International Journal of Microbiology and Biotechnology

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Evaluation of the Antimicrobial Activity of Cassia *Occidentalis Lin on Several Multi-Resistant Microbial Strains

Received: 18 December 2023    Accepted: 8 January 2024    Published: 23 January 2024
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Abstract

The aim of this work is to assess the antimicrobial potential of cassia occidentalis flax (Caesalpiniaceae) leaf extracts on drug-resistant microbial strains, in order to bring phytotherapeutic prescriptions into line with the potential biological activities of the chemical constituents present. Aqueous and hydro-ethanolic extracts were prepared according to the methods of Zirihi and Kra (2003). The antimicrobial activities were determined by measuring the rate of inhibition of microbial growth, diffusion on a solid medium and dilution in a liquid medium. In addition, the mode of action of the active extracts was determined by the ratio of the minimum fungicidal concentration to the minimum inhibitory concentration (MFC/MIC). Aqueous and hydroalcoholic extracts from the leaves of C. occidentalis have been shown to have antimicrobial activity. These extracts inhibited the proliferation of C. albicans strains resistant to nystatin. The activity of the aqueous extracts was similar to that obtained with the hydroalcoholic extracts. However, both types of extract showed no significant efficacy against S. aureus, E. coli and K. pneumoniae strains at the doses used (200 mg/ml; 100 mg/ml and 50 mg/ml) in all the pharmacological tests repeated during this study.

DOI 10.11648/j.ijmb.20240901.11
Published in International Journal of Microbiology and Biotechnology (Volume 9, Issue 1, April 2024)
Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Pharmaco-Multi-Resistance, Antimicrobial Activity, Fungicide, Cassia Occidentatlis

References
[1] Ahon M. G., Akapo-Akue J. M., Kra M. A., Ackah J. B., Zirihi N. G., Djaman J. A., 2011. Antifungal activity of the aqueous and hydro-alcoholic extracts of Terminalia superba Engl. on the in vitro growth of clinical isolates of pathogenic fungi. Agric. Biol. J. N. Am., 2(2): 250-257.
[2] Andrianarison E. R., Rakotosaona R., Andrianaivoravelona O. J., Adrianarison R. J., Handrinirina J. E., et Andrianary P. A. Screening phytochimique et isolement par voie bioguidée de substances à activitéantibactérienne d’extraits de Cassia occidentalis Sond récoltée à Maevatanàna. (ISSN), 4, (2015), 2410-0315.
[3] Duraffourd C., D’Hervicourt L. et Lapraz J. C., Cahiers de phytothérapie clinique. Examen de laboratoire galénique, Eléments Thérapeutiques Synergiques. 2ème Edition, Masson (Paris), (1990), 87 p.
[4] Fotin, A., Cheng, Y., Sliz, P., Grigorieff, N., Harrison, S. C., Kirchhausen, T., & Walz, T. (2004). Molecular model for a complete clathrin lattice from electron cryomicroscopy. Nature, 432(7017), 573-579.
[5] Livermore D., M. bêta-lactamase mediated resistance: past, present and future, J. Infect. Dis. Soc., 6, (1995), 75-83.
[6] Leroux P. et Credet A., 1978. Document sur l’étude de l’activité des fongicides. INRA. Versaille France, 12p.
[7] Muthu C. Ayanar M. Raja N. Ignacimuthu S (2006) Medecinal plants used by traditional healers in Kancheepuram district of Tamil Nadu, India J Ethno Ethnomed 2: 43.
[8] OMS (2002) Rapport sur la médecine traditionnelle: besoins et potentiel 4: 6 p.
[9] Ponce A. G., Fritz R., del Valle C., and Roura S. I., Antibacterial activity of essential oils on the native microflora of organic Swiss chard. Society of Food Science and Technology (Elsevier), 36, (2003), 679-684.
[10] Savard P. Y., Caractérisation structurale et dynamique de la bêta-lactamase TEM-1 de la bactérie Escherichia coli par RMN liquide, Philosophiae Doctor de Biochimie et de Microbiologie, Faculté des sciences et de Génie, Université Laval, Québec, (2003) 224 p.
[11] Scalbert A., Antimicrobial properties of tannins. Phytochemistry, 30, (1991), 3875-3883.
[12] Yano Y, Satomi M, Oikawa H (2006) Antimicrobial effet of spices and herbs on vibrio parahaemolyticus. Int J Food Microbiol 111(1): 6-11.
[13] Zekeya, N., Ouma, J., Chacha, M., Ndossi, H., & Mbega, E. (2020). Understanding factors influencing distribution and density of a micro-Lepidoptera moth, Tuta absoluta (Gelechiidae) and its impact in tomato agroecological zones of Tanzania. Bioscience Research.
[14] Zirihi G. N. et Kra A. K. M., 2003. Évaluation de l’activité antifongique de Microglossa pyrifolia (Lam.) O. Ktze (Asteraceae) «PYMI» sur la croissance in vitro de Candida albicans. Revue médicale et pharm. Afric 17: 1–19.
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    Ngassaki, R., Ampa, R., Tchiyamba, P. L., Assiana, D. O. E., Ahombo, G. (2024). Evaluation of the Antimicrobial Activity of Cassia *Occidentalis Lin on Several Multi-Resistant Microbial Strains. International Journal of Microbiology and Biotechnology, 9(1), 1-6. https://doi.org/10.11648/j.ijmb.20240901.11

