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Status and Significance of Citrus Greening (Huanglongbing) Disease in Ethiopia

The huaglongbing (HLB) is devastating citrus disease and cause total yield loss under favorable environmental conditions. The Candidatus Liberibacter species is the cause of citrus greening which is carried on Diaphorina citri during feeding of leaves. In Ethiopia citrus are produced in large commercial and small scale farmers. However, the status of yield reduction has no more attention were given. The bacteria species associated with HLB disease like Ca. Liberibacter asiaticus, Ca. L. africanus and Ca. L. americanus. The vector Diaphorina citri of Ca. Liberibacter asiaticus is heat tolerant and able to adapt temperature above 32°C compared with Ca. L. americanus’ and Ca. L. africanus. The field diagnosis of citrus greening is very difficult tasks and even confusing with symptoms of nutrient deficiency. From all the citrus, HLB is the most destructive and totally decline the production. The typical symptoms of greening disease like asymmetrical blotchy mottling pattern on central leaves, lopsided fruits, bitter tests and greening symptoms only observed in one side of the canopy. Therefore critical diagnosis is important task either the citrus tree has blotchy mottle or leaf yellowing due the deficiency of nutrient or HLB and to recommend disease management options. In present situation the citrus orchards infected with HLB is not be controlled however reducing the vector is possible using insecticides.

Blotchy Mottle, Candidatus Liberibacter, Citrus Greening, Diaphorina Citri, Huanglongbing

APA Style

Silas Chiko Sadamo. (2023). Status and Significance of Citrus Greening (Huanglongbing) Disease in Ethiopia. Plant, 11(3), 104-108.

ACS Style

Silas Chiko Sadamo. Status and Significance of Citrus Greening (Huanglongbing) Disease in Ethiopia. Plant. 2023, 11(3), 104-108. doi: 10.11648/j.plant.20231103.14

AMA Style

Silas Chiko Sadamo. Status and Significance of Citrus Greening (Huanglongbing) Disease in Ethiopia. Plant. 2023;11(3):104-108. doi: 10.11648/j.plant.20231103.14

