Abstract: The conditions of processing, distribution and sale of beef expose it to various contaminations. These contaminations can be potentially pathogenic microorganisms that would in this case represent a risk to the health of consumers. This study aims to identify potentially pathogenic strains of Escherichia coli and Staphylococcus aureus present in fresh and braised beef, as well as to determine their phenotypic antibiotic resistance profiles. Thus, 300 samples, including 100 samples of fresh beef from the slaughterhouse, 100 samples of fresh beef from the market and 100 samples of braised beef or "choukouya", were collected in Abidjan. Escherichia coli and Staphylococcus aureus were isolated and identified using biochemical and molecular methods. Microbiological analyses revealed that 100% of the fresh beef samples from the slaughterhouse and market were contaminated with E. coli and S. aureus. Braised beef had a contamination rate of 6% (E. coli) and 30% (S. aureus). A total of 144 (92.9%) out of 155 Escherichia coli strains and 76 (57.6%) out of 132 Staphylococcus strains were confirmed as Escherichia coli and Staphylococcus aureus, respectively, after molecular identification. The prevalences of E. coli and S. aureus in slaughterhouse meat were 92.5% and 84.2%, respectively. They were 93.8% and 59.4% in fresh meat and 100% and 38.6% in braised beef. The susceptibility of the strains to antibiotics was assessed by the agar diffusion method. Strains isolated from fresh slaughterhouse and market meats were more resistant to the antibiotics tested. Only one strain (0.7%) producing extended-spectrum beta-lactamases (ESBL) was detected in Escherichia coli, and no methicillin resistance was observed in Staphylococcus aureus.
Abstract: The conditions of processing, distribution and sale of beef expose it to various contaminations. These contaminations can be potentially pathogenic microorganisms that would in this case represent a risk to the health of consumers. This study aims to identify potentially pathogenic strains of Escherichia coli and Staphylococcus aureus present in fr...Show More
Abstract: Rhizobia are diazotrophic bacteria that fix nitrogen after becoming established inside the root nodules of legumes (Fabaceae). This study was conducted to isolate, characterize and evaluate the symbiotic effectiveness of rhizobia nodulating common bean. Nodule sample were collected from six randomly selected kebele of the study area for nodule induction under controlled condition. At the flowering stage after 45 days of plant growth, root nodules were collected and rhizobia bacteria isolated. The isolates were allowed for different biochemical and physiological characterization. Presumptive tests confirmed that isolates were root nodulating rhizobia. Rhizobial inoculants (Bio-fertilizer) effect on the host crop was tested under greenhouse condition on sterilized river sand culture. Plant treated with KNO3 were set as positive and plants treated with sterile distilled water were used as negative control. Isolates treated plant group were experimental group. The experiment was laid in CRD design. After 45 days of inoculation different growth, related parameters were investigated. Collected data on growth related parameter after rhizobia inoculation were analyzed using SAS software version 9.1. Rhizobial treatment effect on plant growth related parameters were investigated. Effect of treatment was significant at (P<0.05) difference on SDW, NDW, SFW. SE. rhizobial treatment on host crop shown significant effect and rhizobia were capable to infect the host crop in sterile river sand culture under greenhouse condition. The isolates were highly effective on their symbiotic effectiveness rate. Therefore, the molecular characterization of the isolates of the area and effect of inoculant on yield related parameter of host crop (Phaseolus Vulgaris L.) study on field trial should be future perspective of the area.
Abstract: Rhizobia are diazotrophic bacteria that fix nitrogen after becoming established inside the root nodules of legumes (Fabaceae). This study was conducted to isolate, characterize and evaluate the symbiotic effectiveness of rhizobia nodulating common bean. Nodule sample were collected from six randomly selected kebele of the study area for nodule indu...Show More