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Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples

Received: 19 November 2020     Accepted: 4 December 2020     Published: 16 December 2020
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Abstract

Actinomycetes are a filamentous bacterium that belongs to the phyla actinobacteria. They are one of the most attractive sources of antibiotics. In this study, Isolation of Actinomycetes strain was obtained by serial dilution method and grown on starch casine agar (SCA). Soil sample were collected from Addis Ababa Science and Technology University (AASTU) and tests were performed to assess the presence of Actinomycetes using macroscopic observation and microscopic methods. Potential actinomycete isolates were also identified as gram positive by using Gram staining technique. The five out of twelve isolates showed coiled and rod shaped spore resembling the genus Streptomycets and antibacterial activities were evaluated for their antibacterial activity. Three out of five potential actinomycete isolates (isolate number 2, 4, and 5) showed anti bacteria activity, of which one isolate (isolate number 5) showed good inhibitory result when tested against two strains of microorganisms (E.coli and S. aures). Isolate 5 was found to exhibit significant antibacterial activity against the gram positive bacteria, Staphylococcus aures. It is concluded that the biowaste soil sampled from AASTU campus harbors a variety of potential antibiotic producing Actinomycetes, which can be used up for further exploration.

Published in International Journal of Microbiology and Biotechnology (Volume 5, Issue 4)
DOI 10.11648/j.ijmb.20200504.15
Page(s) 203-206
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Actinomycetes, Antibiotics, Streptomycets

References
[1] Goodfellow, M., and Williams, S. T. (1983). Ecology of actinomycetes. Annual review of microbiology. pp. 37 (1): 189-216.
[2] Gouse B. S, Muazzam S. M. Gokul S. S, Ranjith M. S (2017). Isolation and characterization of actinomycetes from soil of ad-dawadmi, saudi arabia and screening their antibacterial activities. International Journal of Pharmacy and Pharmaceutical Sciences. pp. 9 (10): 975-1491.
[3] Kannan R. R, Vincent S. G (2011). Molecular characterization of antagonistic streptomyces isolated from a mangrove swamp. Asian Journal of Biotechnology. pp. 3 (3): 237-243.
[4] Zanane C, Latrache H, Elfazazi K, Zahir H, and Ellouali M (2018). Isolation of actinomycetes from different soils of Beni Amir Morocco.
[5] Alanis AJ (2005). Resistance to antibiotics: are we in the post-antibiotic era? Archives of Medical Research. pp. 36: 697-705.
[6] Baltz RH (2006). Marcel Faber Roundtable, Is our antibiotic pipeline unproductive because of starvation, constitution or lack of inspiration? Journal of Industrial Microbiology and Biotechnology. pp. 33: 507-513.
[7] Ganesan P, David R. H. A, Reegan A. D (2017). Isolation and molecular characterization of actinomycetes with antimicrobial and mosquito larvicidal properties. Beni-Seuf University Journal of Basic and Applied Sciences. pp. 6 (2): 209–217.
[8] Rotich, M. C. (2018) Bio-Prospecting for Broad Spectrum Antibiotic Producing Actinomycetes Isolated from Virgin Soils in Kericho County (Doctoral dissertation, JKUAT-COHES).
[9] Kuster E, Williams ST (1964). Selective media for the isolation of Streptomycetes. Nature, pp. 202: 928-929.
[10] Kawato M, Shinobu R (1979). A simple technique for the microscopical observation. Memoirs of the OsakaUniversity Liberal Arts and Education. pp. 8: 114.
[11] Abo-Shadi M, Sidkey NM, Al-Mutrafy AM (2010). Antimicrobial agent producing microbes from some soils’ rhizosphere. The Journal of American Science. pp. 6 (10): 915-925.
[12] Parmar R. S, Singh C, Saini P. and Kumar A. (2016). Isolation and screening of antimicrobial and extracellular pigment producing actinomycetes from Chambal territory of Madya Pradesh region, India. Asian Journal of Pharmaceutical and Clinical Research. pp. 9 (1): 157–160.
[13] Nanjwade B. K, Chandrashekhara S, Shamarez A. M, Goudanavar S. P, Manvi V. F (2010). Isolation and morphological characterization of antibiotic producing actinomycetes. Tropical Journal of Pharmaceutical Research. pp. 9 (3): 231-236.
[14] Wahab A, Shumaila S, Subhan S. A, Ali S. T. and Mujahid T. Y (2015). Isolation and identification of actinomycetes isolated from Karachi soil and screening of antimicrobial compounds. International Journal of Current Research. pp. 7 (2): 12760–12765.
[15] Okudoh, V. I. (2001). Isolation and identification of antibiotic producing microorganisms from natural habitats in the KwaZulu-Natal midlands (Doctoral dissertation).
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  • APA Style

