| Peer-Reviewed

In Vitro and in Vivo Antioxidant Related Effects of Rosemary (Rosmarinus Officinalis L.) Extracts in Humans

Received: 22 August 2015     Accepted: 6 September 2015     Published: 16 September 2015
Views:       Downloads:
Abstract

Rosemary (Rosmarinus officinalis L.) belongs to the family Lamiaceae (Labiatae) is a well-known aromatic plant used all around the world for different medicinal purposes. Aims: The present work aimed to investigate antioxidant related effects of rosemary in humans by in vitro and in vivo studies. Study design: Chemical antioxidant assays and venous blood from healthy volunteers were used to conduct in vitro experiments. For in vivo study, nine healthy volunteers, each received orally 250 ml of aqueous extract of rosemary daily for 5 days. Venous bloods were taken before and one hour after the first dose of aqueous extract (sample I and II respectively) and then one day after the last dose of day five (i.e. day 6, sample III). The first blood taken before the first dose (i.e. sample I), served as control for the next samples of II and III. Methodology: The following assays were performed: total antioxidant capacity (TAC), reducing power, scavenging activity of DPPH, scavenging activity of OH, iron chelating ability, erythrocyte reduced glutathione (GSH), malonyldialdehyde (MDA), protein carbonyl (PC), superoxide dismutase (SOD), percentage hemolysis, serum total antioxidant status (TAS) and serum selected biochemical tests. Results: Rosemary extract showed efficient total antioxidant capacity, reducing power, scavenging activity of DPPH, scavenging activity of OH and a reasonable iron chelating ability. Pre-incubation of erythrocytes in vitro with methanolic extract of rosemary then exposed to H₂O₂ decreased significantly MDA production (i.e. anti-lipid-peroxidant), PC production (i.e. anti-protein-oxidant) and oxidant hemolysis (i.e. anti-hemolytic) in a concentration dependent manner. Oral administration of aqueous extracts of rosemary to healthy volunteers, for 5 days, increased significantly serum TAS (18% increase), erythrocyte GSH (72% increase), erythrocyte superoxide dismutase (SOD) (21% increase) and decreased significantly erythrocyte MDA (17% decrease), with no effect on serum biochemical tests for kidney, liver, cardiac and pancreatic, compared to 0 time administration. Conclusion: Rosemary extracts have efficient in vitro and in vivo antioxidant related effects. As the present findings are obtained in healthy humans with no oxidative stress, this indicates that rosemary can improve the base line of the defense mechanisms against possible oxidative stress, with no adverse effects, thus decreasing susceptibility or preventing the progress of pathological conditions related to oxidative stress.

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 5)
DOI 10.11648/j.ajcem.20150305.13
Page(s) 213-221
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Rosemary, Total Antioxidant Status, Lipid Peroxidation, Protein Carbonyl, MDA, GSH, Superoxide Dismutase, Medicinal Plants, Serum Biochemical Tests, in Vitro, in Vivo

