1. Introduction
Honey is a natural apicultural product produced by bees from floral nectar. It is widely valued for its nutritional, therapeutic, and economic properties. Its quality depends on several factors, including botanical origin, as well as conditions of production, harvesting, processing, and storage
| [1] | Albu A, Bora FD, Cucu-Man S-M, et al (2025) Assessing Honey Quality: A Focus on Some Physicochemical Parameters of Honey from Iasi County (Romania). Agriculture 15: 333.
https://doi.org/10.3390/agriculture15030333 |
| [2] | Rysha A, Kastrati G, Biber L, et al (2022) Evaluating the Physicochemical Properties of Some Kosovo’s and Imported Honey Samples. Applied Sciences 12: 629.
https://doi.org/10.3390/app12020629 |
[1, 2]
. Honey is recognized for its numerous health benefits, particularly its antibacterial, antioxidant, anti-inflammatory, antimutagenic, and anticarcinogenic properties
| [3] | Vázquez-Quiñones CR, Moreno-Terrazas R, Natividad-Bonifacio I, et al. (2018) Microbiological assessment of honey in Mexico. Revista Argentina de Microbiología. 50: 75-80.
https://doi.org/10.1016/j.ram.2017.04.005 |
| [4] | Al-Waili N, Salom K, Al-Ghamdi A, Ansari MJ (2012) Antibiotic, Pesticide, and Microbial Contaminants of Honey: Human Health Hazards. The Scientific World Journal 2012: 1-9.
https://doi.org/10.1100/2012/930849 |
[3, 4]
. These properties are associated with its complex chemical composition, predominantly consisting of simple sugars—mainly glucose and fructose—but also including minor constituents such as enzymes, minerals, amino acids, vitamins, and polyphenols
| [5] | Luca L, Pauliuc D, Oroian M (2024) Honey microbiota, methods for determining the microbiological composition and the antimicrobial effect of honey - A review. Food Chemistry: X 23: 101524. https://doi.org/10.1016/j.fochx.2024.101524 |
| [6] | Guerzou M, Aouissi HA, Guerzou A, et al (2021) From the Beehives: Identification and Comparison of Physicochemical Properties of Algerian Honey. Resources 10: 94.
https://doi.org/10.3390/resources10100094 |
[5, 6]
. The water content of honey (generally ≤ 20%) plays a critical role in its stability, influencing its viscosity, crystallization, fermentation, and microbiological quality
| [7] | Mohamad Ghazali NS, Yusof YA, Mohd Ghazali H, et al (2021) Effect of surface area of clay pots on physicochemical and microbiological properties of stingless bee (Geniotrigona thoracica) honey. Food Bioscience 40: 100839.
https://doi.org/10.1016/j.fbio.2020.100839 |
[7]
.
Other parameters, such as reducing sugar content, sucrose content, and hydroxymethylfurfural (HMF) concentration, are also essential indicators of honey quality and authenticity
| [2] | Rysha A, Kastrati G, Biber L, et al (2022) Evaluating the Physicochemical Properties of Some Kosovo’s and Imported Honey Samples. Applied Sciences 12: 629.
https://doi.org/10.3390/app12020629 |
| [8] | Gajek M, Moj K, Wysocki P, et al (2026) Analysis of Changes in Selected Physicochemical Parameters and Elemental Composition of Honey as a Result of Adulteration with Sugar Additives. Foods 15: 562. https://doi.org/10.3390/foods15030562 |
[2, 8]
. Furthermore, honey is characterized by an acidic pH (ranging from 3.2 to 4.5), which also limits the growth of microorganisms
.
Despite these intrinsic properties that are unfavorable to microbial proliferation, honey may harbor a microbiota of diverse origin. This microbiota derives from primary sources, such as nectar, pollen, and the bees’ own microbiota, as well as from secondary sources related to extraction, handling, and storage conditions
. Inadequate hygiene practices during extraction, handling, and packaging can lead to contamination by various microorganisms, thereby compromising honey quality
.
