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Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass

Received: 31 January 2021     Accepted: 7 February 2021     Published: 23 February 2021
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Abstract

The rumen-mimetic continuous cultivation system (RMS) was used to produce volatile fatty acids (VFAs), such as acetic, propionic, and butyric acids, from lignocellulosic biomass. Typical 12 species of ruminal bacteria were detected in the culture broth cultivated by RMS using species-specific PCR assay. These species are known to be cellulolytic, hemicellulolytic, proteolytic, amylolytic, and lipolytic bacteria and methanogens. Therefore, RMS was characterized to be ruminal fermentation like the digestive system of roughage in the rumen of cattle. Using RMS, the ruminal bacteria were continuously cultivated for 120 days to produce VFAs. The yield of total VFAs from 20–50 μm rice straw was 167% higher than that from 300–1000 μm rice straw. However, the productivity was not increased by pulverization of the substrate. The yield and productivity of acetic acid from 20–50 μm rice straw were 0.147 g/g and 34.8 mg/L/h, respectively, and those of propionic acid were 0.104 g/g and 24.6 mg/L/h, respectively. The selectivity (P/A ratio) of acetic and propionic acid production from microcrystalline cellulose at pH 6.5 and 7.0 were 0.83±0.07 and 0.31±0.03, respectively. The ruminal bacteria could not continue to produce VFAs at pH 6.0. The yield and productivity of acetic acid from microcrystalline cellulose at pH 6.5 were 0.234 g/g and 51.7 mg/L/h, respectively, and those of propionic acid were 0.223 g/g and 49.3 mg/L/h, respectively. This study proposes that in order to increase the productivity of RMS, it is necessary to increase the ruminal bacterial biomass by recycling biomass (flocs) using a settling tank.

Published in International Journal of Microbiology and Biotechnology (Volume 6, Issue 1)
DOI 10.11648/j.ijmb.20210601.11
Page(s) 1-8
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Rumen, Ruminal Bacteria, Volatile Fatty Acid, Acetic Acid, Propionic Acid, Lignocellulosic Biomass

References
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    Hitosi Agematu, Jun Watanabe, Takehiko Takahashi. (2021). Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass. International Journal of Microbiology and Biotechnology, 6(1), 1-8. https://doi.org/10.11648/j.ijmb.20210601.11

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    Hitosi Agematu; Jun Watanabe; Takehiko Takahashi. Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass. Int. J. Microbiol. Biotechnol. 2021, 6(1), 1-8. doi: 10.11648/j.ijmb.20210601.11

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    AMA Style

    Hitosi Agematu, Jun Watanabe, Takehiko Takahashi. Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass. Int J Microbiol Biotechnol. 2021;6(1):1-8. doi: 10.11648/j.ijmb.20210601.11

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  • @article{10.11648/j.ijmb.20210601.11,
      author = {Hitosi Agematu and Jun Watanabe and Takehiko Takahashi},
      title = {Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {6},
      number = {1},
      pages = {1-8},
      doi = {10.11648/j.ijmb.20210601.11},
      url = {https://doi.org/10.11648/j.ijmb.20210601.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20210601.11},
      abstract = {The rumen-mimetic continuous cultivation system (RMS) was used to produce volatile fatty acids (VFAs), such as acetic, propionic, and butyric acids, from lignocellulosic biomass. Typical 12 species of ruminal bacteria were detected in the culture broth cultivated by RMS using species-specific PCR assay. These species are known to be cellulolytic, hemicellulolytic, proteolytic, amylolytic, and lipolytic bacteria and methanogens. Therefore, RMS was characterized to be ruminal fermentation like the digestive system of roughage in the rumen of cattle. Using RMS, the ruminal bacteria were continuously cultivated for 120 days to produce VFAs. The yield of total VFAs from 20–50 μm rice straw was 167% higher than that from 300–1000 μm rice straw. However, the productivity was not increased by pulverization of the substrate. The yield and productivity of acetic acid from 20–50 μm rice straw were 0.147 g/g and 34.8 mg/L/h, respectively, and those of propionic acid were 0.104 g/g and 24.6 mg/L/h, respectively. The selectivity (P/A ratio) of acetic and propionic acid production from microcrystalline cellulose at pH 6.5 and 7.0 were 0.83±0.07 and 0.31±0.03, respectively. The ruminal bacteria could not continue to produce VFAs at pH 6.0. The yield and productivity of acetic acid from microcrystalline cellulose at pH 6.5 were 0.234 g/g and 51.7 mg/L/h, respectively, and those of propionic acid were 0.223 g/g and 49.3 mg/L/h, respectively. This study proposes that in order to increase the productivity of RMS, it is necessary to increase the ruminal bacterial biomass by recycling biomass (flocs) using a settling tank.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Characterization of the Rumen-Mimetic Continuous Cultivation System for Volatile Fatty Acid Production from Lignocellulosic Biomass
    AU  - Hitosi Agematu
    AU  - Jun Watanabe
    AU  - Takehiko Takahashi
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    PY  - 2021
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    DO  - 10.11648/j.ijmb.20210601.11
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 1
    EP  - 8
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20210601.11
    AB  - The rumen-mimetic continuous cultivation system (RMS) was used to produce volatile fatty acids (VFAs), such as acetic, propionic, and butyric acids, from lignocellulosic biomass. Typical 12 species of ruminal bacteria were detected in the culture broth cultivated by RMS using species-specific PCR assay. These species are known to be cellulolytic, hemicellulolytic, proteolytic, amylolytic, and lipolytic bacteria and methanogens. Therefore, RMS was characterized to be ruminal fermentation like the digestive system of roughage in the rumen of cattle. Using RMS, the ruminal bacteria were continuously cultivated for 120 days to produce VFAs. The yield of total VFAs from 20–50 μm rice straw was 167% higher than that from 300–1000 μm rice straw. However, the productivity was not increased by pulverization of the substrate. The yield and productivity of acetic acid from 20–50 μm rice straw were 0.147 g/g and 34.8 mg/L/h, respectively, and those of propionic acid were 0.104 g/g and 24.6 mg/L/h, respectively. The selectivity (P/A ratio) of acetic and propionic acid production from microcrystalline cellulose at pH 6.5 and 7.0 were 0.83±0.07 and 0.31±0.03, respectively. The ruminal bacteria could not continue to produce VFAs at pH 6.0. The yield and productivity of acetic acid from microcrystalline cellulose at pH 6.5 were 0.234 g/g and 51.7 mg/L/h, respectively, and those of propionic acid were 0.223 g/g and 49.3 mg/L/h, respectively. This study proposes that in order to increase the productivity of RMS, it is necessary to increase the ruminal bacterial biomass by recycling biomass (flocs) using a settling tank.
    VL  - 6
    IS  - 1
    ER  - 

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Author Information
  • Department of Applied Chemistry, Akita National College of Technology, Akita, Japan

  • Department of Agribusiness, Akita Prefectural University, Ohgata, Japan

  • Department of Machine Intelligence and Systems Engineering, Akita Prefectural University, Yurihonjo, Japan

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