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Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria

Received: 31 August 2015     Accepted: 13 September 2015     Published: 26 September 2015
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Abstract

This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT.

Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 5)
DOI 10.11648/j.bio.20150305.13
Page(s) 40-46
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Dibenzothiophene, Biodesulfurization, Isolation, P. aeruginosa, GC/MS

References
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[2] Al-Hassar, Z. A., 2010. Biodegradation and Biodesulfuruzation Substrate Spectrum for Dibenzothiophene-Desulfurizing Bacteria. M. sc. Thesis. College of graduate studies- Arabian Gulf University.
[3] Alves, L., Paixão, S. M., 2011. Toxicity Evaluation of 2-Hydroxybiphenyl and Other Compounds Involved in Studies of Fossil Fuels Biodesulphurisation. J. Bioresour. Technol., 102: 9162–9166.
[4] Chen, H., Cai, YB., Zhang, W. J., Li, W., 2009. Methoxylation pathway in biodesulfurization of model organosulfur compounds with Mycobacterium sp. J. Bioresour. Technol.; 100: 2085–2087.
[5] Gao, L. D., Tang, Y., Xue, Q. S., Liu, Y., Lu, Y., 2009. Hydrotalcite-like compounds derived Cu Zn Al oxide catalysts for aerobic oxidative removal of gasoline-range organosulfur compounds. Energy Fuels. 23, 624–630.
[6] Gupta, N., Roychoudhury, P. K., Deb, J. K., 2005. Biotechnology of desulfurization of diesel: Prospects and challenges. Appl. Microbiol. Biotechnol. 66: 356–366.
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[8] Ichinose, H., Wariishi, H., Tanaka, H., 1999. Bioconversion of recalcitrant 4-methyldibenzothiophene to water-extractable products using lignin-degrading basidiomycete Coriolus versicolor. Biotechnol. Prog. 15: 706-714.
[9] Kilbane, J. J., 2006. Microbial biocatalyst development to upgrade fossil fuels. Curr Opin Biotechnol 17, 305–314.
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[11] Liu, H., Yu, J., Bao, X., 2007. The State-of-the-art and future perspectives of world petroleum refining technology [J]. The Chinese Journal of Process Engineering. 7 (1): 176 -185.
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[13] Mandal, A. J., Sarma, P. M., Singh, B., Jeyaseelan, C. P., Channashettar, V. A., 2012. Bioremediation: An environment friendly, sustainable biotechnological solution for remediation of petroleum hydrocarbon contaminated waste. ARPN Journal of Science and Technology 2 (Special Issue): 1-12.
[14] Mohebali, G., Ball, A. S., 2008. Biocatalytic desulfurization (BDS) of petrodiesel fuels. Microbiology 154:2169–2183.
[15] Mohebali, G., Ball, A., Rasekh, B., Kaytash, A., 2007. Biodesulfurization potential of a newly isolated bacterium, Gordonia alkanivorans RIPI190A. Enzyme and Microbial Technol., 40: 578-584.
[16] Monticello, D. J., 2000. Biodesulfurization and the upgrading of petroleum distillates. Current Opinion in Biotechnology, 11, 540-546.
[17] Oldfield, C., Pogrebinsky, O., Simmonds, J., Olson, E. S., Kulpa, C., 1997. Elucidation of the metabolic pathway for dibenzothiophene desulphurization by Rhodococcus sp. strain IGTS8 (ATCC 53968). Microbiology 143: 2961–2973.
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Cite This Article
  • APA Style

    Majid Hussein Al-Jailawi, Albab Fawaz Al-Faraas, Abdelghani Ibrahem Yahia. (2015). Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria. American Journal of Bioscience and Bioengineering, 3(5), 40-46. https://doi.org/10.11648/j.bio.20150305.13

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    ACS Style

    Majid Hussein Al-Jailawi; Albab Fawaz Al-Faraas; Abdelghani Ibrahem Yahia. Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria. Am. J. BioSci. Bioeng. 2015, 3(5), 40-46. doi: 10.11648/j.bio.20150305.13

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    AMA Style

    Majid Hussein Al-Jailawi, Albab Fawaz Al-Faraas, Abdelghani Ibrahem Yahia. Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria. Am J BioSci Bioeng. 2015;3(5):40-46. doi: 10.11648/j.bio.20150305.13

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  • @article{10.11648/j.bio.20150305.13,
      author = {Majid Hussein Al-Jailawi and Albab Fawaz Al-Faraas and Abdelghani Ibrahem Yahia},
      title = {Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {3},
      number = {5},
      pages = {40-46},
      doi = {10.11648/j.bio.20150305.13},
      url = {https://doi.org/10.11648/j.bio.20150305.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150305.13},
      abstract = {This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria
    AU  - Majid Hussein Al-Jailawi
    AU  - Albab Fawaz Al-Faraas
    AU  - Abdelghani Ibrahem Yahia
    Y1  - 2015/09/26
    PY  - 2015
    N1  - https://doi.org/10.11648/j.bio.20150305.13
    DO  - 10.11648/j.bio.20150305.13
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
    SP  - 40
    EP  - 46
    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.20150305.13
    AB  - This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT.
    VL  - 3
    IS  - 5
    ER  - 

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Author Information
  • Department of Molecular & Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq

  • Department of Biotechnology, College of Science, Al-Nahrain University, Baghdad, Iraq

  • Department of Molecular & Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq

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