CRISPR-Mediated Metabolic Engineering for Sustainable Biomanufacturing :: Special Issue

01About This Special Issue

The rapid transition toward a global circular bioeconomy has intensified the demand for robust microbial and plant cell factories capable of producing biofuels, biopolymers, bulk chemicals, and high-value therapeutics sustainably. Traditional genetic engineering methods are often limited by low precision, slow development cycles, and an inability to execute complex, multi-locus genomic edits simultaneously.

The emergence of CRISPR-based synthetic biology toolkits—including Cas9, Cas12, CRISPR interference (CRISPRi), CRISPR activation (CRISPRa), and base editing—has fundamentally revolutionized metabolic engineering. These advanced systems enable precise, scarless, and multiplexed genetic manipulation, allowing researchers to rapidly redirect metabolic fluxes, eliminate competing pathways, and optimize host tolerances.

This Special Issue aims to gather cutting-edge research and comprehensive reviews highlighting innovations in CRISPR-mediated engineering across model and non-model microbial or plant chassis. By capturing novel tools, automated workflows, and practical scale-up strategies, this collection will serve as a definitive benchmark for next-generation green biomanufacturing.

We welcome original research articles, short communications, and comprehensive reviews including, but not limited to, the following themes:

1. Development of novel CRISPR toolkits (e.g., Cas12/Cas13 variants, base editors, prime editing) for metabolic pathway adjustments.
2. Multiplex genome editing and combinatorial transcriptional tuning using CRISPRi and CRISPRa.
3. Domestication and engineering of non-model microorganisms (e.g., cyanobacteria, fungi, environmental isolates) for industrial biosynthesis.
4. Redirection of metabolic flux to optimize yields of biofuels, bioplastics, organic acids, and precision therapeutics.
5. Integration of CRISPR workflows with AI-driven biology platforms, metabolic modeling, and biofoundry automation.
6. Addressing industrial implementation barriers, including genetic instability during scale-up, metabolic stress tolerance, and biocontainment/biosafety mechanisms.

02Meet the Guest Editors

Our distinguished editors bring deep subject-matter expertise to curate high-quality research and ensure a rigorous peer-review process.

Lead Guest Editor

Sreelatha Macherla

Department of Pharmacy, Malineni Perumallu Pharmacy College, Guntur, India

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04Published Articles

This special issue is now open for submission. Submit your manuscript here .

International Journal of Microbiology and Biotechnology