Modern Chemistry

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Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary)

Received: Sep. 27, 2023    Accepted: Oct. 17, 2023    Published: Oct. 31, 2023
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Abstract

One of the plant species that is known for its therapeutic utilize is Salvia Rosmarinus. This experimental study aimed to evaluate anti-diabetic potential, anti-oxidant and antibacterial activities of the plant leave extracts with different solvent having varying polarity. Successive extractions were carried out with petroleum ether, ethyl acetate, and methanol and gave percentage yields of 3.00%, 10.01% and 14.04% respectively. The screening test for metabolites reveals the presence of Alkaloids, Anthraquinones, Phenols, Flavonoids, Steroids, Terpenoids, Saponins, Tannins and Glycosides from different crude extracts under some experimental protocols. The anti-diabetic ability of extract of Salvia Rosmarinus was evaluated by alpha-amylase inhibitory assay which implies that all methanol, ethyl acetate and petroleum ether extracts have greater potential to anti-diabetic activities at a higher concentration. The methanol extract showed the highest anti-diabetic activity from 59.77% to 94.19%, which is very close to the activity of acarbose a standard (72.36% to 95.71%). The ethyl acetate and petroleum ether extracts showed very slow at minimum concentration but rapidly increase their activity when their concentration becomes large from 11.31% to 94.11% and from 6.33% to 76.6% respectively. The total flavonoid concentrations of the petroleum ether, ethyl acetate, and methanol extracts were, respectively, 222.70, 152.1, and 113.71 mg QE/g. And the total phenolic content was 339, 182, and 28 mg GAE/g in methanol, ethyl acetate and petroleum ether extract respectively. Methanolic extract has higher total phenolic and flavonoid content and expected to the highest scavenging activity for free radicals. Lastly, an assessment was conducted on the antibacterial properties of the crude extracts. The highest minimum zone of inhibition was recorded in methanol crude extracts against a negative bacterium E. coli with minimum zone of inhibition 31 + 0.14 mm. Growth inhibition tests against bacteria showed a good result at a higher concentration in methanol and ethyl acetate extracts.

DOI 10.11648/j.mc.20231103.12
Published in Modern Chemistry ( Volume 11, Issue 3, September 2023 )
Page(s) 60-69
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

In Vitro Antibacterial, Anti Diabetic, Agar Disk Diffusion, Rosemary Leave, Phytochemical Screening, Alpha-Amylase Inhibitory Assay

References
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[12] Meresa, A., Gemechu, W., Basha, H., Fekadu, N., Teka, F., Ashebir, R., & Tadele, A. (2017). Herbal medicines for the management of diabetic mellitus in Ethiopia and Eretria including their phytochemical constituents. American Journal of Advanced Drug Delivery, 5(1), 40-58.
[13] Sahira, K. Banu, Cathrine, L (2015). General Techniques Involved in Phytochemical Analysis. International Journal of Advanced Research in Chemical Science (IJARCS), 2: 25-32.
[14] Shrestha, P., Adhikari, S., Lamichhane, B., Govinda, B. S (2015). Phytochemical Screening of the Medicinal Plants of Nepal. IOSR-JESTFT, 1: 11-17.
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[20] Akhtar, M. S., Athar, M. A., & Yaqub, M. (1981). Effect of Momordica charantia on blood glucose level of normal and alloxan-diabetic rabbits. Planta Medica, 42(07), 205-212.
[21] Chelladurai, G. R. M., & Chinnachamy, C. (2018). Alpha amylase and Alpha glucosidase inhibitory effects of aqueous stem extract of Salacia oblonga and its GC-MS analysis. Brazilian Journal of Pharmaceutical Sciences, 54.
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    Tesfahun Dagnaw, Lomi Abayneh, Alebachew Molla, Tangut Masreshaw, Hailemariam Assefa, et al. (2023). Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary). Modern Chemistry, 11(3), 60-69. https://doi.org/10.11648/j.mc.20231103.12

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    ACS Style

    Tesfahun Dagnaw; Lomi Abayneh; Alebachew Molla; Tangut Masreshaw; Hailemariam Assefa, et al. Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary). Mod. Chem. 2023, 11(3), 60-69. doi: 10.11648/j.mc.20231103.12

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    AMA Style

    Tesfahun Dagnaw, Lomi Abayneh, Alebachew Molla, Tangut Masreshaw, Hailemariam Assefa, et al. Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary). Mod Chem. 2023;11(3):60-69. doi: 10.11648/j.mc.20231103.12

