Quantification of Chloramphenicol (CAP) residues in complex matrices such as poultry meal is a tedious analytical procedure. In this study, a rapid and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous detection and quantification of CAP residues in poultry meals. The chromatographic separation of the CAP was performed at 40°C column temperature on a reverse-phase C18 column using a binary gradient pump mode and quantification of CAP was performed by LC-MS/MS in electrospray mode. Mobile phase constituents were solvent (a) deionized water, and (b) acetonitrile. The flow rate was 0.35 mL/min and the entire run time was 5 min. The method was validated according to 2021/808/EC guidelines, and acceptance criteria for specificity, linearity, recovery, and precision were met in all the cases. The relative standard deviation (RSD) for precision was < 11% for all the cases. The linearity of the calibration curves was excellent (R2 > 0.999) at concentrations of 0.25, 0.50, 0.75, 1.0, 2.0, and 5.0 µg/kg for matrix-matched CAP standard, and the range of linearity of this method was 0.0-5.0 μg/kg with R2 value greater than 0.99. The decision limit (CCα) and detection capability (CCβ) were 0.29 µg/kg and 32 µg/kg respectively, and the recovery percentages ranged between 94% and 100 %. The obtained results of the proposed method met the validation criteria and this method could be a precise and highly desirable analytical procedure for rapid and accurate quantification of chloramphenicol residues in poultry meal.
| Published in | American Journal of Chemical and Biochemical Engineering (Volume 6, Issue 2) |
| DOI | 10.11648/j.ajcbe.20220602.11 |
| Page(s) | 44-50 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Chloramphenicol Residues, Poultry Meal, LC-MS/MS, Method Development & Validation
| [1] | Van, T. T. H., Yidana, Z., Smooker, P. M., & Coloe, P. J. (2020). Antibiotic use in food animals worldwide, with a focus on Africa: Pluses and minuses. Journal of global antimicrobial resistance, 20: 170-177. |
| [2] | Mishra, A., Chhonker, Y. S., Bisen, A. C., Prasad, Y. D., Tulsankar, S. L., Chandasana, H.,... & Bhatta, R. S. (2020). Rapid and Simultaneous Analysis of Multiple Classes of Antimicrobial Drugs by Liquid Chromatography-Tandem Mass Spectrometry and Its Application to Routine Biomedical, Food, and Soil Analyses. ACS omega. |
| [3] | Jung, H. N., Park, D. H., Choi, Y. J., Kang, S. H., Cho, H. J., Choi, J. M.,... & Shin, H. C. (2021). Simultaneous Quantification of Chloramphenicol, Thiamphenicol, Florfenicol, and Florfenicol Amine in Animal and Aquaculture Products Using Liquid Chromatography-Tandem Mass Spectrometry. Frontiers in Nutrition, 8. |
| [4] | Akter Mou, S., Islam, R., Shoeb, M., & Nahar, N. (2021). Determination of chloramphenicol in meat samples using liquid chromatography–tandem mass spectrometry. Food Science & Nutrition, 9 (10): 5670-5675. |
| [5] | Chowdhury, S., Ghosh, S., Aleem, M. A., Parveen, S., Islam, M., Rashid, M.,... & Chowdhury, F. (2021). Antibiotic Usage and Resistance in Food Animal Production: What Have We Learned from Bangladesh?. Antibiotics, 10 (9): 1032. |
| [6] | Xie, X., Wang, B., Pang, M., Zhao, X., Xie, K., Zhang, Y.,... & Wang, J. (2018). Quantitative analysis of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in eggs via liquid chromatography-electrospray ionization tandem mass spectrometry. Food chemistry, 269: 542-548. |
| [7] | Samsonova JV, Cannavan A, Elliott CT (2012) A critical review of screening methods for the detection of chloramphenicol, thiamphenicol, and florfenicol residues in foodstuffs. Critical Reviews in Analytical Chemistry, 42: 50–78. |
| [8] | Lu Y, Shen Q, Dai Z, Zhang H (2010) Multi-walled carbon nanotubes as solid-phase extraction adsorbent for the ultrafast determination of chloramphenicol in egg, honey, and milk by fused-core C18-based high-performance liquid chromatography-tandem mass spectrometry. Analytical and bioanalytical chemistry, 398: 1819–1826. |
| [9] | Martins-Junior HA, Bustillos OV, Pires MAF, Lebre DT, Wang AY (2006). Determination of chloramphenicol residues in industrialized milk and honey samples using LC–MS/MS. Quimica Nova, 29: 586–592. |
| [10] | Bogusz MJ, Hassan H, Al-Enazi E, Ibrahim Z, Al-Tufail M (2004). Rapid determination of chloramphenicol and its glucuronide in food products by liquid chromatography–electrospray negative ionization tandem mass spectrometry. Journal of chromatography B, 807: 343–356. |
| [11] | Forti AF, Campana G, Simonella A, Multari M, Scortichini G (2005). Determination of chloramphenicol in honey by liquid chromatography-tandem mass spectrometry. Analytica chimica acta, 529: 257–263. |