The major honey-producing countries include China, the United States, and Argentina
| [12] | Bakary C, Masse D, Ibrahim K, Guichard BL (2019) Microbiological quality, physicochemical properties, and sensory profile of honeys from the Worodougou region, Côte d’Ivoire. ESJ 15: https://doi.org/10.19044/esj.2019.v15n30p72 |
[12]
. In addition, during the same period, countries such as Turkey, Iran, Ethiopia, Argentina, the United States of America, Russia, Ukraine, India, and Mexico ranked among the top ten global honey producers
. However, in developing countries such as Guinea, the apiculture sector remains largely traditional. Artisanal methods of production and marketing, often characterized by a lack of standardization and control, may affect honey quality, particularly in terms of physicochemical deterioration and microbiological contamination.
In the Macenta region, where honey production represents an important economic activity, few scientific studies have been conducted to assess its quality. A better understanding of the physicochemical and microbiological characteristics of locally produced honey is therefore necessary to ensure its safety, enhance its value, and facilitate market access. Thus, a key question arises: what is the physicochemical and microbiological quality of honey produced and marketed in Macenta, and to what extent does it comply with international food safety standards?
In this context, the present study aimed to evaluate the physicochemical and microbiological quality of honey produced and marketed in Macenta, in order to assess its compliance with existing standards and contribute to improving its sanitary quality.
2. Materials and Methods
2.1. Study Area
This study was conducted in the urban municipality of Macenta, located in the Nzérékoré Region in Forest Guinea (
Figure 1). This area was selected due to its significant role in the local production and commercialization of honey. Its geographical location, combined with high consumption of the product by the population, justifies the relevance of assessing the physicochemical and microbiological quality of honey marketed in the region.
Figure 1. Location of Macenta Prefecture, Guinea.
2.2. Sampling
A stratified random sampling approach was implemented to ensure the representativeness of samples according to production volume and the attendance of sales points. A total of 26 honey samples, each weighing approximately 500 g, were collected from various retail outlets in the urban municipality of Macenta in September 2025. Sampling was carried out aseptically using sterile containers. The samples were then transported in an insulated cooler (
Figure 2) to preserve their physicochemical and microbiological characteristics until analysis at the laboratory of the National Office for Quality Control (ONCQ) in Conakry.
Figure 2. Collection and packaging of honey samples.
2.3. Physicochemical Analysis
Physicochemical analyses of honey were performed using standard methods. Moisture content was determined by weight loss after drying in an oven at 100-105°C until constant weight was achieved. The pH was measured using a pH meter directly immersed in the sample
| [13] | Babarinde GO, Babarinde SA, Adegbola DO, Ajayeoba SI (2011) Effects of harvesting methods on physicochemical and microbial qualities of honey. J Food Sci Technol 48: 628-634.
https://doi.org/10.1007/s13197-011-0329-9 |
[13]
. Sugar content was measured using a portable refractometer
| [8] | Gajek M, Moj K, Wysocki P, et al (2026) Analysis of Changes in Selected Physicochemical Parameters and Elemental Composition of Honey as a Result of Adulteration with Sugar Additives. Foods 15: 562. https://doi.org/10.3390/foods15030562 |
[8]
. Protein content was determined using the Kjeldahl method
| [2] | Rysha A, Kastrati G, Biber L, et al (2022) Evaluating the Physicochemical Properties of Some Kosovo’s and Imported Honey Samples. Applied Sciences 12: 629.
https://doi.org/10.3390/app12020629 |
[2]
. Total ash content was obtained by incineration at 650-850°C
| [1] | Albu A, Bora FD, Cucu-Man S-M, et al (2025) Assessing Honey Quality: A Focus on Some Physicochemical Parameters of Honey from Iasi County (Romania). Agriculture 15: 333.
https://doi.org/10.3390/agriculture15030333 |
[1]
. This methodology allowed for a comprehensive characterization of the physicochemical properties of honey samples.
