Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets

Imad Osman Abu Reid; Malak Elrasheed Mohamed

doi:doi:10.11648/j.ijpc.20190504.12

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Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets

Imad Osman Abu Reid, Malak Elrasheed Mohamed

Published in International Journal of Pharmacy and Chemistry (Volume 5, Issue 4)

Received: 5 April 2019 Accepted: 22 July 2019 Published: 9 October 2019

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Abstract

A simple, accurate and precise UV-spectrophotometric method based inverse least-squares was developed for the simultaneous determination of atorvastatin and amlodipine in tablet formulation. The absorbance values of the two analytes were linear with the concentration at the wavelengths taken at 5 nm interval over the range of 230 -260 nm. The calibration equations were developed using the absorbance values of nine synthetic mixtures containing different concentrations of two analytes measured at 5 nm intervalsin the range of 230 -260 nm. The developed equations werethen validated by calculating the analytes recovery from the analysis of a set of another five synthetic mixtures, the mean% recoveries were 100.02% and 100.06% with the corresponding% RSD of ±0.36 and±0.51 for atorvastatin and amlodipine, respectively. The calibration equations obtained were then used to obtain the concentration of each analyte in commercial samples. The mean % recoveries were 100.43% and 100.28% with the corresponding% RSD of ±0.78 and±0.85 for atorvastatin and amlodipine, respectively. The validity of the proposed method was confirmed through the statistical comparison of the obtained results with those obtained by a reference method utilizing high performance liquid chromatography for the determination of the two actives, the calculated t-values at (P=0.05, n =6) were 1.47 and 0.73 compared to the tabulated value of 2.23.

Published in	International Journal of Pharmacy and Chemistry (Volume 5, Issue 4)
DOI	10.11648/j.ijpc.20190504.12
Page(s)	42-47
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Chemometric, Determination, Atorvastatin, Amlodipine

References

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APA Style

Imad Osman Abu Reid, Malak Elrasheed Mohamed. (2019). Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets. International Journal of Pharmacy and Chemistry, 5(4), 42-47. https://doi.org/10.11648/j.ijpc.20190504.12

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ACS Style

Imad Osman Abu Reid; Malak Elrasheed Mohamed. Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets. Int. J. Pharm. Chem. 2019, 5(4), 42-47. doi: 10.11648/j.ijpc.20190504.12

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AMA Style

Imad Osman Abu Reid, Malak Elrasheed Mohamed. Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets. Int J Pharm Chem. 2019;5(4):42-47. doi: 10.11648/j.ijpc.20190504.12

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@article{10.11648/j.ijpc.20190504.12,
  author = {Imad Osman Abu Reid and Malak Elrasheed Mohamed},
  title = {Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {5},
  number = {4},
  pages = {42-47},
  doi = {10.11648/j.ijpc.20190504.12},
  url = {https://doi.org/10.11648/j.ijpc.20190504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20190504.12},
  abstract = {A simple, accurate and precise UV-spectrophotometric method based inverse least-squares was developed for the simultaneous determination of atorvastatin and amlodipine in tablet formulation. The absorbance values of the two analytes were linear with the concentration at the wavelengths taken at 5 nm interval over the range of 230 -260 nm. The calibration equations were developed using the absorbance values of nine synthetic mixtures containing different concentrations of two analytes measured at 5 nm intervalsin the range of 230 -260 nm. The developed equations werethen validated by calculating the analytes recovery from the analysis of a set of another five synthetic mixtures, the mean% recoveries were 100.02% and 100.06% with the corresponding% RSD of ±0.36 and±0.51 for atorvastatin and amlodipine, respectively. The calibration equations obtained were then used to obtain the concentration of each analyte in commercial samples. The mean % recoveries were 100.43% and 100.28% with the corresponding% RSD of ±0.78 and±0.85 for atorvastatin and amlodipine, respectively. The validity of the proposed method was confirmed through the statistical comparison of the obtained results with those obtained by a reference method utilizing high performance liquid chromatography for the determination of the two actives, the calculated t-values at (P=0.05, n =6) were 1.47 and 0.73 compared to the tabulated value of 2.23.},
 year = {2019}
}

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TY  - JOUR
T1  - Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets
AU  - Imad Osman Abu Reid
AU  - Malak Elrasheed Mohamed
Y1  - 2019/10/09
PY  - 2019
N1  - https://doi.org/10.11648/j.ijpc.20190504.12
DO  - 10.11648/j.ijpc.20190504.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 42
EP  - 47
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20190504.12
AB  - A simple, accurate and precise UV-spectrophotometric method based inverse least-squares was developed for the simultaneous determination of atorvastatin and amlodipine in tablet formulation. The absorbance values of the two analytes were linear with the concentration at the wavelengths taken at 5 nm interval over the range of 230 -260 nm. The calibration equations were developed using the absorbance values of nine synthetic mixtures containing different concentrations of two analytes measured at 5 nm intervalsin the range of 230 -260 nm. The developed equations werethen validated by calculating the analytes recovery from the analysis of a set of another five synthetic mixtures, the mean% recoveries were 100.02% and 100.06% with the corresponding% RSD of ±0.36 and±0.51 for atorvastatin and amlodipine, respectively. The calibration equations obtained were then used to obtain the concentration of each analyte in commercial samples. The mean % recoveries were 100.43% and 100.28% with the corresponding% RSD of ±0.78 and±0.85 for atorvastatin and amlodipine, respectively. The validity of the proposed method was confirmed through the statistical comparison of the obtained results with those obtained by a reference method utilizing high performance liquid chromatography for the determination of the two actives, the calculated t-values at (P=0.05, n =6) were 1.47 and 0.73 compared to the tabulated value of 2.23.
VL  - 5
IS  - 4
ER  -

