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Profile of Biochemical Markers and Viral Load in a Population of Blood Donors Infected with Hepatitis B and Naive Antiretroviral Treatment in Abidjan

Received: 13 November 2023     Accepted: 29 November 2023     Published: 8 December 2023
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Abstract

Hepatitis B is a viral infection of liver caused by a virus from the Hepadnavirus family. It’s a public health problem in sub-Saharan Africa and particularly in Côte d'Ivoire where it is poorly documented. This study's contribution to biological databases was significant. It aimed to establish the biochemical and viral load (VL) profile of hepatitis B in a population of blood donors infected with hepatitis B virus (HBV) and naïve to antiretroviral treatment in Abidjan. It was a descriptive cross-sectional study of voluntary blood donors of any sex, with a positive result for HBsAg and naive of any antiretroviral therapy. Venous blood samples of 4 ml were collected for biochemical marker determinations, quantitative antigen, and PCR VL. A questionnaire was also used to collect socio-demographic data from study participants The National Ethics Committee for Life Sciences and Health in Cote d'Ivoire granted its approval for the study (N/Réf: 196-22/MSHPCMU/CNESVS-km). 53 voluntary blood donors infected with HBV (HBsAg positive) were included in the study. 81.13% of participants were men. The average age of all participants was 35 ± 9 years, and the predominant age group was 30 to 40 (35.85%). Transaminase values were normal in 98.57% of the study population for ASAT and 96.23% for ALAT. Creatinine was normal in 90.57% of volunteers. Total proteidaemia, natremia, and kalemia were below normal in respectively 86.79%, 73.58% and 20.75% of this population. Quantitative HBsAg were high in 24.53% of the population. Viral load was elevated in 9.43% of patients. There was a significant association between increased VL in log and increased uremia. There was also a significant association between the increase in the amount of HBs antigen and the number of copies of the virus. The study noted a renal and hepatic balance without particularity. The ion balance was disrupted, and about a quarter of the study population had high values of quantitative HBs antigenemia. The VL was high in about one-tenth of the volunteers.

Published in American Journal of BioScience (Volume 11, Issue 6)
DOI 10.11648/j.ajbio.20231106.14
Page(s) 159-163
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Hepatitis B, Biochemistry Markers, Viral Load, Quantitative Antigen

