Pneumonic pasteurellosis causes a significant economic loss in small ruminant production but its control is complicated because of drug resistance development. It is multifactorial but the most frequently isolated bacterial species are Pasteurella multocida (P. multocida), Mannheimia haemolytica (M. haemolytica), and Bibersteinia trehalosi (B. trehalosi). The antimicrobial susceptibility patterns of those commonly isolated respiratory pathogens of sheep and goats were determined to get insight for better antimicrobial therapy. The isolates were identified from pneumonic sheep and goats by Assosa Agricultural Research Center from November 2019 to April 2020 in Assosa and Bambasi woredas of Benishangul-Gumuz Regional State. 25 isolates from pneumonic sheep and 11 isolates from pneumonic goats preserved at Assosa Regional Veterinary Laboratory were tested against commonly used antimicrobial agents. Antimicrobial susceptibility profiles of the isolates were carried out using the disc diffusion method. The identified isolates were susceptible to Sulfonamide (94.4%) and Norfloxacin (91.7%). However, they were resistant to Streptomycin (88.9%) and Kanamycin (80.6%), and intermediate to Trimethoprim (55.6%). From all of the isolates, 42.4% of sheep and 40% of goat origin were resistant to all antimicrobial discs tested. About 31 out of 36 (86.6%) isolates were resistant to two or more discs and 69.4% of them were resistant to Streptomycin and Kanamycin. 81.8% (9/11) from goats and 64% (16/25) isolates from sheep were resistant to Streptomycin and Kanamycin. Eight out of thirty-six (22.2%) were resistant to three discs and none of them were resistant to four or more discs tested. Therefore, antimicrobial susceptibility monitoring programs for P. multocida, M. haemolytica, and B. trehalosi are crucial for proper antimicrobial therapy. Sulfonamide and Norfloxacin were preferred from the antimicrobial discs tested and are suggested to treat pneumonic sheep and goats. Further characterization of the isolates through a more specific diagnostic marker is recommended.
| Published in | Frontiers in Environmental Microbiology (Volume 8, Issue 2) |
| DOI | 10.11648/j.fem.20220802.11 |
| Page(s) | 19-24 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Antimicrobial Susceptibility, Bibersteinia Trehalosi, Mannheimia Haemolytica, Pasteurella multocida, Small Ruminants
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APA Style
Dessie Abera. (2022). Antimicrobial Susceptibility Patterns of Pasteurella multocida, Mannhemia Haemolytica, and Bibersteina Trehalosi Bacterial Isolates. Frontiers in Environmental Microbiology, 8(2), 19-24. https://doi.org/10.11648/j.fem.20220802.11
ACS Style
Dessie Abera. Antimicrobial Susceptibility Patterns of Pasteurella multocida, Mannhemia Haemolytica, and Bibersteina Trehalosi Bacterial Isolates. Front. Environ. Microbiol. 2022, 8(2), 19-24. doi: 10.11648/j.fem.20220802.11
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Download@article{10.11648/j.fem.20220802.11,
author = {Dessie Abera},
title = {Antimicrobial Susceptibility Patterns of Pasteurella multocida, Mannhemia Haemolytica, and Bibersteina Trehalosi Bacterial Isolates},
journal = {Frontiers in Environmental Microbiology},
volume = {8},
number = {2},
pages = {19-24},
doi = {10.11648/j.fem.20220802.11},
url = {https://doi.org/10.11648/j.fem.20220802.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20220802.11},
abstract = {Pneumonic pasteurellosis causes a significant economic loss in small ruminant production but its control is complicated because of drug resistance development. It is multifactorial but the most frequently isolated bacterial species are Pasteurella multocida (P. multocida), Mannheimia haemolytica (M. haemolytica), and Bibersteinia trehalosi (B. trehalosi). The antimicrobial susceptibility patterns of those commonly isolated respiratory pathogens of sheep and goats were determined to get insight for better antimicrobial therapy. The isolates were identified from pneumonic sheep and goats by Assosa Agricultural Research Center from November 2019 to April 2020 in Assosa and Bambasi woredas of Benishangul-Gumuz Regional State. 