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Zinc Solubilizing Bacteria from Rhizospheric Soil of Mangroves

Received: 6 March 2017     Accepted: 24 March 2017     Published: 8 May 2017
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Abstract

Zinc (Zn) is among the essential micronutrients required for optimum plant growth. Inorganic zinc in soil is generally in unavailable form for plant assimilation. However, Zinc Solubilizing Bacteria (ZSB) makes the inorganic zinc in to biologically available form. Such studies in mangroves habitats are almost non-existing. Hence, the present study explored the presence of ZSB from mangrove soil. The ZSB were in a range of 9.53% to 13.9% in non-mangrove soil and Rhizophora mangrove root soil respectively. Out of 24 morphologically distinct strains of ZSB, three strains (ZSB-4, ZSB-13, ZSB-14) displayed high Zn solubilization efficiency on solid medium amended with ZnO (382%), ZnCO3 (365%) and ZnSO4 (336%). These strains exhibited significant release of Zn at the concentrations of 2.3 2.12 and 2.09 ppm by ZSB-14, ZSB-4 and ZSB-13 respectively on 10th day of incubation in broth medium amended with ZnO. The strains released acids as evident by decline in pH of the broth medium. They also secreted IAA with the maximum of 14.5 ppm by ZSB-4 with ZnO as source of Zn. The potential strains for Zn solubilization were identified using 16S rRNA as Pseudomonas aeroginosa for further application as bioinoculants to mangrove soil.

Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 3)
DOI 10.11648/j.ijmb.20170203.17
Page(s) 148-155
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Mangroves, Rhizospheric Soil, Zinc Solubilizing Bacteria, Indole 3 Acetic Acid