References
[1] Al-Sereiti MR, Abu-Amer KM and Sen P: Pharmacology of rosemary (Rosmarinus officinalis Linn.) and its therapeutic potentials. Indian J Exp Biol 1999; 37: 124–130.
[2] Assessment report on Rosmarinus officinalis L., aetheroleum and Rosmarinus officinalis L., folium EMA/HMPC/13631/2009. 15 July 2010.
[3] Faixová, Z. and Faix, Š: Biological effects of rosemary (Rosmarinus offficinalis L.) essential oil (A Review). FOLIA VETERINARIA 2008; 52(3-4): 135-39.
[4] Rocha, J., Eduardo-Figueira, M., Barateiro, A., Fernandes, A., Brites, D., Bronze, R., Duarte, C. M., Serra, A. T., Pinto, R., Freitas, M., Fernandes, E., Silva-Lima, B., Mota-Filipe, H. and Sepodes, B: Anti-inflammatory Effect of Rosmarinic Acid and an Extract of Rosmarinus officinalis in Rat Models of Local and Systemic Inflammation. Basic & Clinical Pharmacology & Toxicology 2015; 116:398–413.
[5] Borhan Shokrollahi, Fardin Amini, Shahin Fakour, Mohammad Amiri Andi: Effect of rosemary (Rosmarinus officinalis) extract on weight, hematology and cell-mediated immune response of newborn goat kids. Journal of Agriculture and Rural Development in the Tropics and Subtropics 2015; 116(1): 91–97.
[6] Eilyad Issabeagloo, Parviz Kermanizadeh, Mohammad Taghizadieh and Reza Forughi: Antimicrobial effects of rosemary (Rosmarinus officinalis L.) essential oils against Staphylococcus spp. African Journal of Microbiology Research 2012; 6(23): 5039-42.
[7] Ngo SN, Williams DB and Head RJ: Rosemary and cancer prevention: preclinical perspectives. Crit Rev Food Sci Nutr 2011; 51: 946–54.
[8] O. Santana-Méridas, M. Polissiou, M. E. Izquierdo-Melero, K. Astraka, Petros A. Tarantilis, D. Herraiz-Peñalver and R. Sánchez-Vioque: Polyphenol composition, antioxidant and bioplaguicide activities of the solid residue from hydrodistillation of Rosmarinus officinalis L. Industrial Crops and Products 2014; 59: 125–34.
[9] Pérez-Fons L, Garzón MT and Micol V: Relationship between the antioxidant capacity and effect of rosemary (Rosmarinus officinalis L.) polyphenols on membrane phospholipid order. J Agric Food Chem 2010; 58(1): 161-71.
[10] Maria J. Jordán, Vanesa Lax, Maria C. Rota, Susana Lorán, and José A. Sotomayor: Relevance of Carnosic Acid, Carnosol, and Rosmarinic Acid Concentrations in the in Vitro Antioxidant and Antimicrobial Activities of Rosmarinus officinalis (L.) Methanolic Extracts. J. Agric. Food Chem 2012; 60 (38): 9603–8.
[11] Mayara Fumiere Lemos, Mariana Fumiere Lemos, Henrique Poltronieri Pacheco, Denise Coutinho Endringer and Rodrigo Scherer: Seasonality modifies rosemary’s composition and biological activity. Industrial Crops and Products 2015; 70: 41–47.
[12] Urszula Gawlik-Dziki and Michał Świeca: Effect of various pH conditions simulated in vivo on the activity of lipophilic antioxidants isolated from selected spices. Pol. J. Food Nutr. Sci. 2007; 57(3A): 19-22.
[13] Dorman H. J. D., Peltoketo A., Hiltunen R and Tikkanen M. J: Characterization of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chem 2003; 83: 255-62.
[14] Viuda-Martos M, Ruiz Navajas Y, Sánchez Zapata E, Fernández-López J, and Pérez-Álvarez JA: Antioxidant activity of essential oils of five spice plants widely used in a Mediterranean diet. Flavour Frag J. 2010; 25: 13–19.
[15] Sahar, T. Ahmad; Abeer, S. Hafez; Manal, A. Hassan and Mogda, K. Mansour: Influence of Rosemary Extract on Immune Responses and Oxidative Stress in Mice Intoxicated by Aflatoxins. Nature and Science 2011; 9(10): 54-63.
[16] Aleksandar Rašković, Isidora Milanović, Nebojša Pavlović, Tatjana Ćebović, Saša Vukmirović and Momir Miko: Antioxidant activity of rosemary (Rosmarinus officinalis L.) essential oil and its hepatoprotective potential. BMC Complementary and Alternative Medicine 2014; 14:225.
[17] Manal A Emam: Comparative evaluation of antidiabetic activity of Rosmarinus officinalis L. and Chamomile recutita in streptozotocin induced diabetic rats. Agric. Biol. J. N. Am. 2012; 3(6): 247-52.
[18] Olfat A. Khalil, Kholoud S. Ramadan, Enas N. Danial, Hanan S. Alnahdi and Najla O. Ayaz: Antidiabetic activity of Rosmarinus officinalis and its relationship with the antioxidant property. African Journal of Pharmacy and Pharmacology 2012; 6(14):1031-36.