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    ACS Style

    Ngassaki, R.; Ampa, R.; Tchiyamba, P. L.; Assiana, D. O. E.; Ahombo, G. Evaluation of the Antimicrobial Activity of Cassia *Occidentalis Lin on Several Multi-Resistant Microbial Strains. Int. J. Microbiol. Biotechnol. 2024, 9(1), 1-6. doi: 10.11648/j.ijmb.20240901.11

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    AMA Style

    Ngassaki R, Ampa R, Tchiyamba PL, Assiana DOE, Ahombo G. Evaluation of the Antimicrobial Activity of Cassia *Occidentalis Lin on Several Multi-Resistant Microbial Strains. Int J Microbiol Biotechnol. 2024;9(1):1-6. doi: 10.11648/j.ijmb.20240901.11

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  • @article{10.11648/j.ijmb.20240901.11,
      author = {Raoul Ngassaki and Raoul Ampa and Pierrette Lokanga Tchiyamba and Darrel Ornelle Elion Assiana and Gabriel Ahombo},
      title = {Evaluation of the Antimicrobial Activity of Cassia *Occidentalis Lin on Several Multi-Resistant Microbial Strains},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {9},
      number = {1},
      pages = {1-6},
      doi = {10.11648/j.ijmb.20240901.11},
      url = {https://doi.org/10.11648/j.ijmb.20240901.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20240901.11},
      abstract = {The aim of this work is to assess the antimicrobial potential of cassia occidentalis flax (Caesalpiniaceae) leaf extracts on drug-resistant microbial strains, in order to bring phytotherapeutic prescriptions into line with the potential biological activities of the chemical constituents present. Aqueous and hydro-ethanolic extracts were prepared according to the methods of Zirihi and Kra (2003). The antimicrobial activities were determined by measuring the rate of inhibition of microbial growth, diffusion on a solid medium and dilution in a liquid medium. In addition, the mode of action of the active extracts was determined by the ratio of the minimum fungicidal concentration to the minimum inhibitory concentration (MFC/MIC). Aqueous and hydroalcoholic extracts from the leaves of C. occidentalis have been shown to have antimicrobial activity. These extracts inhibited the proliferation of C. albicans strains resistant to nystatin. The activity of the aqueous extracts was similar to that obtained with the hydroalcoholic extracts. However, both types of extract showed no significant efficacy against S. aureus, E. coli and K. pneumoniae strains at the doses used (200 mg/ml; 100 mg/ml and 50 mg/ml) in all the pharmacological tests repeated during this study.
    },
     year = {2024}
    }
    

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    AB  - The aim of this work is to assess the antimicrobial potential of cassia occidentalis flax (Caesalpiniaceae) leaf extracts on drug-resistant microbial strains, in order to bring phytotherapeutic prescriptions into line with the potential biological activities of the chemical constituents present. Aqueous and hydro-ethanolic extracts were prepared according to the methods of Zirihi and Kra (2003). The antimicrobial activities were determined by measuring the rate of inhibition of microbial growth, diffusion on a solid medium and dilution in a liquid medium. In addition, the mode of action of the active extracts was determined by the ratio of the minimum fungicidal concentration to the minimum inhibitory concentration (MFC/MIC). Aqueous and hydroalcoholic extracts from the leaves of C. occidentalis have been shown to have antimicrobial activity. These extracts inhibited the proliferation of C. albicans strains resistant to nystatin. The activity of the aqueous extracts was similar to that obtained with the hydroalcoholic extracts. However, both types of extract showed no significant efficacy against S. aureus, E. coli and K. pneumoniae strains at the doses used (200 mg/ml; 100 mg/ml and 50 mg/ml) in all the pharmacological tests repeated during this study.
    
    VL  - 9
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Author Information
  • Laboratory of Cellular and Molecular Biology, Marien Ngouabi University, Brazzaville, Congo; Microbiological Laboratory, Quality Control Department of the BIOCARE Pharmaceutical Industry, Brazzaville, Congo

  • Laboratory of Cellular and Molecular Biology, Marien Ngouabi University, Brazzaville, Congo

  • Institut de Recherche en Sciences de la Santé (IRSSA), Ministry of Higher Education, Scientific Research and Technological Innovation, Brazzaville, Congo

  • Laboratory of Cellular and Molecular Biology, Marien Ngouabi University, Brazzaville, Congo

  • Laboratory of Cellular and Molecular Biology, Marien Ngouabi University, Brazzaville, Congo

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