References
[1] Parisi, B. and Vallee, B. L. 1969. Metal enzyme complexes activated by zinc. J. Biol. Chem. 179: 803-807.
[2] Normanly, J., Cohen, J. D. and Fink, G. R. 1993. Arabidopsis thaliana auxotrophs reveal a tryptophan-independent biosynthetic pathway for indole-3-acetic acid. Proc. Natl. Acad. Sci. USA, 90(21), 10355–10359.
[3] Brown, P. H., Cakmak, I. and Zhang, Q. 1993. Form and function of Zinc in soils and plants. Chapter 7 in Robson A. D. (ed) Zinc in soils and plants, Kluwerb Acadamic Publishers, Dordrecht pp. 90 – 106.
[4] Cakmak, I. 2009. Enrichment of fertilizers with zinc: an excellent investment for humanity and crop production in India. J. Trace Elem. Med. Biol., 23, 281–289.
[5] Srivastava, P. C. and Gupta, U. C. 1996. Trace Elements in Crop Production, Oxford and IBH Publishers, New Delhi, 356pp.
[6] Krishnaswamy, R. 1993. Effect of phosphatic fertilization on zinc adsorption in some vertisol and inceptisol. J. Indian. Soc. oil Sci., 41, 251–255.
[7] Cunninghan, J. E. and Kuiack, C. 1992. Production of citric acid and oxalic acid and solubilization of calcium phosphate by Penicillium billai. Appl. Environ. Microbio l.58, 1451–1458.
[8] Irum Naz., Habib Ahmad., Shahida Nasreen Khokhar., Khalid Khan. and Azhar Hussain Shah. 2016. Impact of Zinc Solubilizing Bacteria on Zinc Contents of Wheat. American-Eurasian J. Agric. & Environ. Sci., 16 (3): 449-454.
[9] Sunithakumari, K., Padma Devi, S. N. and Vasandha, S. 2016. Zinc solubilizing bacterial isolates from the agricultural fields of Coimbatore, Tamil Nadu, India, Current Science, 110, 196 2, 25.
[10] Kathiresan, K. and Bingham, B. L. 2001. Biology of mangroves and mangrove ecosystems. Advances in Marine Biology. 40: 81-251.
[11] Mackey, A. P. and Mackay, S. 1996. Environ. Pollut. 93: 205.
[12] Lacerda, L. D., Carvalho, C. E. V., Tanizaki, K. F., Ovalle, A. R. C. and Rezende, C. E. 1993. The biogeochemistry and trace metals distribution of mangrove rhizospheres. Biotropica 25: 252-257.
[13] Piper, C. S., 1966. Soil plant analysis. Hans publications, Bombay.
[14] EI Wakeel S. K and Riley, J. P. 1956. The determination of organic carbon in marine muds, J. Cons., 22: 180 -183.
[15] Subbaih, B. V. and Asija, G. L. 1956. A rapid procedure for the determination of available nitrogen in soils. Curr. Sci. 31: 196.
[16] Olsen, S. R., Cole, C. V., Watanabe, F. S. and Dean. 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. Circ. U. S. Dep. Agric., 939.
[17] Guzman, H. and Jimenez, C. E. 1992. Contamination of coral reefs by heavy metals along the Carribbean coast of Central America. (Costal Rica and Panama). Mar. Poll. Bull. 24(11): 554-561.
[18] Lindsay, W. L. and Norvell, W. A. 1978. Development of DTPA Soil Test for Zinc, Iron, Manganese and Copper. Soil Sci. Soc.Am. J., 42: 421 – 428.
[19] Buchanan R. E. and Gibbons, N. E., eds. 1974. Bergey's Manual of Determinative Bacteriology. 8th ed. Williams & Wilkins Co., Baltimore, Md. 21202. Xxvi + 1246 pp. $45.00.
[20] Nyugen, C., Yan, W., Tacon, F. L. and Lapyire, F. 1992. Genetic variability of phosphate solubilizing activity by monocaryotic and dicaryotic mycelia of the ectomycorrhizal fungus Laccaria bicolor (Maire). Plant Soil, 143, 193–199.
[21] Drummond A. J. Ashton B. Buxton S. Swidan. Cheun M. Duran C. Heled J. Kearse M. Markowitz S. Moir R. Stones-Havas S. Sturrock S. Swidan F. Thierer T. Wilson A. (2012) Geneious v 5.6, Available from http://www.geneious.com
[22] Francis, A. J., Dodge, C., Chendrayan, K. and Quinby, H. 1988. An-aerobic microbial dissolution of lead oxide and production of organicacids. US Patent No. 4758345.
[23] Brick, J. M., Bostock, R. M. and Silverstone, S. E. 1991. Rapid in situ assay for indole acetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl. Environ. Microbiol. 57(2), 535–538.
[24] Duncan, B. D. 1957. Multiple range test for correlated and heteroscedatic means. Biometrics, 13: 359-364.
[25] Rickard, W. H. 1965. The influence of greasewool on soil mixture penetration and soil chemistry. Northwest Sci. 39: 36–42.
[26] Munshower, F. F. 1993. Practical hand book of disturbed land revegetation. Lewis Publishers, London. Tokyo.
[27] Lowe, L. E. (1975) Fractionation of acid-soluble components of soil organic matter using polyvinyl pyrrolidone. Can. J. Soil. Sci. 60: 219 -229.
[28] Ajmal, M and Khan, A. V. (1987). Heavy metals in water sediments, fish and plants of river Hindson, U. P., India. Hydrobiologia. 148: 151-157.
[29] Marschner, H. 1995. Mineral nutrition of higher plants. Second Edition, Academic press, London.
[30] Robson, A. D. and Pitman, M. G. 1983. Interactions between nutrients in higher plants. In: Lauchli, A., Bieleski, R. L. eds. Encyclopedia of plant physiology. Vol 15A. New series, Berlin and New York: Springer-Verlag, pp. 287-312.
[31] Fasim, F., Ahmed, N., Parsons, R. and Gadd, G. M. 2002. Solubilzation of zinc salts by bacterium isolated from the air environment of a tannery. FEMS Microbiol. Lett., 213, 1–6.
[32] Saravanan, V. S., Subramanian, S. and Anthoni Raj, S. 2003. Assessing in vitro solubilization potential of different zinc solubilizing bacterial isolates. Braz. J. Microbiol., 34: 121–125.
[33] Sarathambal, C., Thangaraju, M., Paulraj, C. and Gomathy, M. 2010. Assessing the Zinc solubilization ability of Gluconacetobacter diazotrophicus in maize rhizosphere using labelled 65Zn compounds. Indian J Microbiol. 50 (1): 103–109.
[34] Henri, F., Laurette, N., Annette, D., John, Q., Wolfgang, M. and Xavier, F., 2008, Solubilization of inorganic phosphates and plant growth promotionby strains of Pseudomonas fluorescens isolated from acidic soils of Cameroon. Afr. J. Microbiol. Res., 2, 171–178.
[35] Lin, T. F., Huang, H. I., Shen, F. T. and Young, C. C. 2006,, The protons of gluconic acid are the major factors responsible for the dissolu-tion of tricalcium phosphate by Burkholderia cepacia CC-A174. Bioresour. Technol., 97, 957–960.
[36] Song, O. R., Lee, S. J., Kim, S. H., Chung, S. Y., Cha, I. H. and Choi, Y. L., 2008, Isolation and cultural characteristics of a phosphate-solubilizing bacterium, Aeromonas hydrophila DA 57. J. Korean Soc. Agric. Chem. Biotechnol., 44, 257–261.
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  • APA Style