References
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[2] Arikan Ayse, Murat Sayan, Tamer Sanlidag, Kaya Suer, Sinem Akcali, & Meryem Guvenir. 2019. Evaluation of the Pol/S Gene Overlapping Mutations in Chronic Hepatitis B Patients in Northern Cyprus. Polish Journal of Microbiology 68 (3): 317.
[3] Mokaya, Jolynne, Anna L. McNaughton, Martin J. Hadley, Apostolos Beloukas, Anna-Maria Geretti, Dominique Goedhals, & Philippa C. Matthews. 2018. A systematic review of hepatitis B virus (HBV) drug and vaccine escape mutations in Africa: A call for urgent action. PLoS Neglected Tropical Diseases 12 (8).
[4] Shimakawa Y, Njie R, Ndow G, Mendy M, et al. 2018. Development of a simple score based on HBe Ag and ALT for selecting patients for HBV treatment in Africa. Journal of Hepatology, 69, 776-784.
[5] Allah-Kouadio E, Yao BF, Mahassadi K, Assi C, & Lohouès-Kouacou MJ. 2016. Quelle stratégie de dépistage et de prise en charge des hépatites virales en entreprise. Revue Internationale des Sciences Médicales d'Abidjan, 1, 78 – 81.
[6] Attia K A, Eholié S, Messou E, Danel C, & Polneau S. 2012. Prevalence and virological proiles of hepatitis B infection in human immunodeiciency virus patients. World J Hepatol 27, 218-223.
[7] Pamatika CM, Mossoro-Kpindé CD, Diemer SCH, Kongo GNR, Kodia EL, Nguida H, & Longo JD. 2022. Prévalence de l’hépatite virale B chronique à Bangui et Bimbo en République Centrafricaine: cas des donneurs bénévoles réguliers non éligibles pour les dons de sang. Annales Africaines de Médecine, 15, 4810-4817.
[8] Doukou ES, Toni TA, N’din JL, Dechi RJ, Gogbe LO, N'guessan JF, et al. 2023. Molecular Characterization and Resistance Profile of the Hepatitis B Virus to Polymerase Inhibitors in Infected Treatment-Naïve Patients in Abidjan. American Journal of BioScience. 11(4): 92.
[9] Bonnefont-Rousselot D, Beaudeux JL, & Charpiot P. 2019. Explorations en biochimie médicale: interprétations et orientations diagnostiques. Edition Lavoisier, Médecine sciences, France, 395p.
[10] Mossoro-Kpindé CD, Gbangba-Ngai E, Mossoro-Kpindé HD, Camengo Police SM, Kobangué L, Selehina E, et al., 2016. Dépistage de l’antigène HBs chez les malades du VIH à Bangui. Revue Bio-Africa, 15, 39-43.
[11] Datta S, Soumya C and Vijay V. 2014. Recent advances in molecular diagnostics of hepatitis B virus. World Journal of Gastroenterology 20, 14615-25.
[12] N’din JLP. 2012. Détermination de la virémie de l’hépatite B chez des patients co-infectés ou non par le VIH au CIRBA. Mémoire de DEA de Biologie Humaine Tropicale, Abidjan, Université de Cocody, 47p.
[13] Salamanta D, Bassène ML, Gueye MN, Thioubou MA, & Daouda D. 2018. Hépatite virale B: aspects cliniques, paracliniques et évolutifs dans le service d’Hépato Gastroentérologie de l’Hôpital Aristide Le Dantec: à propos de 728 cas. Pan African Medical Journal, 31, 82- 89.
[14] Fanou D, Sehounou J, Vinasse A, Agniwo J, & Batcho J. 2019. Evaluation de l'état vaccinal contre l'hépatite B et portage de l'AgHBs chez les militaires béninois en missions en Côte d'Ivoire. Pan African Medical Journal, 32, 19-24.
[15] Nambei WS, Gamba EP, Gbangbangai E, Sombot-Ndicky S, Bogon A, & Senzongo O. 2014. Place de l’hépatite virale B seule ou associée au VIH parmi les causes d’affections hépatiques et rénales chez les adultes à Bangui, Centrafrique. Revue CAMES Santé, 2, 19-23.
[16] OMS 2018. Lignes directrices pour la prévention, les soins et le traitement en faveur des personnes atteintes d’une infection à hépatite B chronique. Document interne Genève, Licence: CC BY-NC-SA 3.0 IGO 143p.
[17] Sogoba D, Konaté I, Goita D, Dembélé J. P, et al. 2020. Les Troubles Électrolytiques dans un Groupe de Patients Hospitalisés pour Infection à VIH à Bamako. Health Sciences And Disease 21 (7).
[18] Chan H. L, Thompson A, Martinot-Peignoux M, Piratvisuth T, Cornberg M. 2011. Hepatitis B surface antigen quantification: Why and how to use it in 2011. A core group report. J Hepatol, 55: 1121-31.
[19] Séhonou J, Kpossou AR, Taofick OA, Comlan NM., Vignon RK, Vigan J. 2018. Hépatite virale B et insuffisance rénale: prévalence et facteurs associés au Centre National Hospitalier et Universitaire de Cotonou. Pan African Medical Journal, 31, 121-126.
Cite This Article
  • APA Style

    Samuel, D. E., Claude, L. E., Eugene, M. K., Philippe, N. J., Philippe, K. S., et al. (2023). Profile of Biochemical Markers and Viral Load in a Population of Blood Donors Infected with Hepatitis B and Naive Antiretroviral Treatment in Abidjan. American Journal of BioScience, 11(6), 159-163. https://doi.org/10.11648/j.ajbio.20231106.14

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    ACS Style

    Samuel, D. E.; Claude, L. E.; Eugene, M. K.; Philippe, N. J.; Philippe, K. S., et al. Profile of Biochemical Markers and Viral Load in a Population of Blood Donors Infected with Hepatitis B and Naive Antiretroviral Treatment in Abidjan. Am. J. BioScience 2023, 11(6), 159-163. doi: 10.11648/j.ajbio.20231106.14

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    AMA Style

    Samuel DE, Claude LE, Eugene MK, Philippe NJ, Philippe KS, et al. Profile of Biochemical Markers and Viral Load in a Population of Blood Donors Infected with Hepatitis B and Naive Antiretroviral Treatment in Abidjan. Am J BioScience. 2023;11(6):159-163. doi: 10.11648/j.ajbio.20231106.14

