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Hypolipidaemic Effect of Ethanol Leaf Extract of Moringa Oleifera Lam. in Experimentally induced Hypercholesterolemic Wistar Rats

Received: 16 July 2014     Accepted: 9 August 2014     Published: 20 August 2014
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Abstract

The hypolipidaemic effect of ethanol leaf extract of Moringa oleifera in experimentally induced hypercholesterolemic rats was investigated. Thirty six (36) wistar rats of both sexes weighing 130.53±4.86 were used for the study. The animals were completely randomized into six groups (A-F) comprising 6 animals each. Groups A, B and C comprise female rats administered 1 ml of distilled water, high dose of 600 mg/kg and low dose of 300 mg/kg body weight of the extract respectively. Groups D, E and F comprise male rats administered 1 ml of distilled water, high dose of 600 mg/kg and low dose of 300 mg/kg body weight of the extract respectively. Hypercholesterolemia was induced by feeding the animals with high fat diet for 21 days before administration of the extract. After the 21 days of feeding, administration of extract lasted for 14 days. Preliminary phytochemical screening revealed that the ethanol leaf extract of M. oleifera contains alkaloids, tannins, carbohydrates and cardiac glycosides. Only the high dose female group (600 mg/kg body weight) lost or maintained their body weight significantly (p<0.05), the rest did not. Body weight was not significantly (p>0.05) altered in the male group administered low dose and high dose, showing that the dose of the extract slightly affected their weight. For serum lipids, serum total cholesterol concentration in both male and female reduced significantly (p<0.05), both in those given low and high doses of the extract. Serum high density lipoprotein cholesterol (HDLC) level significantly (p<0.05) increased both in male and female rats that were administered high dose of 600 mg/kg body weight of the extract, but was not significantly (p>0.05) affected in other groups. Serum low density lipoprotein cholesterol (LDLC) level also reduced significantly (p<0.05) in both male and female rats that were administered high dose of the extract, but was not significantly (p>0.05) altered for those that received low doses (300 mg/kg body weight) of the extract. There was no significant (p>0.05) reduction in the LDLC of the male rats. Serum triacylglycerol (TAG) concentration in male and female rats reduced significantly (p<0.05), in those that received low and high doses of the extract. Overall, findings from the present study suggest that the ethanol leaf extract of M. oleifera has hypolipidaemic effect. Therefore, the leafy vegetable may be recommended to patients that have problems with high serum lipid profiles and also for people that want to lose or maintain body weight.

Published in International Journal of Nutrition and Food Sciences (Volume 3, Issue 4)
DOI 10.11648/j.ijnfs.20140304.28
Page(s) 355-360
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Moringa Oleifera, Body Weight, High Fat Diet, Hypercholesterolemia, Serum Lipid, Hypolipidaemic Effect

References
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    Denen Atsukwei, Ejike Daniel Eze, Moses Dele Adams, Seriki Samuel Adinoyi, Chiamaka Nnenna Ukpabi. (2014). Hypolipidaemic Effect of Ethanol Leaf Extract of Moringa Oleifera Lam. in Experimentally induced Hypercholesterolemic Wistar Rats. International Journal of Nutrition and Food Sciences, 3(4), 355-360. https://doi.org/10.11648/j.ijnfs.20140304.28

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    Denen Atsukwei; Ejike Daniel Eze; Moses Dele Adams; Seriki Samuel Adinoyi; Chiamaka Nnenna Ukpabi. Hypolipidaemic Effect of Ethanol Leaf Extract of Moringa Oleifera Lam. in Experimentally induced Hypercholesterolemic Wistar Rats. Int. J. Nutr. Food Sci. 2014, 3(4), 355-360. doi: 10.11648/j.ijnfs.20140304.28

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    AMA Style

    Denen Atsukwei, Ejike Daniel Eze, Moses Dele Adams, Seriki Samuel Adinoyi, Chiamaka Nnenna Ukpabi. Hypolipidaemic Effect of Ethanol Leaf Extract of Moringa Oleifera Lam. in Experimentally induced Hypercholesterolemic Wistar Rats. Int J Nutr Food Sci. 2014;3(4):355-360. doi: 10.11648/j.ijnfs.20140304.28