    Beulah Jerlin, S. Sharmila, K. Kathiresan, K. Kayalvizhi. (2017). Zinc Solubilizing Bacteria from Rhizospheric Soil of Mangroves. International Journal of Microbiology and Biotechnology, 2(3), 148-155. https://doi.org/10.11648/j.ijmb.20170203.17

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    ACS Style

    Beulah Jerlin; S. Sharmila; K. Kathiresan; K. Kayalvizhi. Zinc Solubilizing Bacteria from Rhizospheric Soil of Mangroves. Int. J. Microbiol. Biotechnol. 2017, 2(3), 148-155. doi: 10.11648/j.ijmb.20170203.17

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    AMA Style

    Beulah Jerlin, S. Sharmila, K. Kathiresan, K. Kayalvizhi. Zinc Solubilizing Bacteria from Rhizospheric Soil of Mangroves. Int J Microbiol Biotechnol. 2017;2(3):148-155. doi: 10.11648/j.ijmb.20170203.17

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  • @article{10.11648/j.ijmb.20170203.17,
      author = {Beulah Jerlin and S. Sharmila and K. Kathiresan and K. Kayalvizhi},
      title = {Zinc Solubilizing Bacteria from Rhizospheric Soil of Mangroves},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {2},
      number = {3},
      pages = {148-155},
      doi = {10.11648/j.ijmb.20170203.17},
      url = {https://doi.org/10.11648/j.ijmb.20170203.17},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170203.17},
      abstract = {Zinc (Zn) is among the essential micronutrients required for optimum plant growth. Inorganic zinc in soil is generally in unavailable form for plant assimilation. However, Zinc Solubilizing Bacteria (ZSB) makes the inorganic zinc in to biologically available form. Such studies in mangroves habitats are almost non-existing. Hence, the present study explored the presence of ZSB from mangrove soil. The ZSB were in a range of 9.53% to 13.9% in non-mangrove soil and Rhizophora mangrove root soil respectively. Out of 24 morphologically distinct strains of ZSB, three strains (ZSB-4, ZSB-13, ZSB-14) displayed high Zn solubilization efficiency on solid medium amended with ZnO (382%), ZnCO3 (365%) and ZnSO4 (336%). These strains exhibited significant release of Zn at the concentrations of 2.3 2.12 and 2.09 ppm by ZSB-14, ZSB-4 and ZSB-13 respectively on 10th day of incubation in broth medium amended with ZnO. The strains released acids as evident by decline in pH of the broth medium. They also secreted IAA with the maximum of 14.5 ppm by ZSB-4 with ZnO as source of Zn. The potential strains for Zn solubilization were identified using 16S rRNA as Pseudomonas aeroginosa for further application as bioinoculants to mangrove soil.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Zinc Solubilizing Bacteria from Rhizospheric Soil of Mangroves
    AU  - Beulah Jerlin
    AU  - S. Sharmila
    AU  - K. Kathiresan
    AU  - K. Kayalvizhi
    Y1  - 2017/05/08
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijmb.20170203.17
    DO  - 10.11648/j.ijmb.20170203.17
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 148
    EP  - 155
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20170203.17
    AB  - Zinc (Zn) is among the essential micronutrients required for optimum plant growth. Inorganic zinc in soil is generally in unavailable form for plant assimilation. However, Zinc Solubilizing Bacteria (ZSB) makes the inorganic zinc in to biologically available form. Such studies in mangroves habitats are almost non-existing. Hence, the present study explored the presence of ZSB from mangrove soil. The ZSB were in a range of 9.53% to 13.9% in non-mangrove soil and Rhizophora mangrove root soil respectively. Out of 24 morphologically distinct strains of ZSB, three strains (ZSB-4, ZSB-13, ZSB-14) displayed high Zn solubilization efficiency on solid medium amended with ZnO (382%), ZnCO3 (365%) and ZnSO4 (336%). These strains exhibited significant release of Zn at the concentrations of 2.3 2.12 and 2.09 ppm by ZSB-14, ZSB-4 and ZSB-13 respectively on 10th day of incubation in broth medium amended with ZnO. The strains released acids as evident by decline in pH of the broth medium. They also secreted IAA with the maximum of 14.5 ppm by ZSB-4 with ZnO as source of Zn. The potential strains for Zn solubilization were identified using 16S rRNA as Pseudomonas aeroginosa for further application as bioinoculants to mangrove soil.
    VL  - 2
    IS  - 3
    ER  - 

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Author Information
  • Department of Botany, St. Mary’s College (Autonomous), Thoothukudi, India

  • Department of Botany, Vellalar College for Women (Autonomous), Erode, India

  • Faculty of Marine Sciences, Annamalai University, Chidambaram, India

  • Faculty of Marine Sciences, Annamalai University, Chidambaram, India

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