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Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests

Received: 27 April 2017     Accepted: 22 May 2017     Published: 16 June 2017
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Abstract

Lactobacillus gasseri G4 strain isolated from healthy human volunteer was selected as one of the best adhesive strain among a large group of lactobacilli. In-vitro tests for establishment of immunomodulatory potential of selected lactic acid bacteria were carried out. Three different analytical models were used for evaluation, according to the specifics of each different cytokine and to compare, where possible. The induction of cytokines like: interleukin-1 (IL-1), IL-10 and tumor necrosis factor alpha (TNF-α) were determined by widely used laboratory approach with mouse splenocytes as well as on combined (well-in-well) monocytic and epithelium human cell lines (THP-1 and HT-29). Other cytokines like IL-8 and IL-4 were evaluated by only one analytical model, while transforming growth factor beta (TGF-β) was evaluated by using all of the three models. The selected strain shows significant increase of IL-10, interferon gamma (IFN-γ) and TGF-β production in-vitro. Therefore, this strain was directed to clinical trials with 38 healthy volunteers, randomized and placebo-controlled. The results demonstrate statistically significant increase of TGF-β production (25%). Immunoglobulin A (IgA) and IFN-γ were also increased. Stable levels of IL-10 were observed after the in-vivo experiments with L. gasseri G4 strain. The selected strain does not increase in-vivo the pro-inflammatory cytokine IL-8. In this study the immunomodulatory properties of L. gasseri G4 were confirmed in-vivo therefore it is suitable for development of probiotic formulas.

Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 4)
DOI 10.11648/j.ijmb.20170204.11
Page(s) 156-160
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Probiotics, Lactobacillus gasseri, Immunomodulation, Cytokines, Immunoglobulin

References
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Cite This Article
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    Irina Marinova Gotova, Zhechko Panayotov Dimitrov, Hristo Miladinov Naidenski. (2017). Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests. International Journal of Microbiology and Biotechnology, 2(4), 156-160. https://doi.org/10.11648/j.ijmb.20170204.11

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    ACS Style

    Irina Marinova Gotova; Zhechko Panayotov Dimitrov; Hristo Miladinov Naidenski. Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests. Int. J. Microbiol. Biotechnol. 2017, 2(4), 156-160. doi: 10.11648/j.ijmb.20170204.11

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    AMA Style

    Irina Marinova Gotova, Zhechko Panayotov Dimitrov, Hristo Miladinov Naidenski. Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests. Int J Microbiol Biotechnol. 2017;2(4):156-160. doi: 10.11648/j.ijmb.20170204.11

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  • @article{10.11648/j.ijmb.20170204.11,
      author = {Irina Marinova Gotova and Zhechko Panayotov Dimitrov and Hristo Miladinov Naidenski},
      title = {Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {2},
      number = {4},
      pages = {156-160},
      doi = {10.11648/j.ijmb.20170204.11},
      url = {https://doi.org/10.11648/j.ijmb.20170204.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170204.11},
      abstract = {Lactobacillus gasseri G4 strain isolated from healthy human volunteer was selected as one of the best adhesive strain among a large group of lactobacilli. In-vitro tests for establishment of immunomodulatory potential of selected lactic acid bacteria were carried out. Three different analytical models were used for evaluation, according to the specifics of each different cytokine and to compare, where possible. The induction of cytokines like: interleukin-1 (IL-1), IL-10 and tumor necrosis factor alpha (TNF-α) were determined by widely used laboratory approach with mouse splenocytes as well as on combined (well-in-well) monocytic and epithelium human cell lines (THP-1 and HT-29). Other cytokines like IL-8 and IL-4 were evaluated by only one analytical model, while transforming growth factor beta (TGF-β) was evaluated by using all of the three models. The selected strain shows significant increase of IL-10, interferon gamma (IFN-γ) and TGF-β production in-vitro. Therefore, this strain was directed to clinical trials with 38 healthy volunteers, randomized and placebo-controlled. The results demonstrate statistically significant increase of TGF-β production (25%). Immunoglobulin A (IgA) and IFN-γ were also increased. Stable levels of IL-10 were observed after the in-vivo experiments with L. gasseri G4 strain. The selected strain does not increase in-vivo the pro-inflammatory cytokine IL-8. In this study the immunomodulatory properties of L. gasseri G4 were confirmed in-vivo therefore it is suitable for development of probiotic formulas.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests
    AU  - Irina Marinova Gotova
    AU  - Zhechko Panayotov Dimitrov
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    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
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    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20170204.11
    AB  - Lactobacillus gasseri G4 strain isolated from healthy human volunteer was selected as one of the best adhesive strain among a large group of lactobacilli. In-vitro tests for establishment of immunomodulatory potential of selected lactic acid bacteria were carried out. Three different analytical models were used for evaluation, according to the specifics of each different cytokine and to compare, where possible. The induction of cytokines like: interleukin-1 (IL-1), IL-10 and tumor necrosis factor alpha (TNF-α) were determined by widely used laboratory approach with mouse splenocytes as well as on combined (well-in-well) monocytic and epithelium human cell lines (THP-1 and HT-29). Other cytokines like IL-8 and IL-4 were evaluated by only one analytical model, while transforming growth factor beta (TGF-β) was evaluated by using all of the three models. The selected strain shows significant increase of IL-10, interferon gamma (IFN-γ) and TGF-β production in-vitro. Therefore, this strain was directed to clinical trials with 38 healthy volunteers, randomized and placebo-controlled. The results demonstrate statistically significant increase of TGF-β production (25%). Immunoglobulin A (IgA) and IFN-γ were also increased. Stable levels of IL-10 were observed after the in-vivo experiments with L. gasseri G4 strain. The selected strain does not increase in-vivo the pro-inflammatory cytokine IL-8. In this study the immunomodulatory properties of L. gasseri G4 were confirmed in-vivo therefore it is suitable for development of probiotic formulas.
    VL  - 2
    IS  - 4
    ER  - 

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Author Information
  • R&D Center LB Bulgaricum Plc, Sofia, Bulgaria

  • R&D Center LB Bulgaricum Plc, Sofia, Bulgaria

  • The Stefan Angelov Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

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