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Antimicrobial Activity of Tithonia diversifolia, Elephantopus scaber, and Kigelia africana Against Plant Pathogens

Received: 2 July 2017     Accepted: 24 August 2017     Published: 10 October 2017
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Abstract

Synthetic pesticides, one among agricultural inputs, have been used and applied to crop production, particularly during plant pathogen attacks. Although promisingly, possible effect that the application of pesticides on agro-ecosystem may have to be concerned to support health of food, consumers and to the environment. Alternatively, exploration of the potential plants that probably have natural antimicrobial compounds is important step to discover natural pesticide as component of plant disease management. Some of plants with low economical value such as Tithonia diversifolia, Elephantopus scaber, and Kigelia africana have been known to have antimicrobial substances and successfully demonstrated against food and human pathogens. These, bring us to study their potency in controlling several plant pathogens of important crops, either fungal such as Phytophthora nicotianae and Rhizoctonia solani, or bacterial pathogens such as Ralstonia solanacearum and Xanthomonas oryzae. Leave extracts of both, T. diversifolia and E. scaber, and fruit extract of K. africana were obtained and concentrated using methanol. Our results showed that all extracts contained flavonoid, tannin, and alkaloid but the amount of the content of each extract was different. Among extracts used in this study, fruit extract of K. africana was known to contain the highest flavonoid and tannin content of 21, 54 μg QE/ml and 28.95 μg GAE/ml, respectively, with low content on alkaloid (3.32 μg AE/ml) compared to other plant extracts. To test it potency as biopesticides, antimicrobial activity against fungal pathogens were evaluated using poisoned food technique method while antimicrobial activity against pathogenic bacteria were evaluated using spot test method. The result showed that extract from K. africana fruit was able to inhibit fungal pathogen R. solani, while extracts of E. scaber and T. diversifolia were have inhibition ability against P. nicotianae. In addition, the E. scaber extract was also able to inhibit bacteria R. solanacearum and X. oryzae. In average, 5 mg/ml of extracts were demonstrated to give the best performance in inhibit plant pathogens.

Published in Frontiers in Environmental Microbiology (Volume 3, Issue 4)
DOI 10.11648/j.fem.20170304.11
Page(s) 56-61
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Biopesticide, Plant Pathogen, Plant Disease, Fungal Disease, Bacterial Disease

