The production of indole acetic acid (IAA) by plant growth-promoting microorganisms is one of the most important factors for plant growth. Present research work deals with the characterization and optimization of different physiological conditions for IAA production by the fungus Talaromyces trachyspermus. Different environmental factors and medium components were optimized for the production of IAA by fungal culture. Effect of pH, temperature, aeration and concentration of precursor i.e. L-Tryptophan was evaluated on the biomass of fungus along with the IAA production. This work also focused on the effect of different carbon and nitrogen sources in the media for the growth of Talaromyces trachyspermus and IAA production The maximum production of IAA was observed in 15 days of incubation under the condition of 6.5 pH, 28°C temperature, and 140 RPM, in presence of 0.5% of tryptophan, 4% glucose and 0.1% sodium nitrate. After optimization, the yield of IAA by fungal culture was increased up to 0.78 fold as compared to initial production. IAA production by fungal culture was confirmed by thin-layer chromatography and HPLC analysis. Optimization of IAA production by Talaromyces trachyspermus can be useful for the large-scale production of agriculturally important bioactive metabolites. Moreover, the plant growth-promoting efficiency of this fungal culture makes it novel bio-inoculants for sustainable agriculture.
| Published in | Frontiers in Environmental Microbiology (Volume 8, Issue 2) |
| DOI | 10.11648/j.fem.20220802.12 |
| Page(s) | 25-34 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Indole Acetic Acid, Plant Growth-Promoting Fungi, Talaromyces trachyspermus, Optimization, Sustainable Agriculture
| [1] | Mendes R, Garbeva P, Raaijmakers JM. The rhizosphere microbiome: Significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms. FEMS Microbiol Rev. 2013; 37: 634–63. |
| [2] | Barriuso J, Ramos Solano B, Lucas JA, Lobo AP, García-Villaraco A, Gutiérrez Mañero FJ. Ecology, Genetic Diversity and Screening Strategies of Plant Growth Promoting Rhizobacteria (PGPR). Plant-Bacteria Interact Strateg Tech to Promot Plant Growth. 2008; 1–17. |
| [3] | Gahan J, Schmalenberger A. The role of bacteria and mycorrhiza in plant sulfur supply. Front Plant Sci [Internet]. Frontiers Media S. A.; 2014; 5: 723. Available from: https://pubmed.ncbi.nlm.nih.gov/25566295 |
| [4] | Hunter MC, Smith RG, Schipanski ME, Atwood LW, Mortensen DA. Agriculture in 2050: Recalibrating targets for sustainable intensification. Bioscience. 2017; 67: 386–91. |
| [5] | Lakshmanan V, Selvaraj G, Bais HP. Functional Soil Microbiome: Belowground Solutions to an Aboveground Problem. Plant Physiol [Internet]. 2014; 166: 689–700. Available from: https://doi.org/10.1104/pp.114.245811 |
| [6] | Hossain MM, Sultana F, Islam S. Plant growth-promoting fungi (PGPF): Phytostimulation and induced systemic resistance. Plant-Microbe Interact. Agro-Ecological Perspect. 2017. |
| [7] | Hossain MM, Sultana F, Kubota M, Koyama H, Hyakumachi M. The Plant Growth-Promoting Fungus Penicillium simplicissimum GP17-2 Induces Resistance in Arabidopsis thaliana by Activation of Multiple Defense Signals. Plant Cell Physiol [Internet]. 2007; 48: 1724–36. Available from: https://doi.org/10.1093/pcp/pcm144 |
| [8] | Hossain MM, Sultana F, Miyazawa M, Hyakumachi M. The Plant Growth-promoting Fungus Penicillium spp. GP15-1 Enhances Growth and Confers Protection against Damping-off and Anthracnose in the Cucumber. J Oleo Sci. 2014; 63: 391–400. |
| [9] | Shoresh M, Harman GE, Mastouri F. Induced systemic resistance and plant responses to fungal biocontrol agents. Annu Rev Phytopathol. 2010; 48: 21–43. |