2.4. Microbiological Analysis
Microbiological analyses of honey were performed using standard methods adapted to each target microorganism (
Figure 3). Total aerobic mesophilic flora (TAMF) was incubated on Plate Count Agar (PCA) at 30°C for 72 h. Total coliforms (TC) and thermotolerant coliforms (TTC) were enumerated on Violet Red Bile Lactose Agar (VRBL) at 37°C and 44°C, respectively, for 24 h. Yeasts and molds (YM) were enumerated on Potato Dextrose Agar (PDA) at 30°C for 72 to 120 h. Sulfite-reducing anaerobes (SRA) were cultured on VRBL at 46°C for 24 h. Isolation of
Salmonella spp. was carried out using the classical steps of pre-enrichment, selective enrichment, and isolation
| [14] | Nzeh J, Quansah L, Dufailu OA (2022) Physicochemical properties of imported and locally produced honey did not translate into its microbial quality and antibacterial activity. Discov Food 2: 24. https://doi.org/10.1007/s44187-022-00024-6 |
[14]
.
Figure 3. Microbiological analyses of honey.
2.5. Statistical Analysis
Data from physicochemical and microbiological analyses were processed using STATA software (version 15.1). Measured parameters were described using descriptive statistics, including means, standard deviations, minimum, and maximum values. To assess the compliance of samples with WHO standards, statistical comparison tests were performed, with a significance level set at p < 0.05. This approach enabled the identification of significant variations and highlighted non-compliant parameters that may pose a risk to food safety.
3. Results
3.1. Physicochemical Parameters
Figure 4 presents the physicochemical characteristics of twenty-six (26) honey samples collected in Macenta (Guinea). Moisture content ranged from 14.09% to 17.95%, remaining within international standards (< 20%), indicating good preservation potential. However, the highest values could, under inadequate storage conditions, favor fermentation processes. Protein content, ranging from 0.62% to 1.25%, reflects low variability related to floral origin while remaining within expected values for honey. Total ash content varied from 0.24% to 1.21%, indicating heterogeneity in mineral composition, likely influenced by botanical origin and environmental conditions. Sugar content, ranging from 62.50% to 76.25%, reflects a high carbohydrate concentration, which contributes to honey stability and preservation. Finally, pH values ranged from 3.63 to 5.04, confirming the acidic nature of honey, which contributes to microbial inhibition and overall product stability.
Figure 4. Results of the physicochemical analysis of honey.
3.2. Microbiological Parameters
Figure 5 presents the microbiological results of twenty-six (26) honey samples. The majority of samples (E1 to E7, E10 to E20, and E23 to E26) exhibited a low microbial load, with total aerobic mesophilic flora (TAMF) values around 1.3 × 10
2 CFU/mL. These samples were also characterized by the absence or very low levels of total and thermotolerant coliforms, sulfite-reducing anaerobes, and Salmonella spp. Yeasts and molds were generally low, indicating satisfactory microbiological quality. In contrast, four samples (E8, E9, E21, and E22) showed high TAMF counts (≥ 10
4 CFU/mL), accompanied by total coliforms (≈ 10
2 CFU/mL) and thermotolerant coliforms (≈ 10
1 CFU/mL), as well as high levels of yeasts and molds (≥ 2 × 10
4 CFU/mL). These results suggest environmental contamination, most likely related to inadequate hygiene conditions during production, handling, or storage. Furthermore, the absence of sulfite-reducing anaerobes and Salmonella spp. in all samples represents a favorable indicator of sanitary safety with respect to major pathogenic microorganisms. Overall, the findings indicate a generally satisfactory but heterogeneous microbiological quality, highlighting the need to strengthen good hygiene practices and improve honey storage conditions.
Figure 5. Results of the microbiological analysis of honey samples.
