Nanobiotechnology is a promising area to cater human life. Biological methods for the synthesis of silver nanoparticles are relatively cost effective process. It was aimed at synthesizing silver nanoparticles using water and soil borne bacterial isolates, characterization of silver nanoparticles using UV-Vis spectroscopy and Fourier transform infra red (FTIR) spectroscopy and its effect on multi drug resistant bacterial isolate. The biosynthesis of silver nanoparticles was evaluated in both bacterial biomass and culture supernatant of Bacillus, Pseudomonas and Escherichia coli in the presence of 1mM of AgNO3.On the basis of physical appearances of silver nano particles, Pseudomonas sp. was selected for synthesis. The absorbance spectra of reaction mixture of bacterial biomass and supernatant show the strong peak at 420 nm, indicating the presence of silver nanoparticles (AgNPs) using UV-Vis and FTIR spectrophotometry. The influence of synthesized AgNPs was tested against multi drug resistant (MDR) Staphylococcus sp. on Mueller Hinton agar. The multi antibiotics resistant Staphylococcus sp. showed antibiotic sensitivity against the antibiotic discs impregnated with silver nanoparticles. The characteristics of silver nanoparticles revealed its possible use in biomedical field.
| Published in | Frontiers in Environmental Microbiology (Volume 3, Issue 4) |
| DOI | 10.11648/j.fem.20170304.12 |
| Page(s) | 62-67 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
FTIR, MDR, Nanobiotechnology, Pseudomonas sp., Silver Nanoparticle
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APA Style
Manikant Tripathi, Anil Kumar, Shailendra Kumar. (2017). Characterization of Silver Nanoparticles Synthesizing Bacteria and Its Possible Use in Treatment of Multi Drug Resistant Isolate. Frontiers in Environmental Microbiology, 3(4), 62-67. https://doi.org/10.11648/j.fem.20170304.12
ACS Style
Manikant Tripathi; Anil Kumar; Shailendra Kumar. Characterization of Silver Nanoparticles Synthesizing Bacteria and Its Possible Use in Treatment of Multi Drug Resistant Isolate. Front. Environ. Microbiol. 2017, 3(4), 62-67. doi: 10.11648/j.fem.20170304.12
AMA Style
Manikant Tripathi, Anil Kumar, Shailendra Kumar. Characterization of Silver Nanoparticles Synthesizing Bacteria and Its Possible Use in Treatment of Multi Drug Resistant Isolate. Front Environ Microbiol. 2017;3(4):62-67. doi: 10.11648/j.fem.20170304.12
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Download@article{10.11648/j.fem.20170304.12,
author = {Manikant Tripathi and Anil Kumar and Shailendra Kumar},
title = {Characterization of Silver Nanoparticles Synthesizing Bacteria and Its Possible Use in Treatment of Multi Drug Resistant Isolate},
journal = {Frontiers in Environmental Microbiology},
volume = {3},
number = {4},
pages = {62-67},
doi = {10.11648/j.fem.20170304.12},
url = {https://doi.org/10.11648/j.fem.20170304.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20170304.12},
abstract = {Nanobiotechnology is a promising area to cater human life. Biological methods for the synthesis of silver nanoparticles are relatively cost effective process. It was aimed at synthesizing silver nanoparticles using water and soil borne bacterial isolates, characterization of silver nanoparticles using UV-Vis spectroscopy and Fourier transform infra red (FTIR) spectroscopy and its effect on multi drug resistant bacterial isolate. The biosynthesis of silver nanoparticles was evaluated in both bacterial biomass and culture supernatant of Bacillus, Pseudomonas and Escherichia coli in the presence of 1mM of AgNO3.On the basis of physical appearances of silver nano particles, Pseudomonas sp. was selected for synthesis. The absorbance spectra of reaction mixture of bacterial biomass and supernatant show the strong peak at 420 nm, indicating the presence of silver nanoparticles (AgNPs) using UV-Vis and FTIR spectrophotometry. The influence of synthesized AgNPs was tested against multi drug resistant (MDR) Staphylococcus sp. on Mueller Hinton agar. The multi antibiotics resistant Staphylococcus sp. showed antibiotic sensitivity against the antibiotic discs impregnated with silver nanoparticles. The characteristics of silver nanoparticles revealed its possible use in biomedical field.},
year = {2017}
}
TY - JOUR T1 - Characterization of Silver Nanoparticles Synthesizing Bacteria and Its Possible Use in Treatment of Multi Drug Resistant Isolate AU - Manikant Tripathi AU - Anil Kumar AU - Shailendra Kumar Y1 - 2017/11/25 PY - 2017 N1 - https://doi.org/10.11648/j.fem.20170304.12 DO - 10.11648/j.fem.20170304.12 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 62 EP - 67 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20170304.12 AB - Nanobiotechnology is a promising area to cater human life. Biological methods for the synthesis of silver nanoparticles are relatively cost effective process. It was aimed at synthesizing silver nanoparticles using water and soil borne bacterial isolates, characterization of silver nanoparticles using UV-Vis spectroscopy and Fourier transform infra red (FTIR) spectroscopy and its effect on multi drug resistant bacterial isolate. The biosynthesis of silver nanoparticles was evaluated in both bacterial biomass and culture supernatant of Bacillus, Pseudomonas and Escherichia coli in the presence of 1mM of AgNO3.On the basis of physical appearances of silver nano particles, Pseudomonas sp. was selected for synthesis. The absorbance spectra of reaction mixture of bacterial biomass and supernatant show the strong peak at 420 nm, indicating the presence of silver nanoparticles (AgNPs) using UV-Vis and FTIR spectrophotometry. The influence of synthesized AgNPs was tested against multi drug resistant (MDR) Staphylococcus sp. on Mueller Hinton agar. The multi antibiotics resistant Staphylococcus sp. showed antibiotic sensitivity against the antibiotic discs impregnated with silver nanoparticles. The characteristics of silver nanoparticles revealed its possible use in biomedical field. VL - 3 IS - 4 ER -
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