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Characterization of Potential Pathogenic Bacterial Isolates from Urban and Rural Market Dumpsites

Received: 4 March 2017     Accepted: 18 March 2017     Published: 18 April 2017
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Abstract

Antimicrobial susceptibility and toxigenicity tests were carried out on bacterial species isolated from the soil samples collected from an urban market (Oba market) and a rural market (Ekiadolor market) waste dumpsites. The bacterial species included Escherichia coli, Shigella sp, Staphylococcus aureus, Salmonella sp, Bacillus sp, Enterococcus sp, Clostridium sp, Proteus sp, Klebsiella sp and Pseudomonas sp. E. coli had the highest percentage occurrence of 56.0% in the urban market waste dumpsite and Enterococcus sp had the highest percentage occurrence of 28.13% in the rural market waste dumpsite. Toxigenicity test was carried out on S. aureus, Salmonella sp and E. coli isolates to ascertain enterotoxin production using the rabbit ileal loop assay. S. aureus (60%), Salmonella sp (50%) and E. coli (62.5%) isolates were positive to enterotoxin production. Ciprofloxacin (CIP), Peflacin (PEF), Tetracycline (TET) and Gentamycin (GEN) were the most effective antibiotics against the bacterial isolates. Antimicrobial susceptibility of the bacterial isolates from the urban waste dumpsite was CIP (40.74%), PEF (35.19%) and GEN (33.33%) and antimicrobial susceptibility of the bacterial isolates from the rural waste dumpsite was CIP (51.43%), TET (40.00%) and GEN (42.86%). The antibiotics were more effective against the bacterial isolates from the rural market dump site. Pollution of the environment by wastes from markets is of public health concern because the presence of pathogenic bacteria in the wastes has the potential of contaminating air, soil and ground water.

Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 3)
DOI 10.11648/j.ijmb.20170203.14
Page(s) 129-134
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Bacterial Isolates, Dumpsites, Market, Pathogenic, Rural, Urban

References
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    Iyobosa Benedicta Idahosa, Henrietta Omokhele Obueh, Millicent Omoyemhe Aigbekaen, Eunice Asiboroko Odesiri - Eruteyan. (2017). Characterization of Potential Pathogenic Bacterial Isolates from Urban and Rural Market Dumpsites. International Journal of Microbiology and Biotechnology, 2(3), 129-134. https://doi.org/10.11648/j.ijmb.20170203.14

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    ACS Style

    Iyobosa Benedicta Idahosa; Henrietta Omokhele Obueh; Millicent Omoyemhe Aigbekaen; Eunice Asiboroko Odesiri - Eruteyan. Characterization of Potential Pathogenic Bacterial Isolates from Urban and Rural Market Dumpsites. Int. J. Microbiol. Biotechnol. 2017, 2(3), 129-134. doi: 10.11648/j.ijmb.20170203.14

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    AMA Style

    Iyobosa Benedicta Idahosa, Henrietta Omokhele Obueh, Millicent Omoyemhe Aigbekaen, Eunice Asiboroko Odesiri - Eruteyan. Characterization of Potential Pathogenic Bacterial Isolates from Urban and Rural Market Dumpsites. Int J Microbiol Biotechnol. 2017;2(3):129-134. doi: 10.11648/j.ijmb.20170203.14