[19] Bakirel T, Bakirel U, Keleş OU, Ulgen SG and Yardibi H: In vivo assessment of antidiabetic and antioxidant activities of rosemary (Rosmarinus officinalis) in Alloxan-Diabetic Rabbits. Journal of Ethnopharmacology 2008; 116: 64-73.
[20] Packer, L, Colman, C. The Antioxidant Miracle. Canada. John Wiley & Sons Inc. Pub. 1999.
[21] Valko, M, Leibfritz, D, Moncol, J, Cronin, MT, Mazur, M and Telser, J: Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 2007; 39:44-84.
[22] Abdulrahim Al Jamal: Effect of rosemary (Rosmarinus offficinalis) on lipid profiles and blood glucose in human diabetic patients (type-2). Afr. J. Biochem. Res. 2014; 8(8): 147-50.
[23] Louay Labban, Usama El-Sayed Mustafa and Yasser Mahmoud Ibrahim: The Effects of Rosemary (Rosmarinus officinalis) Leaves Powder on Glucose Level, Lipid Profile and Lipid Perodoxation. International Journal of Clinical Medicine 2014; 5: 297-304.
[24] Yousif Yahia Bilto, Nessrin Ghazi Alabdallat and Maher Salim: Antioxidant Properties of Twelve Selected Medicinal Plants Commonly Used in Jordan. British Journal of Pharmaceutical Research 2015; 6(2): 121-30.
[25] Bilto YY, Player M, Stuart J: Rheological action of oxpentifylline and structurally related xanthine derivatives on human erythrocytes. Clinical Hemorheology 1988; 8(2): 213-21.
[26] Srour, MA, Bilto, YY and Juma, M: Evaluation of different methods used to measure malonyldialdehyde in human erythrocytes. Clin. Hemorheol. Microcirc. 2000; 23:23-30.
[27] Reznick, A and Packer, L: Oxidative damage to proteins: Spectrophotometric method for carbonyl assay. Methods in Enzymology 1994; 233:357-63.
[28] Ellman, GL: Tissue Sulfhydryl (-SH) Groups. Archive of Biochemistry and Biophysiology 1951; 82:70-77.
[29] Yousif Yahia Bilto and Nessrin Ghazi Alabdallat: Ex vivo and In vivo Antioxidant Related Effects of Zingiber officinale Roscoe (Ginger) Extracts in Humans. European Journal of Medicinal Plants 2015; 7(2): 99-108.
[30] Arthur, JR and Boyne, R: Superoxide dismutase and glutathione peroxidase activities in neutrophilis from selenium deficient and copper deficient cattle. Life Sci. 1985; 36:1569-75.
[31] Zheng, W. and Wang, S.Y: Antioxidant Activity and Phenolic Compounds in Selected Herbs. Journal of Agricultural and Food Chemistry 2001; 49:5165-70.
[32] Zhang, X: Traditional Medicine and WHO World Health. The Magazine of World Health Organization 1996; 2:4-5.
[33] Moeko Noguchi-Shinohara, Kenjiro Ono, Tsuyoshi Hamaguchi, Kazuo Iwasa, Toshitada Nagai, Shoko Kobayashi, Hiroyuki Nakamura and Masahito Yamada: Pharmacokinetics, Safety and Tolerability of Melissa officinalis Extract which Contained Rosmarinic Acid in Healthy Individuals: A Randomized Controlled Trial. PLoS One 2015; 10(5): e0126422.
[34] Azab El Saied Azab, Fathy Ahmed Fetouh and Mohamed Omer Albasha: Nephro-Protective Effects of Curcumin, Rosemary and Propolis against Gentamicin Induced Toxicity in Guinea Pigs: Morphological and Biochemical Study. American Journal of Clinical and Experimental Medicine 2014; 2(2): 28-35.
[35] Atef M. Al-Attar and Nessreen A. Shawush: Physiological investigations on the effect of olive and rosemary leaves extracts in male rats exposed to thioacetamide. Saudi Journal of Biological Sciences 2014; 21:473-80.
[36] Mohsen Sahraei, Rasoul Pirmohammadi and Sina Payvastegan: The effect of rosemary (Rosmarinus officinalis L.) essential oil on digestibility, ruminal fermentation and blood metabolites of Ghezel sheep fed barley-based diets. Spanish Journal of Agricultural Research 2014; 12(2): 448-54.
[37] Umit Polat, Derya Yesilbag and Mustafa Eren: Serum Biochemical Profile of Broiler Chickens Fed Diets Containing Rosemary and Rosemary Volatile Oil. J. BIOL. ENVIRON. SCI. 2011; 5(13):23-30.
[38] Amera S. Abd El-Latif, Nahed S. Saleh, Tamer S. Allam and Emad W. Ghazy: The Effects of Rosemary (Rosemarinus afficinalis) and Garlic (Allium sativum) Essential Oils on Performance, Hematological, Biochemical and Immunological parameters of Broiler Chickens. British Journal of Poultry Sciences 2013; 2(2):16-24.
[39] Saber SA and Lamfon HA: Protective effect of rosemary (Rosmarinus Officinalis) leaves extracts on carbon tetrachloride-induced nephrotoxicity in albino rats. Life science journal 2012; 9(1): 779-85.
Cite This Article
  • APA Style