4. Discussion
4.1. Physicochemical Parameters
Moisture content ranged from 14.09 to 17.95%, falling within the 14-24.7% range reported by Nabeneza S et al.,
| [15] | Nabeneza S, Porphyre V, Davrieux F (2015) Characterization of honeys from the Indian Ocean region by near-infrared spectroscopy: feasibility study. Rev Elev Med Vet Pays Trop 67: 130-132. https://doi.org/10.19182/remvt.10181 |
[15]
for Indian Ocean honeys analyzed using near-infrared spectroscopy. The maximum value obtained (17.95%) is close to the mean value of 17.44 g/100 g reported by Ucuncu O et al.,
for Turkish multifloral honeys. However, the values obtained in this study are slightly lower than those reported by Polatidou K et al.,
| [17] | Polatidou K, Nouska C, Tananaki C, et al (2025) Physicochemical and Rheological Characteristics of Monofloral Honeys—Kinetics of Creaming-Crystallization. Foods 14: 1835.
https://doi.org/10.3390/foods14101835 |
[17]
(14.60-18.10%) for monofloral honeys, as well as those reported by Oroian M et al.,
(17.12-18.52%) regarding the chemical composition and textural properties of honey. Nevertheless, these differences were not statistically significant (p > 0.05), suggesting relative homogeneity in moisture content across studies. The observed variations may be attributed to climatic conditions, botanical origin of nectar, and analytical methods used.
Protein contents (0.62-1.25%) were generally higher than those reported in the literature by White JW et al.,
, who observed an average value of 169 mg/100 g (i.e., 0.169%) across 740 honey samples, while Cucu A-A et al.,
| [20] | Cucu A-A, Baci G-M, Moise AR, et al (2021) Towards a Better Understanding of Nutritional and Therapeutic Effects of Honey and Their Applications in Apitherapy. Applied Sciences 11: 4190. https://doi.org/10.3390/app11094190 |
[20]
and Khan SU et al.,
reported mean values close to 0.5%. A lower value (~0.3%) was reported by Zammit Young G-W et al.,
. Statistical analysis revealed that the differences between the present results and those reported in the literature were significant (p < 0.05). The relatively higher levels observed may be attributed to increased pollen content, which represents the main protein source in honey, as well as to botanical origin and environmental conditions. Variations related to analytical methods cannot be excluded. These findings suggest a specific composition of the studied honeys, likely influenced by floral origin and filtration degree.
Ash contents (0.24-1.21%) were slightly higher than those reported by Guerzou M et al.,
| [6] | Guerzou M, Aouissi HA, Guerzou A, et al (2021) From the Beehives: Identification and Comparison of Physicochemical Properties of Algerian Honey. Resources 10: 94.
https://doi.org/10.3390/resources10100094 |
[6]
for Algerian honeys (0.06-0.48%) and generally higher than those reported by Lewoyehu M et al.,
| [23] | Lewoyehu M, Amare M (2019) Comparative Assessment on Selected Physicochemical Parameters and Antioxidant and Antimicrobial Activities of Honey Samples from Selected Districts of the Amhara and Tigray Regions, Ethiopia. International Journal of Food Science 2019: 1-10.
https://doi.org/10.1155/2019/4101695 |
[23]
for Ethiopian honeys (0.08 ± 0.00 to 0.45 ± 0.03%). However, they were close to values reported by Rababah T et al.,
| [24] | Rababah T, Al-U’datt M, Naqresh A, et al (2024) Effect of Temperature and Time on the Physicochemical and Sensory Properties of Crystallized Honey. ACS Omega 9: 20243-20252.
https://doi.org/10.1021/acsomega.4c00570 |
[24]
for crystallized honeys (1.14-1.17%). Statistical analysis showed that differences with Guerzou M et al.,
| [6] | Guerzou M, Aouissi HA, Guerzou A, et al (2021) From the Beehives: Identification and Comparison of Physicochemical Properties of Algerian Honey. Resources 10: 94.
https://doi.org/10.3390/resources10100094 |
[6]
and Bonte F et al.,
were significant (p < 0.05), whereas no significant difference was observed with Waworuntu J
. This mineral richness may be attributed to the botanical origin of the honeys and environmental conditions in which they were produced.