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  • @article{10.11648/j.ijnfs.20140304.28,
      author = {Denen Atsukwei and Ejike Daniel Eze and Moses Dele Adams and Seriki Samuel Adinoyi and Chiamaka Nnenna Ukpabi},
      title = {Hypolipidaemic Effect of Ethanol Leaf Extract of Moringa Oleifera Lam. in Experimentally induced Hypercholesterolemic Wistar Rats},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {3},
      number = {4},
      pages = {355-360},
      doi = {10.11648/j.ijnfs.20140304.28},
      url = {https://doi.org/10.11648/j.ijnfs.20140304.28},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140304.28},
      abstract = {The hypolipidaemic effect of ethanol leaf extract of Moringa oleifera in experimentally induced hypercholesterolemic rats was investigated. Thirty six (36) wistar rats of both sexes weighing 130.53±4.86 were used for the study. The animals were completely randomized into six groups (A-F) comprising 6 animals each. Groups A, B and C comprise female rats administered 1 ml of distilled water, high dose of 600 mg/kg and low dose of 300 mg/kg body weight of the extract respectively. Groups D, E and F comprise male rats administered 1 ml of distilled water, high dose of 600 mg/kg and low dose of 300 mg/kg body weight of the extract respectively. Hypercholesterolemia was induced by feeding the animals with high fat diet for 21 days before administration of the extract. After the 21 days of feeding, administration of extract lasted for 14 days. Preliminary phytochemical screening revealed that the ethanol leaf extract of M. oleifera contains alkaloids, tannins, carbohydrates and cardiac glycosides. Only the high dose female group (600 mg/kg body weight) lost or maintained their body weight significantly (p0.05) altered in the male group administered low dose and high dose, showing that the dose of the extract slightly affected their weight. For serum lipids, serum total cholesterol concentration in both male and female reduced significantly (p0.05) affected in other groups. Serum low density lipoprotein cholesterol (LDLC) level also reduced significantly (p0.05) altered for those that received low doses (300 mg/kg body weight) of the extract. There was no significant (p>0.05) reduction in the LDLC of the male rats. Serum triacylglycerol (TAG) concentration in male and female rats reduced significantly (p<0.05), in those that received low and high doses of the extract. Overall, findings from the present study suggest that the ethanol leaf extract of M. oleifera has hypolipidaemic effect. Therefore, the leafy vegetable may be recommended to patients that have problems with high serum lipid profiles and also for people that want to lose or maintain body weight.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Hypolipidaemic Effect of Ethanol Leaf Extract of Moringa Oleifera Lam. in Experimentally induced Hypercholesterolemic Wistar Rats
    AU  - Denen Atsukwei
    AU  - Ejike Daniel Eze
    AU  - Moses Dele Adams
    AU  - Seriki Samuel Adinoyi
    AU  - Chiamaka Nnenna Ukpabi
    Y1  - 2014/08/20
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ijnfs.20140304.28
    DO  - 10.11648/j.ijnfs.20140304.28
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 355
    EP  - 360
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20140304.28
    AB  - The hypolipidaemic effect of ethanol leaf extract of Moringa oleifera in experimentally induced hypercholesterolemic rats was investigated. Thirty six (36) wistar rats of both sexes weighing 130.53±4.86 were used for the study. The animals were completely randomized into six groups (A-F) comprising 6 animals each. Groups A, B and C comprise female rats administered 1 ml of distilled water, high dose of 600 mg/kg and low dose of 300 mg/kg body weight of the extract respectively. Groups D, E and F comprise male rats administered 1 ml of distilled water, high dose of 600 mg/kg and low dose of 300 mg/kg body weight of the extract respectively. Hypercholesterolemia was induced by feeding the animals with high fat diet for 21 days before administration of the extract. After the 21 days of feeding, administration of extract lasted for 14 days. Preliminary phytochemical screening revealed that the ethanol leaf extract of M. oleifera contains alkaloids, tannins, carbohydrates and cardiac glycosides. Only the high dose female group (600 mg/kg body weight) lost or maintained their body weight significantly (p0.05) altered in the male group administered low dose and high dose, showing that the dose of the extract slightly affected their weight. For serum lipids, serum total cholesterol concentration in both male and female reduced significantly (p0.05) affected in other groups. Serum low density lipoprotein cholesterol (LDLC) level also reduced significantly (p0.05) altered for those that received low doses (300 mg/kg body weight) of the extract. There was no significant (p>0.05) reduction in the LDLC of the male rats. Serum triacylglycerol (TAG) concentration in male and female rats reduced significantly (p<0.05), in those that received low and high doses of the extract. Overall, findings from the present study suggest that the ethanol leaf extract of M. oleifera has hypolipidaemic effect. Therefore, the leafy vegetable may be recommended to patients that have problems with high serum lipid profiles and also for people that want to lose or maintain body weight.
    VL  - 3
    IS  - 4
    ER  - 

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Author Information
  • Department of Physiology, Faculty of Basic Medical Sciences, Bingham University, Karu, Nasarawa State, Nigeria

  • Department of Physiology, Faculty of Basic Medical Sciences, Bingham University, Karu, Nasarawa State, Nigeria

  • Department of Biochemistry, Faculty of Science and Technology, Bingham University, Karu, Nasarawa State, Nigeria

  • Department of Physiology, Faculty of Basic Medical Sciences, Bingham University, Karu, Nasarawa State, Nigeria

  • Department of Physiology, Faculty of Basic Medical Sciences, Bingham University, Karu, Nasarawa State, Nigeria

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