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Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes

Published in Plant (Volume 3, Issue 1)
Received: 21 August 2014     Accepted: 9 September 2014     Published: 15 December 2014
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Abstract

Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.

Published in Plant (Volume 3, Issue 1)
DOI 10.11648/j.plant.20150301.11
Page(s) 1-7
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Echinacea Purpurea and Echinacea Pallida, Molecular Marker, PCR, nDNA Antioxidant Activity, Fatty acid, Flavonoid

References
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[9] Goel V, Chang C, Slama JV, Barton R, Bauer R, Gahler R, Basu TK (2002) Alkylamides of Echinacea purpurea stimulate alveolar macrophage function in normal rats. International Immunopharmacology, 2 (2-3:)381-387.
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  • APA Style

    Esra Maltas, Aslı Dageri, Hasibe Cingilli Vural, Salih Yildiz. (2014). Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes. Plant, 3(1), 1-7. https://doi.org/10.11648/j.plant.20150301.11

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    ACS Style

    Esra Maltas; Aslı Dageri; Hasibe Cingilli Vural; Salih Yildiz. Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes. Plant. 2014, 3(1), 1-7. doi: 10.11648/j.plant.20150301.11

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    AMA Style

    Esra Maltas, Aslı Dageri, Hasibe Cingilli Vural, Salih Yildiz. Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes. Plant. 2014;3(1):1-7. doi: 10.11648/j.plant.20150301.11

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  • @article{10.11648/j.plant.20150301.11,
      author = {Esra Maltas and Aslı Dageri and Hasibe Cingilli Vural and Salih Yildiz},
      title = {Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes},
      journal = {Plant},
      volume = {3},
      number = {1},
      pages = {1-7},
      doi = {10.11648/j.plant.20150301.11},
      url = {https://doi.org/10.11648/j.plant.20150301.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20150301.11},
      abstract = {Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Investigation on Biochemical and Molecular Analysis of Echinacea Genotypes
    AU  - Esra Maltas
    AU  - Aslı Dageri
    AU  - Hasibe Cingilli Vural
    AU  - Salih Yildiz
    Y1  - 2014/12/15
    PY  - 2014
    N1  - https://doi.org/10.11648/j.plant.20150301.11
    DO  - 10.11648/j.plant.20150301.11
    T2  - Plant
    JF  - Plant
    JO  - Plant
    SP  - 1
    EP  - 7
    PB  - Science Publishing Group
    SN  - 2331-0677
    UR  - https://doi.org/10.11648/j.plant.20150301.11
    AB  - Genotype identification of medicinal plants remains important for botanical drug industry. Limitations of chemical and morphological approaches for authentication have generated need for newer methods in quality control of botanicals. In this study, the difference between Echinacea purpurea and Echinacea pallida were studied by molecular markers. First of all, genomic DNA from Echinacea genotypes was extracted with EZ1 automatic nucleic acid isolation system and was amplified with OPA primer series. The study showed some relationship between six primers (OPA1, OPA2, OPA3, OPA4, OPA6 and OPA15) with productive performance of Echinacea purpurea and Echinacea pallida. However, secondary metabolites and fatty acids of Echinacea purpurea extract were identified by high performance liquid and gas chromatography, respectively. In addition, antioxidant activity associated with chemical structure of the extract was determined by 1,1 1-diphenyl-2-picrylhydrazyl radical scavenging activity.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey

  • Department of Biology, Faculty of Science, Selcuk University, Konya, Turkey

  • Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey

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