References
[1] Akbar, M. W., Sengupta, D. and Chowdhury, A. (2009). Impact of pesticide use in agriculture: their benefits and hazards. Interdisciplinary Toxicology 2 (1): 1-2.
[2] Vincelli, P. (2016). Genetic engineering and sustainable crop disease management: opportunities for case-by-case decision-making. Sustainability 8 (5): 495.
[3] Gilligan, C. A. (2008). Sustainable agriculture and plant diseases: an epidemiological perspective. Philos. Trans. R. Soc., B, Biol. Sci. 363 (1492): 741–759.
[4] Yoon, M.-Y., Cha, B. and Kim, J.-C. (2013). Recent trends in studies on botanical fungicides in agriculture. Plant Pathol. J., 29 (1): 1–9.
[5] Copping, L. G., and Menn, J. J. (2000). Biopesticides: a review of their action, applications and efficacy. Pest Manag. Sci. 56: 651–676.
[6] Ueda, K., Kawabata, R., Irie, T., Nakai, Y., Tohya, Y. and Sakaguchi, T. (2013). Inactivation of pathogenic viruses by plant-derived tannins: strong effects of extracts from persimmon (Diospyros kaki) on a broad range of viruses. PLoS ONE 8 (1): e55343.
[7] Upadhyay, A., Upadhyaya, I., Kollanoor-Johny, A. and Venkitanarayanan, K. (2014). Combating pathogenic microorganisms using plant-derived antimicrobials: a minireview of the mechanistic basis. Bio Med Research International. 2014: 18p.
[8] Bardají, D. K. R., Reis, E. B., Medeiros, T. C. T., Lucarini, R., Crotti, A. E. M., and Martins, C. H. G. (2016). Antibacterial activity of commercially available plant-derived essential oils against oral pathogenic bacteria. Natural Product Research 30 (10):1178-1181.
[9] Daisy, P., Mathew, S., Suveena, S., and Rayan, N. A. (2008). A novel terpenoid from Elephantopus Scaber – antibacterial Activity on Staphylococcus aureus: a substantiate computational approach. International Journal of Biomedical Science  4 (3): 196–203.
[10] Dewole, J. O. O., and Oni, S. O. (2013). Phytochemical and antimicrobial studies of extracts from the leaves of Tithonia diversifolia for pharmaceutical importance. Pharmacy and Biological Sciences 6 (4): 21-23.
[11] Saini, S., Kaur, H., Verma, B., Ripudaman and Singh, S. K. (2009). Kigelia africana (Lam.) Benth. an overview. Natural Product Radiance 8 (2): 190-193.
[12] Supyani and Gutomo, H. S. (2014). Hypovirulent isolates of Rhizoctonia solani collected from rice in Karanganyar Regency, Central Java, Indonesia. ARPN Journal of Agricultural and Biological Science 9 (1):19-23.
[13] Fang, Y., Zhang, L., Jiao, Y., Liao, J., Luo, L., Ji, S., Yang, M. (2016). Tobacco Rotated with Rapeseed for Soil-Borne Phytophthora Pathogen Biocontrol: Mediated by Rapeseed Root Exudates. Frontiers in Microbiology 7: 894.
[14] Sudir, and Yuliani, D. (2016). Composition and distribution of Xanthomonas oryzae pv. oryzae pathotypes, the pathogen of rice bacterial leaf blight in Indonesia. AGRIVITA Journal of Agricultural Science 38 (2): 174-185.
[15] Addy, H. S., Azizi, N. F., and Mihardjo, P. A. (2016). Detection of bacterial wilt pathogen and isolation of its bacteriophage from banana in Lumajang Area, Indonesia. International Journal of Agronomy 2016: 7p.
[16] Jack, I. R., and Orubite, K. O. (2008). Phytochemical analysis and antimicrobial activity of the extract of leaves of Fleabane (Conyza Sumatrensis). Appl. Sci. Environ. Manage. 12 (4): 63-65.
[17] Kumar, R. S., Venkateshwar, C., Samuel, G., and Rao, S. G. (2013). Phytochemical screening of some coumpounds from plant leaf extracts of Holoptelea Integrifilia (Planch.) and Celestrus emarginata (Grah.) used by gondu tribes at Adilabad District, Andhradesh, India. Engineering Science Invention 2 (8): 66-67.
[18] Douglas, K. and Jeruto, J. (2016). Phytochemistry and antimicrobial of extract from medicinal plants Tithonia diversifolia and Olea africana. British Journal of Pharmaceutical Research 12 (3):1-3.
[19] Rao, T. M., Rao, B. G., and Rao, Y. V. (2012). Antioxidant activity of Spilanthes acmella extracts. Int. J. Phytopharmacol. 3 (2): 216-220.
[20] Singh, R., Verma, P. K., and Singh, G. (2012). Total phenolic, flavonoid and tannin contents in different extracts of Artemisia absinthium. Intercult. Enthnopharmacol. 1 (2): 101-104.
[21] Padma, S., and Deepika, S.. (2013). Phytochemical screening and in-vitro antifungal investigation of Pasrthenium Hysterophorus extracts against Alternaria alternata. International Research Journal of Pharmacy 4 (7): 190-193.
[22] Aniszewki, T. (2007). Alkaloid-Secrets of life. Elsevier: Netherlands.
[23] Fan, L., Soccol, A. T., Pandey, A., Vandenberghe, L. P. D. S. and Soccol, C. R. (2006). Effect of caffeine and tannins on cultivation and fructification of Pleurotus on coffee husks. Microbiology 37: 420-424.
[24] Cheo, P. C. (1982). Effects of tannic acid on rhizomorph production by Armilaria mellea. Physiology and Biochemistry 72 (6): 676-667.
[25] Nes, W. D., Hanners, P. K., Bean, G. A. and Patterson, G. W. 1982. Inhibition of growth and sitosterol-induced sexual reproduction in Phythophthora cactorum by steroidal alkaloids. Physiology and Biochemistry 72 (4): 447-449.
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    Desi Rejeki, Suharto, Hardian Susilo Addy. (2017). Antimicrobial Activity of Tithonia diversifolia, Elephantopus scaber, and Kigelia africana Against Plant Pathogens. Frontiers in Environmental Microbiology, 3(4), 56-61. https://doi.org/10.11648/j.fem.20170304.11

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    ACS Style

    Desi Rejeki; Suharto; Hardian Susilo Addy. Antimicrobial Activity of Tithonia diversifolia, Elephantopus scaber, and Kigelia africana Against Plant Pathogens. Front. Environ. Microbiol. 2017, 3(4), 56-61. doi: 10.11648/j.fem.20170304.11

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    AMA Style

    Desi Rejeki, Suharto, Hardian Susilo Addy. Antimicrobial Activity of Tithonia diversifolia, Elephantopus scaber, and Kigelia africana Against Plant Pathogens. Front Environ Microbiol. 2017;3(4):56-61. doi: 10.11648/j.fem.20170304.11