    Yousif Yahia Bilto, Nessrin Ghazi Alabdallat. (2015). In Vitro and in Vivo Antioxidant Related Effects of Rosemary (Rosmarinus Officinalis L.) Extracts in Humans. American Journal of Clinical and Experimental Medicine, 3(5), 213-221. https://doi.org/10.11648/j.ajcem.20150305.13

    Copy | Download

    ACS Style

    Yousif Yahia Bilto; Nessrin Ghazi Alabdallat. In Vitro and in Vivo Antioxidant Related Effects of Rosemary (Rosmarinus Officinalis L.) Extracts in Humans. Am. J. Clin. Exp. Med. 2015, 3(5), 213-221. doi: 10.11648/j.ajcem.20150305.13

    Copy | Download

    AMA Style

    Yousif Yahia Bilto, Nessrin Ghazi Alabdallat. In Vitro and in Vivo Antioxidant Related Effects of Rosemary (Rosmarinus Officinalis L.) Extracts in Humans. Am J Clin Exp Med. 2015;3(5):213-221. doi: 10.11648/j.ajcem.20150305.13

    Copy | Download

  • @article{10.11648/j.ajcem.20150305.13,
      author = {Yousif Yahia Bilto and Nessrin Ghazi Alabdallat},
      title = {In Vitro and in Vivo Antioxidant Related Effects of Rosemary (Rosmarinus Officinalis L.) Extracts in Humans},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {3},
      number = {5},
      pages = {213-221},
      doi = {10.11648/j.ajcem.20150305.13},
      url = {https://doi.org/10.11648/j.ajcem.20150305.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150305.13},
      abstract = {Rosemary (Rosmarinus officinalis L.) belongs to the family Lamiaceae (Labiatae) is a well-known aromatic plant used all around the world for different medicinal purposes. Aims: The present work aimed to investigate antioxidant related effects of rosemary in humans by in vitro and in vivo studies. Study design: Chemical antioxidant assays and venous blood from healthy volunteers were used to conduct in vitro experiments. For in vivo study, nine healthy volunteers, each received orally 250 ml of aqueous extract of rosemary daily for 5 days. Venous bloods were taken before and one hour after the first dose of aqueous extract (sample I and II respectively) and then one day after the last dose of day five (i.e. day 6, sample III). The first blood taken before the first dose (i.e. sample I), served as control for the next samples of II and III. Methodology: The following assays were performed: total antioxidant capacity (TAC), reducing power, scavenging activity of DPPH, scavenging activity of OH, iron chelating ability, erythrocyte reduced glutathione (GSH), malonyldialdehyde (MDA), protein carbonyl (PC), superoxide dismutase (SOD), percentage hemolysis, serum total antioxidant status (TAS) and serum selected biochemical tests. Results: Rosemary extract showed efficient total antioxidant capacity, reducing power, scavenging activity of DPPH, scavenging activity of OH and a reasonable iron chelating ability. Pre-incubation of erythrocytes in vitro with methanolic extract of rosemary then exposed to H₂O₂ decreased significantly MDA production (i.e. anti-lipid-peroxidant), PC production (i.e. anti-protein-oxidant) and oxidant hemolysis (i.e. anti-hemolytic) in a concentration dependent manner. Oral administration of aqueous extracts of rosemary to healthy volunteers, for 5 days, increased significantly serum TAS (18% increase), erythrocyte GSH (72% increase), erythrocyte superoxide dismutase (SOD) (21% increase) and decreased significantly erythrocyte MDA (17% decrease), with no effect on serum biochemical tests for kidney, liver, cardiac and pancreatic, compared to 0 time administration. Conclusion: Rosemary extracts have efficient in vitro and in vivo antioxidant related effects. As the present findings are obtained in healthy humans with no oxidative stress, this indicates that rosemary can improve the base line of the defense mechanisms against possible oxidative stress, with no adverse effects, thus decreasing susceptibility or preventing the progress of pathological conditions related to oxidative stress.},