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. Abate, T., 1988. The identity and bionomics of insect vectors of tristeza and greening diseases of Citrus in Ethiopia. International Journal of Pest Management, 34 (1), pp. 19-23.
2. Ajene, I. J., Khamis, F., Ballo, S., Pietersen, G., van Asch, B., Seid, N., Azerefegne, F., Ekesi, S. and Mohamed, S., 2020. Detection of Asian citrus psyllid (Hemiptera: Psyllidae) in Ethiopia: a new haplotype and its implication to the proliferation of Huanglongbing. Journal of Economic Entomology, 113 (4), pp. 1640-1647.
3. Ajene, I. J., Khamis, F., Mohamed, S., Rasowo, B., Ombura, F. L., Pietersen, G., van Asch, B. and Ekesi, S., 2019. First report of field population of Trioza erytreae carrying the huanglongbing-associated pathogen,‘Candidatus Liberibacter asiaticus’, in Ethiopia.
4. Atsbha, G. and Hintsa, M., 2018. Identification of insect and disease associated to citrus in Northern Ethiopia. African Journal of Microbiology Research, 12 (13), pp. 312-320.
5. Baldwin, E., Plotto, A., Bai, J., Manthey, J., Zhao, W., Raithore, S. and Irey, M., 2018. Effect of abscission zone formation on orange (Citrus sinensis) fruit/juice quality for trees affected by Huanglongbing (HLB). Journal of agricultural and food chemistry, 66 (11), pp. 2877-2890.
6. Bassanezi RB, Busato LA, Bergamin-Filho A, Amorim L, Gottwald TR. 2005. Preliminary spatial pattern analysis of Huanglongbing in Sao Paulo, Brazil. ˜ Proc. 16th Conf. Intern. Org. Citrus Virol., pp. 341–55. IOCV, Univ. Calif., Riverside, CA.
7. Bassanezi RB, Montesino LH, Amorim L, Gasparoto MCG, Bergamin-Filho A. 2008. Yield reduction caused by huanglongbing in different sweet orange cultivars in Sao Paulo, Brazil. ˜ Proc. Int. Res. Conf. Huanglongbing, pp. 270–273.
8. Bassanezi RB, Montesino LH, Stuchi ES. 2009. Effects of huanglongbing on fruit quality of sweet orange cultivars in Brazil. Europian Journal of Plant Pathology. 125: 565–572.
9. Bové JM 2006 Huanglongbing: a destructive, newly-emerging, century-old disease of citrus. Journa of Plant Pathology pp 7–37.
10. Bove, J. M., 2014. Huanglongbing or yellow shoot, a disease of Gondwanan origin: will it destroy citrus worldwide? Phytoparasitica 42 (5), 579–583.
11. Braswell, W. E., Park, J. W., Stansly, P. A., Kostyk, B. C., Louzada, E. S., da Graça, J. V., Kunta, M., 2020. Root samples provide early and improved detection of Candidatus Liberibacter asiaticus in Citrus. Sci. Rep. 10 (1), 1–11.
12. Catara, A., Azzaro, A., Mughal, S. M. and Khan, D. A., 1988, March. Virus, viroid and prokaryotic diseases of citrus in Pakistan. In Proceedings of 6th Conference of the International Organization of Citrus Virologist. Tel Aviv (pp. 957-962).
13. Catling HD, Atkinson PR. 1974. Spread of greening by Trioza erytreae (Del Guercio) in Swaziland. Proc. 6th Conf. Intern. Org. Citrus Virol., pp. 33–39. IOCV, Univ. Calif., Riverside, CA.
14. Da Graça, J. V. 2008. “Biology, history and world status of Huanglongbing,” in I Taller Internacional sobre Huanglongbing de los cítricos (Candidatus Liberibacter spp.) y el psílido asiático de los cítricos (Diaphorina citri) (Hermosillo), 1–7. Available online at:
15. Dala-Paula, B. M., Plotto, A., Bai, J., Manthey, J. A., Baldwin, E. A., Ferrarezi, R. S., Gloria, M. B. A., 2019. Effect of huanglongbing or greening disease on orange juice quality, a review. Front. Plant Sci. 9, 1976.
16. Dessie, B., Shimelash, D. and Gebeyehu, S., 2022. Detection of Huanglongbing, insect vectors and nutritional profile of citrus in Upper Awash, Ethiopia. Journal of Plant Pathology, 104 (1), pp. 17-31.
17. Garces-Giraldo, L. F., 2012. Cítricos: cultivo, poscosechae industrializacion. Available at:
18. Garnier, M., Jagoueix-Eveillard, S., Cronje, P. R., Le Roux, H. F. and Bove, J. M., 2000. Genomic characterization of a liberibacter present in an ornamental rutaceous tree, Calodendrum capense, in the Western Cape Province of South Africa. Proposal of'Candidatus Liberibacter africanus subsp. capensis'. International Journal of Systematic and Evolutionary Microbiology, 50 (6), pp. 2119-2125.
19. Gottwald, T. R., 2010. Current epidemiological understanding of citrus huanglongbing. Annu. Rev. Phytopathol. 48, 119–139.
20. Hailu, T. and Wakgari, M., 2019. Distribution and Damage of African Citrus Psyllids (Trioza erytreae) in Casimiroa edulis Producing Areas of the Eastern Zone of Ethiopia. International Journal of Environment, Agriculture and Biotechnology, 4 (3).
21. Hall, D. G. and Hentz, M. G., 2010. Sticky trap and stem–tap sampling protocols for the Asian citrus psyllid (Hemiptera: Psyllidae). Journal of Economic Entomology, 103 (2), pp. 541-549.
