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Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis

Received: 10 April 2019     Accepted: 14 May 2019     Published: 27 May 2019
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Abstract

Berberine hydrochloride was extracted from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis, and the content of berberine hydrochloride was analyzed and compared. Extraction of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis by ultrasonic method was carried out, and extraction conditions such as extraction reagent concentration, extraction time, extraction temperature and liquid ratio were optimized. The content of berberine hydrochloride in Cortex Phellodendri Chinense and Cortex Phellodendri amurensis was determined by HPLC, and the mobile phase was methanol-acetonitrile-phosphoric acid (0.01 mol/L) (10:28:62,V/V), the flow rate was 1 mL/min, and the detection wavelength was 350 nm. The sample input is 10μL. The linear range is 2.20 mm g/mL ~ 13.20 mm g/mL, the standard deviation (RSD) is less than 2.70 %, and the standard recovery rate is 93.21 ~ 99.38 %. The contents of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis were 57.80mg/g and 48.20 mg/g respectively. The content of berberine hydrochloride in Cortex Phellodendri Chinense was higher than that in Cortex Phellodendri amurensis.

Published in Asia-Pacific Journal of Chemistry (Volume 1, Issue 1)
Page(s) 8-12
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Cortex Phellodendri Chinense, Cortex Phellodendri Amurensis, Ultrasonic Extraction, Berberine Hydrochloride, HPLC

References
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[2] 吴嘉瑞,张冰,张光敏.黄柏药理作用研究进展[J].亚太传统医药,2009,5(11):160—162
[3] 王衡奇,秦民坚,余国奠.黄柏的化学成分及药理学研究进展[J].中国野生植物资源,2000,2O((4):6—8
[4] 侯小涛,戴航,周江煜.黄柏的药理研究进展[J].时珍国医国药,2007,18(2):498—500
[5] 张义虎,孙静.黄柏的临床应用总述[J] 中国医学创新, 2 01 0,7 ( 3): 1 82一18 3
[6] 俞丹,尹莲.黄柏总生物碱提取工艺的优化研究[J].海峡药学,2008,20(7):19-21
[7] 蒋国良,吴珂.黄柏提取工艺的优化fJ].医药导报,2007,26(7):792—793
[8] 王楠楠,汪冶,杨建琼.秃叶黄皮树叶子的化学成分研究(英文)[J]天然产物研究与开发, 2010 ,22 (3) : 419-421
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  • APA Style

    Pan Huimin, Shu Shiyu, Wang Jiaqi, Ning Chailin, Li Tiechun, et al. (2019). Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis. Asia-Pacific Journal of Chemistry, 1(1), 8-12.

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    ACS Style

    Pan Huimin; Shu Shiyu; Wang Jiaqi; Ning Chailin; Li Tiechun, et al. Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis. Asia-Pac. J. Chem. 2019, 1(1), 8-12.

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    AMA Style

    Pan Huimin, Shu Shiyu, Wang Jiaqi, Ning Chailin, Li Tiechun, et al. Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis. Asia-Pac J Chem. 2019;1(1):8-12.

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  • @article{10038907,
      author = {Pan Huimin and Shu Shiyu and Wang Jiaqi and Ning Chailin and Li Tiechun and Hui Ruihua},
      title = {Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis},
      journal = {Asia-Pacific Journal of Chemistry},
      volume = {1},
      number = {1},
      pages = {8-12},
      url = {https://www.sciencepublishinggroup.com/article/10038907},
      abstract = {Berberine hydrochloride was extracted from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis, and the content of berberine hydrochloride was analyzed and compared.  Extraction of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis by ultrasonic method was carried out, and extraction conditions such as extraction reagent concentration, extraction time, extraction temperature and liquid ratio were optimized. The content of berberine hydrochloride in Cortex Phellodendri Chinense and Cortex Phellodendri amurensis was determined by HPLC, and the mobile phase was methanol-acetonitrile-phosphoric acid (0.01 mol/L) (10:28:62,V/V), the flow rate was 1 mL/min, and the detection wavelength was 350 nm. The sample input is 10μL. The linear range is 2.20 mm g/mL ~ 13.20 mm g/mL, the standard deviation (RSD) is less than 2.70 %, and the standard recovery rate is 93.21 ~ 99.38 %. The contents of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis were 57.80mg/g and 48.20 mg/g respectively. The content of berberine hydrochloride in Cortex Phellodendri Chinense was higher than that in Cortex Phellodendri amurensis.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Extraction and Comparative Analysis of Berberine Hydrochloride Content from Cortex Phellodendri Chinense and Cortex Phellodendri Amurensis
    AU  - Pan Huimin
    AU  - Shu Shiyu
    AU  - Wang Jiaqi
    AU  - Ning Chailin
    AU  - Li Tiechun
    AU  - Hui Ruihua
    Y1  - 2019/05/27
    PY  - 2019
    T2  - Asia-Pacific Journal of Chemistry
    JF  - Asia-Pacific Journal of Chemistry
    JO  - Asia-Pacific Journal of Chemistry
    SP  - 8
    EP  - 12
    PB  - Science Publishing Group
    UR  - http://www.sciencepg.com/article/10038907
    AB  - Berberine hydrochloride was extracted from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis, and the content of berberine hydrochloride was analyzed and compared.  Extraction of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis by ultrasonic method was carried out, and extraction conditions such as extraction reagent concentration, extraction time, extraction temperature and liquid ratio were optimized. The content of berberine hydrochloride in Cortex Phellodendri Chinense and Cortex Phellodendri amurensis was determined by HPLC, and the mobile phase was methanol-acetonitrile-phosphoric acid (0.01 mol/L) (10:28:62,V/V), the flow rate was 1 mL/min, and the detection wavelength was 350 nm. The sample input is 10μL. The linear range is 2.20 mm g/mL ~ 13.20 mm g/mL, the standard deviation (RSD) is less than 2.70 %, and the standard recovery rate is 93.21 ~ 99.38 %. The contents of berberine hydrochloride from Cortex Phellodendri Chinense and Cortex Phellodendri amurensis were 57.80mg/g and 48.20 mg/g respectively. The content of berberine hydrochloride in Cortex Phellodendri Chinense was higher than that in Cortex Phellodendri amurensis.
    VL  - 1
    IS  - 1
    ER  - 

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Author Information
  • College of Chemistry and Life Science, Anshan Normal University, Anshan, China

  • College of Chemistry and Life Science, Anshan Normal University, Anshan, China

  • College of Chemistry and Life Science, Anshan Normal University, Anshan, China

  • College of Chemistry and Life Science, Anshan Normal University, Anshan, China

  • College of Chemistry and Life Science, Anshan Normal University, Anshan, China

  • College of Chemistry and Life Science, Anshan Normal University, Anshan, China

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