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Author Information

Imad Osman Abu Reid

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National Al Ribat University, Khartoum, Sudan
Malak Elrasheed Mohamed

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National Al Ribat University, Khartoum, Sudan

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APA Style

Imad Osman Abu Reid, Malak Elrasheed Mohamed. (2019). Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets. International Journal of Pharmacy and Chemistry, 5(4), 42-47. https://doi.org/10.11648/j.ijpc.20190504.12

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ACS Style

Imad Osman Abu Reid; Malak Elrasheed Mohamed. Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets. Int. J. Pharm. Chem. 2019, 5(4), 42-47. doi: 10.11648/j.ijpc.20190504.12

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AMA Style

Imad Osman Abu Reid, Malak Elrasheed Mohamed. Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets. Int J Pharm Chem. 2019;5(4):42-47. doi: 10.11648/j.ijpc.20190504.12

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@article{10.11648/j.ijpc.20190504.12,
  author = {Imad Osman Abu Reid and Malak Elrasheed Mohamed},
  title = {Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets},
  journal = {International Journal of Pharmacy and Chemistry},
  volume = {5},
  number = {4},
  pages = {42-47},
  doi = {10.11648/j.ijpc.20190504.12},
  url = {https://doi.org/10.11648/j.ijpc.20190504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20190504.12},
  abstract = {A simple, accurate and precise UV-spectrophotometric method based inverse least-squares was developed for the simultaneous determination of atorvastatin and amlodipine in tablet formulation. The absorbance values of the two analytes were linear with the concentration at the wavelengths taken at 5 nm interval over the range of 230 -260 nm. The calibration equations were developed using the absorbance values of nine synthetic mixtures containing different concentrations of two analytes measured at 5 nm intervalsin the range of 230 -260 nm. The developed equations werethen validated by calculating the analytes recovery from the analysis of a set of another five synthetic mixtures, the mean% recoveries were 100.02% and 100.06% with the corresponding% RSD of ±0.36 and±0.51 for atorvastatin and amlodipine, respectively. The calibration equations obtained were then used to obtain the concentration of each analyte in commercial samples. The mean % recoveries were 100.43% and 100.28% with the corresponding% RSD of ±0.78 and±0.85 for atorvastatin and amlodipine, respectively. The validity of the proposed method was confirmed through the statistical comparison of the obtained results with those obtained by a reference method utilizing high performance liquid chromatography for the determination of the two actives, the calculated t-values at (P=0.05, n =6) were 1.47 and 0.73 compared to the tabulated value of 2.23.},
 year = {2019}
}

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TY  - JOUR
T1  - Chemometric Simultaneous Determination of Atorvastatin and Amlodipine in Bulk and Tablets
AU  - Imad Osman Abu Reid
AU  - Malak Elrasheed Mohamed
Y1  - 2019/10/09
PY  - 2019
N1  - https://doi.org/10.11648/j.ijpc.20190504.12
DO  - 10.11648/j.ijpc.20190504.12
T2  - International Journal of Pharmacy and Chemistry
JF  - International Journal of Pharmacy and Chemistry
JO  - International Journal of Pharmacy and Chemistry
SP  - 42
EP  - 47
PB  - Science Publishing Group
SN  - 2575-5749
UR  - https://doi.org/10.11648/j.ijpc.20190504.12
AB  - A simple, accurate and precise UV-spectrophotometric method based inverse least-squares was developed for the simultaneous determination of atorvastatin and amlodipine in tablet formulation. The absorbance values of the two analytes were linear with the concentration at the wavelengths taken at 5 nm interval over the range of 230 -260 nm. The calibration equations were developed using the absorbance values of nine synthetic mixtures containing different concentrations of two analytes measured at 5 nm intervalsin the range of 230 -260 nm. The developed equations werethen validated by calculating the analytes recovery from the analysis of a set of another five synthetic mixtures, the mean% recoveries were 100.02% and 100.06% with the corresponding% RSD of ±0.36 and±0.51 for atorvastatin and amlodipine, respectively. The calibration equations obtained were then used to obtain the concentration of each analyte in commercial samples. The mean % recoveries were 100.43% and 100.28% with the corresponding% RSD of ±0.78 and±0.85 for atorvastatin and amlodipine, respectively. The validity of the proposed method was confirmed through the statistical comparison of the obtained results with those obtained by a reference method utilizing high performance liquid chromatography for the determination of the two actives, the calculated t-values at (P=0.05, n =6) were 1.47 and 0.73 compared to the tabulated value of 2.23.
VL  - 5
IS  - 4
ER  -

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