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Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination

Received: 14 May 2020     Accepted: 29 May 2020     Published: 23 June 2020
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Abstract

Acrylamide, exists in carbohydrate-rich food heated at high temperatures, is a probable carcinogen to humans. Excessive alcohol intake can also lead to a variety of pathological changes in the body. Acrylamide in the foods and alcohol in the drinks are unavoidable. And studies have demonstrated that combination of the two substances which are taken into the body via diet may cause adverse effects in the cells, even induce impairments on testicular spermatogenesis in male offsprings. Both acrylamide and alcohol are mainly metabolized in the liver, where cytochrome P450 2E1 (CYP2E1) acts as the common metabolic enzyme of the two xenobiotics. This study aimed to explore the effects of acrylamide and alcohol combination on rat hepatoma BRL cells proliferation and the probable mechanisms. MTT, western blotting, EdU fluorescence staining, flow cytometry and PCR were used. Results showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through CYP2E1/Akt/ NF-κB/cyclinB1/cyclinD1 activation and ROS production. The combined effects of acrylamide and alcohol largely depended on ROS level. Furthermore, acrylamide and alcohol could synergistically induce miR-21, which was related to the progress of liver regeneration. These data showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through inducing ROS production, which indicated that intake of fried starch food with small dose of alcohol might have positive effects on liver regeneration.

Published in International Journal of Nutrition and Food Sciences (Volume 9, Issue 3)
DOI 10.11648/j.ijnfs.20200903.12
Page(s) 78-85
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Acrylamide, Alcohol, Proliferation, ROS, miR-21

References
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[8] BRIEN, S. E., RONKSLEY, P. E., TURNER, B. J., MUKAMAL, K. J., GHALI, W. A. (2011). Effect of alcohol consumption on biological markers associated with risk of coronary heart disease: systematic review and meta-analysis of interventional studies. Bmj, 342: 480-480.
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[14] MARQUEZ, R. T., WENDLANDT, E., GALLE, C. S., KECK, K., MCCAFFREY, A. P. (2010). MicroRNA-21 is upregulated during the proliferative phase of liver regeneration, targets Pellino-1, and inhibits NF-kappaB signaling. American Journal of Physiology Gastrointestinal & Liver Physiology, 298: 535-41.
[15] NG, R., SONG, G., ROLL, G. R. (2012). A microRNA-21 surge facilitates rapid cyclin D1 translation and cell cycle progression in mouse liver regeneration. Journal of Clinical Investigation, 122: 1097-108.
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[20] DAY, R. M., SUZUKI, Y. J. (2004). Cell proliferation, reactive oxygen and cellular glutathione. Dose Response, 3: 425-442.
[21] CARRERAS, M. C., CONVERSO, D. P., LORENTI, A. S., BARBICH, M., LEVISMAN, D. M., JAITOVICH, A., ANTICO ARCIUCH, V. G., GALLI, S., PODEROSO, J. J. (2004). Mitochondrial nitric oxide synthase drives redox signals for proliferation and quiescence in rat liver development. Hepatology, 40: 157–166.
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Cite This Article
  • APA Style

    Xiaoyun Shan, Pengqi Wang, Qing Feng. (2020). Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination. International Journal of Nutrition and Food Sciences, 9(3), 78-85. https://doi.org/10.11648/j.ijnfs.20200903.12

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    ACS Style

    Xiaoyun Shan; Pengqi Wang; Qing Feng. Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination. Int. J. Nutr. Food Sci. 2020, 9(3), 78-85. doi: 10.11648/j.ijnfs.20200903.12

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    AMA Style

    Xiaoyun Shan, Pengqi Wang, Qing Feng. Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination. Int J Nutr Food Sci. 2020;9(3):78-85. doi: 10.11648/j.ijnfs.20200903.12

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  • @article{10.11648/j.ijnfs.20200903.12,
      author = {Xiaoyun Shan and Pengqi Wang and Qing Feng},
      title = {Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {9},
      number = {3},
      pages = {78-85},
      doi = {10.11648/j.ijnfs.20200903.12},
      url = {https://doi.org/10.11648/j.ijnfs.20200903.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20200903.12},
      abstract = {Acrylamide, exists in carbohydrate-rich food heated at high temperatures, is a probable carcinogen to humans. Excessive alcohol intake can also lead to a variety of pathological changes in the body. Acrylamide in the foods and alcohol in the drinks are unavoidable. And studies have demonstrated that combination of the two substances which are taken into the body via diet may cause adverse effects in the cells, even induce impairments on testicular spermatogenesis in male offsprings. Both acrylamide and alcohol are mainly metabolized in the liver, where cytochrome P450 2E1 (CYP2E1) acts as the common metabolic enzyme of the two xenobiotics. This study aimed to explore the effects of acrylamide and alcohol combination on rat hepatoma BRL cells proliferation and the probable mechanisms. MTT, western blotting, EdU fluorescence staining, flow cytometry and PCR were used. Results showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through CYP2E1/Akt/ NF-κB/cyclinB1/cyclinD1 activation and ROS production. The combined effects of acrylamide and alcohol largely depended on ROS level. Furthermore, acrylamide and alcohol could synergistically induce miR-21, which was related to the progress of liver regeneration. These data showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through inducing ROS production, which indicated that intake of fried starch food with small dose of alcohol might have positive effects on liver regeneration.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Rat Hepatoma BRL Cells Proliferation by Acrylamide and Alcohol Combination
    AU  - Xiaoyun Shan
    AU  - Pengqi Wang
    AU  - Qing Feng
    Y1  - 2020/06/23
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijnfs.20200903.12
    DO  - 10.11648/j.ijnfs.20200903.12
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 78
    EP  - 85
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20200903.12
    AB  - Acrylamide, exists in carbohydrate-rich food heated at high temperatures, is a probable carcinogen to humans. Excessive alcohol intake can also lead to a variety of pathological changes in the body. Acrylamide in the foods and alcohol in the drinks are unavoidable. And studies have demonstrated that combination of the two substances which are taken into the body via diet may cause adverse effects in the cells, even induce impairments on testicular spermatogenesis in male offsprings. Both acrylamide and alcohol are mainly metabolized in the liver, where cytochrome P450 2E1 (CYP2E1) acts as the common metabolic enzyme of the two xenobiotics. This study aimed to explore the effects of acrylamide and alcohol combination on rat hepatoma BRL cells proliferation and the probable mechanisms. MTT, western blotting, EdU fluorescence staining, flow cytometry and PCR were used. Results showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through CYP2E1/Akt/ NF-κB/cyclinB1/cyclinD1 activation and ROS production. The combined effects of acrylamide and alcohol largely depended on ROS level. Furthermore, acrylamide and alcohol could synergistically induce miR-21, which was related to the progress of liver regeneration. These data showed that combination of acrylamide and alcohol at low doses promoted BRL cells proliferation through inducing ROS production, which indicated that intake of fried starch food with small dose of alcohol might have positive effects on liver regeneration.
    VL  - 9
    IS  - 3
    ER  - 

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Author Information
  • School of Public Health, University of South China, Hengyang, China

  • Lianyungang Health Inspection Bureau, Lianyungang, China

  • Department of Nutrition and Food Hygiene, Nanjing Medical University, Nanjing, China

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