Extraction and Comparative Analysis of Berberine Hydrochloride Content from <i>Cortex Phellodendri Chinense</i> and <i>Cortex Phellodendri Amurensis</i>

Pan Huimin; Shu Shiyu; Wang Jiaqi; Ning Chailin; Li Tiechun; Hui Ruihua

doi:doi:

| Peer-Reviewed

Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis

Pan Huimin, Shu Shiyu, Wang Jiaqi, Ning Chailin, Li Tiechun, Hui Ruihua

Published in Asia-Pacific Journal of Chemistry (Volume 1, Issue 1)

Received: 10 April 2019 Accepted: 14 May 2019 Published: 27 May 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Berberine hydrochloride was extracted from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis, and the content of berberine hydrochloride was analyzed and compared. Extraction of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis by ultrasonic method was carried out, and extraction conditions such as extraction reagent concentration, extraction time, extraction temperature and liquid ratio were optimized. The content of berberine hydrochloride in Cortex Phellodendri Chinense and Cortex Phellodendri amurensis was determined by HPLC, and the mobile phase was methanol-acetonitrile-phosphoric acid (0.01 mol/L) (10:28:62,V/V), the flow rate was 1 mL/min, and the detection wavelength was 350 nm. The sample input is 10μL. The linear range is 2.20 mm g/mL ~ 13.20 mm g/mL, the standard deviation (RSD) is less than 2.70 %, and the standard recovery rate is 93.21 ~ 99.38 %. The contents of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis were 57.80mg/g and 48.20 mg/g respectively. The content of berberine hydrochloride in Cortex Phellodendri Chinense was higher than that in Cortex Phellodendri amurensis.

Published in	Asia-Pacific Journal of Chemistry (Volume 1, Issue 1)
Page(s)	8-12
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Cortex Phellodendri Chinense, Cortex Phellodendri Amurensis, Ultrasonic Extraction, Berberine Hydrochloride, HPLC

References

[1]	中华人民共和国国家药典委员会，《中国药典》(一部)[M]. 北京：中国医药科技出版社，2010，137
[2]	吴嘉瑞，张冰，张光敏．黄柏药理作用研究进展[J]．亚太传统医药，2009，5(11)：160—162
[3]	王衡奇，秦民坚，余国奠．黄柏的化学成分及药理学研究进展[J]．中国野生植物资源，2000，2O((4)：6—8
[4]	侯小涛，戴航，周江煜．黄柏的药理研究进展[J]．时珍国医国药，2007，18(2)：498—500
[5]	张义虎,孙静.黄柏的临床应用总述[J] 中国医学创新, 2 01 0,7 ( 3): 1 82一18 3
[6]	俞丹，尹莲．黄柏总生物碱提取工艺的优化研究[J]．海峡药学，2008，20(7)：19-21
[7]	蒋国良，吴珂．黄柏提取工艺的优化fJ]．医药导报，2007，26(7)：792—793
[8]	王楠楠,汪冶,杨建琼.秃叶黄皮树叶子的化学成分研究(英文)[J]天然产物研究与开发, 2010 ,22 (3) : 419-421
[9]	王跃华，苟小军，徐文俊. 川黄柏不同部位中盐酸小檗碱的研究[J], 成都大学学报(自然科学版),2004,23(1):15-17
[10]	程弘夏,雷晓璐,王仲,等. 正交设计法优化黄柏涂膜剂成膜材料的制备工艺[J]．化学与生物工程, 2016,29(8):63-65

Cite This Article

Plain Text BibTeX RIS

APA Style

Pan Huimin, Shu Shiyu, Wang Jiaqi, Ning Chailin, Li Tiechun, et al. (2019). Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis. Asia-Pacific Journal of Chemistry, 1(1), 8-12.

Copy | Download

ACS Style

Pan Huimin; Shu Shiyu; Wang Jiaqi; Ning Chailin; Li Tiechun, et al. Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis. Asia-Pac. J. Chem. 2019, 1(1), 8-12.

Copy | Download

AMA Style

Pan Huimin, Shu Shiyu, Wang Jiaqi, Ning Chailin, Li Tiechun, et al. Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis. Asia-Pac J Chem. 2019;1(1):8-12.

Copy | Download

@article{10038907,
  author = {Pan Huimin and Shu Shiyu and Wang Jiaqi and Ning Chailin and Li Tiechun and Hui Ruihua},
  title = {Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis},
  journal = {Asia-Pacific Journal of Chemistry},
  volume = {1},
  number = {1},
  pages = {8-12},
  url = {https://www.sciencepublishinggroup.com/article/10038907},
  abstract = {Berberine hydrochloride was extracted from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis, and the content of berberine hydrochloride was analyzed and compared.  Extraction of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis by ultrasonic method was carried out, and extraction conditions such as extraction reagent concentration, extraction time, extraction temperature and liquid ratio were optimized. The content of berberine hydrochloride in Cortex Phellodendri Chinense and Cortex Phellodendri amurensis was determined by HPLC, and the mobile phase was methanol-acetonitrile-phosphoric acid (0.01 mol/L) (10:28:62,V/V), the flow rate was 1 mL/min, and the detection wavelength was 350 nm. The sample input is 10μL. The linear range is 2.20 mm g/mL ~ 13.20 mm g/mL, the standard deviation (RSD) is less than 2.70 %, and the standard recovery rate is 93.21 ~ 99.38 %. The contents of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis were 57.80mg/g and 48.20 mg/g respectively. The content of berberine hydrochloride in Cortex Phellodendri Chinense was higher than that in Cortex Phellodendri amurensis.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis
AU  - Pan Huimin
AU  - Shu Shiyu
AU  - Wang Jiaqi
AU  - Ning Chailin
AU  - Li Tiechun
AU  - Hui Ruihua
Y1  - 2019/05/27
PY  - 2019
T2  - Asia-Pacific Journal of Chemistry
JF  - Asia-Pacific Journal of Chemistry
JO  - Asia-Pacific Journal of Chemistry
SP  - 8
EP  - 12
PB  - Science Publishing Group
UR  - http://www.sciencepg.com/article/10038907
AB  - Berberine hydrochloride was extracted from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis, and the content of berberine hydrochloride was analyzed and compared.  Extraction of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis by ultrasonic method was carried out, and extraction conditions such as extraction reagent concentration, extraction time, extraction temperature and liquid ratio were optimized. The content of berberine hydrochloride in Cortex Phellodendri Chinense and Cortex Phellodendri amurensis was determined by HPLC, and the mobile phase was methanol-acetonitrile-phosphoric acid (0.01 mol/L) (10:28:62,V/V), the flow rate was 1 mL/min, and the detection wavelength was 350 nm. The sample input is 10μL. The linear range is 2.20 mm g/mL ~ 13.20 mm g/mL, the standard deviation (RSD) is less than 2.70 %, and the standard recovery rate is 93.21 ~ 99.38 %. The contents of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis were 57.80mg/g and 48.20 mg/g respectively. The content of berberine hydrochloride in Cortex Phellodendri Chinense was higher than that in Cortex Phellodendri amurensis.
VL  - 1
IS  - 1
ER  -

