Advances in Biochemistry

Volume 1, Issue 5, December 2013

  • In Vitro Processing of Glutamyl Endopeptidase Proenzymes from Enterococcus faecalis and Importance of N-terminal Residue in Enzyme Catalysis

    Shakh M. A. Rouf, Y. Ohara-Nemoto, T. Ono, Y. Shimoyama, S. Kimura, T. K. Nemoto

    Issue: Volume 1, Issue 5, December 2013
    Pages: 73-80
    Received: 22 November 2013
    Published: 30 December 2013
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    Abstract: Glutamyl endopeptidase from Enterococcus faecalis, designated SprE, is one of the important virulence factors secreted as zymogen. In the present study we expressed recombinant SprE proenzyme (pro-SprE) in Escherichia coli and investigated the in vitro processing to mature SprE. It was found that trypsin could efficiently produce the active form of... Show More
  • Endo-N-Acetyl-β-D-Glucosaminidases and Peptide-N4-(N-acetyl-β-D-Glucosaminyl) Asparagine Amidases: More Than Just Tools

    Yannis Karamanos

    Issue: Volume 1, Issue 5, December 2013
    Pages: 81-99
    Received: 13 December 2013
    Published: 30 December 2013
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    Abstract: Since the discovery of endo-N-acetyl-β-D-glucosaminidases (ENGase) and peptide-N4-(N-acetyl-β-D-glucosaminyl) asparagine amidases (PNGase) most of the published work described their use for structural studies. Less attention was given to the potential roles of those enzymes in the physiology of the cells/organisms they produced them. The scope of t... Show More