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Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology

Received: Sep. 17, 2023    Accepted: Oct. 31, 2023    Published: Nov. 08, 2023
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Abstract

In order to find out the characteristic bands of different kinds of proteins, this study took soybean protein, vitamin milk, milk and coconut milk as the research objects, and SDS-PAGE gel electrophoresis was used to identify the characteristic bands of the above proteins. The experimental results showed that the polyacrylamide band of soybean protein consisted of: b and c protein bands. The typical protein bands of milk protein were c and d protein bands. The typical protein bands of coconut milk protein were c, d, and e protein bands. The typical protein bands of Vitasoy protein showed b, c, and d three protein bands. The b-band was the characteristic band of soybean protein, with a molecular weight of about 35 kDa. The d-band was the characteristic band of milk protein, with a molecular weight of about 30 kDa. The e-band was the characteristic band of coconut milk protein, with a molecular weight of around 25 kDa. Based on these characteristic bands of proteins, the experimental results also suggested that Vitasoy and coconut milk contain a certain amount of milk. These results indicated that SDS-PAGE gel could be used to find the characteristic bands of different kinds of proteins. Based on these characteristic bands, molecular identification of different types of proteins can be carried out.

DOI 10.11648/j.sd.20231106.11
Published in Science Discovery ( Volume 11, Issue 6, December 2023 )
Page(s) 189-192
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

SDS-PAGE Gel Electrophoresis, Milk, Soybean Protein, Coconut Milk, Protein Characteristic Bands

References
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[3] 张猛. 富含牛奶蛋白的饮料对女性运动后能量摄入的影响 [J]. 基因组学与应用生物学, 2019, 38 (04): 1883-1891.
[4] 林塬, 吴毓炜, 王焱等. 椰浆中椰子蛋白的提取、分离和鉴定 [J]. 热带作物学报, 2021, 42 (04): 1106-1112.
[5] 陈春梅, 王确, 周国强等. 植物蛋白及其产品应用的研究进展 [J]. 现代食品, 2022, 28 (20): 53-62.
[6] 梁华梁, 李珂, 冯策. 中国豆奶市场竞争情况盘点 [J]. 乳品与人类, 2020, No. 110 (01): 26-35.
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[11] Yuan Lin, Yan Wang, Zherong Ji, et al. Isolation, Purification, and Identification of Coconut Protein through SDS-PAGE, HPLC, and MALDI-TOF/TOF-MS [J]. Food Analytical Methods, 2020, 13 (prepublish).
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  • APA Style

    Yushan, Z., Biyi, L., Zhihua, S., Yonggang, L. (2023). Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Science Discovery, 11(6), 189-192. https://doi.org/10.11648/j.sd.20231106.11

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    ACS Style

    Yushan, Z.; Biyi, L.; Zhihua, S.; Yonggang, L. Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Sci. Discov. 2023, 11(6), 189-192. doi: 10.11648/j.sd.20231106.11

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    AMA Style

    Yushan Z, Biyi L, Zhihua S, Yonggang L. Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Sci Discov. 2023;11(6):189-192. doi: 10.11648/j.sd.20231106.11

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  • @article{10.11648/j.sd.20231106.11,
      author = {Zhang Yushan and Li Biyi and Shen Zhihua and Liu Yonggang},
      title = {Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology},
      journal = {Science Discovery},
      volume = {11},
      number = {6},
      pages = {189-192},
      doi = {10.11648/j.sd.20231106.11},
      url = {https://doi.org/10.11648/j.sd.20231106.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.sd.20231106.11},
      abstract = {In order to find out the characteristic bands of different kinds of proteins, this study took soybean protein, vitamin milk, milk and coconut milk as the research objects, and SDS-PAGE gel electrophoresis was used to identify the characteristic bands of the above proteins. The experimental results showed that the polyacrylamide band of soybean protein consisted of: b and c protein bands. The typical protein bands of milk protein were c and d protein bands. The typical protein bands of coconut milk protein were c, d, and e protein bands. The typical protein bands of Vitasoy protein showed b, c, and d three protein bands. The b-band was the characteristic band of soybean protein, with a molecular weight of about 35 kDa. The d-band was the characteristic band of milk protein, with a molecular weight of about 30 kDa. The e-band was the characteristic band of coconut milk protein, with a molecular weight of around 25 kDa. Based on these characteristic bands of proteins, the experimental results also suggested that Vitasoy and coconut milk contain a certain amount of milk. These results indicated that SDS-PAGE gel could be used to find the characteristic bands of different kinds of proteins. Based on these characteristic bands, molecular identification of different types of proteins can be carried out.
    },
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology
    AU  - Zhang Yushan
    AU  - Li Biyi
    AU  - Shen Zhihua
    AU  - Liu Yonggang
    Y1  - 2023/11/08
    PY  - 2023
    N1  - https://doi.org/10.11648/j.sd.20231106.11
    DO  - 10.11648/j.sd.20231106.11
    T2  - Science Discovery
    JF  - Science Discovery
    JO  - Science Discovery
    SP  - 189
    EP  - 192
    PB  - Science Publishing Group
    SN  - 2331-0650
    UR  - https://doi.org/10.11648/j.sd.20231106.11
    AB  - In order to find out the characteristic bands of different kinds of proteins, this study took soybean protein, vitamin milk, milk and coconut milk as the research objects, and SDS-PAGE gel electrophoresis was used to identify the characteristic bands of the above proteins. The experimental results showed that the polyacrylamide band of soybean protein consisted of: b and c protein bands. The typical protein bands of milk protein were c and d protein bands. The typical protein bands of coconut milk protein were c, d, and e protein bands. The typical protein bands of Vitasoy protein showed b, c, and d three protein bands. The b-band was the characteristic band of soybean protein, with a molecular weight of about 35 kDa. The d-band was the characteristic band of milk protein, with a molecular weight of about 30 kDa. The e-band was the characteristic band of coconut milk protein, with a molecular weight of around 25 kDa. Based on these characteristic bands of proteins, the experimental results also suggested that Vitasoy and coconut milk contain a certain amount of milk. These results indicated that SDS-PAGE gel could be used to find the characteristic bands of different kinds of proteins. Based on these characteristic bands, molecular identification of different types of proteins can be carried out.
    
    VL  - 11
    IS  - 6
    ER  - 

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Author Information
  • College of Materials and Food, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan, China

  • College of Materials and Food, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan, China

  • College of Materials and Food, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan, China

  • College of Materials and Food, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan, China

  • Section