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Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model

Received: 25 March 2022     Accepted: 11 April 2022     Published: 22 April 2022
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Abstract

In this study, in vitro and in vivo experiments, it was studied soforaflavonozide (SFL) isolated from Alhagi canescens (Regel) B. Keller & Shap (Fabaceae (Leguminosae)), and narcissine isolated from Crocus sativus L. belonging to Iridaceae family. It was studied the effect of narcissine isolated from the plant on ATP-dependent potassium channel (mitoKATP-channel) activity in rat cardiac mitochondria. Animals of experimental group were divided into 4 groups: I control group (healthy), II experimental group (ischemia model), III experiment group (ischemia + narcissin), IV experimental group (ischemia + SFL). In rats with ischemia a 0.1 ml 0.1% solution of 100 mg/kg adrenaline was administered subcutaneously and peritoneally for 3 days in relative to body weight. The rats that underwent the ischemia model were given oral administration of 10 mg/kg of narcissine flavonoid to group III and 10 mg/kg of SFL flavonoid to group IV orally for 7 days. After that, in the experimental animals carried out electrocardiogram. Mitochondria from rat heart tissue were isolated by differential centrifugation. Cardiac mitoKATP-channel activity in the presence of ATP in an incubation medium was studied at concentrations of 10–50 μM of SFL and narcissine. Concentrations of 50 μM of SFL and narcissus and 30 μM of diazoxide were also found to have an activating effect on the mitochondrial channel of the heart. In the adrenaline-induced ischemia model, it was found that narcissine and SFL flavonoids restored the mitochondrial conduction permeability of the rat heart.

Published in American Journal of Biomedical and Life Sciences (Volume 10, Issue 2)
DOI 10.11648/j.ajbls.20221002.17
Page(s) 45-48
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Heart, Mitochondria, MitoKATP-channel, Adrenaline-induced Ischemia, Soforaflavonozide, Narcissine

References
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[2] Nishanbaev S. Z., Bobakulov Kh. M., Beshko N. Y., Shamyanov I. D. and Abdullaev N. D. (2017). Flavonoids of the above-ground part of Alaga sanessence flora of Uzbekistan // Chemistry of vegetable raw materials. V. 1. 77–83.
[3] Nishanbaev S. Z., Shamyanov I. D., Aripova S. F., Sagdullaev S. S. (2020). Metabolites of plants of the genus Alagi / Monograph. 204.
[4] Augustynek B., Wrzosek A., Koprowski P., Kiełbasa A., Bednarczyk P., Łukasiak A., Dołowy K. and Szewczyk A. (2016). What do we not know about mitochondrial potassium channels?, Postępy Biochemiiб, 62 (2): 189-197.
[5] Garlid K. D., Dos Santos P, Xie Z. J. et al. (2003). Mitochondrial potassium transport: the role of the mitochondrial ATP-sensitive K+ channel in cardiac function and cardioprotection. // Biochim Biophys Acta. 2003. 1606 (1-3). 1–21.
[6] Gayibov U., Gayibova, S. N., Pozilov M. K., Tuxtaeva F. S., Yusupova U. R., Djabbarova G. M.-K., Mamatova Z. A., Ergashev N. A. and Aripov T. F. (2021). Influence of quercetin and dihydroquercetin on some functional parameters of rat liver mitochondria, Journal of Microbiology, Biotechnology and Food Sciences, 11 (1): 1-7.
[7] Kholmuhamedov E. L., Jahangir A., Oberlin A., Komarov A., Colombini M. and Terzic A. (2004). Potassium channel openers are uncoupling protonophores: implication in cardioprotection, Febs Letters, 568 (1-3): 167-170.
[8] Kim J., Wei Y. and Sowers J. R. (2008). Role of mitochondrial dysfunction in insulin resistance, Circ Res, 102: 401–414.
[9] Mirzaolimov E. I., Pozilov M. K., Makhmudova Sh. R., Nishabaev S. Z. and Mirzaolimov M. M. (2021). Effect of sophoroflavonolonoside on the passive ion permeability of the cardiac mitochondria, «14th International Symposium on the chemistry of Natural compounds ABSTRACTS», Tashkent, Uzbekistan, 151.
[10] O’Rourke B. (2004). Evidence for mitochondrial K+ channels and their role in cardioprotection, Circ Res, 94: 420-432.
[11] Zhu H., Dong J., Zhu W., Ding H. and Zhou Z. (2003). ATP-dependent potassium channels involved in cardiac protection induced by intermittent hypoxia against ischemia-reperfusion injury, Life Sci, 73 (10): 1275-1287.
[12] Schneider W. C., Hageboom G. H. and Pallade G. E. (1948). Cytochemical studies of mammalian tissues; isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate material, J. Biol. Chem, 172 (2): 619–635.
[13] Peterson G. L. (1977). A simplification of the protein assay method of Lowry et al., which is more generally applicable, Analytical biochemistry, 83 (2): 346-356.
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    Mirzaolimov Elmurod Ismoilovich, Pozilov Mamurjon Komiljonovich, Abduqaxarov Abdugaffor Soyibjon O’g’li, Tursunov Davronbek Rahmonjon O’g’li, Afzalova Sayyora Abdulahadovna, et al. (2022). Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model. American Journal of Biomedical and Life Sciences, 10(2), 45-48. https://doi.org/10.11648/j.ajbls.20221002.17

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    Mirzaolimov Elmurod Ismoilovich; Pozilov Mamurjon Komiljonovich; Abduqaxarov Abdugaffor Soyibjon O’g’li; Tursunov Davronbek Rahmonjon O’g’li; Afzalova Sayyora Abdulahadovna, et al. Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model. Am. J. Biomed. Life Sci. 2022, 10(2), 45-48. doi: 10.11648/j.ajbls.20221002.17

