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Evaluation of Anti-ulcerogenic Activity in Oil Extract of Jintan Hitam (Nigella sativa) Against Ethanol Induced Gastric Ulcer in Mice (Mus musculus)

Received: 3 October 2016     Accepted: 22 October 2016     Published: 14 November 2016
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Abstract

Jintan hitam (Nigella sativa) has been widely used in traditional medicine to treat several diseases, including gastric ulcer. However, there are no scientific data demonstrating anti-ulcerogenic activity conferred by use of jintan hitam. The present study aimed to evaluate the antiulcer properties of oil extract from jintan hitam. In the gastric ulcer induced by administration of 80% ethanol model, observation made for ulcer score and index, level of ulcer erosion, total amount of polymorphonuclear cells and morphological change of 80% ethanol induced gastric ulcer of mice. Research was conducted on laboratory in Faculty of Medicine Universitas Hasanuddin from April to September 2015, with post test only experimental group design. There were 40 mice used in this research, divided into five groups, each group consisted of 8 mice: negative control group, positive control group, and three different doses of oil extract (0.1, 0.2 and 0.3 ml per 20g bw of mice). Observation made for 4 and 7 days. The oil extract of jintan hitam showed significant differences on doses of 0.1 ml per 20g bw of mice given in 4 days, with p = 0.034 (p<0.05) based on score and index of gastric ulcer.

Published in American Journal of Clinical and Experimental Medicine (Volume 4, Issue 6)
DOI 10.11648/j.ajcem.20160406.14
Page(s) 179-184
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Oil Extract of Jintan Hitam, Ulcer Score, Ulcer Index, Ulcer Erosion, Polymorphonuclear Cells, Morphological Change of Gastric

References
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Cite This Article
  • APA Style

    Syamsu Rijal, Upik Anderiani Miskad, Djumadi Achmad, Rina Masadah, Dasril Daud, et al. (2016). Evaluation of Anti-ulcerogenic Activity in Oil Extract of Jintan Hitam (Nigella sativa) Against Ethanol Induced Gastric Ulcer in Mice (Mus musculus). American Journal of Clinical and Experimental Medicine, 4(6), 179-184. https://doi.org/10.11648/j.ajcem.20160406.14

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    ACS Style

    Syamsu Rijal; Upik Anderiani Miskad; Djumadi Achmad; Rina Masadah; Dasril Daud, et al. Evaluation of Anti-ulcerogenic Activity in Oil Extract of Jintan Hitam (Nigella sativa) Against Ethanol Induced Gastric Ulcer in Mice (Mus musculus). Am. J. Clin. Exp. Med. 2016, 4(6), 179-184. doi: 10.11648/j.ajcem.20160406.14

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    AMA Style

    Syamsu Rijal, Upik Anderiani Miskad, Djumadi Achmad, Rina Masadah, Dasril Daud, et al. Evaluation of Anti-ulcerogenic Activity in Oil Extract of Jintan Hitam (Nigella sativa) Against Ethanol Induced Gastric Ulcer in Mice (Mus musculus). Am J Clin Exp Med. 2016;4(6):179-184. doi: 10.11648/j.ajcem.20160406.14

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  • @article{10.11648/j.ajcem.20160406.14,
      author = {Syamsu Rijal and Upik Anderiani Miskad and Djumadi Achmad and Rina Masadah and Dasril Daud and Cahyono Kaelan and Halida Rahawarin and Swandari Paramita and Yadi Yasir},
      title = {Evaluation of Anti-ulcerogenic Activity in Oil Extract of Jintan Hitam (Nigella sativa) Against Ethanol Induced Gastric Ulcer in Mice (Mus musculus)},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {4},
      number = {6},
      pages = {179-184},
      doi = {10.11648/j.ajcem.20160406.14},
      url = {https://doi.org/10.11648/j.ajcem.20160406.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20160406.14},
      abstract = {Jintan hitam (Nigella sativa) has been widely used in traditional medicine to treat several diseases, including gastric ulcer. However, there are no scientific data demonstrating anti-ulcerogenic activity conferred by use of jintan hitam. The present study aimed to evaluate the antiulcer properties of oil extract from jintan hitam. In the gastric ulcer induced by administration of 80% ethanol model, observation made for ulcer score and index, level of ulcer erosion, total amount of polymorphonuclear cells and morphological change of 80% ethanol induced gastric ulcer of mice. Research was conducted on laboratory in Faculty of Medicine Universitas Hasanuddin from April to September 2015, with post test only experimental group design. There were 40 mice used in this research, divided into five groups, each group consisted of 8 mice: negative control group, positive control group, and three different doses of oil extract (0.1, 0.2 and 0.3 ml per 20g bw of mice). Observation made for 4 and 7 days. The oil extract of jintan hitam showed significant differences on doses of 0.1 ml per 20g bw of mice given in 4 days, with p = 0.034 (p<0.05) based on score and index of gastric ulcer.},
     year = {2016}
    }
    

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    T1  - Evaluation of Anti-ulcerogenic Activity in Oil Extract of Jintan Hitam (Nigella sativa) Against Ethanol Induced Gastric Ulcer in Mice (Mus musculus)
    AU  - Syamsu Rijal
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    AU  - Halida Rahawarin
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    AU  - Yadi Yasir
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    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
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    AB  - Jintan hitam (Nigella sativa) has been widely used in traditional medicine to treat several diseases, including gastric ulcer. However, there are no scientific data demonstrating anti-ulcerogenic activity conferred by use of jintan hitam. The present study aimed to evaluate the antiulcer properties of oil extract from jintan hitam. In the gastric ulcer induced by administration of 80% ethanol model, observation made for ulcer score and index, level of ulcer erosion, total amount of polymorphonuclear cells and morphological change of 80% ethanol induced gastric ulcer of mice. Research was conducted on laboratory in Faculty of Medicine Universitas Hasanuddin from April to September 2015, with post test only experimental group design. There were 40 mice used in this research, divided into five groups, each group consisted of 8 mice: negative control group, positive control group, and three different doses of oil extract (0.1, 0.2 and 0.3 ml per 20g bw of mice). Observation made for 4 and 7 days. The oil extract of jintan hitam showed significant differences on doses of 0.1 ml per 20g bw of mice given in 4 days, with p = 0.034 (p<0.05) based on score and index of gastric ulcer.
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Author Information
  • Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

  • Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

  • Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

  • Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

  • Departement of Pediatric, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

  • Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

  • Departement of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

  • Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia

  • Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia

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