| [10] | Hyakumachi M. Plant-Growth-Promoting Fungi from Turfgrass Rhizosphere with Potential for Disease Suppression. Soil Microorg. 1994; 44: 53–68. |
| [11] | Harman GE, Petzoldt R, Comis A, Chen J. Interactions Between Trichoderma harzianum Strain T22 and Maize Inbred Line Mo17 and Effects of These Interactions on Diseases Caused by Pythium ultimum and Colletotrichum graminicola. Phytopathology® [Internet]. Scientific Societies; 2004; 94: 147–53. Available from: https://doi.org/10.1094/PHYTO.2004.94.2.147 |
| [12] | Naznin HA, Kimura M, Miyazawa M, Hyakumachi M. Analysis of Volatile Organic Compounds Emitted by Plant Growth-Promoting Fungus Phoma sp. GS8-3 for Growth Promotion Effects on Tobacco. Microbes Environ. 2013; 28: 42–9. |
| [13] | Wakelin SA, Gupta VVSR, Harvey PR, Ryder MH. The effect of Penicillium fungi on plant growth and phosphorus mobilization in neutral to alkaline soils from southern Australia. Can J Microbiol [Internet]. NRC Research Press; 2007; 53: 106–15. Available from: https://doi.org/10.1139/w06-109 |
| [14] | Datta C, Basu PS. Indole acetic acid production by a. Rhizobium species from root nodules of a leguminious shrub, Cajanus cajan. Microbiol Res. 2000; 155: 123: 123–7. Available from: Https://www.sciencedirect.com/science/article/pii/S0944501300800476 |
| [15] | Spaepen S, Vanderleyden J, Remans R. Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev [Internet]. 2007; 31: 425–48. Available from: https://doi.org/10.1111/j.1574-6976.2007.00072.x |
| [16] | Drake JE, Darby BA, Giasson M-A, Kramer MA, Phillips RP, Finzi AC. Stoichiometry constrains microbial response to root exudation- insights from a model and a field experiment in a temperate forest. Biogeosciences [Internet]. 2013 [cited 2022 Apr 17]; 10: 821–38. Available from: https://bg.copernicus.org/articles/10/821/2013/ |
| [17] | Patten CL, Glick BR. Role of Pseudomonas putida Indoleacetic Acid in Development of the Host Plant Root System. Appl Environ Microbiol [Internet]. American Society for Microbiology; 2002; 68: 3795–801. Available from: https://doi.org/10.1128/AEM.68.8.3795-3801.2002 |
| [18] | Sahu S, Prakash A, Shende K. Talaromyces trachyspermus, an endophyte from Withania somnifera with plant growth promoting attributes. Environ Sustain [Internet]. 2019; 2: 13–21. Available from: https://doi.org/10.1007/s42398-019-00045-5 |
| [19] | Patten CL, Glick BR. Bacterial biosynthesis of indole-3-acetic acid. Can J Microbiol. 1996; 42: 207–20. |
| [20] | Gordon SA, Weber RP. Colorimetric Estimation of Indole Acetic Acid. Plant Physiol.pg./ml.,. 1950; 192–5. Available from: https://pubmed.ncbi.nlm.nih.gov/16654351 |
| [21] | Harikrishnan H, Shanmugaiah V, Balasubramanian N. Optimization for production of Indole acetic acid (IAA) by plant growth promoting Streptomyces sp VSMGT1014 isolated from rice rhizosphere. Int J Curr Microbiol Appl Sci. 2014; 3: 158–71. |
| [22] | Mohite B. Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. J Soil Sci Plant Nutr. 2013; 13: 638–49. |
| [23] | Seyis Bilkay I, Karakoç Ş, Aksöz N. Indole-3-Acetic Acid and gibberellic acid production in Aspergillus niger Turkish J Biol. 2010; 34: 313–8. |
| [24] | Sun PF, Fang WT, Shin LY, Wei JY, Fu SF, Chou JY. Indole-3-acetic acid-producing yeasts in the phyllosphere of the carnivorous plant Drosera indica L. PLoS One. 2014; 9: 1–22. |
| [25] | Napitupulu TP, Kanti A, Sudiana IM. Evaluation of the Environmental Factors Modulating Indole-3-acetic Acid (IAA) Production by Trichoderma harzianum InaCC F88. IOP Conf Ser Earth Environ Sci. 2019; 308. |
