Background Lead is a common occupational toxicant. Long-term exposure to lead can damage the nervous and hematopoietic systems. Blood lead concentration is an important biomarker for assessing recent lead exposure. Establishing an accurate and sensitive method for blood lead determination is of great significance for occupational health monitoring. Objective To establish and validate an inductively coupled plasma mass spectrometry (ICP-MS) method for the determination of lead in blood. Methods Using an Agilent 7850 ICP-MS instrument, blood samples were directly diluted with a diluent containing 0.5% nitric acid and 0.01% Triton X-100. Holmium (Ho) was used as an online internal standard. Lead was measured at m/z 206, 207, and 208 in standard mode. The method was validated for linearity, detection limit, quantification limit, recovery, and precision according to the national standard GBZ/T 316.2-2018. Results Excellent linearity was observed in the range of 0–100 μg/L, with correlation coefficients (R²) of 1 for all three mass numbers. The detection limit was 0.013 μg/L, and the quantification limit was 0.044 μg/L. Recoveries at low (0.4 μg/L), medium (40.0 μg/L), and high (400.0 μg/L) concentration levels were 96.23%, 102.28%, and 100.64%, respectively, with relative standard deviations (RSD) of 1.10%, 0.45%, and 0.43%. Conclusion The method is simple, sensitive, and reproducible. All performance indicators are superior to the national standard requirements, making it suitable for rapid and accurate determination of lead in blood among occupationally exposed populations.
| Published in | Science Research (Volume 14, Issue 3) |
| DOI | 10.11648/j.sr.20261403.15 |
| Page(s) | 99-104 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2026. Published by Science Publishing Group |
Blood Lead, ICP-MS, Method Validation
参数 | 设计值 |
|---|---|
射频功率 | 1450 W |
冷却气流量（Ar） | 12.5 L/min |
辅助气流量（Ar） | 0.70 L/min |
雾化气流量（Ar） | 0.92 L/min |
蠕动泵转速 | 30 r/min |
雾化器温度 | 3 ℃ |
采样深度 | 8 mm |
测定模式 | 标准模式（无碰撞气体） |
测定质量数 | 206Pb, 207Pb,208Pb |
内标元素 | 165Ho |
内标引入方式 | 在线三通混合 |
内标浓度 | 10.0μg/L |
采集次数 | 3次 |
积分时间 | 0.1 s/质量数 |
每个样品总时间 | 约2 min |
检测项目 | 血铅值（μg/L） |
|---|---|
测定平均值 | 0.3849 |
理论浓度 | 0.4000 |
标准偏差 | 0.0044 |
检出限 | 0.013 |
定量限 | 0.044 |
理论浓度（μg/L） | 实测浓度（μg/L） | 回收率（%） | 精密度（%） |
|---|---|---|---|
0.4 | 0.3849 ± 0.0044 | 96.23 | 1.10 |
40.0 | 40.91 ± 0.18 | 102.28 | 0.45 |
400.0 | 402.57 ±1.70 | 100.64 | 0.43 |
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APA Style
Zhou, X. (2026). Confirmation of the ICP-MS Method for the Determination of Blood Lead. Science Research, 14(3), 99-104. https://doi.org/10.11648/j.sr.20261403.15
ACS Style
Zhou, X. Confirmation of the ICP-MS Method for the Determination of Blood Lead. Sci. Res. 2026, 14(3), 99-104. doi: 10.11648/j.sr.20261403.15
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Download@article{10.11648/j.sr.20261403.15,
author = {Xiaomin Zhou},
title = {Confirmation of the ICP-MS Method for the Determination of Blood Lead},
journal = {Science Research},
volume = {14},
number = {3},
pages = {99-104},
doi = {10.11648/j.sr.20261403.15},
url = {https://doi.org/10.11648/j.sr.20261403.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sr.20261403.15},
abstract = {Background Lead is a common occupational toxicant. Long-term exposure to lead can damage the nervous and hematopoietic systems. Blood lead concentration is an important biomarker for assessing recent lead exposure. Establishing an accurate and sensitive method for blood lead determination is of great significance for occupational health monitoring. Objective To establish and validate an inductively coupled plasma mass spectrometry (ICP-MS) method for the determination of lead in blood. Methods Using an Agilent 7850 ICP-MS instrument, blood samples were directly diluted with a diluent containing 0.5% nitric acid and 0.01% Triton X-100. Holmium (Ho) was used as an online internal standard. Lead was measured at m/z 206, 207, and 208 in standard mode. The method was validated for linearity, detection limit, quantification limit, recovery, and precision according to the national standard GBZ/T 316.2-2018. Results Excellent linearity was observed in the range of 0–100 μg/L, with correlation coefficients (R²) of 1 for all three mass numbers. The detection limit was 0.013 μg/L, and the quantification limit was 0.044 μg/L. Recoveries at low (0.4 μg/L), medium (40.0 μg/L), and high (400.0 μg/L) concentration levels were 96.23%, 102.28%, and 100.64%, respectively, with relative standard deviations (RSD) of 1.10%, 0.45%, and 0.43%. Conclusion The method is simple, sensitive, and reproducible. All performance indicators are superior to the national standard requirements, making it suitable for rapid and accurate determination of lead in blood among occupationally exposed populations.},
year = {2026}
}
TY - JOUR T1 - Confirmation of the ICP-MS Method for the Determination of Blood Lead AU - Xiaomin Zhou Y1 - 2026/06/09 PY - 2026 N1 - https://doi.org/10.11648/j.sr.20261403.15 DO - 10.11648/j.sr.20261403.15 T2 - Science Research JF - Science Research JO - Science Research SP - 99 EP - 104 PB - Science Publishing Group SN - 2329-0927 UR - https://doi.org/10.11648/j.sr.20261403.15 AB - Background Lead is a common occupational toxicant. Long-term exposure to lead can damage the nervous and hematopoietic systems. Blood lead concentration is an important biomarker for assessing recent lead exposure. Establishing an accurate and sensitive method for blood lead determination is of great significance for occupational health monitoring. Objective To establish and validate an inductively coupled plasma mass spectrometry (ICP-MS) method for the determination of lead in blood. Methods Using an Agilent 7850 ICP-MS instrument, blood samples were directly diluted with a diluent containing 0.5% nitric acid and 0.01% Triton X-100. Holmium (Ho) was used as an online internal standard. Lead was measured at m/z 206, 207, and 208 in standard mode. The method was validated for linearity, detection limit, quantification limit, recovery, and precision according to the national standard GBZ/T 316.2-2018. Results Excellent linearity was observed in the range of 0–100 μg/L, with correlation coefficients (R²) of 1 for all three mass numbers. The detection limit was 0.013 μg/L, and the quantification limit was 0.044 μg/L. Recoveries at low (0.4 μg/L), medium (40.0 μg/L), and high (400.0 μg/L) concentration levels were 96.23%, 102.28%, and 100.64%, respectively, with relative standard deviations (RSD) of 1.10%, 0.45%, and 0.43%. Conclusion The method is simple, sensitive, and reproducible. All performance indicators are superior to the national standard requirements, making it suitable for rapid and accurate determination of lead in blood among occupationally exposed populations. VL - 14 IS - 3 ER -
Huizhou Occupational Health Hospital, Huizhou, China
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