HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration

Bushra Munir; Bilal Ahmed; Abdul Ghaffar; Tahira Iqbal; Naila Rafiq; Farah Latif

doi:doi:10.11648/j.sr.20150303.17

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HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration

Bushra Munir, Bilal Ahmed, Abdul Ghaffar, Tahira Iqbal, Naila Rafiq, Farah Latif

Published in Science Research (Volume 3, Issue 3)

Received: 21 February 2015 Accepted: 1 March 2015 Published: 28 May 2015

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Abstract

Pyrazinamide is use for the treatment of tuberculosis in all over the world. Bio kinetics deals with the mathematical description of drug changes in the body with time function. This study was designed to investigate the bio kinetics of Pyrazinamide after oral administration of Pyrazinamide (25mg) tablet in 10 healthy volunteers. The blood samples of each volunteer were collected from 0.5 to 12 hours at different time intervals after the medication. The concentration of Pyrazinamide in plasma samples were determined by high performance liquid chromatography (HPLC). Quantitative observations were recorded that include the mean ± SD value of absorption rate constant (Ka), time to peak (Tmax) and peak concentration (Cmax) were 0.38 ± 0.25/h, 2.248 ± 0.64 h and 4.165 ± 2.13µg/mL, respectively. The mean ± SD values of absorption half-life (t1/2a) and elimination half-life (t1/2b) were 2.078 ± 1.678h and 0.2908 ± 1.721 hours. The mean ± SD values of volume of distribution and total body clearance were 40 ± 27.4L and 3.351 ± 1.27 h, respectively. Mean residue time (MRT) showed mean ± SD and area under curve (AUC) were 17.23 ± 12.78 h and 90.4 ± 76.2 h.mg/L, respectively.

Published in	Science Research (Volume 3, Issue 3)
DOI	10.11648/j.sr.20150303.17
Page(s)	89-92
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Tuberculosis, HPLC, Bio Kinetics, Pyrazinamide

References

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APA Style

Bushra Munir, Bilal Ahmed, Abdul Ghaffar, Tahira Iqbal, Naila Rafiq, et al. (2015). HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration. Science Research, 3(3), 89-92. https://doi.org/10.11648/j.sr.20150303.17

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ACS Style

Bushra Munir; Bilal Ahmed; Abdul Ghaffar; Tahira Iqbal; Naila Rafiq, et al. HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration. Sci. Res. 2015, 3(3), 89-92. doi: 10.11648/j.sr.20150303.17

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AMA Style

Bushra Munir, Bilal Ahmed, Abdul Ghaffar, Tahira Iqbal, Naila Rafiq, et al. HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration. Sci Res. 2015;3(3):89-92. doi: 10.11648/j.sr.20150303.17

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@article{10.11648/j.sr.20150303.17,
  author = {Bushra Munir and Bilal Ahmed and Abdul Ghaffar and Tahira Iqbal and Naila Rafiq and Farah Latif},
  title = {HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration},
  journal = {Science Research},
  volume = {3},
  number = {3},
  pages = {89-92},
  doi = {10.11648/j.sr.20150303.17},
  url = {https://doi.org/10.11648/j.sr.20150303.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sr.20150303.17},
  abstract = {Pyrazinamide is use for the treatment of tuberculosis in all over the world. Bio kinetics deals with the mathematical description of drug changes in the body with time function. This study was designed to investigate the bio kinetics of Pyrazinamide after oral administration of Pyrazinamide (25mg) tablet in 10 healthy volunteers. The blood samples of each volunteer were collected from 0.5 to 12 hours at different time intervals after the medication. The concentration of Pyrazinamide in plasma samples were determined by high performance liquid chromatography (HPLC). Quantitative observations were recorded that include the mean ± SD value of absorption rate constant (Ka), time to peak (Tmax) and peak concentration (Cmax) were 0.38 ± 0.25/h, 2.248 ± 0.64 h and 4.165 ± 2.13µg/mL, respectively. The mean ± SD values of absorption half-life (t1/2a) and elimination half-life (t1/2b) were 2.078 ± 1.678h and 0.2908 ± 1.721 hours. The mean ± SD values of volume of distribution and total body clearance were 40 ± 27.4L and 3.351 ± 1.27 h, respectively. Mean residue time (MRT) showed mean ± SD and area under curve (AUC) were 17.23 ± 12.78 h and 90.4 ± 76.2 h.mg/L, respectively.},
 year = {2015}
}

