Research Article
Anti-Inflammatory Activity of Viscum album D6 Preparations in Eye Drops Formulation
Issue:
Volume 6, Issue 1, March 2025
Pages:
1-6
Received:
17 November 2024
Accepted:
25 December 2024
Published:
16 January 2025
DOI:
10.11648/j.rd.20250601.11
Downloads:
Views:
Abstract: Viscum album is widely recognized in complementary medicine for its antidiabetic, analgesic, anti-inflammatory, and antitumor properties. This plant contains various bioactive compounds, allowing for the observation of diverse pharmacological effects. The present study aimed to evaluate, by in vitro testing, the effect of the Viscum album 6DH eye drops on ocular inflammation using human keratinocytes (HaCaT) and human mesenchymal stem cells (MSCs). HaCaT cells were utilized for the MTT cytotoxicity assay, which determined the optimal concentration of the eye drops (8 µL/mL) for subsequent experiments. In the scratch assay, treatment with the eye drops resulted in significantly enhanced scratch closure compared to the control group. In parallel, MSCs were employed for cytokine analysis. Inflammation was induced using lipopolysaccharides (LPS) prior to treatment with the eye drops. Results indicated a reduction in the pro-inflammatory cytokines IL-8 and IL-12p70 following treatment. These findings suggest that the anti-inflammatory properties of Viscum album can be effectively harnessed in this pharmaceutical form (homeopathic eye drops), offering potential therapeutic applications for the treatment of ocular inflammation.
Abstract: Viscum album is widely recognized in complementary medicine for its antidiabetic, analgesic, anti-inflammatory, and antitumor properties. This plant contains various bioactive compounds, allowing for the observation of diverse pharmacological effects. The present study aimed to evaluate, by in vitro testing, the effect of the Viscum album 6DH eye d...
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Aloísio Cunha de Carvalho
