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Randomized Clinical Trial to Analyze the Efficacy of an Eggshell Membrane Dietary Supplementation in the Concomitant Treatment of Osteoarthritis in Dogs

Osteoarthritis has become one of the most prevalent conditions that affect joints in dogs. Cartilage degradation and subsequent chronic inflammatory process affect synovial membranes, induce pain and decrease joint functionality. The aim of this research is to study the effects of supplementation with a nutraceutical based on internal egg shell membrane in dogs diagnosed with osteoarthritis in knee or hip joints. During a consumption period of 10 weeks, efficacy was evaluated in a placebo controlled clinical trial. Bioarth functional scale (BAS) was used to estimate joint functionality. Serum levels of C-telopeptides of type II collagen (CTx-II) were also collected as a biomarker of osteoarthritis. Functional limitation and joint mobility parameters showed an improvement by a decrease of BAS scores, although muscle atrophy showed no differences between any of the groups or periods considered in this study. Sterilization seems to have a higher influence in that parameter, indicating sex and condition as confounding variables. Antalgic postures significantly decreased in treatment group therefore indicating an improvement in the quality of life of subjects. Despite the fact that biomarker CTx-II has been used frequently in evaluation of cartilage degradation, no significant differences were observed in this study, although individual analysis revealed a greater reduction in a higher percentage of dogs from the treatment group. Osteoarthritis is a long-term disease, often frustrating for both veterinaries and owners. ESM could become a safe, effective alternative for chronic, long-term management of osteoarthritis in dogs.

Knee Pain, Hip Pain, Nutraceutical, Joint Functionality, Bioarth Assessment Scale

APA Style

Fernando Cánovas, David Planes-Muñoz, Miren Jaione Zurbano, Pablo Messia, María Isabel Vasallo. (2023). Randomized Clinical Trial to Analyze the Efficacy of an Eggshell Membrane Dietary Supplementation in the Concomitant Treatment of Osteoarthritis in Dogs. World Journal of Food Science and Technology, 7(3), 41-47. https://doi.org/10.11648/j.wjfst.20230703.11

ACS Style

Fernando Cánovas; David Planes-Muñoz; Miren Jaione Zurbano; Pablo Messia; María Isabel Vasallo. Randomized Clinical Trial to Analyze the Efficacy of an Eggshell Membrane Dietary Supplementation in the Concomitant Treatment of Osteoarthritis in Dogs. World J. Food Sci. Technol. 2023, 7(3), 41-47. doi: 10.11648/j.wjfst.20230703.11

AMA Style

Fernando Cánovas, David Planes-Muñoz, Miren Jaione Zurbano, Pablo Messia, María Isabel Vasallo. Randomized Clinical Trial to Analyze the Efficacy of an Eggshell Membrane Dietary Supplementation in the Concomitant Treatment of Osteoarthritis in Dogs. World J Food Sci Technol. 2023;7(3):41-47. doi: 10.11648/j.wjfst.20230703.11