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TY - JOUR
T1 - HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration
AU - Bushra Munir
AU - Bilal Ahmed
AU - Abdul Ghaffar
AU - Tahira Iqbal
AU - Naila Rafiq
AU - Farah Latif
Y1 - 2015/05/28
PY - 2015
N1 - https://doi.org/10.11648/j.sr.20150303.17
DO - 10.11648/j.sr.20150303.17
T2 - Science Research
JF - Science Research
JO - Science Research
SP - 89
EP - 92
PB - Science Publishing Group
SN - 2329-0927
UR - https://doi.org/10.11648/j.sr.20150303.17
AB - Pyrazinamide is use for the treatment of tuberculosis in all over the world. Bio kinetics deals with the mathematical description of drug changes in the body with time function. This study was designed to investigate the bio kinetics of Pyrazinamide after oral administration of Pyrazinamide (25mg) tablet in 10 healthy volunteers. The blood samples of each volunteer were collected from 0.5 to 12 hours at different time intervals after the medication. The concentration of Pyrazinamide in plasma samples were determined by high performance liquid chromatography (HPLC). Quantitative observations were recorded that include the mean ± SD value of absorption rate constant (Ka), time to peak (Tmax) and peak concentration (Cmax) were 0.38 ± 0.25/h, 2.248 ± 0.64 h and 4.165 ± 2.13µg/mL, respectively. The mean ± SD values of absorption half-life (t1/2a) and elimination half-life (t1/2b) were 2.078 ± 1.678h and 0.2908 ± 1.721 hours. The mean ± SD values of volume of distribution and total body clearance were 40 ± 27.4L and 3.351 ± 1.27 h, respectively. Mean residue time (MRT) showed mean ± SD and area under curve (AUC) were 17.23 ± 12.78 h and 90.4 ± 76.2 h.mg/L, respectively.
VL - 3
IS - 3
ER -

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Author Information

Bushra Munir

Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Bilal Ahmed

Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Abdul Ghaffar

Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Tahira Iqbal

Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Naila Rafiq

Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Farah Latif

Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan

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APA Style

Bushra Munir, Bilal Ahmed, Abdul Ghaffar, Tahira Iqbal, Naila Rafiq, et al. (2015). HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration. Science Research, 3(3), 89-92. https://doi.org/10.11648/j.sr.20150303.17

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ACS Style

Bushra Munir; Bilal Ahmed; Abdul Ghaffar; Tahira Iqbal; Naila Rafiq, et al. HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration. Sci. Res. 2015, 3(3), 89-92. doi: 10.11648/j.sr.20150303.17

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AMA Style

Bushra Munir, Bilal Ahmed, Abdul Ghaffar, Tahira Iqbal, Naila Rafiq, et al. HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration. Sci Res. 2015;3(3):89-92. doi: 10.11648/j.sr.20150303.17

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@article{10.11648/j.sr.20150303.17,
  author = {Bushra Munir and Bilal Ahmed and Abdul Ghaffar and Tahira Iqbal and Naila Rafiq and Farah Latif},
  title = {HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration},
  journal = {Science Research},
  volume = {3},
  number = {3},
  pages = {89-92},
  doi = {10.11648/j.sr.20150303.17},
  url = {https://doi.org/10.11648/j.sr.20150303.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sr.20150303.17},
  abstract = {Pyrazinamide is use for the treatment of tuberculosis in all over the world. Bio kinetics deals with the mathematical description of drug changes in the body with time function. This study was designed to investigate the bio kinetics of Pyrazinamide after oral administration of Pyrazinamide (25mg) tablet in 10 healthy volunteers. The blood samples of each volunteer were collected from 0.5 to 12 hours at different time intervals after the medication. The concentration of Pyrazinamide in plasma samples were determined by high performance liquid chromatography (HPLC). Quantitative observations were recorded that include the mean ± SD value of absorption rate constant (Ka), time to peak (Tmax) and peak concentration (Cmax) were 0.38 ± 0.25/h, 2.248 ± 0.64 h and 4.165 ± 2.13µg/mL, respectively. The mean ± SD values of absorption half-life (t1/2a) and elimination half-life (t1/2b) were 2.078 ± 1.678h and 0.2908 ± 1.721 hours. The mean ± SD values of volume of distribution and total body clearance were 40 ± 27.4L and 3.351 ± 1.27 h, respectively. Mean residue time (MRT) showed mean ± SD and area under curve (AUC) were 17.23 ± 12.78 h and 90.4 ± 76.2 h.mg/L, respectively.},
 year = {2015}
}

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