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Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali

Received: 12 September 2023     Accepted: 5 October 2023     Published: 14 October 2023
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Abstract

The sterile male technique (SIT), an autocidal, biological technique that respects the environment, is an efficient tool for tsetse flies eradication. This method requires many sterilized male flies released for mating with wild virgin female flies. The mass production of male flies by rearing in the laboratory and the supply of pupae is becoming more and more difficult. Obtaining sterile males in quality and quantity remains an obstacle to biological control. The aim of this study was to determine the behavior of wild tsetse flies collected in the field for rearing in the insectary to set up a laboratory colony in Mali. Collection was done in the natural habitat nearby the river in the surrounding area of Bamako by using biconical traps set from 06 a.m. to 04 p.m. the following day. Climatic and geographical parameters were recorded. Collected flies have been transferred into a cooler chamber for transportation to the insectary at the Laboratory of Entomology and Parasitology of the Faculty of Sciences and Technics (FST) of the University of Sciences, Technics and Technologies of Bamako (USTTB). Daily monitoring showed that mortality in the 72 hours post-collection varied from 10.4% to 100%. The average survival rate was estimated to 11.28% composed by 6.67% males and 4.61% females. None of them survived after 7 days in the insectary. This fact suggests that the adaptation of wild flies to the insectary needs to be further investigated to make it possible. Meanwhile, the use of pupae to set up a colony or the transfer of flies from another colony seems to be more efficient for the setting of a new colony.

Published in Science Research (Volume 11, Issue 5)
DOI 10.11648/j.sr.20231105.15
Page(s) 123-128
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Wild Tsetse, Rearing, Stress, Adaptation Issue

References
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[3] A. K. Adden, L. R. Haines, lvaro Acosta-Serrano, and L. L. Prieto-Godino,(2023). Tsetse flies (Glossina morsitans morsitans) choose birthing sites guided by substrate cues with no evidence for a role of pheromones,” vol. 290, no. 1997. Royal Society. Published: 26 April 2023; https://doi.org/10.1098/rspb.2023.0030
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[6] Bauer (B.), Ivannek K. H., hamann H. J., adamsky. (1980). 10se of gamma-frradiated blood for feeding tsetse flies. Isotope and radiation research on animal diseases an their vectors Proc. Symp. Vienna, 1979. Vienna, I. A. E. A.: 319.
[7] Bauer B., Wetzel H. (1986). A new membrane for feeding Glossina morsitans Westw. (Diptera, Glossinidae). Bull. ent. Res., 65: 563-565.
[8] Baloguin R. A.. (1977). Maintenance of Glossina palpalis fed through bat's wing membrane on defibrinated blood. Acta too, 34: 321-325.
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  • APA Style

    Traore, A., Koné, O. I., Bass, B., Ly, B., Kounta, C. A., et al. (2023). Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali. Science Research, 11(5), 123-128. https://doi.org/10.11648/j.sr.20231105.15

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    ACS Style

    Traore, A.; Koné, O. I.; Bass, B.; Ly, B.; Kounta, C. A., et al. Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali. Sci. Res. 2023, 11(5), 123-128. doi: 10.11648/j.sr.20231105.15

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    AMA Style

    Traore A, Koné OI, Bass B, Ly B, Kounta CA, et al. Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali. Sci Res. 2023;11(5):123-128. doi: 10.11648/j.sr.20231105.15