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    AMA Style

    Mirzaolimov Elmurod Ismoilovich, Pozilov Mamurjon Komiljonovich, Abduqaxarov Abdugaffor Soyibjon O’g’li, Tursunov Davronbek Rahmonjon O’g’li, Afzalova Sayyora Abdulahadovna, et al. Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model. Am J Biomed Life Sci. 2022;10(2):45-48. doi: 10.11648/j.ajbls.20221002.17

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  • @article{10.11648/j.ajbls.20221002.17,
      author = {Mirzaolimov Elmurod Ismoilovich and Pozilov Mamurjon Komiljonovich and Abduqaxarov Abdugaffor Soyibjon O’g’li and Tursunov Davronbek Rahmonjon O’g’li and Afzalova Sayyora Abdulahadovna and Nishanbaev Sabir Zaripbaevich},
      title = {Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {10},
      number = {2},
      pages = {45-48},
      doi = {10.11648/j.ajbls.20221002.17},
      url = {https://doi.org/10.11648/j.ajbls.20221002.17},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20221002.17},
      abstract = {In this study, in vitro and in vivo experiments, it was studied soforaflavonozide (SFL) isolated from Alhagi canescens (Regel) B. Keller & Shap (Fabaceae (Leguminosae)), and narcissine isolated from Crocus sativus L. belonging to Iridaceae family. It was studied the effect of narcissine isolated from the plant on ATP-dependent potassium channel (mitoKATP-channel) activity in rat cardiac mitochondria. Animals of experimental group were divided into 4 groups: I control group (healthy), II experimental group (ischemia model), III experiment group (ischemia + narcissin), IV experimental group (ischemia + SFL). In rats with ischemia a 0.1 ml 0.1% solution of 100 mg/kg adrenaline was administered subcutaneously and peritoneally for 3 days in relative to body weight. The rats that underwent the ischemia model were given oral administration of 10 mg/kg of narcissine flavonoid to group III and 10 mg/kg of SFL flavonoid to group IV orally for 7 days. After that, in the experimental animals carried out electrocardiogram. Mitochondria from rat heart tissue were isolated by differential centrifugation. Cardiac mitoKATP-channel activity in the presence of ATP in an incubation medium was studied at concentrations of 10–50 μM of SFL and narcissine. Concentrations of 50 μM of SFL and narcissus and 30 μM of diazoxide were also found to have an activating effect on the mitochondrial channel of the heart. In the adrenaline-induced ischemia model, it was found that narcissine and SFL flavonoids restored the mitochondrial conduction permeability of the rat heart.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Effect of Soforaflavonozide and Narcissine Flavonoids on ATP-dependent Potassium Channels of Rat Cardiac Mitochondria in the Ischemia Model
    AU  - Mirzaolimov Elmurod Ismoilovich
    AU  - Pozilov Mamurjon Komiljonovich
    AU  - Abduqaxarov Abdugaffor Soyibjon O’g’li
    AU  - Tursunov Davronbek Rahmonjon O’g’li
    AU  - Afzalova Sayyora Abdulahadovna
    AU  - Nishanbaev Sabir Zaripbaevich
    Y1  - 2022/04/22
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ajbls.20221002.17
    DO  - 10.11648/j.ajbls.20221002.17
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 45
    EP  - 48
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20221002.17
    AB  - In this study, in vitro and in vivo experiments, it was studied soforaflavonozide (SFL) isolated from Alhagi canescens (Regel) B. Keller & Shap (Fabaceae (Leguminosae)), and narcissine isolated from Crocus sativus L. belonging to Iridaceae family. It was studied the effect of narcissine isolated from the plant on ATP-dependent potassium channel (mitoKATP-channel) activity in rat cardiac mitochondria. Animals of experimental group were divided into 4 groups: I control group (healthy), II experimental group (ischemia model), III experiment group (ischemia + narcissin), IV experimental group (ischemia + SFL). In rats with ischemia a 0.1 ml 0.1% solution of 100 mg/kg adrenaline was administered subcutaneously and peritoneally for 3 days in relative to body weight. The rats that underwent the ischemia model were given oral administration of 10 mg/kg of narcissine flavonoid to group III and 10 mg/kg of SFL flavonoid to group IV orally for 7 days. After that, in the experimental animals carried out electrocardiogram. Mitochondria from rat heart tissue were isolated by differential centrifugation. Cardiac mitoKATP-channel activity in the presence of ATP in an incubation medium was studied at concentrations of 10–50 μM of SFL and narcissine. Concentrations of 50 μM of SFL and narcissus and 30 μM of diazoxide were also found to have an activating effect on the mitochondrial channel of the heart. In the adrenaline-induced ischemia model, it was found that narcissine and SFL flavonoids restored the mitochondrial conduction permeability of the rat heart.
    VL  - 10
    IS  - 2
    ER  - 

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Author Information
  • Department of Fundamentals of Physiology, Namangan State University, Namangan, Uzbekistan

  • Department of Zoology and Anatomy, Tashkent State Pedagogical University Named After Nizami, Tashkent, Uzbekistan

  • Department of Fundamentals of Physiology, Namangan State University, Namangan, Uzbekistan

  • Department of Fundamentals of Physiology, Namangan State University, Namangan, Uzbekistan

  • Department of Zoology and Anatomy, Tashkent State Pedagogical University Named After Nizami, Tashkent, Uzbekistan

  • S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan

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