As a class of common plant hormones, cytokinins (CTKs) can greatly promote cell division and have wide application in agricultural production. Therefore, it is necessary to monitor the residue of CTKs in food or environment. Molecular Imprinting polymer (MIP) with kinetin-6-furfurylaminopurine (6-KT) as the template molecule was prepared using multiple polymerizations. The MIP was used as the fiber coating of solid-phase microextraction (SPME), and then molecular imprinting solid-phase microextraction (MISPME) assay was developed. A series of MISPME extraction conditions were optimized in detail. The extraction was performed at 60°C for 30 min with n-hexane as the extraction solvent and stirring speed of 400 rpm, with 15 µL ethanol as the desorption solvent and desorption for 10 min. For vegetable and environmental water samples, two kinds of CTKs were analyzed by high performance liquid chromatography with UV detector. The enrichment factors of kinetin-6-furfurylaminopurine (6-KT) and N6-benzyladenine (6-BA) were 154 and 163, with whose detection limits of 0.3 ng/mL and 0.5 ng/mL, respectively. The proposed method was sensitive and reproducible, and it was suitable to the pretreatment and selective enrichment of plant hormones.
| Published in | Asia-Pacific Journal of Chemistry (Volume 1, Issue 1) |
| Page(s) | 13-18 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Molecular Imprinting Technique, Solid-Phase Microextraction, Cytokinin, High Performance Liquid Chromatography
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APA Style
Lu Qiaomei, Fan Liangbiao, Zhang Lan. (2019). Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins. Asia-Pacific Journal of Chemistry, 1(1), 13-18.
ACS Style
Lu Qiaomei; Fan Liangbiao; Zhang Lan. Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins. Asia-Pac. J. Chem. 2019, 1(1), 13-18.
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Download@article{10040015,
author = {Lu Qiaomei and Fan Liangbiao and Zhang Lan},
title = {Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins},
journal = {Asia-Pacific Journal of Chemistry},
volume = {1},
number = {1},
pages = {13-18},
url = {https://www.sciencepublishinggroup.com/article/10040015},
abstract = {As a class of common plant hormones, cytokinins (CTKs) can greatly promote cell division and have wide application in agricultural production. Therefore, it is necessary to monitor the residue of CTKs in food or environment. Molecular Imprinting polymer (MIP) with kinetin-6-furfurylaminopurine (6-KT) as the template molecule was prepared using multiple polymerizations. The MIP was used as the fiber coating of solid-phase microextraction (SPME), and then molecular imprinting solid-phase microextraction (MISPME) assay was developed. A series of MISPME extraction conditions were optimized in detail. The extraction was performed at 60°C for 30 min with n-hexane as the extraction solvent and stirring speed of 400 rpm, with 15 µL ethanol as the desorption solvent and desorption for 10 min. For vegetable and environmental water samples, two kinds of CTKs were analyzed by high performance liquid chromatography with UV detector. The enrichment factors of kinetin-6-furfurylaminopurine (6-KT) and N6-benzyladenine (6-BA) were 154 and 163, with whose detection limits of 0.3 ng/mL and 0.5 ng/mL, respectively. The proposed method was sensitive and reproducible, and it was suitable to the pretreatment and selective enrichment of plant hormones.},
year = {2019}
}
TY - JOUR T1 - Preparation and Application of Molecular Imprinting Solid-Phase Microextraction Coating Based on Cytokinins AU - Lu Qiaomei AU - Fan Liangbiao AU - Zhang Lan Y1 - 2019/07/09 PY - 2019 T2 - Asia-Pacific Journal of Chemistry JF - Asia-Pacific Journal of Chemistry JO - Asia-Pacific Journal of Chemistry SP - 13 EP - 18 PB - Science Publishing Group UR - http://www.sciencepg.com/article/10040015 AB - As a class of common plant hormones, cytokinins (CTKs) can greatly promote cell division and have wide application in agricultural production. Therefore, it is necessary to monitor the residue of CTKs in food or environment. Molecular Imprinting polymer (MIP) with kinetin-6-furfurylaminopurine (6-KT) as the template molecule was prepared using multiple polymerizations. The MIP was used as the fiber coating of solid-phase microextraction (SPME), and then molecular imprinting solid-phase microextraction (MISPME) assay was developed. A series of MISPME extraction conditions were optimized in detail. The extraction was performed at 60°C for 30 min with n-hexane as the extraction solvent and stirring speed of 400 rpm, with 15 µL ethanol as the desorption solvent and desorption for 10 min. For vegetable and environmental water samples, two kinds of CTKs were analyzed by high performance liquid chromatography with UV detector. The enrichment factors of kinetin-6-furfurylaminopurine (6-KT) and N6-benzyladenine (6-BA) were 154 and 163, with whose detection limits of 0.3 ng/mL and 0.5 ng/mL, respectively. The proposed method was sensitive and reproducible, and it was suitable to the pretreatment and selective enrichment of plant hormones. VL - 1 IS - 1 ER -
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