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Research Article
Isolation and Probiotic Characterization of Lactic Acid Bacteria from Kindirmou and Pendidam in Adamawa Region (Cameroon)
Issue:
Volume 12, Issue 2, June 2024
Pages:
35-48
Received:
8 March 2024
Accepted:
20 March 2024
Published:
12 April 2024
Abstract: The microorganisms intended for use as probiotics in food formulation should exert health benefit effects and be regarded as safe for animals and humans uses. The aim of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from pendidam and kindirmou, two traditional fermented milks (TFM) produced in the Adamawa region (Cameroon). Twenty-five samples (pendidam: 13 and kindirmou: 12) were randomly collected in five markets of Ngaoundere (n = 17 samples) and Meiganga (n = 8 samples). These samples were screened for their antimicrobial activity, and nine TFMs were retained. Lactic acid bacteria were isolated from these samples and their antimicrobial activity was already evaluated. Based on the inhibition zone, twenty-two LABs were retained and examined in vitro for potential probiotic properties based on their low pH tolerance, resistance to bile salts, tolerance to simulated gastrointestinal juices, hydrophobicity, autoaggregation, gelatinase and hemolytic activities. The outcome of these parameters studied was used as input data for a principal component analysis (PCA) to select the most promising isolate, and the six potential probiotic isolates were characterized through a biochemical profile. The characterized isolates have been identified as Lactiplantibacillus plantarum, Lacticaseibacillus casei, and Lactococcus lactis. Traditional fermented milks contain LAB with important properties that can be utilized in the formulation of functional foods.
Abstract: The microorganisms intended for use as probiotics in food formulation should exert health benefit effects and be regarded as safe for animals and humans uses. The aim of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from pendidam and kindirmou, two traditional fermented milks (TFM) produced in the Adamawa...
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Research Article
Effects of the Drying Technique Used on the Contents of Total Polyphenols, Flavonoids and Antioxidant Activity of Five Varieties of Mangoes (Mangifera Indica) Dried in Senegal
Ibrahima Ba*
,
Mouhamadou Fofana,
El Hadji Gorgui Diouf,
Bédié Mbow,
Nicolas Cyrille Ayessou,
Fatou Dieng Faye
Issue:
Volume 12, Issue 2, June 2024
Pages:
49-59
Received:
8 April 2024
Accepted:
3 May 2024
Published:
30 May 2024
DOI:
10.11648/j.ab.20241202.12
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Abstract: The Ziguinchor region (Kaguitte village), where the mango samples were collected, is an area that is very affected by post-school losses of seasonal fruit in general. Faced with this problem, the women in the GIE (economic interest group) have been processing mangoes into by-products for several years, particularly drying, which is one of the mwain techniques for preserving agricultural and food products. It is a process that is used in the production of many food products. To carry out this drying, three techniques were used: the one used by the women of the locality (Normal) and two others with osmotic dehydration for two hours and three hours respectively. After peeling, it was found that the improved varieties Knt and Kt had the highest pulp yields (60% of their total mass) and that the Sl variety had the lowest yield. In this work, we followed the variation of polyphenol contents and antioxidant powers according to the technique and the mango variety. For total polyphenols, the Folin-Ciocalteu method was used and antioxidant activity was assessed with 2,2-diphenyl-1-pycrilhydrazyl (DPPH°). The results showed that the antioxidant power, significantly decreases after treatment of the four varieties (example: Knt normal-AAR=0.83%, Treatment-2h-AAR=0.69% and Treatment-3h-AAR=0.59%) with the exception of Dr variety (Normal-AAR=0.68% and Treatment-2h-AAR=0.79% and Treatment-3h-AAR=0.78%). The determination of total polyphenols in these samples showed a much more obvious variation between varieties and less important after the treatment. Thus we have: Knt (Normal-0.07g/100g, treatment-2h-0.03g/100g and treatment-3h-0.19g/100g), Kt (Normal-0.27g/100g, treatment-2h-0.07g/100g and treatment-3h-0.06g/100g) and Sl (Normal-0.11g/100g, treatment-2h-0.07g/100g and treatment-3h-0.21g/100g) Analyses (of Variance and linear models) show that the varieties Knt (treatment-2h-0.0087g/100g and normal-0.0033g/100g), Dr (treatment-2h-0.0023g/100g and normal-0.0019g/100g) and Sl (treatment-2h-0.0023g/100g and normal-0.0017g/100g), respectively, have quite high flavonoid contents. These experimental results show that the technique used (Normal) by the local units preserves the antioxidant compounds well and in some cases it is necessary to treat the different varieties before drying them.