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  • @article{10.11648/j.fem.20170304.11,
      author = {Desi Rejeki and Suharto and Hardian Susilo Addy},
      title = {Antimicrobial Activity of Tithonia diversifolia, Elephantopus scaber, and Kigelia africana Against Plant Pathogens},
      journal = {Frontiers in Environmental Microbiology},
      volume = {3},
      number = {4},
      pages = {56-61},
      doi = {10.11648/j.fem.20170304.11},
      url = {https://doi.org/10.11648/j.fem.20170304.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20170304.11},
      abstract = {Synthetic pesticides, one among agricultural inputs, have been used and applied to crop production, particularly during plant pathogen attacks. Although promisingly, possible effect that the application of pesticides on agro-ecosystem may have to be concerned to support health of food, consumers and to the environment. Alternatively, exploration of the potential plants that probably have natural antimicrobial compounds is important step to discover natural pesticide as component of plant disease management. Some of plants with low economical value such as Tithonia diversifolia, Elephantopus scaber, and Kigelia africana have been known to have antimicrobial substances and successfully demonstrated against food and human pathogens. These, bring us to study their potency in controlling several plant pathogens of important crops, either fungal such as Phytophthora nicotianae and Rhizoctonia solani, or bacterial pathogens such as Ralstonia solanacearum and Xanthomonas oryzae. Leave extracts of both, T. diversifolia and E. scaber, and fruit extract of K. africana were obtained and concentrated using methanol. Our results showed that all extracts contained flavonoid, tannin, and alkaloid but the amount of the content of each extract was different. Among extracts used in this study, fruit extract of K. africana was known to contain the highest flavonoid and tannin content of 21, 54 μg QE/ml and 28.95 μg GAE/ml, respectively, with low content on alkaloid (3.32 μg AE/ml) compared to other plant extracts. To test it potency as biopesticides, antimicrobial activity against fungal pathogens were evaluated using poisoned food technique method while antimicrobial activity against pathogenic bacteria were evaluated using spot test method. The result showed that extract from K. africana fruit was able to inhibit fungal pathogen R. solani, while extracts of E. scaber and T. diversifolia were have inhibition ability against P. nicotianae. In addition, the E. scaber extract was also able to inhibit bacteria R. solanacearum and X. oryzae. In average, 5 mg/ml of extracts were demonstrated to give the best performance in inhibit plant pathogens.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Antimicrobial Activity of Tithonia diversifolia, Elephantopus scaber, and Kigelia africana Against Plant Pathogens
    AU  - Desi Rejeki
    AU  - Suharto
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    DO  - 10.11648/j.fem.20170304.11
    T2  - Frontiers in Environmental Microbiology
    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
    SP  - 56
    EP  - 61
    PB  - Science Publishing Group
    SN  - 2469-8067
    UR  - https://doi.org/10.11648/j.fem.20170304.11
    AB  - Synthetic pesticides, one among agricultural inputs, have been used and applied to crop production, particularly during plant pathogen attacks. Although promisingly, possible effect that the application of pesticides on agro-ecosystem may have to be concerned to support health of food, consumers and to the environment. Alternatively, exploration of the potential plants that probably have natural antimicrobial compounds is important step to discover natural pesticide as component of plant disease management. Some of plants with low economical value such as Tithonia diversifolia, Elephantopus scaber, and Kigelia africana have been known to have antimicrobial substances and successfully demonstrated against food and human pathogens. These, bring us to study their potency in controlling several plant pathogens of important crops, either fungal such as Phytophthora nicotianae and Rhizoctonia solani, or bacterial pathogens such as Ralstonia solanacearum and Xanthomonas oryzae. Leave extracts of both, T. diversifolia and E. scaber, and fruit extract of K. africana were obtained and concentrated using methanol. Our results showed that all extracts contained flavonoid, tannin, and alkaloid but the amount of the content of each extract was different. Among extracts used in this study, fruit extract of K. africana was known to contain the highest flavonoid and tannin content of 21, 54 μg QE/ml and 28.95 μg GAE/ml, respectively, with low content on alkaloid (3.32 μg AE/ml) compared to other plant extracts. To test it potency as biopesticides, antimicrobial activity against fungal pathogens were evaluated using poisoned food technique method while antimicrobial activity against pathogenic bacteria were evaluated using spot test method. The result showed that extract from K. africana fruit was able to inhibit fungal pathogen R. solani, while extracts of E. scaber and T. diversifolia were have inhibition ability against P. nicotianae. In addition, the E. scaber extract was also able to inhibit bacteria R. solanacearum and X. oryzae. In average, 5 mg/ml of extracts were demonstrated to give the best performance in inhibit plant pathogens.
    VL  - 3
    IS  - 4
    ER  - 

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Author Information
  • Agrotechnology Study Program, Faculty of Agriculture, University of Jember, Jember, Indonesia

  • Agrotechnology Study Program, Faculty of Agriculture, University of Jember, Jember, Indonesia

  • Agrotechnology Study Program, Faculty of Agriculture, University of Jember, Jember, Indonesia

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