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In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates

Most available antimicrobials are now ineffective and the whole world healthcare system is currently under threat of antimicrobial resistant infections. Consequently, study of plant bioactive compounds with potentials infectious diseases therapeutic values is of significant risen interest with biologically synthesized plant extracts derived silver nanoparticles on the greater focus. This research was aimed at determining the in vitro antimicrobial activity of silver nanoparticles synthesized from extracts of Terminalia avicennioides on antibiotic resistant Pseudomonas aeruginosa isolates from wounds. Standard phenotypic and genotypic techniques were used for the Isolation and identification of Pseudomonas aeruginosa isolates. Selected antibiotics, Terminalia avicennioides extracts and the extracts derived silver nanoparticles antimicrobial activities on the antibiotic resistant Pseudomonas aeruginosa were determined using standard tests methods. Findings showed the isolates to be resistant to 18.18% - 100% of the antibiotics used, but 100% sensitive to imipenem. Analysis of the plant extracts for bioactive compounds showed the presence of tannins, alkaloids, flavonoids, cardiac glycosides, phenols, saponins and terpenoids. Antimicrobial profile of Terminalia avicennioides extracts on the antibiotic resistant Pseudomonas aeruginosa isolates showed zones of growth inhibition ranged from 10.04±9.39 – 18.08±10.62 mm with no significant difference (P > 0.05), minimum inhibitory concentration ranged from 60.000+65.8281 -40.000 + 21.0821 mg/ml with no significant difference (p < 0.05), and minimum bactericidal concentration ranged from 100.00 ± 89.4427 – 63.6364 ± 50.4525 mg/ml with no significant difference (p > 0.05). The antimicrobial activity of the biologically synthesized silver nanoparticles on the antibiotic resistant Pseudomonas aeruginosa showed zone of growth inhibition ranged from 28.00 ± 13.51 – 53.00 ± 76.97 mm with no significant difference (p > 0.05). Terminalia avicennioides extracts and silver nanoparticles antimicrobial activity showed significant difference (p > 0.05). In comparison, the silver nanoparticles zones of growth inhibition was larger (28.39 ± 2.98 mm) than that of the extracts (16.83 ± 12.70 mm). This inferred that the synthesized silver nanoparticles possess potential of being used as a good chemotherapeutic agent for wound infections.

Pseudomonas aeruginosa, Antibiotic Resistant, Nanoparticles, Wound, Antimicrobial, Terminalia avicennioides

APA Style

Danjuma Lawal, Bobai Mathew, Sani Muhammad Nura. (2022). In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates. Journal of Biomaterials, 6(1), 5-19.

ACS Style

Danjuma Lawal; Bobai Mathew; Sani Muhammad Nura. In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates. J. Biomater. 2022, 6(1), 5-19. doi: 10.11648/j.jb.20220601.12

AMA Style

Danjuma Lawal, Bobai Mathew, Sani Muhammad Nura. In vitro Antimicrobial Evaluation of Biologically Synthesised Silver Nanoparticles from Terminalia avicennioides Extracts on Antibiotic Resistant Pseudomonas aeruginosa Isolates. J Biomater. 2022;6(1):5-19. doi: 10.11648/j.jb.20220601.12