Abstract: The Ziguinchor region (Kaguitte village), where the mango samples were collected, is an area that is very affected by post-school losses of seasonal fruit in general. Faced with this problem, the women in the GIE (economic interest group) have been processing mangoes into by-products for several years, particularly drying, which is one of the mwain...
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Research Article
Mineral Content, Antioxidant Properties in vitro, Reduction of Inflammation, and Liver Steatosis in vivo by Ngaoundal Propolis in Wistar Rats Fed an Atherogenic Diet
Tsague Marthe Valentine*,
Metchi Donfack Mireille Flaure,
Nodem Sohanang Francky Steve,
Kingha Tekombo Bernice Mireille,
Ondo Eyi Ned Merlyne Larissa,
Hassimi Moussa,
Dang’ne Madoue Denis,
M’bann Nsonngan Salomon,
Ahamat Abakar,
Tchuenguem-Fohouo Fernand Nestor,
Ze Minkande Jacqueline
Issue:
Volume 12, Issue 2, June 2024
Pages:
60-75
Received:
14 April 2024
Accepted:
7 May 2024
Published:
30 May 2024
Abstract: Several studies have reported the benefits of Propolis in the treatment of various disorders such as parasitic infections, bacterial infections, wounds, and burns. The overall aim of this study was to evaluate the preventive effects and anti-inflammatory activities of the hydroethanolic extract (EthP) and the fraction powder ≤ 125 µm of Propolis (PP) on atherogenic diet-induced non-alcoholic fatty liver disease. Dry Propolis was finely ground, a first part was macerated in a mixture (30:70 v/v water and ethanol) and a second part was fractionated by sieving with a sieve mesh (≤125 µm). The powder fraction≤ 125µm (PP) and Propolis hydroethanolic extract (EthP) obtained were used to characterize the mineral composition in vitro and in vivo antioxidant and anti-inflammatory properties. 20 male Wistar rats were divided into 5 groups EthP and PP were administered orally to the rats at the same dose (250 mg/kg bw) and fed simultaneously with an atherogenic diet for 45 days. At the end of the experiment, the lipid profile, transaminase aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in serum, and antioxidants were measured at the organ level (aorta, liver, kidney, and heart). The activities of all parameters were significant (p < 0.05). The results of this study show that Propolis had a significantly (p<0.0001) lower in vitro mineral composition in Iron by 32.56%; in Zinc by 83.21%; in Calcium by 10.82% and in Manganese by 21.40% at the PP level compared to EthP. Antioxidant capacity (DPPH, TAC, and FRAP), which increased with Propolis concentration. High polyphenol content (EthP>PP). Treatment with EthP250 and PP250 significantly (p<0.05) reduced serum ALT by 34.27% and 47.36%, creatinine by 67.36% and 37.5%, TG by 63.91% and by 20.18%, IL-17 expression by 50.25% and 100% respectively. HDL-c levels were significantly increased by 47.7% (p<0.001) in serum compared with TN. NO levels increased significantly (p<0.001) by 1.38% and 1.63% in the aorta respectively. MDA levels were significantly reduced by 55.12% (p<0.0001) and 76.09% (p<0.05) in the liver respectively. This study demonstrated the efficacy of Propolis in the management of non-alcoholic hepatic steatosis and its anti-inflammatory capacity.
Abstract: Several studies have reported the benefits of Propolis in the treatment of various disorders such as parasitic infections, bacterial infections, wounds, and burns. The overall aim of this study was to evaluate the preventive effects and anti-inflammatory activities of the hydroethanolic extract (EthP) and the fraction powder ≤ 125 µm of Propolis (P...
