Analytical Method Validation for Quantification of Chloramphenicol Residues in Poultry Meal Using a Liquid Chromatography-Tandem Mass Spectrometry
Md. Zahangir Hosain,
S. M. Shariful Islam,
Md. Mostofa Kamal
Issue:
Volume 6, Issue 2, December 2022
Pages:
44-50
Received:
4 June 2022
Accepted:
20 June 2022
Published:
5 July 2022
DOI:
10.11648/j.ajcbe.20220602.11
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Abstract: Quantification of Chloramphenicol (CAP) residues in complex matrices such as poultry meal is a tedious analytical procedure. In this study, a rapid and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous detection and quantification of CAP residues in poultry meals. The chromatographic separation of the CAP was performed at 40°C column temperature on a reverse-phase C18 column using a binary gradient pump mode and quantification of CAP was performed by LC-MS/MS in electrospray mode. Mobile phase constituents were solvent (a) deionized water, and (b) acetonitrile. The flow rate was 0.35 mL/min and the entire run time was 5 min. The method was validated according to 2021/808/EC guidelines, and acceptance criteria for specificity, linearity, recovery, and precision were met in all the cases. The relative standard deviation (RSD) for precision was < 11% for all the cases. The linearity of the calibration curves was excellent (R2 > 0.999) at concentrations of 0.25, 0.50, 0.75, 1.0, 2.0, and 5.0 µg/kg for matrix-matched CAP standard, and the range of linearity of this method was 0.0-5.0 μg/kg with R2 value greater than 0.99. The decision limit (CCα) and detection capability (CCβ) were 0.29 µg/kg and 32 µg/kg respectively, and the recovery percentages ranged between 94% and 100 %. The obtained results of the proposed method met the validation criteria and this method could be a precise and highly desirable analytical procedure for rapid and accurate quantification of chloramphenicol residues in poultry meal.
Abstract: Quantification of Chloramphenicol (CAP) residues in complex matrices such as poultry meal is a tedious analytical procedure. In this study, a rapid and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous detection and quantification of CAP residues in poultry meals. The chromatog...
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Synthesis and Characterisation of Proteins Profile of “Streptomyces coelicolor” A3 (2) Cultured Within an Inert Solid-State matRix and Submerged Fermentation
Teresa Matoso Manguangua Victor,
Miguel Filho,
Jose Maria Samuco,
Paul Kamps,
Steve Bull,
Jarka Glassey,
Alan Claude Ward
Issue:
Volume 6, Issue 2, December 2022
Pages:
51-67
Received:
11 September 2022
Accepted:
26 September 2022
Published:
18 October 2022
DOI:
10.11648/j.ajcbe.20220602.12
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Abstract: In this study, an inert solid matrix named 2-Vinylpyridine polymer, was produced, and used as micro-bioreactor, with aims to enhance the production of secondary metabolites, synthetized by a soil dwelling microorganism. coelicolor” A3 (2). To mimic the natural environment of microbial growth, parameters such as pores size (11, 22, 43 μm) of the hydrophilic 2-Vinylpyridine inert matrix and the culture medium were tested in a micro-bioreactor, the influence over the proteins synthetized by “S. coelicolor” A3 (2) were investigated. The produced proteins were characterized using Matrix-Assisted Laser Desorption/Ionization-Time-Of-Flight/Mass Spectrometer (MALDI-TOF/MS). As result, differences in protein profiles of submerged growth and 2-Vinylpyridine Polymeric-matrix growth cultures were observed and bands that showed variability were analyzed by mass spectrometry. In conclusion, three proteins present in these bands were revealed, such as Elongation Factor EF-tu which was predominant in submerged growth, while the hypothetical protein SCO7276 and S-adenosylmethionine synthetase were identified in bands detected in protein profile from 2-Vinylpyridine Polymeric-matrix. The enhanced levels of S-adenosylmethionine synthetase are consistent with the elevated levels of secondary metabolite biosynthesis in Streptomyces species.
Abstract: In this study, an inert solid matrix named 2-Vinylpyridine polymer, was produced, and used as micro-bioreactor, with aims to enhance the production of secondary metabolites, synthetized by a soil dwelling microorganism. coelicolor” A3 (2). To mimic the natural environment of microbial growth, parameters such as pores size (11, 22, 43 μm) of the hyd...
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