| [12] | Zhang SX, Liu ZW, Guo X, Cheng LL, Wang ZH, Shen JZ (2008) Simultaneous determination and confirmation of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine in chicken muscle by liquid chromatography–tandem mass spectrometry. Journal of chromatography B, 875: 399–404. |
| [13] | Śniegocki, T., Sell, B., Giergiel, M., & Posyniak, A. (2019). QuEChERS and HPLC-MS/MS Combination for the Determination of Chloramphenicol in Twenty-Two Different Matrices. Molecules, 24 (3): 384. |
| [14] | Rřnning HT, Einarsen K, Asp TN (2006). Determination of chloramphenicol residues in meat, seafood, egg, honey, milk, plasma and urine with liquid chromatography–tandem mass spectrometry, and the validation of the method based on 2002/657/EC. Journal of chromatography A, 1118: 226–233. |
| [15] | Pfenning, A. P., Roybal, J. E., Rupp, H. S., Turnipseed, S. B., Gonzales, S. A., & Hurlbut, J. A. (2000). Simultaneous determination of residues of chloramphenicol, florfenicol, florfenicol amine, and thiamphenicol in shrimp tissue by gas chromatography with electron capture detection. Journal of AOAC International, 83 (1): 26-30. |
| [16] | Wu, X., Shen, X., Cao, X., Nie, R., Zhang, H., Tang, C., & Wang, W. (2021). Simultaneous Determination of Amphenicols and Metabolites in Animal-Derived Foods Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry. International Journal of Analytical Chemistry, 2021. |
| [17] | 2021/808/EC. Commission Implementing Regulation (EU) 2021/808 of 22 March 2021 on the performance of analytical methods for residues of pharmacologically active substances used in food-producing animals and on the interpretation of results as well as on the methods to be used for sampling and repealing Decisions 2002/657/EC and 98/179/EC. Offical Journal of the European Union. 2021; L180: 84–109. |
| [18] | Tölgyesi, Á., Fekete, J., Sharma, V. K., Palffi, É., Bekesi, K., Lukonics, D., & Pleva, G. (2014). A LC-MS/MS confirmatory method for determination of chloramphenicol in real samples screened by competitive immunoassay. Acta Alimentaria, 43 (2): 306-314. |
| [19] | Patyra, E., & Kwiatek, K. (2020). Quantification and Analysis of Trace Levels of Phenicols in Feed by Liquid Chromatography–Mass Spectrometry. Chromatographia, 83 (6): 715-723. |
| [20] | Xie KZ, Jia LF, Yao YL, Xu D, Chen SQ, Xie X (2011). Simultaneous determination of thiamphenicol, florfenicol and florfenicol amine in eggs by reversed-phase high performance liquid chromatography with fluorescence detection. Journal of chromatography B 879: 2351–2354. |
| [21] | Pietro, W. J., Woźniak, A., Pasik, K., Cybulski, W., & Krasucka, D. (2014). Amphenicols stability in medicated feed–development and validation of liquid chromatography method. Journal of Veterinary Research, 58 (4): 621-629. |
| [22] | Neuhaus, B. K., Hurlbut, J. A., & Hammack, W. (2002). LC/MS/MS analysis of chloramphenicol in shrimp. Laboratory Information Bulletin, 4290: 1-13. |
| [23] | Patyra, E., & Kwiatek, K. (2017). Determination of fluoroquinolones in animal feed by ion pair high-performance liquid chromatography with fluorescence detection. Analytical Letters, 50 (11): 1711-1720. |
| [24] | Imran, M., Tawab, A., Rauf, W., Rahman, M., Khan, Q. M., Asi, M. R., & Iqbal, M. (2017). LC–MS/MS based method development for the analysis of florfenicol and its application to estimate relative distribution in various tissues of broiler chicken. Journal of Chromatography B, 1063: 163-173. |
| [25] | Kikuchi, H., Sakai, T., Teshima, R., Nemoto, S., & Akiyama, H. (2017). Total determination of chloramphenicol residues in foods by liquid chromatography-tandem mass spectrometry. Food chemistry, 230: 589-593. |
| [26] | Konda, S., Asati, A., Williams, M., & Mudiam, M. K. R. (2022). Development and evaluation of a multi-class analytical method based on solid-phase extraction combined with liquid chromatography-tandem mass spectrometry for the analysis of pharmaceuticals and personal care products in urban wastewater samples. Separation Science Plus, 5 (5): 105-119. |
APA Style
Md. Zahangir Hosain, S. M. Shariful Islam, Md. Mostofa Kamal. (2022). Analytical Method Validation for Quantification of Chloramphenicol Residues in Poultry Meal Using a Liquid Chromatography-Tandem Mass Spectrometry. American Journal of Chemical and Biochemical Engineering, 6(2), 44-50. https://doi.org/10.11648/j.ajcbe.20220602.11
ACS Style
Md. Zahangir Hosain; S. M. Shariful Islam; Md. Mostofa Kamal. Analytical Method Validation for Quantification of Chloramphenicol Residues in Poultry Meal Using a Liquid Chromatography-Tandem Mass Spectrometry. Am. J. Chem. Biochem. Eng. 2022, 6(2), 44-50. doi: 10.11648/j.ajcbe.20220602.11
