International Journal of Microbiology and Biotechnology

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Antimicrobial Effect of Moringa oleifera Leaves Extract on Foodborne Pathogens in Ground Beef

Received: 8 January 2024    Accepted: 26 January 2024    Published: 5 February 2024
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Abstract

The study was done to examine the microbiological effects of Moringa oleifera as a preservation agent on ground beef held at 4°C for 72 hours. The study compared Moringa oleifera concentrations of 0.5%, 1.0%, 1.5%, and 2.0% to a preservative-free control group. This study measured Total bacteria counts (TBC), Total coliform count (TCC), Staphylococcus, Salmonella and E. coli. The study found a dose-dependent relationship between Moringa oleifera dosage and microbial populations. All Moringa oleifera concentrations demonstrated lower total bacterial counts (TBC) than the control group. The highest concentration (2.0%) showed the greatest reduction. This suggests Moringa oleifera could prevent ground beef bacterial growth. In TCC, Moringa oleifera reduced coliform bacteria better at higher concentrations. The concentrations of 1.5% and 2.0% reduced coliform counts significantly compared to the control group, demonstrating their efficacy in regulating them. Moringa oleifera showed dose-dependent antibacterial activity against Staphylococcus and E. coli. Increased preservative doses significantly reduced Staphylococcus and E. coli counts, suggesting they can improve food safety by reducing harmful microorganisms. Moringa oleifera appears to be an effective natural preservative, extending the shelf life and microbiological properties of ground beef under refrigerated storage. This study suggests employing natural preservatives to improve food safety and quality, which is important for the meat business.

DOI 10.11648/ijmb.20240901.13
Published in International Journal of Microbiology and Biotechnology (Volume 9, Issue 1, April 2024)
Page(s) 15-20
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Moringa oleifera, Ground Beef, Flavonoid Content, Foodborne Pathogens

