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Flow Cytometric Assessment of Lactococcus Lactis Isolates Viability after Lyophilization

Received: 11 January 2014     Accepted: 20 August 2014     Published: 30 August 2014
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Abstract

Lactococcus lactis strains tolerance to the lyophilization process is one of the criteria used during the selection of starter cultures in cheese production. The viability of L. lactis strains isolated from silage and milk after the lyophilization process was evaluated in the present study by flow cytometry and cell plate counting. The LIVE/DEAD BacLightTM kit, which contains the dyes SYTO 9 and propidium iodide, was used to stain the cells for flow cytometry analysis. The dried cells were also plated on M17 agar and incubated at 30 °C for 24 hours. The strain L. lactis LBU.1, isolated from buffalo milk, showed the highest tolerance to the lyophilization process, according to flow cytometry and cell plate counting. Although the strains of L. lactis isolated from silage were not the most resistant to the lyophilization process, it is noteworthy that, compared to strains isolated from conventional sources such as cow and goat milk, L. lactis strains from silage showed higher tolerance to the lyophilization process.

Published in International Journal of Nutrition and Food Sciences (Volume 3, Issue 5)
DOI 10.11648/j.ijnfs.20140305.15
Page(s) 391-396
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Lactococcus Lactis, Lyophilization, Viability, Flow Cytometry

References
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    Eliana dos Santos Leandro, Graciela Kunrath Lima, Antônio Fernandes de Carvalho, Odilon Gomes Pereira, Célia Alencar de Moraes. (2014). Flow Cytometric Assessment of Lactococcus Lactis Isolates Viability after Lyophilization. International Journal of Nutrition and Food Sciences, 3(5), 391-396. https://doi.org/10.11648/j.ijnfs.20140305.15

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    ACS Style

    Eliana dos Santos Leandro; Graciela Kunrath Lima; Antônio Fernandes de Carvalho; Odilon Gomes Pereira; Célia Alencar de Moraes. Flow Cytometric Assessment of Lactococcus Lactis Isolates Viability after Lyophilization. Int. J. Nutr. Food Sci. 2014, 3(5), 391-396. doi: 10.11648/j.ijnfs.20140305.15

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    AMA Style

    Eliana dos Santos Leandro, Graciela Kunrath Lima, Antônio Fernandes de Carvalho, Odilon Gomes Pereira, Célia Alencar de Moraes. Flow Cytometric Assessment of Lactococcus Lactis Isolates Viability after Lyophilization. Int J Nutr Food Sci. 2014;3(5):391-396. doi: 10.11648/j.ijnfs.20140305.15

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  • @article{10.11648/j.ijnfs.20140305.15,
      author = {Eliana dos Santos Leandro and Graciela Kunrath Lima and Antônio Fernandes de Carvalho and Odilon Gomes Pereira and Célia Alencar de Moraes},
      title = {Flow Cytometric Assessment of Lactococcus Lactis Isolates Viability after Lyophilization},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {3},
      number = {5},
      pages = {391-396},
      doi = {10.11648/j.ijnfs.20140305.15},
      url = {https://doi.org/10.11648/j.ijnfs.20140305.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140305.15},
      abstract = {Lactococcus lactis strains tolerance to the lyophilization process is one of the criteria used during the selection of starter cultures in cheese production. The viability of L. lactis strains isolated from silage and milk after the lyophilization process was evaluated in the present study by flow cytometry and cell plate counting. The LIVE/DEAD BacLightTM kit, which contains the dyes SYTO 9 and propidium iodide, was used to stain the cells for flow cytometry analysis. The dried cells were also plated on M17 agar and incubated at 30 °C for 24 hours. The strain L. lactis LBU.1, isolated from buffalo milk, showed the highest tolerance to the lyophilization process, according to flow cytometry and cell plate counting. Although the strains of L. lactis isolated from silage were not the most resistant to the lyophilization process, it is noteworthy that, compared to strains isolated from conventional sources such as cow and goat milk, L. lactis strains from silage showed higher tolerance to the lyophilization process.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Flow Cytometric Assessment of Lactococcus Lactis Isolates Viability after Lyophilization
    AU  - Eliana dos Santos Leandro
    AU  - Graciela Kunrath Lima
    AU  - Antônio Fernandes de Carvalho
    AU  - Odilon Gomes Pereira
    AU  - Célia Alencar de Moraes
    Y1  - 2014/08/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ijnfs.20140305.15
    DO  - 10.11648/j.ijnfs.20140305.15
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 391
    EP  - 396
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20140305.15
    AB  - Lactococcus lactis strains tolerance to the lyophilization process is one of the criteria used during the selection of starter cultures in cheese production. The viability of L. lactis strains isolated from silage and milk after the lyophilization process was evaluated in the present study by flow cytometry and cell plate counting. The LIVE/DEAD BacLightTM kit, which contains the dyes SYTO 9 and propidium iodide, was used to stain the cells for flow cytometry analysis. The dried cells were also plated on M17 agar and incubated at 30 °C for 24 hours. The strain L. lactis LBU.1, isolated from buffalo milk, showed the highest tolerance to the lyophilization process, according to flow cytometry and cell plate counting. Although the strains of L. lactis isolated from silage were not the most resistant to the lyophilization process, it is noteworthy that, compared to strains isolated from conventional sources such as cow and goat milk, L. lactis strains from silage showed higher tolerance to the lyophilization process.
    VL  - 3
    IS  - 5
    ER  - 

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Author Information
  • Departamento de Microbiologia, Universidade Federal de Vi?osa, Vi?osa, Brazil

  • Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

  • Departamento de Zootecnia, Universidade Federal de Vi?osa, Vi?osa, Brazil

  • Departamento de Microbiologia, Universidade Federal de Vi?osa, Vi?osa, Brazil

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