| [26] | De A Scarcella AS, Junior RB, Bastos RG, Magri MMR. Temperature, pH and carbon source, affect drastically indole acetic acid production of plant growth promoting yeasts. Brazilian J Chem Eng. 2017; 34: 429–38. |
| [27] | Krause K, Henke C, Asiimwe T, Ulbricht A, Klemmer S, Schachtschabel D, et al. Biosynthesis and secretion of indole-3-acetic acid and its morphological effects on Tricholoma vaccinum-spruce ectomycorrhiza. Appl Environ Microbiol. 2015; 81: 7003–11. |
| [28] | Ozdal M, Ozdal OG, Sezen A, Algur OF, Kurbanoglu EB. Continuous production of indole-3-acetic acid by immobilized cells of Arthrobacter agilis. 3 Biotech [Internet]. 2017; 7: 23. Available from: https://doi.org/10.1007/s13205-017-0605-0 |
| [29] | Abusham RA, Rahman RNZRA, Salleh A, Basri M. Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand. Microb Cell Fact. 2009; 8: 1–9. |
| [30] | Chandra S, Askari K, Kumari M. Optimization of indole acetic acid production by isolated bacteria from Stevia rebaudiana rhizosphere and its effects on plant growth. J Genet Eng Biotechnol. Academy of Scientific Research & Technology; 2018; 16: 581–6. |
| [31] | Jasim B, Joseph AA, John CJ, Mathew J, Radhakrishnan EK. Isolation and characterization of plant growth promoting endophytic bacteria from the rhizome of Zingiber officinale. 3 Biotech. 2014; 4: 197–204. |
| [32] | Saber WIA, Ghoneem KM, Rashad YM, Al-Askar AA. Trichoderma Harzianum WKY1: an indole acetic acid producer for growth improvement and anthracnose disease control in sorghum. Biocontrol Sci Technol [Internet]. Taylor & Francis; 2017; 27: 654–76. Available from: https://doi.org/10.1080/09583157.2017.1321733 |
| [33] | Basu PS, Ghosh AC. Production of Indole Acetic Acid in Culture by a Rhizobium Species from the Root Nodules of a Monocotyledonous Tree, Roystonea regia. Acta Biotechnol [Internet]. John Wiley & Sons, Ltd; 2001; 21: 65–72. Available from: https://doi.org/10.1002/1521-3846(200102)21:1%3C65::AID-ABIO65%3E3.0.CO |
| [34] | Mandal S, Mandal M, Das A, Pati B, Ghosh A. Stimulation of indoleacetic acid production in a Rhizobium isolate of Vigna mungo by root nodule phenolic acids. Arch Microbiol. 2009; 191: 389–93. |
| [35] | Shokri D, Emtiazi G. Indole-3-acetic acid (IAA) production in symbiotic and non-symbiotic nitrogen-fixing bacteria and its optimization by taguchi design. Curr Microbiol. 2010; 61: 217–25. |
| [36] | Ham S, Yoon H, Park JM, Park YG. Optimization of Fermentation Medium for Indole Acetic Acid Production by Pseudarthrobacter sp. NIBRBAC000502770. Appl Biochem Biotechnol. 2021; 193: 2567–79. |
| [37] | Bhutani N, Maheshwari R, Negi M, Suneja P. Optimization of IAA production by endophytic Bacillus spp. from Vigna radiata for their potential use as plant growth promoters. Isr J Plant Sci [Internet]. Leiden, The Netherlands: Brill; 2018; 65: 83–96. Available from: https://brill.com/view/journals/ijps/65/1-2/article-p83_9.xml |
APA Style
Smriti Chouhan, Lalit Kumar Chaurasia, Anil Prakash. (2022). Optimization and Characterization of Indole Acetic Acid Producing Efficiency of Talaromyces trachyspermus for Sustainable Agro-practices. Frontiers in Environmental Microbiology, 8(2), 25-34. https://doi.org/10.11648/j.fem.20220802.12
ACS Style
Smriti Chouhan; Lalit Kumar Chaurasia; Anil Prakash. Optimization and Characterization of Indole Acetic Acid Producing Efficiency of Talaromyces trachyspermus for Sustainable Agro-practices. Front. Environ. Microbiol. 2022, 8(2), 25-34. doi: 10.11648/j.fem.20220802.12
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Download@article{10.11648/j.fem.20220802.12,
author = {Smriti Chouhan and Lalit Kumar Chaurasia and Anil Prakash},
title = {Optimization and Characterization of Indole Acetic Acid Producing Efficiency of Talaromyces trachyspermus for Sustainable Agro-practices},