Sugar content (62.50-76.25%) was lower than values reported by Bonte F et al.,
for honeys (~80%), by Waworuntu J
for Apis mellifera L. honeys (73.40-83.9%), and by Gajek M et al.,
| [8] | Gajek M, Moj K, Wysocki P, et al (2026) Analysis of Changes in Selected Physicochemical Parameters and Elemental Composition of Honey as a Result of Adulteration with Sugar Additives. Foods 15: 562. https://doi.org/10.3390/foods15030562 |
[8]
(81.5-82.5°Brix). Similarly, Zammit Young G-W et al.,
reported a carbohydrate content of approximately 80%, slightly higher than the values observed in this study. Statistical analysis indicated that these differences were significant (p < 0.05), confirming that the honeys in this study exhibited lower sugar levels compared to those reported in the literature. These variations may be linked to botanical origin, degree of maturity, water content, or environmental conditions.
pH values (3.63-5.04) were generally close to those reported by Bonte F et al.,
(3.5-6.0) and Raweh HAS et al.,
| [27] | Raweh HSA, Badjah-Hadj-Ahmed AY, Iqbal J, Alqarni AS (2023) Physicochemical Composition of Local and Imported Honeys Associated with Quality Standards. Foods 12: 2181.
https://doi.org/10.3390/foods12112181 |
[27]
(4.5-5.0). They were higher than the value of 4 reported by Werner A et al.,
, and slightly higher than those observed by Khan SU et al.,
(2.4-4.7). Statistical analysis showed that some differences, particularly with Luca L et al.,
| [30] | Luca L, Pauliuc D, Ursachi F, Oroian M (2025) Physicochemical parameters, microbiological quality, and antibacterial activity of honey from the Bucovina region of Romania. Sci Rep 15: 4358. https://doi.org/10.1038/s41598-025-88613-0 |
[30]
and Lewoyehu M et al.,
| [23] | Lewoyehu M, Amare M (2019) Comparative Assessment on Selected Physicochemical Parameters and Antioxidant and Antimicrobial Activities of Honey Samples from Selected Districts of the Amhara and Tigray Regions, Ethiopia. International Journal of Food Science 2019: 1-10.
https://doi.org/10.1155/2019/4101695 |
[23]
, were significant (p < 0.05), while those with Raweh HAS et al.,
| [27] | Raweh HSA, Badjah-Hadj-Ahmed AY, Iqbal J, Alqarni AS (2023) Physicochemical Composition of Local and Imported Honeys Associated with Quality Standards. Foods 12: 2181.
https://doi.org/10.3390/foods12112181 |
[27]
and Damto T et al.,
| [31] | Damto T, Kebebe D, Gemeda M (2023) Physicochemical properties and microbial qualities of honey produced by a stingless bee (Meliponula beccarii L.) in the Oromia region, Ethiopia. Cogent Food & Agriculture 9: 2258784.
https://doi.org/10.1080/23311932.2023.2258784 |
[31]
were not significant. These pH variations may be related to botanical origin and water content, both of which are known to influence acidity and microbiological stability.
