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Identification of Differentially Expressed Genes During Pseudomonas fluorescens Mediated Systemic Resistance in Cabbage

Published in Plant (Volume 3, Issue 4)
Received: 10 September 2014     Accepted: 31 October 2014     Published: 14 July 2015
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Abstract

The study of microbial ecology and the microbial interactions with plants provides an insight into the biocontrol of plant diseases using antagonistic microbes. Pseudomonas fluorescens was used as a biological control agent against black rot disease caused by Xanthomonas campestris pv. campestris. The Suppression subtractive hybridization (SSH) was used to elucidate the differentially expressed genes in cabbage (Brassica oleracea var. capitata) upon the application of Pseudomonas fluorescens. A total of 140 expressed sequence tags (EST) were obtained. The analyses of these ESTs showed that many defense related genes like peroxidase, heat shock proteins, were upregulated. Many transcripts related to signalling pathways and pathogen recognition were identified. The important finding of the study is the identification of the unigene belonging to the SWEET protein family in cabbage. The study also resulted in the identification of 10 unigenes which possibly depict the interaction of Pseudomonas fluorescens in combating disease. These unigenes have been submitted to dbEST. The results show that those genes which are upregulated during pathogen attack are also induced upon application of Pseudomonas fluorescens indicating the possible mechanism of systemic resistance induced by P. fluorescens to combat disease.

Published in Plant (Volume 3, Issue 4)
DOI 10.11648/j.plant.20150304.11
Page(s) 30-36
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Xanthomonas campestris pv. campestris, RT-PCR, Pseudomonas Fluorescens, Suppression Subtractive Hybridization

References
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[6] Diatchenko L, Lukyanov S, Lau YF, Siebert PD. (1999) Suppression subtractive hybridisation: a versatile method for identifying differentially expressed genes. Methods in Enzymol 303:349–380.
[7] Ganeshmoorthi P, Anand T, Prakasan V, Bharani M, Ragupathi N, Samiyappan R. (2008) Plant growth promoting rhizobacterial (PGPR) bioconsortia mediates induction of defense-related proteins against infection of root rot pathogen in mulberry plants. J Plant Interact 3:233-244.
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  • APA Style

    Kaunain Roohie, Sharanaiah Umesha. (2015). Identification of Differentially Expressed Genes During Pseudomonas fluorescens Mediated Systemic Resistance in Cabbage. Plant, 3(4), 30-36. https://doi.org/10.11648/j.plant.20150304.11

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    ACS Style

    Kaunain Roohie; Sharanaiah Umesha. Identification of Differentially Expressed Genes During Pseudomonas fluorescens Mediated Systemic Resistance in Cabbage. Plant. 2015, 3(4), 30-36. doi: 10.11648/j.plant.20150304.11

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    AMA Style

    Kaunain Roohie, Sharanaiah Umesha. Identification of Differentially Expressed Genes During Pseudomonas fluorescens Mediated Systemic Resistance in Cabbage. Plant. 2015;3(4):30-36. doi: 10.11648/j.plant.20150304.11

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  • @article{10.11648/j.plant.20150304.11,
      author = {Kaunain Roohie and Sharanaiah Umesha},
      title = {Identification of Differentially Expressed Genes During Pseudomonas fluorescens Mediated Systemic Resistance in Cabbage},
      journal = {Plant},
      volume = {3},
      number = {4},
      pages = {30-36},
      doi = {10.11648/j.plant.20150304.11},
      url = {https://doi.org/10.11648/j.plant.20150304.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20150304.11},
      abstract = {The study of microbial ecology and the microbial interactions with plants provides an insight into the biocontrol of plant diseases using antagonistic microbes. Pseudomonas fluorescens was used as a biological control agent against black rot disease caused by Xanthomonas campestris pv. campestris. The Suppression subtractive hybridization (SSH) was used to elucidate the differentially expressed genes in cabbage (Brassica oleracea var. capitata) upon the application of Pseudomonas fluorescens. A total of 140 expressed sequence tags (EST) were obtained. The analyses of these ESTs showed that many defense related genes like peroxidase, heat shock proteins, were upregulated. Many transcripts related to signalling pathways and pathogen recognition were identified. The important finding of the study is the identification of the unigene belonging to the SWEET protein family in cabbage. The study also resulted in the identification of 10 unigenes which possibly depict the interaction of Pseudomonas fluorescens in combating disease. These unigenes have been submitted to dbEST. The results show that those genes which are upregulated during pathogen attack are also induced upon application of Pseudomonas fluorescens indicating the possible mechanism of systemic resistance induced by P. fluorescens to combat disease.},
     year = {2015}
    }
    

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    T1  - Identification of Differentially Expressed Genes During Pseudomonas fluorescens Mediated Systemic Resistance in Cabbage
    AU  - Kaunain Roohie
    AU  - Sharanaiah Umesha
    Y1  - 2015/07/14
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    N1  - https://doi.org/10.11648/j.plant.20150304.11
    DO  - 10.11648/j.plant.20150304.11
    T2  - Plant
    JF  - Plant
    JO  - Plant
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    EP  - 36
    PB  - Science Publishing Group
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    UR  - https://doi.org/10.11648/j.plant.20150304.11
    AB  - The study of microbial ecology and the microbial interactions with plants provides an insight into the biocontrol of plant diseases using antagonistic microbes. Pseudomonas fluorescens was used as a biological control agent against black rot disease caused by Xanthomonas campestris pv. campestris. The Suppression subtractive hybridization (SSH) was used to elucidate the differentially expressed genes in cabbage (Brassica oleracea var. capitata) upon the application of Pseudomonas fluorescens. A total of 140 expressed sequence tags (EST) were obtained. The analyses of these ESTs showed that many defense related genes like peroxidase, heat shock proteins, were upregulated. Many transcripts related to signalling pathways and pathogen recognition were identified. The important finding of the study is the identification of the unigene belonging to the SWEET protein family in cabbage. The study also resulted in the identification of 10 unigenes which possibly depict the interaction of Pseudomonas fluorescens in combating disease. These unigenes have been submitted to dbEST. The results show that those genes which are upregulated during pathogen attack are also induced upon application of Pseudomonas fluorescens indicating the possible mechanism of systemic resistance induced by P. fluorescens to combat disease.
    VL  - 3
    IS  - 4
    ER  - 

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Author Information
  • Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India

  • Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, Karnataka, India

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