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Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice

Received: 9 May 2018     Accepted: 24 May 2018     Published: 10 December 2019
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Abstract

Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.

Published in World Journal of Public Health (Volume 4, Issue 4)
DOI 10.11648/j.wjph.20190404.14
Page(s) 96-101
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Achyranthes Aspera, Hematological Parameter, Albino Mice

References
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Cite This Article
  • APA Style

    Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. (2019). Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World Journal of Public Health, 4(4), 96-101. https://doi.org/10.11648/j.wjph.20190404.14

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    ACS Style

    Emmanuel Asuquo Etim; Yusuf Abdulhakeem Adebayo; Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J. Public Health 2019, 4(4), 96-101. doi: 10.11648/j.wjph.20190404.14

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    AMA Style

    Emmanuel Asuquo Etim, Yusuf Abdulhakeem Adebayo, Obeagu Emmanuel Ifeanyi. Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice. World J Public Health. 2019;4(4):96-101. doi: 10.11648/j.wjph.20190404.14

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  • @article{10.11648/j.wjph.20190404.14,
      author = {Emmanuel Asuquo Etim and Yusuf Abdulhakeem Adebayo and Obeagu Emmanuel Ifeanyi},
      title = {Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice},
      journal = {World Journal of Public Health},
      volume = {4},
      number = {4},
      pages = {96-101},
      doi = {10.11648/j.wjph.20190404.14},
      url = {https://doi.org/10.11648/j.wjph.20190404.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjph.20190404.14},
      abstract = {Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Effect of Achyranthes Aspera Leaf Extract on Hematological Parameters of Swiss Albino Mice
    AU  - Emmanuel Asuquo Etim
    AU  - Yusuf Abdulhakeem Adebayo
    AU  - Obeagu Emmanuel Ifeanyi
    Y1  - 2019/12/10
    PY  - 2019
    N1  - https://doi.org/10.11648/j.wjph.20190404.14
    DO  - 10.11648/j.wjph.20190404.14
    T2  - World Journal of Public Health
    JF  - World Journal of Public Health
    JO  - World Journal of Public Health
    SP  - 96
    EP  - 101
    PB  - Science Publishing Group
    SN  - 2637-6059
    UR  - https://doi.org/10.11648/j.wjph.20190404.14
    AB  - Background/Objective: Achyranthes aspera is an erect flowering medicinal herb use in folkloric medicine. This study aims to examine the effect of methanolic leaf extract of Achyranthes aspera on hematological parameters of Swiss albino mice. Materials and Methods: 70 adult mice of both sexes with mean weight of 22 ± 5g were used for this study. Achyranthes aspera leaves was pulverized to coarse powder from where methanolic extract was obtained. 0.2ml of 50mg/kg, 100mg/kg, and 200mg/kg respectively of leaf extract was administered to mice for four days. After administration, 1ml of blood was collected from mice through cardiac puncture into EDTA bottle for full blood count estimation using Abacus-80 hematological analyzer. Results: The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract resulted in increased lymphocyte count from 29.4±7.35% to 70.4±0.25%, 70.5±0.219% and 81.3±0.282% respectively at P>0.05 while neutrophil value was reduced from 55.2±11.05 x 109/l to 14.0±0.216x 109/l, 11.2±0.109x109/l and 12.3±0.148 x109/l. The administration of 50mg/kg, 100mg/kg and 200mg/kg doses of the extract increases platelet count from 99.0±0.52x103/ul to 529±0.01x 103/ul, 332±0.523x103/ul and 135±0.543x103/ul respectively. Conclusion: Ingestion of methanolic leaf extract of Achyranthes aspera resulted in a dose dependent changes in hematological parameter of albino mice including significant increase in lymphocyte and platelet counts. This plant’s leaf extract may therefore have the potentials of being effective in the treatment of diseases cause by thrombocytopenia and lymphocytopenia in mammals. It is believed that information provided in this study can enhance appropriate usage of Achyranthes aspera in folkloric medicine in Nigeria.
    VL  - 4
    IS  - 4
    ER  - 

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Author Information
  • Department of Laboratory Services, Federal Medical Centre, Yola, Adamawa State, Nigeria

  • Department of Laboratory Services, Federal Medical Centre, Yola, Adamawa State, Nigeria

  • Department of University Health Services, Micheal Okpara University, Abia State, Nigeria

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