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  • @article{10.11648/j.ijmb.20170203.14,
      author = {Iyobosa Benedicta Idahosa and Henrietta Omokhele Obueh and Millicent Omoyemhe Aigbekaen and Eunice Asiboroko Odesiri - Eruteyan},
      title = {Characterization of Potential Pathogenic Bacterial Isolates from Urban and Rural Market Dumpsites},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {2},
      number = {3},
      pages = {129-134},
      doi = {10.11648/j.ijmb.20170203.14},
      url = {https://doi.org/10.11648/j.ijmb.20170203.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170203.14},
      abstract = {Antimicrobial susceptibility and toxigenicity tests were carried out on bacterial species isolated from the soil samples collected from an urban market (Oba market) and a rural market (Ekiadolor market) waste dumpsites. The bacterial species included Escherichia coli, Shigella sp, Staphylococcus aureus, Salmonella sp, Bacillus sp, Enterococcus sp, Clostridium sp, Proteus sp, Klebsiella sp and Pseudomonas sp. E. coli had the highest percentage occurrence of 56.0% in the urban market waste dumpsite and Enterococcus sp had the highest percentage occurrence of 28.13% in the rural market waste dumpsite. Toxigenicity test was carried out on S. aureus, Salmonella sp and E. coli isolates to ascertain enterotoxin production using the rabbit ileal loop assay. S. aureus (60%), Salmonella sp (50%) and E. coli (62.5%) isolates were positive to enterotoxin production. Ciprofloxacin (CIP), Peflacin (PEF), Tetracycline (TET) and Gentamycin (GEN) were the most effective antibiotics against the bacterial isolates. Antimicrobial susceptibility of the bacterial isolates from the urban waste dumpsite was CIP (40.74%), PEF (35.19%) and GEN (33.33%) and antimicrobial susceptibility of the bacterial isolates from the rural waste dumpsite was CIP (51.43%), TET (40.00%) and GEN (42.86%). The antibiotics were more effective against the bacterial isolates from the rural market dump site. Pollution of the environment by wastes from markets is of public health concern because the presence of pathogenic bacteria in the wastes has the potential of contaminating air, soil and ground water.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Characterization of Potential Pathogenic Bacterial Isolates from Urban and Rural Market Dumpsites
    AU  - Iyobosa Benedicta Idahosa
    AU  - Henrietta Omokhele Obueh
    AU  - Millicent Omoyemhe Aigbekaen
    AU  - Eunice Asiboroko Odesiri - Eruteyan
    Y1  - 2017/04/18
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ijmb.20170203.14
    DO  - 10.11648/j.ijmb.20170203.14
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 129
    EP  - 134
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20170203.14
    AB  - Antimicrobial susceptibility and toxigenicity tests were carried out on bacterial species isolated from the soil samples collected from an urban market (Oba market) and a rural market (Ekiadolor market) waste dumpsites. The bacterial species included Escherichia coli, Shigella sp, Staphylococcus aureus, Salmonella sp, Bacillus sp, Enterococcus sp, Clostridium sp, Proteus sp, Klebsiella sp and Pseudomonas sp. E. coli had the highest percentage occurrence of 56.0% in the urban market waste dumpsite and Enterococcus sp had the highest percentage occurrence of 28.13% in the rural market waste dumpsite. Toxigenicity test was carried out on S. aureus, Salmonella sp and E. coli isolates to ascertain enterotoxin production using the rabbit ileal loop assay. S. aureus (60%), Salmonella sp (50%) and E. coli (62.5%) isolates were positive to enterotoxin production. Ciprofloxacin (CIP), Peflacin (PEF), Tetracycline (TET) and Gentamycin (GEN) were the most effective antibiotics against the bacterial isolates. Antimicrobial susceptibility of the bacterial isolates from the urban waste dumpsite was CIP (40.74%), PEF (35.19%) and GEN (33.33%) and antimicrobial susceptibility of the bacterial isolates from the rural waste dumpsite was CIP (51.43%), TET (40.00%) and GEN (42.86%). The antibiotics were more effective against the bacterial isolates from the rural market dump site. Pollution of the environment by wastes from markets is of public health concern because the presence of pathogenic bacteria in the wastes has the potential of contaminating air, soil and ground water.
    VL  - 2
    IS  - 3
    ER  - 

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Author Information
  • Department of Biology, College of Education, Ekiadolor, Benin City, Nigeria

  • Department of Biology, College of Education, Ekiadolor, Benin City, Nigeria

  • Department of Biology, College of Education, Ekiadolor, Benin City, Nigeria

  • Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Warri, Nigeria

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