4.2. Microbiological Parameters
Total aerobic mesophilic flora counts obtained in the present study (1.5 × 10
1 to 1.08 × 10
4 CFU/mL) are generally higher than those reported by Iurlina MO et al.,
, who observed counts below 10
3 CFU/g across 70 honey samples analyzed. Lower values were also reported by Luca L et al.,
| [30] | Luca L, Pauliuc D, Ursachi F, Oroian M (2025) Physicochemical parameters, microbiological quality, and antibacterial activity of honey from the Bucovina region of Romania. Sci Rep 15: 4358. https://doi.org/10.1038/s41598-025-88613-0 |
[30]
in honeys from the Bukovina region (Romania), with counts ranging from 10 to 5 × 10
2 CFU/g. In contrast, the contamination levels observed in this study remain lower than those reported by Damto T et al.,
| [31] | Damto T, Kebebe D, Gemeda M (2023) Physicochemical properties and microbial qualities of honey produced by a stingless bee (Meliponula beccarii L.) in the Oromia region, Ethiopia. Cogent Food & Agriculture 9: 2258784.
https://doi.org/10.1080/23311932.2023.2258784 |
[31]
, who recorded values between 1.9 × 10
3 and 2.55 × 10
4 CFU/mL in honey produced by Meliponula beccarii in Ethiopia. These variations may be explained by differences in harvesting, handling, processing, and storage conditions, as well as by botanical origin and beekeeping practices.
Total coliform counts (0 to 1.08 × 10
2 CFU/mL) and thermotolerant coliforms (0 to 2.8 × 10
1 CFU/mL) observed in the present study are higher than those reported by Bakary C et al.,
| [12] | Bakary C, Masse D, Ibrahim K, Guichard BL (2019) Microbiological quality, physicochemical properties, and sensory profile of honeys from the Worodougou region, Côte d’Ivoire. ESJ 15: https://doi.org/10.19044/esj.2019.v15n30p72 |
[12]
, who detected no coliform contamination in honey samples from the Worodougou region (Côte d’Ivoire). Similarly, a recent study by Bruxel F et al.,
on commercial honeys reported the absence of total coliforms in all analyzed samples. Furthermore, Fernández LA et al.,
| [32] | Fernández LA, Ghilardi C, Hoffmann B, et al (2017) Microbiological quality of honey from the Pampas Region (Argentina) throughout the extraction process. Revista Argentina de Microbiología 49: 55-61.
https://doi.org/10.1016/j.ram.2016.05.010 |
[32]
, in a study on the microbiological quality of honey from the Pampas region (Argentina), did not observe any fecal coliforms in the examined samples. These differences suggest that the presence of coliforms in the present study may result from post-harvest contamination associated with less stringent hygiene practices during honey handling, processing, or packaging.
Yeast and mold counts (2.0 to 2.25 × 10
4 CFU/mL) obtained in this study are markedly higher than those reported by Luca L et al.,
| [30] | Luca L, Pauliuc D, Ursachi F, Oroian M (2025) Physicochemical parameters, microbiological quality, and antibacterial activity of honey from the Bucovina region of Romania. Sci Rep 15: 4358. https://doi.org/10.1038/s41598-025-88613-0 |
[30]
, who recorded values ranging from 1.68 × 10
4 to 9 × 10
2 CFU/mL for yeasts and from 1.8 × 10
3 to 2 × 10
2 CFU/mL for molds in honeys from the Bukovina region (Romania). These levels also exceed those reported by Kędzierska-Matysek M et al.,
in artisanal Polish honeys of different varieties, where counts were below 10 CFU/g, as well as those reported by Bakary C et al.,
| [12] | Bakary C, Masse D, Ibrahim K, Guichard BL (2019) Microbiological quality, physicochemical properties, and sensory profile of honeys from the Worodougou region, Côte d’Ivoire. ESJ 15: https://doi.org/10.19044/esj.2019.v15n30p72 |
[12]
in Côte d’Ivoire (Worodougou region), with a value of 2 CFU/g. These discrepancies may be attributed to variations in environmental conditions (floral origin, climate), hygiene practices during harvesting and handling, and storage conditions.
No anaerobic bacteria were detected in the analyzed samples, which is consistent with the findings of Kędzierska-Matysek M. et al.
, who detected these microorganisms in only 0.1 g of a single buckwheat honey sample among all Polish artisanal honeys studied. The absence of sulfite-reducing anaerobes observed in the present study also agrees with the results of Iurlina MO et al.