25 isolates from pneumonic sheep and 11 isolates from pneumonic goats preserved at Assosa Regional Veterinary Laboratory were tested against commonly used antimicrobial agents. Antimicrobial susceptibility profiles of the isolates were carried out using the disc diffusion method. The identified isolates were susceptible to Sulfonamide (94.4%) and Norfloxacin (91.7%). However, they were resistant to Streptomycin (88.9%) and Kanamycin (80.6%), and intermediate to Trimethoprim (55.6%). From all of the isolates, 42.4% of sheep and 40% of goat origin were resistant to all antimicrobial discs tested. About 31 out of 36 (86.6%) isolates were resistant to two or more discs and 69.4% of them were resistant to Streptomycin and Kanamycin. 81.8% (9/11) from goats and 64% (16/25) isolates from sheep were resistant to Streptomycin and Kanamycin. Eight out of thirty-six (22.2%) were resistant to three discs and none of them were resistant to four or more discs tested. Therefore, antimicrobial susceptibility monitoring programs for P. multocida, M. haemolytica, and B. trehalosi are crucial for proper antimicrobial therapy. Sulfonamide and Norfloxacin were preferred from the antimicrobial discs tested and are suggested to treat pneumonic sheep and goats. Further characterization of the isolates through a more specific diagnostic marker is recommended.},
year = {2022}
}
TY - JOUR T1 - Antimicrobial Susceptibility Patterns of Pasteurella multocida, Mannhemia Haemolytica, and Bibersteina Trehalosi Bacterial Isolates AU - Dessie Abera Y1 - 2022/05/24 PY - 2022 N1 - https://doi.org/10.11648/j.fem.20220802.11 DO - 10.11648/j.fem.20220802.11 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 19 EP - 24 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20220802.11 AB - Pneumonic pasteurellosis causes a significant economic loss in small ruminant production but its control is complicated because of drug resistance development. It is multifactorial but the most frequently isolated bacterial species are Pasteurella multocida (P. multocida), Mannheimia haemolytica (M. haemolytica), and Bibersteinia trehalosi (B. trehalosi). The antimicrobial susceptibility patterns of those commonly isolated respiratory pathogens of sheep and goats were determined to get insight for better antimicrobial therapy. The isolates were identified from pneumonic sheep and goats by Assosa Agricultural Research Center from November 2019 to April 2020 in Assosa and Bambasi woredas of Benishangul-Gumuz Regional State. 25 isolates from pneumonic sheep and 11 isolates from pneumonic goats preserved at Assosa Regional Veterinary Laboratory were tested against commonly used antimicrobial agents. Antimicrobial susceptibility profiles of the isolates were carried out using the disc diffusion method. The identified isolates were susceptible to Sulfonamide (94.4%) and Norfloxacin (91.7%). However, they were resistant to Streptomycin (88.9%) and Kanamycin (80.6%), and intermediate to Trimethoprim (55.6%). From all of the isolates, 42.4% of sheep and 40% of goat origin were resistant to all antimicrobial discs tested. About 31 out of 36 (86.6%) isolates were resistant to two or more discs and 69.4% of them were resistant to Streptomycin and Kanamycin. 81.8% (9/11) from goats and 64% (16/25) isolates from sheep were resistant to Streptomycin and Kanamycin. Eight out of thirty-six (22.2%) were resistant to three discs and none of them were resistant to four or more discs tested. Therefore, antimicrobial susceptibility monitoring programs for P. multocida, M. haemolytica, and B. trehalosi are crucial for proper antimicrobial therapy. Sulfonamide and Norfloxacin were preferred from the antimicrobial discs tested and are suggested to treat pneumonic sheep and goats. Further characterization of the isolates through a more specific diagnostic marker is recommended. VL - 8 IS - 2 ER -
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