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. Rychel J. K. (2010). Diagnosis and treatment of osteoarthritis. Topics in companion animal medicine, 25 (1), 20-25. https://doi.org/10.1053/j.tcam.2009.10.005
2. Taylor, C. L. (2004). Regulatory frameworks for functional foods and dietary supplements. Nutr. Rev, 62, 55–59.
3. Johnson, K. A., Lee, A. H., & Swanson, K. S. (2020). Nutrition and nutraceuticals in the changing management of osteoarthritis for dogs and cats. Journal of the American Veterinary Medical Association, 256 (12), 1335–1341. https://doi.org/10.2460/javma.256.12.1335
4. Barbeau-Grégoire, M.; Otis, C.; Cournoyer, A.; Moreau, M.; Lussier, B.; Troncy, E. A 2022 Systematic Review and Meta-Analysis of Enriched Therapeutic Diets and Nutraceuticals in Canine and Feline Osteoarthritis. Int. J. Mol. Sci., 23, 2022, 10384. https://doi.org/10.3390/ijms231810384
5. Anderson KL, Zulch H, O’Neill DG, Meeson RL and Collins LM (2020) Risk Factors for Canine Osteoarthritis and Its Predisposing Arthropathies: A Systematic Review. Front. Vet. Sci. 7, 220. https/doi.org/10.3389/fvets.2020.00220
6. Budsberg, S. C., & Bartges, J. W. (2006). Nutrition and osteoarthritis in dogs: does it help?. The Veterinary clinics of North America. Small animal practice, 36 (6), 1307–vii. https://doi.org/10.1016/j.cvsm.2006.08.007
7. Marshall, W., Bockstahler, B., Hulse, D., & Carmichael, S. (2009). A review of osteoarthritis and obesity: current understanding of the relationship and benefit of obesity treatment and prevention in the dog. Veterinary and comparative orthopaedics and traumatology: V. C. O. T, 22 (5), 339–345. https://doi.org/10.3415/VCOT-08-08-0069
8. Pye, C., Bruniges, N., Peffers, M. and Comerford, E. (2022), Advances in the pharmaceutical treatment options for canine osteoarthritis. J Small Anim Pract, 63: 721-738. https://doi.org/10.1111/jsap.13495
9. Schulze-Tanzil, G. (2021) Experimental therapeutics for the treatment of osteoarthritis. Journal of Experimental Pharmacology 13, 101-125.
10. Eckert, T.; Jährling-Butkus, M.; Louton, H.; Burg-Roderfeld, M.; Zhang, R.; Zhang, N.; Hesse, K.; Petridis, A. K.; Kožár, T.; Steinmeyer, J.; et al. (2021). Efficacy of Chondroprotective Food Supplements Based on Collagen Hydrolysate and Compounds Isolated from Marine Organisms. Mar. Drugs, 19, 542. https://doi.org/10.3390/md19100542
11. Yazaki, M., Ito, Y., Yamada, M., Goulas, S., Teramoto, S., Nakaya, M. A., Ohno, S., & Yamaguchi, K. (2017). Oral Ingestion of Collagen Hydrolysate Leads to the Transportation of Highly Concentrated Gly-Pro-Hyp and Its Hydrolyzed Form of Pro-Hyp into the Bloodstream and Skin. Journal of agricultural and food chemistry, 65 (11), 2315–2322. https://doi.org/10.1021/acs.jafc.6b05679
12. Bagchi, D., Misner, B., Bagchi, M., Kothari, S. C., Downs, B. W., Fafard, R. D., & Preuss, H. G. (2002). Effects of orally administered undenatured type II collagen against arthritic inflammatory diseases: a mechanistic exploration. International journal of clinical pharmacology research, 22 (3-4), 101–110.
13. Jabbari, M., Barati, M., Khodaei, M., Babashahi, M., Kalhori, A., Tahmassian, A. H., Mosharkesh, E., Arzhang, P., & Eini-Zinab, H. (2022). Is collagen supplementation friend or foe in rheumatoid arthritis and osteoarthritis? A comprehensive systematic review. International journal of rheumatic diseases, 25 (9), 973–981. https://doi.org/10.1111/1756-185X.14382
14. Aguirre, A.; Gil-Quintana, E.; Fenaux, M.; Sanchez, N.; Torre, C. (2018). The efficacy of Ovopet® in the treatment of hip dysplasia in dogs. J. Vet. Med. Anim. Health, 10, 198–207.
15. Beynen, A. C.; van Geene, H. W.; Grim, H. V.; Jacobs, P.; Van der Vlerk, T. (2010). Oral administration of gelatin hydrolysate reduces clinical signs of canine osteoarthritis in a double-blind, placebo-controlled trial. Am. J. Anim. Vet. Sci., 5, 102-106.
16. D’Altilio, M.; Peal, A.; Alvey, M.; Simms, C.; Curtsinger, A.; Gupta, R. C.; Canerdy, T. D.; Goad, J. T.; Bagchi, M.; Bagchi, D. (2007). Therapeutic Efficacy and Safety of Undenatured Type II Collagen Singly or in Combination with Glucosamine and Chondroitin in Arthritic Dogs. Toxicol. Mech. Methods, 17, 189-196.
17. Deparle, L. A.; Gupta, R. C.; Canerdy, T. D.; Goad, J. T.; D’Altilio, M.; Bagchi, M.; Bagchi, D. Efficacy and safety of glycosylated undenatured type-II collagen (UC-II) in therapy of arthritic dogs. J. Vet. Pharmacol. Ther. 2005, 28, 385-390.
