Research Article
Intracellular Adaptation of Listeria monocytogenes and Listeria ivanovii Isolated from Fresh Produce in Côte d’Ivoire: An in Vitro Infection Study Using JEG3 Human Cells
Issue:
Volume 10, Issue 3, September 2025
Pages:
57-65
Received:
1 July 2025
Accepted:
14 July 2025
Published:
30 July 2025
DOI:
10.11648/j.ijfsb.20251003.11
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Abstract: Listeria monocytogenes (L. monocytogenes) is a pathogen that frequently contaminates foods, and is the cause of listeriosis worldwide. Although L. monocytogenes is the primary species associated with human listeriosis, Listeria ivanovii (L. ivanovii) traditionally considered pathogenic mainly to ruminants has also been implicated in rare but severe infections in immunocompromised humans. The objectives of this study were to examine the ability of L. monocytogenes and L. ivanovii strains isolated from fresh vegetables and market garden produce in Abidjan, Côte d'Ivoire, to enter and multiply in JEG3 cells, to disseminate and to form LisCVs persistence vacuoles. After identification by 16sDNAr gene sequencing and serogrouping by PCR of Listeria strains isolated from fresh vegetables and market garden produce, three strains were identified as belonging to the species L. monocytogenes (2 strains, L208 and L238) and L. ivanovii (1 strain, L135). In vitro infection was carried out using JEG3 trophoblastic cells, with three reference strains (L. monocytogenes EGDe serotype 1/2a, Listeria monocytogenes CLIP80459 serotype 4b and L. ivanovii ATCC19119) serving as controls. The results showed that all the Listeria strains tested had similar characteristics in terms of their ability to penetrate, multiply and form LisCV, and higher cytotoxicity in L. monocytogenes species than in L. ivanovii in general. L. monocytogenes strains L208 and L238 showed similar invasion rates to L. monocytogenes CLIP80459.
Abstract: Listeria monocytogenes (L. monocytogenes) is a pathogen that frequently contaminates foods, and is the cause of listeriosis worldwide. Although L. monocytogenes is the primary species associated with human listeriosis, Listeria ivanovii (L. ivanovii) traditionally considered pathogenic mainly to ruminants has also been implicated in rare but severe...
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Pekoula Senaho Fernand*