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Research Article
Performances of Proteinuria as Compared with Albuminuria in Screening for Microalbuminuria During Sickle Cell Anaemia
El Hadji Malick Ndour*,
Rokhaya Dione,
Fatou Gueye-Tall,
Sokhna Mara,
Indou Deme-Ly,
Moussa Seck,
Aliou Alioune Ndongo,
Moustapha Djite,
Helene Ange Therese Sagna-Bassene,
Nene Oumou Kesso Barry,
Pape Matar Kandji,
Coumba Kamby,
El Hadji Ousmane Sene,
Papa Madieye Gueye,
Ibrahima Diagne,
Saliou Diop,
Philomene Lopez-Sall,
Aynina Cisse
Issue:
Volume 12, Issue 2, June 2024
Pages:
76-84
Received:
14 May 2024
Accepted:
30 May 2024
Published:
13 June 2024
Abstract: Albuminuria is the gold standard for the screening of microalbuminuria, a biomarker of early onset of nephropathy during sickle cell anemia (SCA). Nephropathy increase morbidity and mortality of SCA in the absence of appropriate treatment. However, albuminuria is not readily available or affordable in resource-limited countries, so in 2012 Kidney Diseases Improving Global Outcomes (KDIGO) proposed using proteinuria at a threshold of 150 mg/g urine creatinine to screen for microalbuminuria in these settings. The aim of this study was therefore to assess the performance of proteinuria in screening microalbuminuria in sub-Saharan Senegalese sickle cell patients. Albuminuria in recruited SS sickle cell patients was expressed as a urine albumin-to-creatinine ratio (UACR) and proteinuria as a urine proteins-to-creatinine ratio (UPCR). The prevalence of microalbuminuria, Cohen's kappa coefficient and areas under the curve (AUC) were then determined to assess the performance of proteinuria in detecting microalbuminuria. A total of 150 patients with a median age of 20 years [minimum-maximum: 4-57] and a female proportion of 51.33% were included in the study. Microalbuminuria was present in 42.38% (n=64) of subjects according to the UPCR. The Cohen's kappa coefficient was 0.41 [IC95%: 0.27-0.56] and the AUC 0.71 [IC95%: 0.64 - 0.81] with UPCR 150mg/g. The best Cohen's kappa coefficient and AUC were observed with an UPCR threshold of 135 mg/g. Our results confirm that proteinuria is useful in screening for microalbuminuria and show that RPCU 135 mg/g would be the optimal cut-off for detecting microalbuminuria in Senegalese sickle cell anemia patients.
Abstract: Albuminuria is the gold standard for the screening of microalbuminuria, a biomarker of early onset of nephropathy during sickle cell anemia (SCA). Nephropathy increase morbidity and mortality of SCA in the absence of appropriate treatment. However, albuminuria is not readily available or affordable in resource-limited countries, so in 2012 Kidney D...
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Research Article
Association Between Serum 25(OH)D Concentration and Clinical and Biological Complications in Pediatric Patients with Sickle Cell Disease
Fatou Gueye Tall*,
Pape Matar Kandji,
El Hadji Malick Ndour,
Serigne Saliou Mbancké,
Moustapha Djité,
Oumou Kesso Barry,
Aynina Cissé,
Philoméne Lopez Sall,
Papa Madièye Gueye
Issue:
Volume 12, Issue 2, June 2024
Pages:
85-91
Received:
25 April 2024
Accepted:
22 May 2024
Published:
19 June 2024
DOI:
10.11648/j.ab.20241202.15
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Abstract: In addition to its role in phosphocalcic and bone metabolism, vitamin D also plays a global role in health. Despite high levels of sunshine, vitamin D deficiency remains a public health problem in sub-Saharan Africa, where sickle cell disease predominates. This study aimed to investigate an association between serum vitamin D (25(OH)D) levels and the occurrence of clinical and biological complications. The study was carried out in children with SS sickle cell disease aged 1 to 17 years (75 patients) matched to 17 AA controls. Plasma 25(OH)D concentration was obtained by immunoassay. The clinical complications studied were caso-occlusive crisis, osteomyelitis, osteonecrosis, acute chest syndrome, and priapism. Biological parameters included blood count, ionized calcium, and phosphorus. Statistical analysis was performed using R Studio 4.1.2 software. The significance threshold was 5%. Our study revealed a high prevalence of vaso-occlusive crisis (97%). Vitamin D deficiency was found in 4% of patients (3 SS patients) and 36% (27 SS patients) had a plasma concentration between 10 and 30 ng/ml. The association study revealed a negative association between vitamin D and the number of vaso-occlusive crises (r = -0.51; p < 0.001). We noted a positive association between vitamin D and blood calcium (r = 0.347; p < 0.002), phosphatemia (r = 0.347; p < 0.002), and hemoglobin (r = 0.243; p < 0.035). Vitamin D is correlated with certain clinical and biological complications. Vitamin D supplementation in children with sickle cell disease (SS) would therefore be relevant for better management of this disease.
Abstract: In addition to its role in phosphocalcic and bone metabolism, vitamin D also plays a global role in health. Despite high levels of sunshine, vitamin D deficiency remains a public health problem in sub-Saharan Africa, where sickle cell disease predominates. This study aimed to investigate an association between serum vitamin D (25(OH)D) levels and t...
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