18. Gupta, R. C.; Canerdy, T. D.; Lindley, J.; Konemann, M.; Minniear, J.; Carroll, B. A.; Hendrick, C.; Goad, J. T.; Rohde, K.; Doss, R.; et al. (2012). Comparative therapeutic efficacy and safety of type-II collagen (UC-II), glucosamine and chondroitin in arthritic dogs: Pain evaluation by ground force plate. J. Anim. Physiol. Anim. Nutr., 96, 770-777.
19. Muller, C.; Enomoto, M.; Buono, A.; Steiner, J. M.; Lascelles, B. D. X. (2019). Placebo-controlled pilot study of the effects of an eggshell membrane-based supplement on mobility and serum biomarkers in dogs with osteoarthritis. Vet. J., 253, 105379.
20. Peal, A.; D’Altilio, M.; Simms, C.; Alvey, M.; Gupta, R. C.; Goad, J. T.; Canerdy, T. D.; Bagchi, M.; Bagchi, D. (2007). Therapeutic efficacy and safety of undenatured type-II collagen (UC-II) alone or in combination with (-)-hydroxycitric acid and chromemate in arthritic dogs. J. Vet. Pharmacol. Ther., 30, 275–278.
21. Ruff, K. J.; Kopp, K. J.; Von Behrens, P.; Lux, M.; Mahn, M.; Back, M. (2016). Effectiveness of NEM (®) brand eggshell membrane in the treatment of suboptimal joint function in dogs: A multicenter, randomized, double-blind, placebo-controlled study. Vet. Med., 7, 113–121.
22. Stabile, M.; Samarelli, R.; Trerotoli, P.; Fracassi, L.; Lacitignola, L.; Crovace, A.; Staffieri, F. (2019). Evaluation of the Effects of Undenatured Type II Collagen (UC-II) as Compared to Robenacoxib on the Mobility Impairment Induced by Osteoarthritis in Dogs. Vet. Sci., 6, 72.
23. Brunello, E. and Masini, A. (2016) NEM Brand Eggshell Membrane Effective in the Treatment of Pain and Stiffness Associated with Osteoarthritis of the Knee in an Italian Study Population. International Journal of Clinical Medicine, 7, 169-175. DOI: 10.4236/ijcm.2016.72017.
24. Cánovas, F.; Abellán-Ruíz, M. S.; García-Muñoz, A. M.; Luque-Rubia, A. J.; Victoria-Montesinos, D.; Pérez-Piñero, S.; Sánchez-Macarro, M.; López-Román, F. J. (2022). Randomised Clinical Trial to Analyse the Efficacy of Eggshell Membrane to Improve Joint Functionality in Knee Osteoarthritis. Nutrients, 14, 2340. https://doi.org/10.3390/nu14112340
25. Danesch, U., Seybold, M., Rittinghausen, R., Treibel, W. and Bitterlich, N. (2014) NEM® Brand Eggshell Membrane Effective in the Treatment of Pain Associated with Knee and Hip Osteoarthritis: Results from a Six-Center, Open-Label German Clinical Study. Journal of Arthritis, 3, 136. DOI: 10.4172/2167-7921.1000136.
26. Ruff, K. J., DeVore, D. P., Leu, M. D. and Robinson, M. A. (2009) Eggshell Membrane: A Possible New Natural Therapeutic for Joint and Connective Tissue Disorders. Results from Two Open-Label Human Clinical Studies. Clinical Interventions in Aging, 4, 235-240. DOI: 10.2147/CIA.S5797.
27. Ruff, K. J., Winkler, A., Jackson, R. W., DeVore, D. P. and Ritz, B. W. (2009) Eggshell Membrane in the Treatment of Pain and Stiffness from Osteoarthritis of the Knee: A Randomized, Multicenter, Double-Blind, Placebo-Controlled Clinical Study. Clinical Rheumatology, 28, 907-914. DOI: 10.1007/s10067-009-1173-4.
28. Duclos, M. E., Roualdes, O., Cararo, R., Rousseau, J. C., Roger, T., & Hartmann, D. J. (2010). Significance of the serum CTX-II level in an osteoarthritis animal model: a 5-month longitudinal study. Osteoarthritis and cartilage, 18 (11), 1467–1476. https://doi.org/10.1016/j.joca.2010.07.007
29. Millis, D., Levine, D. (2009) Canine Rehabilitation and Physical Therapy. Saunders.
30. Vilar, J. M., Cuervo, B., Rubio, M., Sopena, J., Domínguez, J. M., Santana, A., & Carrillo, J. M. (2016). Effect of intraarticular inoculation of mesenchymal stem cells in dogs with hip osteoarthritis by means of objective force platform gait analysis: concordance with numeric subjective scoring scales. BMC veterinary research, 12 (1), 223. https://doi.org/10.1186/s12917-016-0852-z
31. R Development Core Team (2022). R: A language and environment for statistical computing Vienna, Austria.
32. Jiao, Q., Pruznak, A. M., Huber, D., Vary, T. C., & Lang, C. H. (2009). Castration differentially alters basal and leucine-stimulated tissue protein synthesis in skeletal muscle and adipose tissue. American journal of physiology. Endocrinology and metabolism, 297 (5), E1222–E1232. https://doi.org/10.1152/ajpendo.00473.2009