Copy | Download

Author Information

Pan Huimin

College of Chemistry and Life Science, Anshan Normal University, Anshan, China
Shu Shiyu

College of Chemistry and Life Science, Anshan Normal University, Anshan, China
Wang Jiaqi

College of Chemistry and Life Science, Anshan Normal University, Anshan, China
Ning Chailin

College of Chemistry and Life Science, Anshan Normal University, Anshan, China
Li Tiechun

College of Chemistry and Life Science, Anshan Normal University, Anshan, China
Hui Ruihua

College of Chemistry and Life Science, Anshan Normal University, Anshan, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Pan Huimin, Shu Shiyu, Wang Jiaqi, Ning Chailin, Li Tiechun, et al. (2019). Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis. Asia-Pacific Journal of Chemistry, 1(1), 8-12.

Copy | Download

ACS Style

Pan Huimin; Shu Shiyu; Wang Jiaqi; Ning Chailin; Li Tiechun, et al. Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis. Asia-Pac. J. Chem. 2019, 1(1), 8-12.

Copy | Download

AMA Style

Pan Huimin, Shu Shiyu, Wang Jiaqi, Ning Chailin, Li Tiechun, et al. Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis. Asia-Pac J Chem. 2019;1(1):8-12.

Copy | Download

@article{10038907,
  author = {Pan Huimin and Shu Shiyu and Wang Jiaqi and Ning Chailin and Li Tiechun and Hui Ruihua},
  title = {Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis},
  journal = {Asia-Pacific Journal of Chemistry},
  volume = {1},
  number = {1},
  pages = {8-12},
  url = {https://www.sciencepublishinggroup.com/article/10038907},
  abstract = {Berberine hydrochloride was extracted from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis, and the content of berberine hydrochloride was analyzed and compared.  Extraction of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis by ultrasonic method was carried out, and extraction conditions such as extraction reagent concentration, extraction time, extraction temperature and liquid ratio were optimized. The content of berberine hydrochloride in Cortex Phellodendri Chinense and Cortex Phellodendri amurensis was determined by HPLC, and the mobile phase was methanol-acetonitrile-phosphoric acid (0.01 mol/L) (10:28:62,V/V), the flow rate was 1 mL/min, and the detection wavelength was 350 nm. The sample input is 10μL. The linear range is 2.20 mm g/mL ~ 13.20 mm g/mL, the standard deviation (RSD) is less than 2.70 %, and the standard recovery rate is 93.21 ~ 99.38 %. The contents of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis were 57.80mg/g and 48.20 mg/g respectively. The content of berberine hydrochloride in Cortex Phellodendri Chinense was higher than that in Cortex Phellodendri amurensis.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis
AU  - Pan Huimin
AU  - Shu Shiyu
AU  - Wang Jiaqi
AU  - Ning Chailin
AU  - Li Tiechun
AU  - Hui Ruihua
Y1  - 2019/05/27
PY  - 2019
T2  - Asia-Pacific Journal of Chemistry
JF  - Asia-Pacific Journal of Chemistry
JO  - Asia-Pacific Journal of Chemistry
SP  - 8
EP  - 12
PB  - Science Publishing Group
UR  - http://www.sciencepg.com/article/10038907
AB  - Berberine hydrochloride was extracted from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis, and the content of berberine hydrochloride was analyzed and compared.  Extraction of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis by ultrasonic method was carried out, and extraction conditions such as extraction reagent concentration, extraction time, extraction temperature and liquid ratio were optimized. The content of berberine hydrochloride in Cortex Phellodendri Chinense and Cortex Phellodendri amurensis was determined by HPLC, and the mobile phase was methanol-acetonitrile-phosphoric acid (0.01 mol/L) (10:28:62,V/V), the flow rate was 1 mL/min, and the detection wavelength was 350 nm. The sample input is 10μL. The linear range is 2.20 mm g/mL ~ 13.20 mm g/mL, the standard deviation (RSD) is less than 2.70 %, and the standard recovery rate is 93.21 ~ 99.38 %. The contents of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis were 57.80mg/g and 48.20 mg/g respectively. The content of berberine hydrochloride in Cortex Phellodendri Chinense was higher than that in Cortex Phellodendri amurensis.
VL  - 1
IS  - 1
ER  -

Copy | Download