References
[1] Abdull Razis, A. F., Ibrahim, M. D., & Kntayya, S. B. (2014). Health benefits of Moringa oleifera. Asian Pacific Journal of Cancer Prevention, 15 (20), 8571–8576.
[2] Adeyemi, K. D., El-Imam, A. A., Dosunmu, O. O., & Lawal, O. K. (2013). Effect of Moringa oleifera marinade on microbial stability of smoke-dried African catfish (Clarias gariepinus). Ethiopian Journal of Environmental Studies and Management, 6(1), 104-109.
[3] Behbahani, B. A., Noshad, M., & Jooyandeh, H. (2020). Improving oxidative and microbial stability of beef using Shahri Balangu seed mucilage loaded with Cumin essential oil as a bioactive edible coating. Biocatalysis and Agricultural Biotechnology, 24, 101563.
[4] Dadi, D. W., Emire, S. A., Hagos, A. D., & Assamo, F. T. (2018). Influences of different drying methods and extraction solvents on total phenolic and flavonoids, and antioxidant capacity of Moringa stenopetala leaves. Journal of Pharmacognosy and Phytochemistry, 7(1), 962-967.
[5] Ehuwa, O., Jaiswal, A. K., & Jaiswal, S. (2021). Salmonella, food safety and food handling practices. Foods, 10(5), 907.
[6] Elhadi, D. A.; Elgasim, E. A.; Mohamed Ahmed, I. A. Microbial and oxidation characteristics of refrigerated chicken patty incorporated with moringa (Moringa oleifera) leaf powder. CyTA J. Food 2017, 15, 234–240.
[7] Falowo, A. B., Fayemi, P. O., & Muchenje, V. (2014). Natural antioxidants against lipid–protein oxidative deterioration in meat and meat products: A review. Food Research International, 64, 171–181.
[8] Falowo, A. B.; Muchenje, V.; Hugo, C. J.; Charimba, G. In vitro antimicrobial activities of Bidens pilosa and Moringa oleifera leaf extracts and their effects on ground beef quality during cold storage. Cyta J. Food 2016, 14, 541–546.
[9] Harrigan, W. F. (1998). Laboratory methods in food microbiology. Gulf professional publishing.
[10] ISO 16649-2: 2001 (2001) Microbiology of food and animal feedingstuffs—Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli—colony count technique at 44°C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide.
[11] Jayawardana, B. C.; Liyanage, R.; Lalantha, N.; Iddamalgoda, S.; Weththasinghe, P. Antioxidant and antimicrobial activity of drumstick (Moringa oleifera) leaves in herbal chicken sausages. LWT Food Sci. Technol. 2015, 64, 1204–1208.
[12] Kim, S., Cho, A. R., & Han, J. (2013). Antioxidant and antimicrobial activities of leafy green vegetable extracts and their applications to meat product preservation. Food Control, 29, 112–120.
[13] Martin, N. H., Trmčić, A., Hsieh, T. H., Boor, K. J., & Wiedmann, M. (2016). The evolving role of coliforms as indicators of unhygienic processing conditions in dairy foods. Frontiers in microbiology, 7, 1549.
[14] Mashau, M. E.; Munandi, M.; Ramashia, S. E. Exploring the inflfluence of Moringa oleifera leaves extract on the nutritional properties and shelf life of mutton patties during refrigerated storage. CyTA J. Food 2021, 19, 389–398.
[15] Moyo, B.; Masika, P. J.; Muchenje, V. Antimicrobial activities of Moringa oleifera Lam leaf extracts. Afr. J. Biotechnol. 2012, 11, 2797–2802.
[16] Muthukumar, M., Naveena, B. M., Vaithiyanathan, S., Sen, A. R., & Sureshkumar, K. (2014). Effect of incorporation of Moringa oleifera leaves extract on quality of ground pork patties. Journal of Food Science and Technology, 51(11), 3172–3180.
[17] Pal, M., Merera, O., Abera, F., Rahman, M. T., & Hazarika, R. A. (2015). Salmonellosis: A major foodborne disease of global significance. Beverage Food World, 42(12), 21-24.
[18] Pop, O. L., Kerezsi, A. D., & Ciont, C. (2022). A Comprehensive Review of Moringa oleifera Bioactive Compounds—Cytotoxicity Evaluation and Their Encapsulation. Foods, 11(23), 3787.
[19] Rahman, M. H.; Alam, M. S.; Monir, M. M.; Rahman, S. M. E. Effect of Moringa oleifera leaf extract and synthetic antioxidant on quality and shelf-life of goat meat nuggets at frozen storage. Int. J. Food Res. 2020, 7, 34–45.
[20] Rocchetti, G., Pagnossa, J. P., Blasi, F., Cossignani, L., Piccoli, R. H., Zengin, G.,... & Lucini, L. (2020). Phenolic profiling and in vitro bioactivity of Moringa oleifera leaves as affected by different extraction solvents. Food Research International, 127, 108712.
[21] Shah, M. A., Don Bosco, S. J., & Mir, S. A. (2015). Effect of Moringa oleifera leaf extract on the physicochemical properties of modified atmosphere packaged raw beef. Food Packaging and Shelf Life, 3, 31–38.
[22] Shan, B., Cai, Y.-Z., Brooks, D. B. & Corke, H. (2009). Antibacterial and antioxidant effects of five spice and herb extracts as natural preservatives of raw pork. Journal of the Science of Food and Agriculture 89, 1879-1885.
[23] Soladoye, O. P., Juárez, M. L., Aalhus, J. L., Shand, P., & Estévez, M. (2015). Protein oxidation in processed meat: Mechanisms and potential implications on human health. Comprehensive Review in Food Science and Food Safety, 14(2), 106–122.
[24] Vongsak, B., Sithisarn, P., & Gritsanapan, W. (2013). Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions. Industrial Crops and Products, 49, 419-421.
[25] Vongsak, B., Sithisarn, P., Mangmool, S., Thongpraditchote, S., Wongkrajang, Y., & Gritsanapan, W. (2013). Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Industrial crops and products, 44, 566-571.
[26] Zhang, H., Peng, X., Li, X., Wu, J., & Guo, X. (2017). The application of clove extract protects Chinese-style sausages against oxidation and quality deterioration. Korean Journal for Food Science of Animal Resources, 37(1), 114–122.
[27] Zullaikah, S., Naulina, R. Y., Meinawati, P., Fauziyah, K., Rachimoellah, M., Rachmaniah, O.,... & Prasetyo, E. N. (2019, June). Enhanced Extraction of phenolic compounds from Moringa oleifera leaves using subcritical water ethanol mixture. In IOP Conference Series: Materials Science and Engineering (Vol. 543, No. 1, p. 012021). IOP Publishing.
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  • APA Style