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  • @article{10.11648/j.sr.20231105.15,
      author = {Astan Traore and Ousmane Ibrahim Koné and Boubacar Bass and Bintou Ly and Cheick Abou Kounta and Youssouf Faya Kéita and Ambielè Bernard Sodio and Alpha Seydou Yaro},
      title = {Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali},
      journal = {Science Research},
      volume = {11},
      number = {5},
      pages = {123-128},
      doi = {10.11648/j.sr.20231105.15},
      url = {https://doi.org/10.11648/j.sr.20231105.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sr.20231105.15},
      abstract = {The sterile male technique (SIT), an autocidal, biological technique that respects the environment, is an efficient tool for tsetse flies eradication. This method requires many sterilized male flies released for mating with wild virgin female flies. The mass production of male flies by rearing in the laboratory and the supply of pupae is becoming more and more difficult. Obtaining sterile males in quality and quantity remains an obstacle to biological control. The aim of this study was to determine the behavior of wild tsetse flies collected in the field for rearing in the insectary to set up a laboratory colony in Mali. Collection was done in the natural habitat nearby the river in the surrounding area of Bamako by using biconical traps set from 06 a.m. to 04 p.m. the following day. Climatic and geographical parameters were recorded. Collected flies have been transferred into a cooler chamber for transportation to the insectary at the Laboratory of Entomology and Parasitology of the Faculty of Sciences and Technics (FST) of the University of Sciences, Technics and Technologies of Bamako (USTTB). Daily monitoring showed that mortality in the 72 hours post-collection varied from 10.4% to 100%. The average survival rate was estimated to 11.28% composed by 6.67% males and 4.61% females. None of them survived after 7 days in the insectary. This fact suggests that the adaptation of wild flies to the insectary needs to be further investigated to make it possible. Meanwhile, the use of pupae to set up a colony or the transfer of flies from another colony seems to be more efficient for the setting of a new colony.},
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Post Collection Stress of Tsetse Flies Used for the Setting of Lab Colony in Mali
    AU  - Astan Traore
    AU  - Ousmane Ibrahim Koné
    AU  - Boubacar Bass
    AU  - Bintou Ly
    AU  - Cheick Abou Kounta
    AU  - Youssouf Faya Kéita
    AU  - Ambielè Bernard Sodio
    AU  - Alpha Seydou Yaro
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    DO  - 10.11648/j.sr.20231105.15
    T2  - Science Research
    JF  - Science Research
    JO  - Science Research
    SP  - 123
    EP  - 128
    PB  - Science Publishing Group
    SN  - 2329-0927
    UR  - https://doi.org/10.11648/j.sr.20231105.15
    AB  - The sterile male technique (SIT), an autocidal, biological technique that respects the environment, is an efficient tool for tsetse flies eradication. This method requires many sterilized male flies released for mating with wild virgin female flies. The mass production of male flies by rearing in the laboratory and the supply of pupae is becoming more and more difficult. Obtaining sterile males in quality and quantity remains an obstacle to biological control. The aim of this study was to determine the behavior of wild tsetse flies collected in the field for rearing in the insectary to set up a laboratory colony in Mali. Collection was done in the natural habitat nearby the river in the surrounding area of Bamako by using biconical traps set from 06 a.m. to 04 p.m. the following day. Climatic and geographical parameters were recorded. Collected flies have been transferred into a cooler chamber for transportation to the insectary at the Laboratory of Entomology and Parasitology of the Faculty of Sciences and Technics (FST) of the University of Sciences, Technics and Technologies of Bamako (USTTB). Daily monitoring showed that mortality in the 72 hours post-collection varied from 10.4% to 100%. The average survival rate was estimated to 11.28% composed by 6.67% males and 4.61% females. None of them survived after 7 days in the insectary. This fact suggests that the adaptation of wild flies to the insectary needs to be further investigated to make it possible. Meanwhile, the use of pupae to set up a colony or the transfer of flies from another colony seems to be more efficient for the setting of a new colony.
    VL  - 11
    IS  - 5
    ER  - 

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Author Information
  • Laboratory of Entomology-Parasitology, Department of Biology, Faculty of Sciences and Techniques (FST), University of Sciences, Techniques and Technologies of Bamako (USTT-B), Hill of Badalabougou, Bamako, Mali

  • Laboratory of Animal Biology, Department of livestock, Rural Polytechnic Institute / Institute of Training and Applied Research (IPR/IFRA), Katibougou, Mali

  • Central Veterinary Laboratory (LCV), Ministry of Rural Development, Bamako, Mali

  • Laboratory of Entomology-Parasitology, Department of Biology, Faculty of Sciences and Techniques (FST), University of Sciences, Techniques and Technologies of Bamako (USTT-B), Hill of Badalabougou, Bamako, Mali

  • Central Veterinary Laboratory (LCV), Ministry of Rural Development, Bamako, Mali

  • Laboratory of Entomology-Parasitology, Department of Biology, Faculty of Sciences and Techniques (FST), University of Sciences, Techniques and Technologies of Bamako (USTT-B), Hill of Badalabougou, Bamako, Mali

  • Laboratory of Entomology-Parasitology, Department of Biology, Faculty of Sciences and Techniques (FST), University of Sciences, Techniques and Technologies of Bamako (USTT-B), Hill of Badalabougou, Bamako, Mali

  • Laboratory of Entomology-Parasitology, Department of Biology, Faculty of Sciences and Techniques (FST), University of Sciences, Techniques and Technologies of Bamako (USTT-B), Hill of Badalabougou, Bamako, Mali; Malaria Research and Training Center, International Centre for Excellence in Research (ICER-MALI), Bamako, Mali

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