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  • @article{10.11648/j.mc.20231103.12,
      author = {Tesfahun Dagnaw and Lomi Abayneh and Alebachew Molla and Tangut Masreshaw and Hailemariam Assefa and Liyew Yizengaw},
      title = {Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary)},
      journal = {Modern Chemistry},
      volume = {11},
      number = {3},
      pages = {60-69},
      doi = {10.11648/j.mc.20231103.12},
      url = {https://doi.org/10.11648/j.mc.20231103.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.mc.20231103.12},
      abstract = {One of the plant species that is known for its therapeutic utilize is Salvia Rosmarinus. This experimental study aimed to evaluate anti-diabetic potential, anti-oxidant and antibacterial activities of the plant leave extracts with different solvent having varying polarity. Successive extractions were carried out with petroleum ether, ethyl acetate, and methanol and gave percentage yields of 3.00%, 10.01% and 14.04% respectively. The screening test for metabolites reveals the presence of Alkaloids, Anthraquinones, Phenols, Flavonoids, Steroids, Terpenoids, Saponins, Tannins and Glycosides from different crude extracts under some experimental protocols. The anti-diabetic ability of extract of Salvia Rosmarinus was evaluated by alpha-amylase inhibitory assay which implies that all methanol, ethyl acetate and petroleum ether extracts have greater potential to anti-diabetic activities at a higher concentration. The methanol extract showed the highest anti-diabetic activity from 59.77% to 94.19%, which is very close to the activity of acarbose a standard (72.36% to 95.71%). The ethyl acetate and petroleum ether extracts showed very slow at minimum concentration but rapidly increase their activity when their concentration becomes large from 11.31% to 94.11% and from 6.33% to 76.6% respectively. The total flavonoid concentrations of the petroleum ether, ethyl acetate, and methanol extracts were, respectively, 222.70, 152.1, and 113.71 mg QE/g. And the total phenolic content was 339, 182, and 28 mg GAE/g in methanol, ethyl acetate and petroleum ether extract respectively. Methanolic extract has higher total phenolic and flavonoid content and expected to the highest scavenging activity for free radicals. Lastly, an assessment was conducted on the antibacterial properties of the crude extracts. The highest minimum zone of inhibition was recorded in methanol crude extracts against a negative bacterium E. coli with minimum zone of inhibition 31 + 0.14 mm. Growth inhibition tests against bacteria showed a good result at a higher concentration in methanol and ethyl acetate extracts.
    },
     year = {2023}
    }
    

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  • TY  - JOUR
    T1  - Anti-Diabetic, Anti-Oxidant and Antibacterial Activity of Leaves Extract of Salvia Rosmarinus (Rosemary)
    AU  - Tesfahun Dagnaw
    AU  - Lomi Abayneh
    AU  - Alebachew Molla
    AU  - Tangut Masreshaw
    AU  - Hailemariam Assefa
    AU  - Liyew Yizengaw
    Y1  - 2023/10/31
    PY  - 2023
    N1  - https://doi.org/10.11648/j.mc.20231103.12
    DO  - 10.11648/j.mc.20231103.12
    T2  - Modern Chemistry
    JF  - Modern Chemistry
    JO  - Modern Chemistry
    SP  - 60
    EP  - 69
    PB  - Science Publishing Group
    SN  - 2329-180X
    UR  - https://doi.org/10.11648/j.mc.20231103.12
    AB  - One of the plant species that is known for its therapeutic utilize is Salvia Rosmarinus. This experimental study aimed to evaluate anti-diabetic potential, anti-oxidant and antibacterial activities of the plant leave extracts with different solvent having varying polarity. Successive extractions were carried out with petroleum ether, ethyl acetate, and methanol and gave percentage yields of 3.00%, 10.01% and 14.04% respectively. The screening test for metabolites reveals the presence of Alkaloids, Anthraquinones, Phenols, Flavonoids, Steroids, Terpenoids, Saponins, Tannins and Glycosides from different crude extracts under some experimental protocols. The anti-diabetic ability of extract of Salvia Rosmarinus was evaluated by alpha-amylase inhibitory assay which implies that all methanol, ethyl acetate and petroleum ether extracts have greater potential to anti-diabetic activities at a higher concentration. The methanol extract showed the highest anti-diabetic activity from 59.77% to 94.19%, which is very close to the activity of acarbose a standard (72.36% to 95.71%). The ethyl acetate and petroleum ether extracts showed very slow at minimum concentration but rapidly increase their activity when their concentration becomes large from 11.31% to 94.11% and from 6.33% to 76.6% respectively. The total flavonoid concentrations of the petroleum ether, ethyl acetate, and methanol extracts were, respectively, 222.70, 152.1, and 113.71 mg QE/g. And the total phenolic content was 339, 182, and 28 mg GAE/g in methanol, ethyl acetate and petroleum ether extract respectively. Methanolic extract has higher total phenolic and flavonoid content and expected to the highest scavenging activity for free radicals. Lastly, an assessment was conducted on the antibacterial properties of the crude extracts. The highest minimum zone of inhibition was recorded in methanol crude extracts against a negative bacterium E. coli with minimum zone of inhibition 31 + 0.14 mm. Growth inhibition tests against bacteria showed a good result at a higher concentration in methanol and ethyl acetate extracts.
    
    VL  - 11
    IS  - 3
    ER  - 

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Author Information
  • Department of Chemistry, Wolaita Sodo University, Wolaita Sodo City, Ethiopia

  • Department of Chemistry, Wolaita Sodo University, Wolaita Sodo City, Ethiopia

  • Department of Biotechnology, Wolaita Sodo University, Wolaita Sodo City, Ethiopia

  • Department of Physics, Wolaita Sodo University, Wolaita Sodo City, Ethiopia

  • Department of Chemistry, Mekdela Amba University, Mekaneselam City, Ethiopia

  • Department of Chemistry, College of Natural and Computational Science, MizanTepi University, Mizan, Ethiopia

  • Section