    Mwankunda, L. J., Nyamete, F., Kilima, B. (2024). Antimicrobial Effect of Moringa oleifera Leaves Extract on Foodborne Pathogens in Ground Beef. International Journal of Microbiology and Biotechnology, 9(1), 15-20. https://doi.org/10.11648/ijmb.20240901.13

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    ACS Style

    Mwankunda, L. J.; Nyamete, F.; Kilima, B. Antimicrobial Effect of Moringa oleifera Leaves Extract on Foodborne Pathogens in Ground Beef. Int. J. Microbiol. Biotechnol. 2024, 9(1), 15-20. doi: 10.11648/ijmb.20240901.13

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    AMA Style

    Mwankunda LJ, Nyamete F, Kilima B. Antimicrobial Effect of Moringa oleifera Leaves Extract on Foodborne Pathogens in Ground Beef. Int J Microbiol Biotechnol. 2024;9(1):15-20. doi: 10.11648/ijmb.20240901.13

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  • @article{10.11648/ijmb.20240901.13,
      author = {Lucy Joshua Mwankunda and Frida Nyamete and Beautrice Kilima},
      title = {Antimicrobial Effect of Moringa oleifera Leaves Extract on Foodborne Pathogens in Ground Beef},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {9},
      number = {1},
      pages = {15-20},
      doi = {10.11648/ijmb.20240901.13},
      url = {https://doi.org/10.11648/ijmb.20240901.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.ijmb.20240901.13},
      abstract = {The study was done to examine the microbiological effects of Moringa oleifera as a preservation agent on ground beef held at 4°C for 72 hours. The study compared Moringa oleifera concentrations of 0.5%, 1.0%, 1.5%, and 2.0% to a preservative-free control group. This study measured Total bacteria counts (TBC), Total coliform count (TCC), Staphylococcus, Salmonella and E. coli. The study found a dose-dependent relationship between Moringa oleifera dosage and microbial populations. All Moringa oleifera concentrations demonstrated lower total bacterial counts (TBC) than the control group. The highest concentration (2.0%) showed the greatest reduction. This suggests Moringa oleifera could prevent ground beef bacterial growth. In TCC, Moringa oleifera reduced coliform bacteria better at higher concentrations. The concentrations of 1.5% and 2.0% reduced coliform counts significantly compared to the control group, demonstrating their efficacy in regulating them. Moringa oleifera showed dose-dependent antibacterial activity against Staphylococcus and E. coli. Increased preservative doses significantly reduced Staphylococcus and E. coli counts, suggesting they can improve food safety by reducing harmful microorganisms. Moringa oleifera appears to be an effective natural preservative, extending the shelf life and microbiological properties of ground beef under refrigerated storage. This study suggests employing natural preservatives to improve food safety and quality, which is important for the meat business.
    },
     year = {2024}
    }
    

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    AB  - The study was done to examine the microbiological effects of Moringa oleifera as a preservation agent on ground beef held at 4°C for 72 hours. The study compared Moringa oleifera concentrations of 0.5%, 1.0%, 1.5%, and 2.0% to a preservative-free control group. This study measured Total bacteria counts (TBC), Total coliform count (TCC), Staphylococcus, Salmonella and E. coli. The study found a dose-dependent relationship between Moringa oleifera dosage and microbial populations. All Moringa oleifera concentrations demonstrated lower total bacterial counts (TBC) than the control group. The highest concentration (2.0%) showed the greatest reduction. This suggests Moringa oleifera could prevent ground beef bacterial growth. In TCC, Moringa oleifera reduced coliform bacteria better at higher concentrations. The concentrations of 1.5% and 2.0% reduced coliform counts significantly compared to the control group, demonstrating their efficacy in regulating them. Moringa oleifera showed dose-dependent antibacterial activity against Staphylococcus and E. coli. Increased preservative doses significantly reduced Staphylococcus and E. coli counts, suggesting they can improve food safety by reducing harmful microorganisms. Moringa oleifera appears to be an effective natural preservative, extending the shelf life and microbiological properties of ground beef under refrigerated storage. This study suggests employing natural preservatives to improve food safety and quality, which is important for the meat business.
    
    VL  - 9
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Author Information
  • Department of Food Science and Agro-Processing Engineering, Sokoine University of Agriculture, Morogoro, Tanzania

  • Department of Food Science and Agro-Processing Engineering, Sokoine University of Agriculture, Morogoro, Tanzania

  • Department of Food Science and Agro-Processing Engineering, Sokoine University of Agriculture, Morogoro, Tanzania

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