Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis
Dalia A. Shaheen,
Hisham M. Habib
Issue:
Volume 2, Issue 2, April 2014
Pages:
29-33
Received:
13 March 2014
Accepted:
9 April 2014
Published:
20 April 2014
Abstract: Background: The multiple pro-inflammatory cascades described in RA lead to persistent synovitis, resulting in articular cartilage and bone damages. The up-regulation of interferon-inducible genes has been found in the synovial lining regions and may play an important role in the pathogenesis of RA. Objective: The aim of the current study is to examine the expression of IL-29 and its receptors IL-28Rα in blood and synovial fluid (SF) in RA patients and investigate the correlation of IL-29 with disease activity and other laboratory characters of RA. Methods: The study was carried out on 108 RA patients, 70 OA patients and 80 healthy control subjects. Clinical assessment of disease activity in RA patients and disease activity score with DAS28 had been done. IL29 was detected in the serum and synovial fluid of all samples by ELISA. Real time PCR was used for human IL-29 and IL-28Rα expression from PBMC. Results: expression of IL-29 mRNA was significantly higher in RA PBMCs when compared to OA and HC (132±13.8, 49± 11.2, 33.3± 10.5; respectively P <0.05). Similarly, IL-28Rα mRNA expression was significantly higher in RA PBMCs when compared to OA and HC (30.5±9.7, 13.3±4.5, 8.5± 3.3; respectively P <0.05). The mean level of IL-29 in SF was increased in RA and found also much higher in RA (17.29 ± 10.19pg/ml) than OA (8.33 ±3.89 pg/ml) (P = 0.01). There is a significant correlation between serum levels of circulating IL- 29 and DAS28, CRP, ESR, RF, and anti-CCP. Conclusion: our data have presented new evidence that IL-29 may contribute to RA pathogenesis
Abstract: Background: The multiple pro-inflammatory cascades described in RA lead to persistent synovitis, resulting in articular cartilage and bone damages. The up-regulation of interferon-inducible genes has been found in the synovial lining regions and may play an important role in the pathogenesis of RA. Objective: The aim of the current study is to exam...
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The Effect of Serum Free Testosterone Level on Glycemic Control and Atherosclerosis in Type 2 Diabetic Men
Dalia A. Shaheen,
Doaa A. Shahin,
Alaa Aldeen Habib,
Amani M. El Amin Ali
Issue:
Volume 2, Issue 2, April 2014
Pages:
34-39
Received:
10 May 2014
Accepted:
29 May 2014
Published:
10 June 2014
Abstract: Background: Atherosclerosis is a complex disease of the arteries characterized by endothelial dysfunction, vascular inflammation, and the build-up of lipids within the intima of the vessel wall. Testosterone has a central or permissive role in pathogenesis of the metabolic syndrome and type 2 diabetes. Insulin resistance is associated with several CVD risk factors such as obesity, dyslipidaemia, hypertension and the proinflammatory state. We aim to disclose the relationship between serum testosterone concentration and carotid atherosclerosis and its risk factors in men with type 2 diabetes. Patients and methods: The study population comprised 123 consecutive men of Type 2 diabetes. Retinopathy and nephropathy were ranked and graded respectively. Cardiovascular disease was defined as the presence of previous myocardial infarction or cerebral infarction. Total cholesterol and triglyceride concentrations were determined and hemoglobin Alc was measured. Assessment for the presence of carotid atherosclerosis was done, using ultrasonographic measurement of carotid intima media thickness (IMT). The relationship between serum testosterone concentration and carotid intima-media thickness IMT was investigated in all patients. Results: The mean of IMT for all patients was 0.96 ± 0.28 mm. Mean IMT was significantly greater in patients with lower concentrations of F-tes than in patients with higher concentrations of F-tes. (P= 0.038). Relationship between serum free testosterone concentration and other variables showed a negative correlation with patients’ age, patients’ age at onset, duration of diabetes, BMI, HbA1c, systolic and diastolic blood pressure, and total cholesterol concentrations and mean IMT in men with type 2 diabetes. No significant correlation was found between F-tes with triglyceride and negative correlation with mean IMT. Conclusion: Serum free testosterone concentration was found to be low in type 2 diabetic men. It has a negative correlation with patients’ age, patients’ age at onset of the disease, duration of diabetes, BMI and HbA1c, total cholesterol concentrations systolic and diastolic blood pressure and mean IMT. This may disclose the different mechanisms played by testosterone in the pathogenesis of cardiovascular risk in men with type 2 diabetes.
Abstract: Background: Atherosclerosis is a complex disease of the arteries characterized by endothelial dysfunction, vascular inflammation, and the build-up of lipids within the intima of the vessel wall. Testosterone has a central or permissive role in pathogenesis of the metabolic syndrome and type 2 diabetes. Insulin resistance is associated with several ...
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