     year = {2015}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - In Vitro and in Vivo Antioxidant Related Effects of Rosemary (Rosmarinus Officinalis L.) Extracts in Humans
    AU  - Yousif Yahia Bilto
    AU  - Nessrin Ghazi Alabdallat
    Y1  - 2015/09/16
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajcem.20150305.13
    DO  - 10.11648/j.ajcem.20150305.13
    T2  - American Journal of Clinical and Experimental Medicine
    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
    SP  - 213
    EP  - 221
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20150305.13
    AB  - Rosemary (Rosmarinus officinalis L.) belongs to the family Lamiaceae (Labiatae) is a well-known aromatic plant used all around the world for different medicinal purposes. Aims: The present work aimed to investigate antioxidant related effects of rosemary in humans by in vitro and in vivo studies. Study design: Chemical antioxidant assays and venous blood from healthy volunteers were used to conduct in vitro experiments. For in vivo study, nine healthy volunteers, each received orally 250 ml of aqueous extract of rosemary daily for 5 days. Venous bloods were taken before and one hour after the first dose of aqueous extract (sample I and II respectively) and then one day after the last dose of day five (i.e. day 6, sample III). The first blood taken before the first dose (i.e. sample I), served as control for the next samples of II and III. Methodology: The following assays were performed: total antioxidant capacity (TAC), reducing power, scavenging activity of DPPH, scavenging activity of OH, iron chelating ability, erythrocyte reduced glutathione (GSH), malonyldialdehyde (MDA), protein carbonyl (PC), superoxide dismutase (SOD), percentage hemolysis, serum total antioxidant status (TAS) and serum selected biochemical tests. Results: Rosemary extract showed efficient total antioxidant capacity, reducing power, scavenging activity of DPPH, scavenging activity of OH and a reasonable iron chelating ability. Pre-incubation of erythrocytes in vitro with methanolic extract of rosemary then exposed to H₂O₂ decreased significantly MDA production (i.e. anti-lipid-peroxidant), PC production (i.e. anti-protein-oxidant) and oxidant hemolysis (i.e. anti-hemolytic) in a concentration dependent manner. Oral administration of aqueous extracts of rosemary to healthy volunteers, for 5 days, increased significantly serum TAS (18% increase), erythrocyte GSH (72% increase), erythrocyte superoxide dismutase (SOD) (21% increase) and decreased significantly erythrocyte MDA (17% decrease), with no effect on serum biochemical tests for kidney, liver, cardiac and pancreatic, compared to 0 time administration. Conclusion: Rosemary extracts have efficient in vitro and in vivo antioxidant related effects. As the present findings are obtained in healthy humans with no oxidative stress, this indicates that rosemary can improve the base line of the defense mechanisms against possible oxidative stress, with no adverse effects, thus decreasing susceptibility or preventing the progress of pathological conditions related to oxidative stress.
    VL  - 3
    IS  - 5
    ER  - 

    Copy | Download

Author Information
  • Department of Biological Sciences, the University of Jordan, Amman, Jordan

  • College of Applied Medical Sciences, Majmaah University, Majmaah, Saudia Arabia

  • Sections