Copyright © 2022 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. F. Shigeki, S. M. Kathryn, L. Y. Victor, Victor (2017). Pseudomonas aeruginosa: Antimicrobe: Infectious disease antimicrobial agent. on 15/10/2017
2. Who, 2014 World Health Organization, (WHO) (2014). Antimicrobial Resistance: Global Report on Surveillance. Available from:
3. M. Cowan Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12: 564–82.
4. Adebayo, J. O. and Krettli, A. U. Potential antimalarial from Nigerian plants: A review. Journal of Ethnopharmacology 2011; 133: 289–302.
5. S. Pirtarighat, M. Ghannadnia, and S. Baghshahi Green synthesis of silver nanoparticle using the plant extract of Salvia Spinoza grown in vitro and their antibacterial activity assessment. International Journal of Nanostructures in Chemistry 2019; 9: 1: 1-9.
6. F. P. Reuben and J. B. Paulo Traditional Therapies for Skin Wound Healing. Adv Wound Care (New Rochelle) 2016; 5 (5): 208–229. doi: 10.1089/wound.2013.0506PMCID: PMC4827280.
7. E. Velez, G. Campillo, G. Morales, C. Hiricape, J. Osoria and O. Arnache Silver nanoparticles obtained by aqueous or ethanolic Aloe vera extracts: An assessment of the antibacterial activity and Mercury removal capability. Journal of Nanomaterials; 2018.
8. S. J. Vallis and B. J. Nacente Hand book of Microbiological culture media, 9edition, Scherlau Chemie S. A., 2006; Pp 68.
9. M. Cheesbrough District Laboratory practice in tropical countries, part 2, low price edition, Cambridge university press 2010; 63-70, 91-105, 137-142, 178-186, 194-197
10. K. R. Aneja Experiment in Microbiology plant pathology biotechnology, 4th edition, new age international (p) Ltd, new Delhi new York. age 2007; Pp390.
11. J. O. Ochai and A. Kolhatkar Medical Laboratory Science and practice, Tata McGrew Hill publishing limited new Delhi, New York 2008; 535: 539, 632-635.
12. M. Bobai, L. Danjuma, N. M. Sani (2022). In vitro antibacterial activity of biologically synthesised silver nanoparticles using Terminalia avicennioides extracts against multidrug resistant Staphylococcus aureus strains. The journal of photo pharmacology, 11 (2): 64-74. doi: 10.31254/phyto.2022.11203.
13. D. R. Arora, and B. Arora A text book of Microbiology; 3rd edition, CBS Publisher, New Delhi 2011. Pp 75-80, 213 and 418.
14. CLSI. Perfomance standard for antimicrobial susceptibility testing; thirty edition 2020.
15. G. E. Trease, and W. C. Evans Pharmacognosy. 15th edition, London: Saunders publishers 2002; 42-44: 221-229: 246-249: 304-306: 331-332: 391-393.
16. J. B. Harbone Phytochemical methods. London Chapman and Hall Ltd 1996; 52-105.
17. E. A. Sofowara Research on medicinal plants and traditional medicine in Africa. Journal of alternative and complementary Medici medicine 1996; 2 (3): 365-372.
18. Association of Official Analytical Chemists (AOAC). Official Methods of Analysis of the Association of Official Analytical Chemists. 14th edn; Washington. D. C 1984.
19. C. C. Chang,, M. H. Yang,, H. M. Wen, J. C. Chern, Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. Journal of Food and Drug Analysis 2002; 10: 178-182.
20. H. O. Edeoga, D. E. Okwu, B. O. Mbaebie, Phytochemical constituent of some Nigerian medicinal plants. African Journal of Biotechnology 2005; 4 (7): 685-688.
21. O. I. Oloyed, Chemical Profile of Carical papaga. Parkistan Journal of Nutrition 2005; 4: 379-381.
22. P. Balashanmugam, and P. T. Kalaichelvan, Bogenic synthesis of silver nanoparticles from DodonaeA viscosa and its effective Antibacterial activity. Journal of scientific transaction, environment and technology 2014; 13 (2): 67-71.
23. F. A. Henry,, Henry. Kis and Andy Dilli. Synikes's of silver mano particles Using Aqueous attracts of medical plants (Impatiens balsamina and Lantana Camera) fresh leaves and Analysis of Antimicrobial Actuality. International Journal of Microbiology 2019.