AMA Style
Md. Zahangir Hosain, S. M. Shariful Islam, Md. Mostofa Kamal. Analytical Method Validation for Quantification of Chloramphenicol Residues in Poultry Meal Using a Liquid Chromatography-Tandem Mass Spectrometry. Am J Chem Biochem Eng. 2022;6(2):44-50. doi: 10.11648/j.ajcbe.20220602.11
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Download@article{10.11648/j.ajcbe.20220602.11,
author = {Md. Zahangir Hosain and S. M. Shariful Islam and Md. Mostofa Kamal},
title = {Analytical Method Validation for Quantification of Chloramphenicol Residues in Poultry Meal Using a Liquid Chromatography-Tandem Mass Spectrometry},
journal = {American Journal of Chemical and Biochemical Engineering},
volume = {6},
number = {2},
pages = {44-50},
doi = {10.11648/j.ajcbe.20220602.11},
url = {https://doi.org/10.11648/j.ajcbe.20220602.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcbe.20220602.11},
abstract = {Quantification of Chloramphenicol (CAP) residues in complex matrices such as poultry meal is a tedious analytical procedure. In this study, a rapid and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous detection and quantification of CAP residues in poultry meals. The chromatographic separation of the CAP was performed at 40°C column temperature on a reverse-phase C18 column using a binary gradient pump mode and quantification of CAP was performed by LC-MS/MS in electrospray mode. Mobile phase constituents were solvent (a) deionized water, and (b) acetonitrile. The flow rate was 0.35 mL/min and the entire run time was 5 min. The method was validated according to 2021/808/EC guidelines, and acceptance criteria for specificity, linearity, recovery, and precision were met in all the cases. The relative standard deviation (RSD) for precision was 2 > 0.999) at concentrations of 0.25, 0.50, 0.75, 1.0, 2.0, and 5.0 µg/kg for matrix-matched CAP standard, and the range of linearity of this method was 0.0-5.0 μg/kg with R2 value greater than 0.99. The decision limit (CCα) and detection capability (CCβ) were 0.29 µg/kg and 32 µg/kg respectively, and the recovery percentages ranged between 94% and 100 %. The obtained results of the proposed method met the validation criteria and this method could be a precise and highly desirable analytical procedure for rapid and accurate quantification of chloramphenicol residues in poultry meal.},
year = {2022}
}
TY - JOUR T1 - Analytical Method Validation for Quantification of Chloramphenicol Residues in Poultry Meal Using a Liquid Chromatography-Tandem Mass Spectrometry AU - Md. Zahangir Hosain AU - S. M. Shariful Islam AU - Md. Mostofa Kamal Y1 - 2022/07/05 PY - 2022 N1 - https://doi.org/10.11648/j.ajcbe.20220602.11 DO - 10.11648/j.ajcbe.20220602.11 T2 - American Journal of Chemical and Biochemical Engineering JF - American Journal of Chemical and Biochemical Engineering JO - American Journal of Chemical and Biochemical Engineering SP - 44 EP - 50 PB - Science Publishing Group SN - 2639-9989 UR - https://doi.org/10.11648/j.ajcbe.20220602.11 AB - Quantification of Chloramphenicol (CAP) residues in complex matrices such as poultry meal is a tedious analytical procedure. In this study, a rapid and precise liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous detection and quantification of CAP residues in poultry meals. The chromatographic separation of the CAP was performed at 40°C column temperature on a reverse-phase C18 column using a binary gradient pump mode and quantification of CAP was performed by LC-MS/MS in electrospray mode. Mobile phase constituents were solvent (a) deionized water, and (b) acetonitrile. The flow rate was 0.35 mL/min and the entire run time was 5 min. The method was validated according to 2021/808/EC guidelines, and acceptance criteria for specificity, linearity, recovery, and precision were met in all the cases. The relative standard deviation (RSD) for precision was 2 > 0.999) at concentrations of 0.25, 0.50, 0.75, 1.0, 2.0, and 5.0 µg/kg for matrix-matched CAP standard, and the range of linearity of this method was 0.0-5.0 μg/kg with R2 value greater than 0.99. The decision limit (CCα) and detection capability (CCβ) were 0.29 µg/kg and 32 µg/kg respectively, and the recovery percentages ranged between 94% and 100 %. The obtained results of the proposed method met the validation criteria and this method could be a precise and highly desirable analytical procedure for rapid and accurate quantification of chloramphenicol residues in poultry meal. VL - 6 IS - 2 ER -
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