journal = {Frontiers in Environmental Microbiology},
volume = {8},
number = {2},
pages = {25-34},
doi = {10.11648/j.fem.20220802.12},
url = {https://doi.org/10.11648/j.fem.20220802.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20220802.12},
abstract = {The production of indole acetic acid (IAA) by plant growth-promoting microorganisms is one of the most important factors for plant growth. Present research work deals with the characterization and optimization of different physiological conditions for IAA production by the fungus Talaromyces trachyspermus. Different environmental factors and medium components were optimized for the production of IAA by fungal culture. Effect of pH, temperature, aeration and concentration of precursor i.e. L-Tryptophan was evaluated on the biomass of fungus along with the IAA production. This work also focused on the effect of different carbon and nitrogen sources in the media for the growth of Talaromyces trachyspermus and IAA production The maximum production of IAA was observed in 15 days of incubation under the condition of 6.5 pH, 28°C temperature, and 140 RPM, in presence of 0.5% of tryptophan, 4% glucose and 0.1% sodium nitrate. After optimization, the yield of IAA by fungal culture was increased up to 0.78 fold as compared to initial production. IAA production by fungal culture was confirmed by thin-layer chromatography and HPLC analysis. Optimization of IAA production by Talaromyces trachyspermus can be useful for the large-scale production of agriculturally important bioactive metabolites. Moreover, the plant growth-promoting efficiency of this fungal culture makes it novel bio-inoculants for sustainable agriculture.},
year = {2022}
}
TY - JOUR T1 - Optimization and Characterization of Indole Acetic Acid Producing Efficiency of Talaromyces trachyspermus for Sustainable Agro-practices AU - Smriti Chouhan AU - Lalit Kumar Chaurasia AU - Anil Prakash Y1 - 2022/05/24 PY - 2022 N1 - https://doi.org/10.11648/j.fem.20220802.12 DO - 10.11648/j.fem.20220802.12 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 25 EP - 34 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20220802.12 AB - The production of indole acetic acid (IAA) by plant growth-promoting microorganisms is one of the most important factors for plant growth. Present research work deals with the characterization and optimization of different physiological conditions for IAA production by the fungus Talaromyces trachyspermus. Different environmental factors and medium components were optimized for the production of IAA by fungal culture. Effect of pH, temperature, aeration and concentration of precursor i.e. L-Tryptophan was evaluated on the biomass of fungus along with the IAA production. This work also focused on the effect of different carbon and nitrogen sources in the media for the growth of Talaromyces trachyspermus and IAA production The maximum production of IAA was observed in 15 days of incubation under the condition of 6.5 pH, 28°C temperature, and 140 RPM, in presence of 0.5% of tryptophan, 4% glucose and 0.1% sodium nitrate. After optimization, the yield of IAA by fungal culture was increased up to 0.78 fold as compared to initial production. IAA production by fungal culture was confirmed by thin-layer chromatography and HPLC analysis. Optimization of IAA production by Talaromyces trachyspermus can be useful for the large-scale production of agriculturally important bioactive metabolites. Moreover, the plant growth-promoting efficiency of this fungal culture makes it novel bio-inoculants for sustainable agriculture. VL - 8 IS - 2 ER -
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