24. V. C. Suresh, C. B. G. Subash, A. Periasamy, F. Neeraj, F. Shivkanya, M. Proveena, K. Kishonthani, V. R. Lebaka, R. Gobinath, S. Subramaniam, and S. Sumilka, Characterization and Antibacterial Response of Filer Nanoparticles Biosynthesized using an ethanolic extract of cocaine Indica leaves, Crystals 2021. 97, 0020097.
25. R. Prasanna, R. Balasubramanian, R. Kunal, V. Siddarthan, K. Amrita, S. Priyanka, M. Dilip, S. Yashbir, and K. Sandhya, Microbial Inoculants with Multifaceted Traits Suppress Rhizoctonia Populations and Promote Plant Growth in Cotton. J Phytopathol 2016; 164: 1030–1042.
26. H. Takase, H. Nitanai, K. Hoshino, T. Otani Impact of siderophore production on Pseudomonas aeruginosa infections in immunosuppressed mice. Infect Immun 2000; 68 (4): 1834-9.
27. I. L. Lamont, P. A. Beare, U. Ochsner, A. I. Vasil, M. L. Vasil Siderophore-mediated signaling regulates virulence factor production in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 2002; 99 (10): 7072-7.
28. A. H. A. Walthiq, and S. A. A. Mohammed, Molecular detection f or nosocomial Pseudomonas aeruginosa and its Relationship whit multiding resistance, Isolated from Hospitals government. Medico-Legal Update 2020; 20 (1): 633-6335.
29. S. F. Van Vuuren Antimicrobial activity of South African medicinal plants. Journal of Ethnopharmacology 2008; 119: 462–72.
30. L. M. Prescott, J. P. Harley, and A. D. Klein Microbiology; 7th edition, McGraw-Hill, New York 2008; pp 852-853: 53-54: 446-455: 832-838.
31. M. L. Emma, and K. Warren Background paper 6.1 antimicrobial resistance, priority medicines for Europe and the World, a public health approach to inovation. Boston University 2013.
32. A. O. Sara, C. Ariadnna, E. R. Gerardo, C. D Vicenta., E. Gerardo, A. Jose, H. Rigoberto, A. R Castro., and X. Juan Phenotypic characterization of multidrug-resistant Pseudomonas aeruginosa strains isolated from pediatric patients associated to biofilm formation. Microbiological Research 2015; 172: 68-78.
33. E. Mohammad, B. Maryam, S. Mhboubeh, A. Nafiseh, B. Reza, B. L. Willem, and J. Fereshteh, Evaluation of Mannosidase and Trypsin Enzymes Effects on Biofilm Production of Pseudomonas aeruginosa Isolated from Burn Wound Infections. PLoS ONE 2016; 11 (10): e0164622. doi: 10.1371/journal.pone.0164622.
34. G. M. Eliopoulos, S. E. Cosgrove, Y. Carmeli The impact of antibacterial resistance on health and economic outcomes. Clinical Infectious Diseases 2003; 36: 1433-1437.
35. D. N. Friedman, E. Temkin, Y. Carmeli The negative impact of antibiotic resistance. Clinical Microbiology and Infection 2016; 22 (5): 416-422;
36. R. T. Sadikot, T. S. Blackwell, J. W Christman., A. S. Prince Pathogen-host interactions in Pseudomonas aeruginosa pneumonia. American Journal of Respiratory and Critical CareMedicine 2005; 171 (11): 1209-23.
37. E. O. Odebunmi, O. O. Oluwaniyi, G. V. Awolola, and O. O. Adediji, Proximate and nutritional composition of kolanut (colantrida), Bitter Cola (Garcinia kola) and Alligator pepper (Aframomumn eclequeta), Polish African Journal of Biotechnology 2009; 8 (2): 308-310.
38. A. f Alaje. lim/ J/ Y/ I. Yoon. and C. J. Hovde A brief review of Escherichia coli 0157.H7 and its plasmid 0157. Journal of microbiology and biotechnology 2014; 20 (1): 5-14.
39. S. Irshad, M. Butt., and H. Younis. In vitro antibacterial activity of two medicinal plants: neem (Azadirachta indica) and peppermint. International Research Journal of Pharmaceuticals 2011; 01 (01): 9-14.
40. B. Radhika, N. Murthy, and D. Nirmala Preliminary phytochemical analysis and antibacterial activity against clinical pathogens of medically important Orchid Cymbidium aloifolium (L) SW. International Journal of pharmaceutical sciences and Research 2013; 4 (10): 3925-3931.