22. Hall, D. G., Richardson, M. L., Ammar, E. D., Halbert, S. E., 2013. Asian citrus psyllid, Diaphorina citri, vector of citrus huanglongbing disease. Entomol. Exp. Appl. 146, 207–223.
23. Inoue, H., Yamashita-Muraki, S., Fujiwara, K., Honda, K., Ono, H., Nonaka, T., Kato, Y., Matsuyama, T., Sugano, S., Suzuki, M. and Masaoka, Y., 2020. Fe2+ ions alleviate the symptom of citrus greening disease. International journal of molecular sciences, 21 (11), p. 4033.
24. Jagoueix, S., Bove, J. M. and Garnier, M., 1994. The phloem-limited bacterium of greening disease of citrus is a member of the α subdivision of the Proteobacteria. International Journal of Systematic and Evolutionary Microbiology, 44 (3), pp. 379-386.
25. León, G. and Kondo, T., 2018. Insectos y ácaros de los cítricos: compendio ilustrado de especies dañinas y benéficas, con técnicas para el manejo integrado de plagas.
26. Lin, K. H., 1956. Observations on yellow shoot of Citrus. Etiological studies of yellow shoot of Citrus. Acta Phytopathologica Sinica, 2 (1).
27. Martinelli, F. and Dandekar, A. M., 2017. Genetic mechanisms of the devious intruder Candidatus Liberibacter in citrus. Frontiers in plant science, 8, p. 904.
28. Monzó, C., Arevalo, H. A., Jones, M. M., Vanaclocha, P., Croxton, S. D., Qureshi, J. A. and Stansly, P. A., 2015. Sampling methods for detection and monitoring of the Asian citrus psyllid (Hemiptera: Psyllidae). Environmental Entomology, 44 (3), pp. 780-788.
29. Rasowo, B. A., 2019. Assessment of incidence, severity and distribution patterns of citrus greening in Kenya and Tanzania; the role of African Citrus Triozid endosymbionts in disease epidemiology (Doctoral dissertation, Universität Bonn).
30. Rwomushana, I., Khamis, F. M., Grout, T. G., Mohamed, S. A., Sétamou, M., Borgemeister, C., Heya, H. M., Tanga, C. M., Nderitu, P. W., Seguni, Z. S. and Materu, C. L., 2017. Detection of Diaphorina citri Kuwayama (Hemiptera: Liviidae) in Kenya and potential implication for the spread of Huanglongbing disease in East Africa. Biological Invasions, 19 (10), pp. 2777-2787.
31. Saponari, M., De Bac, G., Breithaupt, J., Loconsole, G., Yokomi, R. K. and Catalano, L., 2010. First report of ‘Candidatus Liberibacter asiaticus’ associated with Huanglongbing in sweet orange in Ethiopia. Plant disease, 94 (4), pp. 482-482.
32. Schwarz, R. E., Knorr, L. C. and Prommintara, M., 1973. Presence of citrus greening and its psylla vector in Thailand. FAO Plant Prot. Bull, 21, pp. 132-138.
33. Sétamou, M., Flores, D., Victor French, J. and Hall, D. G., 2008. Dispersion patterns and sampling plans for Diaphorina citri (Hemiptera: Psyllidae) in citrus. Journal of Economic Entomology, 101 (4), pp. 1478-1487.
34. Sétamou, M., Sanchez, A., Saldaña, RR., Patt, JM., Summy, R., 2014. Visual responses of adult Asian citrus psyllid (Hemiptera: Liviidae) to colored sticky traps on citrus trees. Journal of Insect Behaviors 27: 540–553.
35. Shimwela, M. M., Narouei-Khandan, H. A., Halbert, S. E., Keremane, M. L., Minsavage, G. V., Timilsina, S., Massawe, D. P., Jones, J. B. and van Bruggen, A. H., 2016. First occurrence of Diaphorina citri in East Africa, characterization of the Ca. Liberibacter species causing huanglongbing (HLB) in Tanzania, and potential further spread of D. citri and HLB in Africa and Europe. European Journal of Plant Pathology, 146 (2), pp. 349-368.
36. Stansly, P. A., Qureshi, J. and Arevalo, A., 2009. Why, when and how to monitor and manage Asian citrus psyllid. Citrus Ind, 90, pp. 23-24.
37. Tolba, I. H., Soliman, M. A., 2015. Citrus Huanglongbing (greening disease) in Egypt: symptoms documentation and pathogen detection. American-Eurasian Journal of Agriculture of Environmental Sciences 15, 2045–2058.
38. Vashisth, T., Kadyampakeni, D., 2020. Diagnosis and management of nutrient constraints in citrus. In: Srivastava, A. K., Hu, C. (Eds.), Fruit Crops: Diagnosis and Management of Nutrient Constraints. Elsevier, The Netherlands, pp. 723–737.
39. Wang, N., 2020. A perspective of citrus Huanglongbing in the context of the Mediterranean Basin. Journal of Plant Pathology, 102 (3), pp. 635-640.
40. Zhang, M. Q., Guo, Y., Powell, C. A., Doud, M. S., Yang, C. Y., Zhou, H. and Duan, Y. P., 2016. Zinc treatment increases the titre of ‘Candidatus Liberibacter asiaticus’ in huanglongbing-affected citrus plants while affecting the bacterial microbiomes. Journal of Applied Microbiology, 120 (6), pp. 1616-1628.
41. Zheng, Y., Kumar, N., Gonzalez, P. and Etxeberria, E., 2018. Strigolactones restore vegetative and reproductive developments in Huanglongbing (HLB) affected, greenhouse-grown citrus trees by modulating carbohydrate distribution. Scientia Horticulturae, 237, pp. 89-95.