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Characterization of Stress Tolerant High Potential Ethanol Producing Yeast from Agro-Industrial Waste
Md. Fakruddin,
Md. Ariful Islam,
Md. Abdul Quayum,
Monzur Morshed Ahmed,
Nayuum Chowdhury
Issue:
Volume 1, Issue 2, July 2013
Pages:
24-34
Received:
13 May 2013
Published:
10 July 2013
Abstract: Bioethanol or biofuel as an alternative to fossil fuels has been expanded in the last few decades in the whole world. Use of bioethanol as a renewable transportation fuel will minimize the amounts of fossil-derived carbon dioxide (CO2) to the Earth’s atmosphere. Yeast is the most favorite organism for ethanol production because of its diverse substrate specificity and ease of production of ethanol under anaerobic condition. The main objective of this research work was to isolate & characterize stress tolerant, high potential ethanol producing yeast strains from agro industrial waste. In total 4 yeast isolates have been characterized on the basis of morphological and physico-chemical characters. Based on morphological appearance of vegetative cell under microscope, ascospore production, colony character and physico-chemical characters all the strains was identified to be Yeast. Phylogenetic identification by DNA sequencing confirmed that the strain P is Saccharomyces Unisporus, strain C is Saccharomyces cerevisiae, strain T is Saccharomyces cerevisiae & strain DB2 is Candida piceae. Most of the strains were thermotolerant, pH tolerant, ethanol tolerant as well as osmotolerant. They were resistant to cycloheximide at 0.0015g/100ml concentration, hydrogen peroxide (0.50%), Chloramphenicol (30µg/disc) but growth was inhibited in the presence of 1% acetic Acid. The strains P, C & T showed good Invertase activity & only the T strain was capable of producing killer toxin. They were capable of fermenting glucose, fructose, sucrose, amylose & trehalose. Ethanol producing capability of the strains was studied using sugarcane molasses as substrate. The bioethanol production capacity of the yeasts were found to be 15%, 14.5%, 12% & 8.15% for P, C, T & DB2 respectively at pH 6.0, 30oC temperature in media with 5.5% initial reducing sugar concentration in shaking condition. Pilot scale ethanol production by P strain was 13.10%, C strain 11.15%, T strain 9.80% & DB2 strains 7.85% at 60 hours. These strains could be potential for ethanol production from cane molasses.
Abstract: Bioethanol or biofuel as an alternative to fossil fuels has been expanded in the last few decades in the whole world. Use of bioethanol as a renewable transportation fuel will minimize the amounts of fossil-derived carbon dioxide (CO2) to the Earth’s atmosphere. Yeast is the most favorite organism for ethanol production because of its diverse subst...
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Assessment of Biochemical Compositions and Mineral Contents of Carapace of Some Important Commercially Crustaceans and Mollusks Organisms from Egyptian and Saudi Arabia Coasts as a New Animal Feed
Issue:
Volume 1, Issue 2, July 2013
Pages:
35-43
Received:
1 June 2013
Published:
10 July 2013
Abstract: Carapace of commercial crustaceans species (mantis shrimps ; Erugosquilla. massavensis, the penaeid shrimps ; Peaneus. semisulcatus ; Metapenaeus monoceros and blue crab; Portunus pelagicus ) and commercial mollusks (cuttlefish ; Sepia and bivalve mollusks; Cardium edule) were chosen for this study to assess carbohydrates, protein, lipids and to estimate their Fe, Cu, Co, Hg, Pb and Cd contents, as an attempt to use the carapace of marine invertebrates organisms as a cheap protein animal feed. Additionally, to investigate the interrelation between the exoskeleton of these marine animals and water pollution. In the terms of nutritive quality, the present results show that the carapace of both sex of E. massavensis was the richest nutritive source compared to that of all studied samples. Furthermore, the recorded data indicate that the exoskeleton of all studied crustaceans organisms and the internal shell of cephalopod mollusks had higher nutritive contents than the exoskeleton of bivalve mollusks. Concerning with heavy metal bioaccumulation, the present study show that the carapace in both sexes of E. massavensis accumulated higher percentages of the determined metals than those of other studied crustaceans species. On the other hand, Sepia spp. exhibited the lowest levels of the studied metals comparable to crustaceans and C. edule species. While, all species of C. edule accumulated the higher concentrations of Fe, Co, Pb and Cd in their shells than those in all studied crustaceans species. In this respect, the values of Metal Pollution Index (MPI) of studied essential elements in all studied species were higher than that of non- essential elements. Moreover, the highest values of essential and non-essential elements were recorded in all E. massavensis and C. edule samples respectively. While, the lowest level was detected in Sepia spp. followed by crab species. This primary work is an attempt to pursue further research in this line and will also be useful in the preparation of value added sea foods; the findings of the current study may help to select the appropriate species for animal feed. Moreover, the present study suggests that the exoskeleton of crustaceans and bivalves mollusks can be used as indicator of water pollution and this suggest their tendency to detoxification mechanism of heavy metals as a mean of protection. This in turn suggests that a close monitoring program is need to ensure the safety of these crustacean and mollusks species as a food source for animals.
Abstract: Carapace of commercial crustaceans species (mantis shrimps ; Erugosquilla. massavensis, the penaeid shrimps ; Peaneus. semisulcatus ; Metapenaeus monoceros and blue crab; Portunus pelagicus ) and commercial mollusks (cuttlefish ; Sepia and bivalve mollusks; Cardium edule) were chosen for this study to assess carbohydrates, protein, lipids and to es...
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Pharmacological Potentials of Melia Azedarach L. - A Review
Azam M. M.,
Mamun-Or-Rashid A. N. M.,
Towfique N. M.,
Sen M. K.,
Nasrin. S
Issue:
Volume 1, Issue 2, July 2013
Pages:
44-49
Received:
21 June 2013
Published:
10 July 2013
Abstract: Medicinal plants are widely used by the traditional medicinal practitioners to cure different diseases due to their world-wide availability and fewer side effects. Melia azedarach L. (Ghoda Neem locally) belongs to the family Meliaceae, is a highly significant medicinal plant found almost everywhere in Bangladesh. Traditionally, different parts such as leaf, flower, seed, fruit, and young branches of have been used for the treatment of malaria, diabetes, purgative, cough, skin disease, and so on. Experimental and clinical studies prove that it has antioxidant, antimicrobial, anti-inflammatory, cardioprotective, analgesic, anticancer, antiulcer, antipyretic, antiplasmodial and male contraceptive properties. For the last few decades or so, extensive research work has been done to prove its biological activities and pharmacology of its extracts. The present compendium review will focus on is traditional uses and pharmaceutical activities found on different scientific research and reports. This review also includes reports on phytochemistry, taxonomy, morphology, monographs, distribution and toxic effects of M. azedarach.
Abstract: Medicinal plants are widely used by the traditional medicinal practitioners to cure different diseases due to their world-wide availability and fewer side effects. Melia azedarach L. (Ghoda Neem locally) belongs to the family Meliaceae, is a highly significant medicinal plant found almost everywhere in Bangladesh. Traditionally, different parts suc...
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