    Rahel Tilahun. (2020). Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples. International Journal of Microbiology and Biotechnology, 5(4), 203-206. https://doi.org/10.11648/j.ijmb.20200504.15

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    ACS Style

    Rahel Tilahun. Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples. Int. J. Microbiol. Biotechnol. 2020, 5(4), 203-206. doi: 10.11648/j.ijmb.20200504.15

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    AMA Style

    Rahel Tilahun. Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples. Int J Microbiol Biotechnol. 2020;5(4):203-206. doi: 10.11648/j.ijmb.20200504.15

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  • @article{10.11648/j.ijmb.20200504.15,
      author = {Rahel Tilahun},
      title = {Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {5},
      number = {4},
      pages = {203-206},
      doi = {10.11648/j.ijmb.20200504.15},
      url = {https://doi.org/10.11648/j.ijmb.20200504.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200504.15},
      abstract = {Actinomycetes are a filamentous bacterium that belongs to the phyla actinobacteria. They are one of the most attractive sources of antibiotics. In this study, Isolation of Actinomycetes strain was obtained by serial dilution method and grown on starch casine agar (SCA). Soil sample were collected from Addis Ababa Science and Technology University (AASTU) and tests were performed to assess the presence of Actinomycetes using macroscopic observation and microscopic methods. Potential actinomycete isolates were also identified as gram positive by using Gram staining technique. The five out of twelve isolates showed coiled and rod shaped spore resembling the genus Streptomycets and antibacterial activities were evaluated for their antibacterial activity. Three out of five potential actinomycete isolates (isolate number 2, 4, and 5) showed anti bacteria activity, of which one isolate (isolate number 5) showed good inhibitory result when tested against two strains of microorganisms (E.coli and S. aures). Isolate 5 was found to exhibit significant antibacterial activity against the gram positive bacteria, Staphylococcus aures. It is concluded that the biowaste soil sampled from AASTU campus harbors a variety of potential antibiotic producing Actinomycetes, which can be used up for further exploration.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Isolation of Antibiotics Producing Actinobacteria from Biowaste Soil Samples
    AU  - Rahel Tilahun
    Y1  - 2020/12/16
    PY  - 2020
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    DO  - 10.11648/j.ijmb.20200504.15
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 203
    EP  - 206
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20200504.15
    AB  - Actinomycetes are a filamentous bacterium that belongs to the phyla actinobacteria. They are one of the most attractive sources of antibiotics. In this study, Isolation of Actinomycetes strain was obtained by serial dilution method and grown on starch casine agar (SCA). Soil sample were collected from Addis Ababa Science and Technology University (AASTU) and tests were performed to assess the presence of Actinomycetes using macroscopic observation and microscopic methods. Potential actinomycete isolates were also identified as gram positive by using Gram staining technique. The five out of twelve isolates showed coiled and rod shaped spore resembling the genus Streptomycets and antibacterial activities were evaluated for their antibacterial activity. Three out of five potential actinomycete isolates (isolate number 2, 4, and 5) showed anti bacteria activity, of which one isolate (isolate number 5) showed good inhibitory result when tested against two strains of microorganisms (E.coli and S. aures). Isolate 5 was found to exhibit significant antibacterial activity against the gram positive bacteria, Staphylococcus aures. It is concluded that the biowaste soil sampled from AASTU campus harbors a variety of potential antibiotic producing Actinomycetes, which can be used up for further exploration.
    VL  - 5
    IS  - 4
    ER  - 

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Author Information
  • Department of Biotechnology, School of Biological and Chemical Science, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia

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