, who reported the absence of sulfite-reducing Clostridium spp. in 70 honey samples from Argentina of different botanical origins. Furthermore, these results are consistent with those of Snowdon JA et al.
, who did not detect any vegetative forms of pathogenic bacteria in honey. This absence of anaerobic bacteria may be explained by the intrinsic properties of honey, including low water activity, acidic pH, and the presence of antimicrobial compounds. However, it should be noted that bacterial spores, particularly those of Clostridium spp., may persist in honey without being detected by conventional enumeration methods, highlighting the limitations of detection thresholds and analytical approaches.
No
Salmonella spp were detected in the analyzed samples, which is in agreement with the findings of Vázquez-Quiñones CR et al.
| [3] | Vázquez-Quiñones CR, Moreno-Terrazas R, Natividad-Bonifacio I, et al. (2018) Microbiological assessment of honey in Mexico. Revista Argentina de Microbiología. 50: 75-80.
https://doi.org/10.1016/j.ram.2017.04.005 |
[3]
, who also reported its absence in honey samples studied in Mexico. These observations are consistent with those of Bruxel F et al.
, who did not detect
Salmonella spp. in commercial honeys during food safety and quality control assessments. Similarly, Bakary C et al.
| [12] | Bakary C, Masse D, Ibrahim K, Guichard BL (2019) Microbiological quality, physicochemical properties, and sensory profile of honeys from the Worodougou region, Côte d’Ivoire. ESJ 15: https://doi.org/10.19044/esj.2019.v15n30p72 |
[12]
reported the absence of
Salmonella spp. in all analyzed honey samples, while Iurlina MO et al.,
confirmed that this microorganism was not detecte in Argentine honeys of different botanical origins. The absence of
Salmonella spp. in the studied samples may be explained by the intrinsic properties of honey, particularly low water activity, acidic pH, and antimicrobial compounds that limit the survival of pathogenic bacteria. Nevertheless, this absence should be interpreted with caution, considering the detection limits of analytical methods (generally expressed in CFU/25 g) and the potential risk of post-harvest contamination during handling and storage operations.
5. Conclusion
This study aimed to evaluate the physicochemical and microbiological quality of honey marketed in the urban municipality of Macenta (Guinea). The results showed that, from a physicochemical standpoint, most samples exhibited characteristics generally compliant with honey quality standards, particularly in terms of moisture content, pH, and sugar composition, indicating good preservation potential. However, the observed variations in ash and sugar contents suggest a certain heterogeneity, likely related to differences in botanical origin and production conditions. From a microbiological perspective, most samples showed low counts of total aerobic mesophilic flora and an absence of major pathogenic microorganisms such as Salmonella spp. and sulfite-reducing anaerobes, indicating overall satisfactory quality. Nevertheless, the presence of coliforms and elevated levels of yeasts and molds in some samples reveals deficiencies in hygiene practices during production, handling, and marketing. Overall, although the honey marketed in Macenta is generally compliant in terms of physicochemical quality, its microbiological quality remains heterogeneous. These findings highlight the need to strengthen good hygiene practices, improve storage conditions, and enhance the training of stakeholders in the apiculture sector. Future research should focus on a more detailed characterization of honey, including the identification of botanical origin through melissopalynological analysis, the assessment of chemical contaminants such as pesticide residues and heavy metals, and the evaluation of storage stability over time. In addition, studies investigating the impact of improved processing and handling practices on honey quality, as well as consumer perception and market acceptance, would provide valuable insights for the sustainable development of the honey sector in Guinea.
Author Contributions
Sekou Kouyate: Conceptualization, Funding acquisition, Project administration, Writing – original draft
Ndeye Adiara Ndiaye: Validation, Writing – review & editing
Lahat Niang: Validation, Writing – review & editing
Modou Dieng: Validation, Writing – review & editing
Aly Traore: Data curation, Funding acquisition, Investigation, Methodology