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  • @article{10.11648/j.ajbio.20231106.14,
      author = {Doukou Essien Samuel and Lohoues Esmel Claude and Messou Kouassi Eugene and N’din Jean-Louis Philippe and Kambou Sansan Philippe and Mamadou Sekongo and Tiahou Gnomblesson Georges},
      title = {Profile of Biochemical Markers and Viral Load in a Population of Blood Donors Infected with Hepatitis B and Naive Antiretroviral Treatment in Abidjan},
      journal = {American Journal of BioScience},
      volume = {11},
      number = {6},
      pages = {159-163},
      doi = {10.11648/j.ajbio.20231106.14},
      url = {https://doi.org/10.11648/j.ajbio.20231106.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20231106.14},
      abstract = {Hepatitis B is a viral infection of liver caused by a virus from the Hepadnavirus family. It’s a public health problem in sub-Saharan Africa and particularly in Côte d'Ivoire where it is poorly documented. This study's contribution to biological databases was significant. It aimed to establish the biochemical and viral load (VL) profile of hepatitis B in a population of blood donors infected with hepatitis B virus (HBV) and naïve to antiretroviral treatment in Abidjan. It was a descriptive cross-sectional study of voluntary blood donors of any sex, with a positive result for HBsAg and naive of any antiretroviral therapy. Venous blood samples of 4 ml were collected for biochemical marker determinations, quantitative antigen, and PCR VL. A questionnaire was also used to collect socio-demographic data from study participants The National Ethics Committee for Life Sciences and Health in Cote d'Ivoire granted its approval for the study (N/Réf: 196-22/MSHPCMU/CNESVS-km). 53 voluntary blood donors infected with HBV (HBsAg positive) were included in the study. 81.13% of participants were men. The average age of all participants was 35 ± 9 years, and the predominant age group was 30 to 40 (35.85%). Transaminase values were normal in 98.57% of the study population for ASAT and 96.23% for ALAT. Creatinine was normal in 90.57% of volunteers. Total proteidaemia, natremia, and kalemia were below normal in respectively 86.79%, 73.58% and 20.75% of this population. Quantitative HBsAg were high in 24.53% of the population. Viral load was elevated in 9.43% of patients. There was a significant association between increased VL in log and increased uremia. There was also a significant association between the increase in the amount of HBs antigen and the number of copies of the virus. The study noted a renal and hepatic balance without particularity. The ion balance was disrupted, and about a quarter of the study population had high values of quantitative HBs antigenemia. The VL was high in about one-tenth of the volunteers.
    },
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Profile of Biochemical Markers and Viral Load in a Population of Blood Donors Infected with Hepatitis B and Naive Antiretroviral Treatment in Abidjan
    AU  - Doukou Essien Samuel
    AU  - Lohoues Esmel Claude
    AU  - Messou Kouassi Eugene
    AU  - N’din Jean-Louis Philippe
    AU  - Kambou Sansan Philippe
    AU  - Mamadou Sekongo
    AU  - Tiahou Gnomblesson Georges
    Y1  - 2023/12/08
    PY  - 2023
    N1  - https://doi.org/10.11648/j.ajbio.20231106.14
    DO  - 10.11648/j.ajbio.20231106.14
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 159
    EP  - 163
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20231106.14
    AB  - Hepatitis B is a viral infection of liver caused by a virus from the Hepadnavirus family. It’s a public health problem in sub-Saharan Africa and particularly in Côte d'Ivoire where it is poorly documented. This study's contribution to biological databases was significant. It aimed to establish the biochemical and viral load (VL) profile of hepatitis B in a population of blood donors infected with hepatitis B virus (HBV) and naïve to antiretroviral treatment in Abidjan. It was a descriptive cross-sectional study of voluntary blood donors of any sex, with a positive result for HBsAg and naive of any antiretroviral therapy. Venous blood samples of 4 ml were collected for biochemical marker determinations, quantitative antigen, and PCR VL. A questionnaire was also used to collect socio-demographic data from study participants The National Ethics Committee for Life Sciences and Health in Cote d'Ivoire granted its approval for the study (N/Réf: 196-22/MSHPCMU/CNESVS-km). 53 voluntary blood donors infected with HBV (HBsAg positive) were included in the study. 81.13% of participants were men. The average age of all participants was 35 ± 9 years, and the predominant age group was 30 to 40 (35.85%). Transaminase values were normal in 98.57% of the study population for ASAT and 96.23% for ALAT. Creatinine was normal in 90.57% of volunteers. Total proteidaemia, natremia, and kalemia were below normal in respectively 86.79%, 73.58% and 20.75% of this population. Quantitative HBsAg were high in 24.53% of the population. Viral load was elevated in 9.43% of patients. There was a significant association between increased VL in log and increased uremia. There was also a significant association between the increase in the amount of HBs antigen and the number of copies of the virus. The study noted a renal and hepatic balance without particularity. The ion balance was disrupted, and about a quarter of the study population had high values of quantitative HBs antigenemia. The VL was high in about one-tenth of the volunteers.
    
    VL  - 11
    IS  - 6
    ER  - 

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Author Information
  • Care, Research and Training Center (CePReF), Abidjan, Côte d’Ivoire

  • Biochemistry Laboratory of Medical Sciences Department, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire

  • Care, Research and Training Center (CePReF), Abidjan, Côte d’Ivoire

  • Biochemistry Laboratory of Medical Sciences Department, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; Center Integrated of Research Bioclinical of Abidjan (CIRBA), Abidjan, Côte d’Ivoire

  • Biochemistry Laboratory of Medical Sciences Department, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire

  • National Blood Transfusion Centre of Abidjan (CNTS), Abidjan, Côte d’Ivoire

  • Biochemistry Laboratory of Medical Sciences Department, Alassane Ouattara University, Bouake, Côte d’Ivoire

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