41. G. M. Cragg, D. J. Newman, Biodiversity: A continuing source of novel drug leads. Pure and Applied Chemistry, 2005; 77 (1): 7 – 24.
42. M. Abdullahi and A. K. Yusuf Antibacterial activity of methanolic extracts of Terminalia avicennioides against fish pathogenic bacteria. American Journal of Research Communication 2014; 2 (4):
43. P. S. Pavithra, V. S. Janani, K. H. Charumathi, R. Indumatjy, S. Potala, R. S. Verma Antibacterial activity of plants used in Indian herbal medicine. International journal of green pharmacy 2010; 4: 22-8.
44. D-L. Keshebo, and M. K. Choudhurg, Phytochemical in investigation of Securidoca longipeduncilara (polygalaceae) and Structure elucidation of benzyl 2-hydroxy-5-11/21/4024benzoase. International Journal of Current microbial and applied Science 2015; 4 (1): 490-65.
45. S. O. Onaja, M. I. M Ezeja Y. N. Omeh and B. C. Onwukwen Antioxidant, anti-inflamatory and antinoceptive activities of methanolic extract of Justcia secenda Vahl leaf. Alexander Journal of Medicine 2016; 14 (6): 56-63.
46. S. S. Ali, A. Ayuba, S. N. Ali, S. Begum, B. S. Siddiqui, M. Mahmou, and K. L. Khan, Antibacterial activity of methanol extracts from some Selected mechanical plants. FULAST Journal of Biological Sciences 2017; 7 (1): 123-125.
47. M. S. Udgire, and G. R. Pathade, Evaluation of antimicrobial activities and phytochemical constituents of extracts of Valeriana wallichii. Asian Journal of Plant Science and Research 2013; 3 (5): 55-59.
48. B. Anegbeh, and A. O. Sofomora, Qualitative phytochemical screening and in vitro antimicrobial effect methanol steam bark of Ficus thonningii. Journal Complementary and Alternative machine 2006; 3: 269-295.
49. C. Gebrechelema, B. Tepe, D. Deferera, M. Sokmen, M. Polisiou, and A. Sokmen. In vitro and antimicrobial and antioxidant activities of the Essential oils and venous Extracts of Thymus. Journal of Agriculture and food 2013; 52: 1132-1137.
50. F. Shadidi Antioxidants in food and food antioxidants. Food Nahrung 2000; 44: 158-163.
51. A. Mann, A. Y. Yahaya, A. Banso, and F. John Phytochemical and Antimicrobial activity of Terminalia avicennioides extracts against some bacteria pathogens associated with patients suffering from complicated respiratory tract diseases. Journal of Medicinal Plant Research 2011; 2 (5): 094-097.
52. E. I. Cock. The medicinal properties and phytochemistry of plants of the genus Terminalia (Combretaceae). Inflammopharmacology 2015; 23 (5): 203–229.
53. A. J. Qwidwai, R. Kumar, and A. Dikshit, Green Synthesis of Silver Nanoparticles by seed of phoenix syvestris L, and their role in the management of cometics embarrassment, Green Chemistry letters and Review 2018; 11 (2): 176-188, doi: 10.1080 /17518253, 2018, 1445301.
54. S. S. Khwaja, H. Azamal, and A. K. P. Rifaqat, A reviews on Biosynthesis of silver nanoparticles and the biocide properties. Journal of Nano biotechnology 2018; 16: 14.
55. M. Skandalis, A. Dimopoulou, A. Georgorgopoulou, N. Gallious, D. Papadopoulas, D. Tsipas, I. Theologidis, N. Michailidis, and M. Chatzinikolaidoy, The effect of filler nanoparticles and the size, produced using plant extract from Arbutus unedo on their antibacterial efficacy. Nanomaterials 2017; 7 (7): 178.
56. J. Marcinkiewicz, R. Biedron, A. Bialacka, A. Kasprowicz, M. Mak, and M. Targosz. Susceptibility of propionic bacterium acnes and Staphylococcus epidermichs to Killing by MPO - Halide system. Product. Implication for Taurinebromamine as a new Candidate for topical therapy in treating Acne vulgaris. Arch Immunol. Ther exp 2006; 54: 61-68.
57. L. H Wang, J. Tian, and X. Sun, Monodisperse, micrometer-scale highly crystalline. Nanoparticles Ag dendrites, rapid, large-scale wet- chemical synthesis and their application as SERS substrates SACS Applied mattes Interfaces 2010; 21: 2987-2991.