), 94-97. doi: 10.11648/j.ajcem.20160404.11

    Copy | Download

    AMA Style

    Venyce Laurence Agung, Alwi A. Mappiasse, Siswanto Wahab, Gemini Alam, M. Husni Cangara, et al. Anti-Inflammatory Effect of Aloe Vera (l) Burm, F. on COX-2 and Histamine Expression in Mice Skin Exposed to UVB. Am J Clin Exp Med. 2016;4(4):94-97. doi: 10.11648/j.ajcem.20160404.11

    Copy | Download

  • @article{10.11648/j.ajcem.20160404.11,
      author = {Venyce Laurence Agung and Alwi A. Mappiasse and Siswanto Wahab and Gemini Alam and M. Husni Cangara and Ilhamjaya Patellongi},
      title = {Anti-Inflammatory Effect of Aloe Vera (l) Burm, F. on COX-2 and Histamine Expression in Mice Skin Exposed to UVB},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {4},
      number = {4},
      pages = {94-97},
      doi = {10.11648/j.ajcem.20160404.11},
      url = {https://doi.org/10.11648/j.ajcem.20160404.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20160404.11},
      abstract = {The purpose of this study was to investigate the anti-inflammatory effect of topical aloe vera on COX-2 and histamine expression in mice skin exposed to UVB. COX-2 and histamine expression were examined by using immunochemistry staining in skin samples from totally 20 Swiss Albino mice in the control group, UVB group, UVB+Ethanol group, UVB+Aloe group. The study show topical application of aloe vera with a concentration of 200 mg/kg body weight suppress expression of COX-2 in mice skin exposed to UVB, however it have not been proven to reduce histamine expression. Aloe vera with a dose of 200mg/kg body weight has show anti-inflammatory effects by reduction COX-2 expression that assessed 24 hours after the last treatment.},
     year = {2016}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Anti-Inflammatory Effect of Aloe Vera (l) Burm, F. on COX-2 and Histamine Expression in Mice Skin Exposed to UVB
    AU  - Venyce Laurence Agung
    AU  - Alwi A. Mappiasse
    AU  - Siswanto Wahab
    AU  - Gemini Alam
    AU  - M. Husni Cangara
    AU  - Ilhamjaya Patellongi
    Y1  - 2016/06/13
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajcem.20160404.11
    DO  - 10.11648/j.ajcem.20160404.11
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 94
    EP  - 97
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20160404.11
    AB  - The purpose of this study was to investigate the anti-inflammatory effect of topical aloe vera on COX-2 and histamine expression in mice skin exposed to UVB. COX-2 and histamine expression were examined by using immunochemistry staining in skin samples from totally 20 Swiss Albino mice in the control group, UVB group, UVB+Ethanol group, UVB+Aloe group. The study show topical application of aloe vera with a concentration of 200 mg/kg body weight suppress expression of COX-2 in mice skin exposed to UVB, however it have not been proven to reduce histamine expression. Aloe vera with a dose of 200mg/kg body weight has show anti-inflammatory effects by reduction COX-2 expression that assessed 24 hours after the last treatment.
    VL  - 4
    IS  - 4
    ER  - 

    Copy | Download

Author Information
  • Dermatovenereology Department, Medical Faculty, Hasanuddin University, Makassar, Indonesia

  • Dermatovenereology Department, Medical Faculty, Hasanuddin University, Makassar, Indonesia

  • Dermatovenereology Department, Medical Faculty, Hasanuddin University, Makassar, Indonesia

  • Pharmacy Faculty, Hasanuddin University, Makassar, Indonesia

  • Pathological and Anatomy Department, Medical Faculty, Hasanuddin University, Makassar, Indonesia

  • Biostatistic, Medical Faculty, Hasanuddin University, Makassar, Indonesia

  • Sections